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Aspects of the field ecology of Stenoma catenifer (Lepidoptera: Elachistidae) infesting Hass avocados in Guatemala.

Stenoma catenifer Walsingham (Lepidoptera: Elachistidae) is native to neotropical areas and is an important pest of avocados Persea americana Miller (Lauraceae) in Mexico, and Central and South America (Wysoki et al. 2002). The principal economic loss to S. catenifer is caused by larvae feeding internally on fruit pulp and seeds which disfigures fruit and can promote premature fruit drop (Nunez 2008). Heavy infestations of stem-mining larvae can kill twigs and young avocado trees (Nunez 2008; Wolfenbarger & Colburn 1979). Larvae pupate within the top 0.5-2.0 cm of soil (Boscan de Martinez & Godoy 1984). Adult S. catenifer moths are nocturnal and hide on the ground during the day (Cervantes Peredo et al. 1999). Eggs are parasitized by trichogrammatids (Hohmann et al. 2003), and larvae are attacked by braconid and ichneumonid parasitoids (Cervantes Peredo et al. 1999; Hoddle & Hoddle 2008; Nava et al. 2005).

Field infestation rates by S. catenifer in commercial Hass avocado orchards can average 45% (Hoddle & Hoddle 2008). California is the largest producer of Hass avocados in the U.S.A., and the industry in California does not have specialist avocado fruit feeding insect pests. Thus, large-scale imports of Hass avocados that originate in the home range of S. catenifer are viewed as a major biosecurity threat to California because movement of infested fruit has been identified as the major conduit for moving this pest into new areas (Nunez 2008).

To better understand the field ecology of S. catenifer during a 6-week field study conducted in a commercial Hass avocado orchard in Guatemala, we investigated (1) the natural enemy fauna associated with S. catenifer larvae, (2) the diurnal flight behavior of deliberately released moths, and (3) the predation risk to S. catenifer larvae, pupae, and adults from lycosid spiders.

A commercial Hass avocado orchard (approximately 5 ha) consisting of about 800 trees in San Miguel Duenas, Sactepequez, Guatemala (N14[degrees]31.461; W90[degrees]46.579; elevation 1500 m) was used for this study over the period Nov 13, 2007 to Dec 21, 2007. Trees in the Hass orchard were about 7 years old, around 5-6 m in height, and separated by 5 to 7 m of clear ground allowing full sun exposure. The orchard was treated monthly with a rotating schedule of malathion and endosulfan. A total of 561 fruit showing external signs of S. catenifer damage were removed from the study orchard from Nov 13 to 27, 2007.

Harvested fruit were held in the laboratory for 10-14 d in insect rearing cages (BugDorm-2120, 60 cm x 60 cm x 60 cm; from MegaView Science Education Services, Taiwan) at 22.56[degrees]C [+ or -] 0.19 and RH 71.33% [+ or -] 4.89 under L:D 12:12. Fruit were inspected daily, and emerged mature S. catenifer larvae were isolated and kept in labeled, ventilated plastic cups. After 10-14 d, all fruit from a particular harvest date were opened, and avocado seeds showing S. catenifer activity were isolated individually. All rearing cups were observed daily until the fate of larvae was recorded as having pupated or died from parasitism or unknown causes.

Survivorship of larvae to adulthood was 38% (Table 1). Two species of primary hymenopterous parasitoid were reared from larvae. A gregarious Apanteles sp. (Braconidae: Microgastrinae) (UCRC ENT 149607-149611) parasitized 60% of S. catenifer larvae. The sex ratio of Apanteles sp. was 64% female, and 93% of parasitoids emerged successfully from cocoons. The mean number of cocoons per host was 7.51 [+ or -] 0.28 (range = 1 to 13).

A solitary Macrocentrus sp. (Braconidae: Macrocentrinae) (UCRC ENT 101108-101111) was reared from 40% of the larvae. The sex ratio for Macrocentrus sp. was 67% female; 80% of parasitoids emerged successfully from cocoons, and 17% of larvae failed to spin cocoons and died. A solitary hyperparasitoid, Perilampus sp. (Hymenoptera: Perilamidae), was reared from 0.7% of Apanteles sp. pupae (UCRC ENT 101112-101114), and 5% of Macrocentrus sp. pupae (UCRC ENT 147087-147088).

On 3 separate occasions (Dec 15,17, and 20, 2007 in the mid-afternoon), a cumulative total of 45 adult S. catenifer reared from field collected larvae were returned to the study orchard, and released individually in 1 of 3 different areas: (1) open ground between trees (n = 15), (2) in the shade immediately beneath the canopy of an avocado tree (n = 15) at a height of ~1.25 m, and (3) at a height of ~3.5 m inside the tree canopy (n = 15). Upon release, each moth was tracked visually to its resting place, and the linear distance flown from release point to final resting place was measured.

Regardless of release site, all adult moths immediately flew to the ground and hid. The mean distance flown was 3.17 m [+ or -] 0.13 (range 1-12 m), and 73% of moths flew [less than or equal to]3 m, and were extremely recalcitrant to movement when probed after landing.

A spider, Hogna sp. (Araneae: Lycosidae), was a common predator on the orchard floor. The potential impact this spider could have on fifth instar S. catenifer seeking pupation sites, exposed pupae, or resting adults was investigated in the laboratory. Twenty spiders were captured in the field, held individually in ventilated plastic vials, and starved for 24 h. After this starvation period, each of 5 randomly selected spiders was either provisioned with a single fifth instar S. catenifer larva, pupa, or adult moth. Five starved spiders were held as controls. After an 8-h period, spider survivorship was assessed, and all spiders provisioned with a S. catenifer life stage were held for an additional 36 h to determine survivorship post-exposure to prey.

All life stages of S. catenifer encountered by starved Hogna sp. (UCRC ENT 138337) were attacked immediately upon introduction into vials. All S. catenifer larvae (n = 5) and adults (n = 5) were consumed, and 4 out of 5 pupae were eaten. All spiders that fed on S. catenifer were still alive 36 h post-exposure. Four of 5 control spiders also survived the experiment.

Hector Leal and Oscar Coy provided unlimited access to the Hass avocado orchard used in this study. John Luhman, John Heraty, and Serguei Triapitsyn identified parasitoids. Tom Prentice identified the Hogna sp. This work was supported in part by the California Avocado Commission.

SUMMARY

Larvae of Stenoma catenifer reared from Hass avocados in Guatemala were parasitized by 2 species of primary parasitoid, Apanteles sp. and Macrocentrus sp. Observations of the diurnal flight activity of adult S. catenifer indicated that 73% of moths fly less than 3 m during the day. Laboratory experiments demonstrated that lycosid spiders are voracious predators of fifth instar larvae, pupae, and adults.

REFERENCES CITED

BOSCAN DE MARTINEz, N., AND F. J. GODOY 1984. Observaciones preliminares sobre la biologia de Stenoma catenifer Walsingham (Lepidoptera: Stenomidae) taladrador del aguacate (Persea americana Mill.). Agron. Trop. 34: 205-208.

CERVANTES PEREDO, L., C. H. C. LYAL, AND V. K. BROWN. 1999. The stenomatine moth, Stenoma catenifer Walsingham: a pre-dispersal seed predator of Greenheart (Chlorocardium rodiei [Schomb.] Rohwer, Richter and van de Werff) in Guyana. J. Nat. Hist. 33: 531-542.

HODDLE, M. S., AND C. D. HODDLE. 2008. Bioecology of Stenoma catenifer Walsingham (Lepidoptera: Elachistidae) and associated larval parasitoids reared from Hass avocados in Guatemala. J. Econ. Entomol. 101: 692-698.

HOHMANN, C. L., A. M. MENEGUIM, E. A. ANDRADE, T. GARCIA DE NOVAES, AND C. ZANDONA. 2003. The avocado fruit borer, Stenoma catenifer (Wals.) (Lepidoptera: Elachistidae): egg and damage distribution and parasitism. Rev. Bras. Frutic. Jaboticabal 25: 432-435.

NAVA, D. E., J. R. P. PARRA, V. A. COSTA, T. M. GUERRA, AND F. L. CONSOLI. 2005. Population dynamics of Stenoma catenifer (Lepidoptera: Elachistidae) and related larval parasitoids in Minas Gerais, Brazil. Florida Entomol. 88: 441-446.

NUNEZ, E. 2008. Plagas de paltos y citricos en Peru, pp. 324-364 In R. Ripa and P Larral [eds.], Manejo de Plagas en Paltos y Citricos. Instituto de Investigaciones Agropecuarias, La Cruz, Region de Valparaiso, Chile.

WOLFENBARGER, D. O., AND B. COLBURN. 1979. The Stenoma catenifer, a serious avocado pest. Proc. Florida State Hort. Soc. 92: 275.

WYSOKI, M., M. A. VAN DEN BERG, G. ISH-AM, S. GAZIT, J. E. PENA, AND G. K. WAITE. 2002. Pests and pollinators of avocado, pp. 223-293 In J. E. Pena, J. L Sharp, and M. Wysoki [eds.], Tropical Fruit Pests and Pollinators, Biology, Economic Importance, Natural Enemies and Control. CABI Publishing, Wallingford, Oxfordshire, U.K.

MARK S. HODDLE (1,2) AND CHRISTINA D. HODDLE (1)

(1) Department of Entomology, University of California, Riverside, CA 92521 (2) Center for Invasive Species Research, University of California, Riverside, CA 92521
TABLE 1. PARTIAL LIFE TABLE FOR STENOMA CATENIFER LARVAE REARED FROM
HASS AVOCADOS COLLECTED FROM A COMMERCIAL ORCHARD IN SAN MIGUEL
DUENAS, SACATEPEQUEZ, GUATEMALA.

 No.
Life Stage of No. Entering Cause of S. catenifer
 S. catenifer Stage Mortality Dying in Stage

Larvae 283 Parasitism:
 Apanteles sp. 63
 Macrocentrus sp. 42

 Unknown Causes 49

Pupae 129 Unknown Causes: 8
 Females
 Males 13

Adults:
 Females 68
 Males 40

Larval to adult survivorship: 38%; Parasitism: 37%; Sex ratio:
59% female.
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Author:Hoddle, Mark S.; Hoddle, Christina D.
Publication:Florida Entomologist
Article Type:Report
Geographic Code:2GUAT
Date:Dec 1, 2008
Words:1499
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