Base deficit as an early marker of coagulopathy in trauma.
The coagulopathy of trauma is associated with an increased mortality rate,  and the key to improving survival lies in identifying and correcting early potentially reversible risk factors. Although traditionally hypoperfusion has been diagnosed and classified on the basis of clinical vital signs, occult hypoperfusion may occur in the presence of normal haemodynamic parameters. The measurement of lactate, the product of anaerobic metabolism, and the surrogate base deficit (BD) are well-recognised markers of cellular hypoperfusion and the severity of shock,  although BD may also be elevated under aerobic conditions such as acute kidney injury or hyperchloraemia, both of which commonly occur after major trauma as a result of renal hypoperfusion and excessive saline-containing resuscitation fluids. Both lactate and BD have been extensively investigated in trauma patients as markers of injury severity, end-points of resuscitation and predictors of outcome.
Although indicating the severity of hypoperfusion, measurement of lactate alone may not portray the extent of the metabolic acidosis. BD quantifies the extent of both anaerobic and aerobic acidoses and may be a better indicator of the risk of a coagulation disturbance. This study was undertaken to identify the correlation between BD, lactate and injury severity with the development of early-onset coagulopathy and mortality.
The study was approved by the Biomedical Research Ethics Committee (BE207/09) of the University of KwaZulu-Natal, Durban, South Africa. A retrospective chart analysis of 75 trauma patients admitted directly from the scene of injury to the level I trauma unit at Inkosi Albert Luthuli Central Hospital, Durban, from 2007 to 2008 was performed and data pertaining to patients with evidence of hypoperfusion were extracted. Hypoperfusion was defined as a BD >-2 (for the purpose of this study the symbol 'greater than' describes a more negative BD) and coagulopathy as an International Normalized Ratio (INR) of >1.2. All blood results were those taken on admission to the resuscitation room via an arterial sample. The BD was obtained from arterial blood gas analysis. Arterial lactate, chloride ion concentration and INR results were extracted from the Medicom electronic hospital laboratory database. Tympanic membrane temperature was recorded on admission to the resuscitation room. The Injury Severity Score (ISS) was computed after all injuries had been identified using the Abbreviated Injury Scale (AIS) 90 reference book. Death or survival was ascertained from the electronic hospital discharge notes. Complete data were available for 28 patients and were included in the analysis.
Student's t-test and Fisher's exact test were used for continuous and categorical variables, respectively. Correlation curves were used to determine the degree of association between the variables BD, lactate and ISS with respect to the INR. A p-value of <0.05 was considered statistically significant.
Of the 28 patients, 18 were males (64.3%) and 10 females (35.7%). The mean age was 31 years (range 1-75 years, median 30 years). The mechanism of injury was penetrating trauma in 5 cases (17.9%) and blunt trauma in 23 (82.1%). The median ISS was 24 (range 4-59). In 16 patients (57.1%) the INR was within normal limits, but in 12 (42.9%) it was >1.2. The differences in mean BD, lactate, INR, ISS, systolic blood pressure and temperature between these two groups are shown in Table 1.
Lactate and BD showed a strong and significant correlation (r=0.739; p<0.001). There was a moderate and significant correlation between BD and INR (r=0.393; p=0.019), and the ISS demonstrated a stronger significant association (r=0.565; p<0.001). Lactate showed a weak and non-significant association with the INR (r=0.232; p=0.18). Chloride was above the upper limit of normal in 17 patients (60.7%), although there was no significant difference between the coagulopathic and non-coagulopathic patients.
There were a total of 12 deaths (42.8%) in this cohort of patients with biochemical evidence of hypoperfusion. In the group with coagulopathy 9/12 (75.0%) died, compared with only 3/16 (18.7%) of those with a normal INR (p=0.006).
Metabolic acidosis has been incriminated as one of the instigators of coagulation disturbances following injury. The BD is defined as the amount of milli-equivalents of base required to titrate a litre of blood to a pH of 7.4 at an arterial partial pressure of carbon dioxide (PaC[O.sub.2]) of 40 mmHg.  This measurement is unaffected by acute changes in PaC[O.sub.2], is universally elevated in the presence of all pathological changes that induce a metabolic acidosis, and as such is a more reliable marker of the severity of the underlying metabolic state than pH, which will be altered by respiratory compensation. In the trauma setting, increased lactate production arises from anaerobic metabolism as a consequence of hypoperfusion, most commonly due to haemorrhage, but will not be affected by a metabolic acidosis that arises under aerobic conditions. Despite the fact that the BD not only reflects the degree of lactate accumulation but also provides a more composite view of a patient's metabolic status, [6,7] lactate clearance has been more popular than BD as a predictor of mortality in the trauma population.  Davis  showed a strong correlation between BD and lactate in a porcine haemorrhagic shock model and concluded that the BD was as accurate a marker of tissue hypoperfusion as lactate.The admission BD has also been shown to correlate well with the ISS, development of multiple organ failure, length of intensive care unit and hospital stay, need for blood transfusions and mortality/101 and a BD of [greater than or equal to] 6 has been shown to be a marker of injury severity and mortality in the trauma population, irrespective of age. [3,10,11]
Although hypoperfusion and the subsequent lactic acidosis play a key role in the pathogenesis of the early coagulopathy of trauma, the addition of another mechanism for metabolic acidosis will accentuate the risk. Our results show a moderate but significant correlation between an elevated BD and coagulopathy as measured by the INR, but we could not demonstrate a similar association with lactate despite the BD and lactate showing a strong correlation. As mentioned above, the reason may lie in the fact that lactate only reflects an acidosis arising from anaerobic metabolism, whereas the BD is a global marker of both anaerobic and aerobic acidoses. In addition to (and as a consequence of) hypoperfusion, acute kidney injury may contribute to the acidosis of injury over and above that from anaerobic metabolism. Hyperchloraemia is a common consequence of kidney injury, and although previously thought innocuous has a detrimental effect on outcome when combined with another acidosis.  Furthermore, hyperchloraemia may not only contribute to an acidotic state during renal insufficiency, but aggravate renal damage further by inducing renal vasoconstriction. [13,14] Given the incidence of hyperchloraemia in our patient population, which in addition to hypoperfusion may contribute to metabolic acidosis, acute kidney injury and coagulopathy, the BD may be a more useful predictor of the risk of coagulation disturbances and an indicator for the early use of plasma rather than clear fluids for resuscitation.
The ISS showed the strongest association with an elevated INR. Although hypoperfusion is undoubtedly a contributing factor in the coagulopathy of trauma, it is not a prerequisite. Tissue factor exposure has been incriminated as a major trigger mechanism, [2,15] and it may be assumed that a higher ISS would be associated with more severe tissue damage, an increase in coagulation disturbances and a higher mortality rate. The presence of coagulopathy at admission is associated with increased injury severity, length of hospital stay and number of organ failures and mortality, and we have shown a statistically significant increase in mortality in our cohort of patients with evidence of hypoperfusion and who were coagulopathic on admission.
In the acute resuscitation phase, acquiring an INR result may take up to an hour. In the emergency setting, thromboelastometry has been shown to be a quick, useful method in identifying patients with coagulopathy and guiding transfusion requirements. A strong correlation has been shown between thromboelastometry and derangements in conventional clotting parameters, results are available within 10 minutes, and this simple device has now gained acceptance as standard of care in the severely injured.
It is imperative to identify patients with acute coagulopathy early as this may influence outcome and resuscitation efforts. The BD can be obtained within minutes using a conventional blood gas analyser, which is available in most emergency departments, and has a significant association with acute coagulation disturbances. This finding could have far-reaching clinical applications. In addressing hypoperfusion in the resuscitation bay, the conventional use of crystalloids or colloids could be replaced by the immediate use of freeze-dried plasma that would lead to early replenishment of clotting factors in addition to sustained volume expansion. The recent war in Iraq has given rise to similar opinions, suggesting that fresh-frozen plasma be used as the resuscitation fluid of choice in the acute combat setting to decrease additional bleeding and replenish clotting factor deficiencies.
There are several limitations to our study. The sample may be too small in size and not fully representative of the trauma population. There are major discrepancies in the literature with regard to defining coagulopathy, and the INR value of >1.2 that we used may be too sensitive and overestimate the presence of coagulopathy in the study group. In a review by Brohi et al., four major studies used four different indices to define coagulopathy. An estimate of pre-hospital fluid administration was lacking from our database and was not included in our assessment. Even considering these shortfalls, there is a growing body of evidence indicating that in order to improve survival in the seriously injured we need to address the possibility of a coagulation disturbance much earlier than was previously thought. The simple combination of BD and an estimate of the ISS will help to identify those at risk.
Our findings suggest that a significant correlation exists between BD, ISS and INR and that BD is superior to lactate in predicting coagulation disturbances. The combination of hypoperfusion and a coagulopathy at admission is associated with a significant increase in mortality.
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S Cheddie, MMed (Surg), FCS (SA); D J J Muckart, FRCS, MMSc; T C Hardcastle, MMed, FCS (SA)
Department of General Surgery, Nelson R Mandela School of Medicine, University of KwaZulu-Natal, Durban, South Africa
Corresponding author: D J J Muckart (firstname.lastname@example.org)
Table 1. Comparison of coagulopathic v. non-coagulopathic patients Coagulopathy No coagulopathy (mean[+ or -]SD) (mean[+ or -]SD) (n=12) (n=16) Lactate (mmol/l) 7.04[+ or -]7.18 3.69[+ or -]2.98 BD (mmol/l) -9.46[+ or -]4.47 -5.82[+ or -]2.50 INR 1.58[+ or -]0.17 1.08[+ or -]0.07 Temperature (oC) 35.9[+ or -]1.1 36.3[+ or -]1.2 Systolic BP (mmHg) 110.4[+ or -]41.8 122.6[+ or -]27.2 ISS 32.2[+ or -]16.2 17.8[+ or -]9.8 p-value Lactate (mmol/l) 0.10 BD (mmol/l) 0.011 INR <0.001 Temperature (oC) 0.34 Systolic BP (mmHg) 0.36 ISS 0.007 SD = standard deviation; BD = base deficit; INR = International Normalized Ratio; BP = blood pressure; ISS = Injury Severity Score.
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|Title Annotation:||GENERAL SURGERY|
|Author:||Cheddie, S.; Muckart, D.J.J.; Hardcastle, T.C.|
|Publication:||South African Journal of Surgery|
|Article Type:||Clinical report|
|Date:||Aug 1, 2013|
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