Boehringer Ingelheim obtains United States patent.
University of Guelph (Guelph, Canada) has patented a method for
embryogenesis and a method for plant regeneration. Microspore-containing
plant segment from donor plants are harvested and incubated under
pre-treatment conditions to maintain microspore at a uninucleate cell
cycle G1 phase. Pre-treatment conditions comprise cold water or an
aqueous solution of about 0.2-about 1.0 mol/liter sugar alcohol, for
example mannitol. Microspores are isolated from the plant segment and
embryogenesis of microspores is induced in induction medium, thereby
producing embryos. Green plants may be regenerated from the embryos
produced. Arabinogalactan protein, auxin and ovary co-culture may be
added to the induction medium to enhance embryogenesis from microspores.