Assessment of magnesium status in type 2 diabetes mellitus.
Type 2 DM is a lifelong disease. India leads the world in its largest number of diabetes subjects as compared with other given country. It has been estimated that presently 19.4 million individuals are affected by diabetes and the numbers are expected to increase to 57.2 million by the year 2025 (1/6th of world total).(1)
According to the results of many longitudinal and cross-sectional studies, it has been demonstrated that the earliest detectable abnormality in NIDDM is impairment in body ability to respond to insulin.(2) The metabolic disorder is characterized by abnormal hyperglycemia with disturbance of carbohydrate, fat and protein metabolism which result from defects in insulin secretion, insulin action or both. An alteration in the metabolism of minerals was demonstrated in many cases of diabetes. (3,4,5)
In human, diabetes causes disturbances in the magnesium metabolism. Magnesium has an important role in phosphorylation reaction of glucose and its metabolism. Its deficiency has been implicated in insulin resistance, carbohydrate intolerance, dyslipidemia, etc. It is generally believed that a strict metabolism control delays the development of diabetes mellitus.(6) Magnesium depletion has a negative impact on glucose homeostasis and insulin sensitivity in patients with type 2 DM. Magnesium depletion has been linked to the development of retinopathy thrombosis and hypertension. (7,8,9)
Magnesium intake was inversely associated with incidence of diabetes in young American adults.(10) Clinically, hypomagnesaemia may be defined as serum [Mg.sup.++] concentration <1.6mg/dL or SD[+ or -]2 below the mean of the general population, which can be corrected by magnesium supplements.(11,12) Hence in this study, we evaluated serum magnesium in type 2 diabetes to know the magnesium status of these patients visiting to SHKM Govt. Medical College.
MATERIALS AND METHODS
The study was approved by the Institutional Ethics Committee. A written informed consent was obtained from all participants in this study.
A total of 50 patients with type 2 diabetes mellitus, who attended medicine department in SHKM Govt. Medical College were recruited during year 2014-15. The diagnosis of type 2 diabetes mellitus was confirmed by biochemical investigations as per WHO criteria.(13) Fifty age and sex matched apparently healthy individuals with normal plasma glucose and with no symptoms suggestive of DM were taken as controls in this study.
They were divided into two groups.
Group I- Type 2 diabetes mellitus patients (Diagnosed on the basis of biochemical investigation as per WHO criteria) with normal renal function were included. Group II- Control group which comprised of healthy individuals who had normal blood glucose level and also had normal renal function.
The patients with acute and chronic diarrhoea/malabsorption states with thyroid and adrenal dysfunction, history of alcohol intake, history of vitamin or mineral supplements in the recent past, recent metabolic acidosis, pregnancy, lactation with serum creatinine >1.5mg/dL and on drugs known to affect magnesium levels were excluded from the study.
Fasting venous blood samples were collected from cases and controls and the samples were centrifuged, serum was separated and stored at 40C. Fasting plasma glucose was estimated by using commercially available kit in automated analyzer. Fully automatic analyzer "Roche Evo 500" was used for serum magnesium measurement. For serum magnesium evaluation, Magnesium Xylidyl Blue Method which is based on Serum Magnesium ions reaction with Xylidyl Blue in an alkaline solution to produce a red complex that is measured spectrophotometrically.(14) The intensity of color produced is directly proportional to magnesium concentration. Calcium interference is virtually eliminated by the use of EGTA and a surfactant system is included to remove protein interference.
The Statistical software SPSS 15.0 was used for the analysis of the data and Microsoft Excel have been used to generate graphs and tables. Chi square test and Student 't test have been used to find the significance of study parameters between two groups of subjects. Results were expressed as mean+SD. Probability values of P<0.05 were considered to indicate statistical significance.
The comparative controlled study consisting of 50 diabetics without any complications and 50 non-diabetic healthy subjects were taken to investigate the pattern of serum magnesium in the mentioned groups. The average age of the subjects in the control group was 52[+ or -]11.46, while in experimental group (Group I) was 54[+ or -]10.06 (Table 1). Among all the groups the gender distribution was of the same, i.e. 50% and 50% males and females respectively. The average range of FBS in diabetic was 153.46[+ or -]22.49mg/dL. The mean levels healthy subjects were 79.63[+ or -]5.85mg/dL (Table 2). The difference was statistically significant (P value <0.001).
There is significant difference between levels of serum magnesium levels among diabetics and healthy subjects. The mean serum magnesium levels among the group I was 1.46[+ or -]0.23mg/dL, while in group II was 2.02[+ or -]0.21mg/dL respectively (p<0.001) (Table 2). The serum magnesium level was lower in the type 2 diabetic than healthy subjects (Fig. 1).
Reduced magnesium level may be the result of enhanced renal magnesium excretion. Insulin has been related to magnesium reabsorption at the thick ascending loop of Henle. Insulin deficiency or resistance can promote magnesium wasting at this nephron segment.(8,15) Insulin seems to be one of the most important factors that regulate plasma and intracellular magnesium concentration. It has been suggested that ATPase dependent pump is involved in the mechanism by which insulin regulates erythrocyte magnesium content.(16) In states of insulin resistance, insulin induced entry of magnesium is impaired into the cells.
Available data suggests that low [Mg.sup.++] level may promote endothelial cell dysfunction and thrombogenesis by increasing platelet aggregation and vascular calcification.(17) Low circulating magnesium levels have been related to elevated blood pressure, atherogenic dyslipidemia, impaired clotting, increased inflammatory burden, oxidative stress, carotid wall thickness and coronary heart disease.(18,19)
Decreased magnesium level is a risk factor and favours diabetic complications. Magnesium replacement by promoting dietary replacement and with nutritional medicinal supplements may be a healthy lifestyle to delay the onset of diabetes and its complications.
In this study, we found serum magnesium level of type 2 diabetics significantly lower than healthy control subjects (P<0.001). Low serum magnesium in type 2 DM were also reported in previous studies.(16,20,21)
In this study, we found serum magnesium level of type 2 diabetics significantly lower than healthy control subjects (P<0.001). Low magnesium levels may be the risk factor for the associated increased inflammation, oxidative stress, hypercoagulability and dyslipidemia in type 2 diabetes. The estimation of serum magnesium levels is helpful to monitor the severity of complications in type 2 diabetes and also be useful for proper medical intervention. Magnesium supplementation or dietary intake of magnesium rich food is beneficial to avoid and delay complication of type 2 DM.
[FIGURE 1 OMITTED]
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Sangeeta Singh , Neetu Arora , Seema Shah , Vivek Sharma , Vijay Shankar 
 Associate Professor, Department of Biochemistry, SHKM, Government Medical College, Nalhar, Nuh, Mewat, Haryana.
 Associate Professor, Department of Anatomy, SHKM, Government Medical College, Nalhar, Nuh, Mewat, Haryana.
 Associate Professor, Department of Biochemistry, SHKM, Government Medical College, Nalhar, Nuh, Mewat, Haryana.
 Demonstrator, Department of Biochemistry, SHKM, Government Medical College, Nalhar, Nuh, Mewat, Haryana.
 Professor, Department of Biochemistry, SHKM, Government Medical College, Nalhar, Nuh, Mewat, Haryana.
Financial or Other, Competing Interest: None. Submission 06-01-2016, Peer Review 06-02-2016, Acceptance 12-02-2016, Published 29-02-2016.
Corresponding Author: Dr. Neetu Arora, D-5/11, Ground Floor, Ardee City, Sector-52, Gurgaon-122011.
Table 1: Age and Sex Distribution of the Study Population Group Number Age (Mean [+ or -] SD) Male Female Group I (Diabetics) 50 54 [+ or -] 10.6 25 25 Group II (Control) 50 52 [+ or -] 11.46 25 25 Table 2: Comparison of FBS & Serum Magnesium Level in Diabetic Patients was Significantly Lower (P<0.001) than the Control N Control FBS (mg/dL) 50 79.54 [+ or -] 5.88 Serum Mg 50 2.02 [+ or -] 0.21 Diabetics P value FBS (mg/dL) 153.46 [+ or -] 22.49 <0.001 Serum Mg 1.46 [+ or -] 0.23 <0.001 Fig. 2: Showing Serum Magnesium Level in Two Groups control 2.01 Diabetics 1.458 Note: Table made from bar graph.
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|Title Annotation:||Original Article|
|Author:||Singh, Sangeeta; Arora, Neetu; Shah, Seema; Sharma, Vivek; Shankar, Vijay|
|Publication:||Journal of Evolution of Medical and Dental Sciences|
|Date:||Feb 29, 2016|
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