Printer Friendly

Breaking the oligonucleotide jam.

Clarity OTX extraction kits from Phenomenex Inc., Torrance, Calif., speed up the isolation of RNA and DNA-based therapeutics and their metabolites from ADME/pharmacokinetics studies in preparation for downstream LC/MS analysis. While traditional methods require eight steps and can take two hours, the Clarity OTX extraction kits isolate oligonucleotide therapeutics and their metabolites in 15 minutes. It can be used for both plasma and tissue; other methods require separate procedures for each sample type.

[ILLUSTRATION OMITTED]

The solutions combine a solid phase extraction (SPE) sorbent with buffers to deliver high recovery of all types of oligo therapeutics. The kit solution can be scaled up to a 96-well plate format for automated or parallel processing for high-throughput environments.

The Clarity OTX protocol uses a propriety mixture of chaotropes, detergents, and other dispersants to disrupt intermolecular interactions that are common for oligonucleotides in biological matrices. A proprietary mixed-mode sorbent captures and retains small synthetic oligonucleotide-based therapeutics.

* Phonomenex Inc., www.phenomenex.com

COPYRIGHT 2010 Advantage Business Media
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2010 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:Life Sciences; Clarity OTX
Publication:R & D
Article Type:Brief article
Geographic Code:1USA
Date:Sep 1, 2010
Words:160
Previous Article:Bringing spectroscopy cell studies to life.
Next Article:3d system visualizes healthy knees.
Topics:

Terms of use | Privacy policy | Copyright © 2019 Farlex, Inc. | Feedback | For webmasters