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Arabidopsis unfolded protein response (UPR) using comparative genomics.

ARABIDOPSIS UNFOLDED PROTEIN RESPONSE (UPR) USING COMPARATIVE GENOMICS. KRISTIN S. ROCKETT AND KAROLINA P. MUKHTAR, BIOLOGY DEPT., UNIVERSITY OF ALABAMA AT BIRMINGHAM, BIRMINGHAM, AL 35294.

Endoplasmic reticulum (ER)-mediated protein secretion and quality control has been shown to play an important role in stress responses in both animals and plants. Biotic or abiotic stressors cause massive protein translation that may subsequently lead to misfolding of nascent peptides. This can cause increased amounts of unfolded or malfolded proteins to accumulate in the ER lumen and trigger a signal through transmembrane sensor proteins, such as Ire1, to correct the folding of the peptides. Simultaneously, a signal is sent to nucleus to upregulate production of protein folding components and downregulate other transcription events. Collectively, this cellular response is termed the Unfolded Protein Response (UPR). In this study, a comparative genomics approach was employed to identify Arabidopsis thaliana homologs of yeast genes involved in UPR. 91 T-DNA insertion mutants for upregulated genes and 293 T-DNA mutants for downregulated genes were obtained for this project. Arabidopsis thaliana knock-out lines, containing T-DNA insertions in UPR-related genes have been screened for sensitivity or insensitivity to an abiotic stressor, Tunicamycin, which blocks protein N-glycosylation in ER and leads to acute ER stress. The results show that many of these knock out lines missing various UPR-related proteins have a phenotypic difference when compared to the wild type that is reflected by changes in size and/or leaf pigmentation after Tunicamycin exposure. Our results conclude that the Arabidopsis homologs of yeast UPR-related genes function in plant responses to ER stress.

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Title Annotation:Biological Sciences Poster Abstracts
Publication:Journal of the Alabama Academy of Science
Article Type:Brief article
Geographic Code:1USA
Date:Apr 1, 2014
Words:260
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