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Appropriate Concentration of Acetic Acid for the Preservation of Fresh Cadaveric Brains to be Used in Surgical Training: A Preliminary Study/Concentracion Adecuada de Acido Acetico para la Preservacion de Cerebros de Cadaveres Frescos para Uso en Entrenamiento Quirurgico: Un Estudio Preliminar.


Acetic acid (AA) is a preservative commonly used to prevent bacterial and fungal growth in wound dressings and in the food industry (Febriana et al., 2012; Shukla et al., 2017). It has been used in post-mortem cadavers to preserve the skin color, softness, and joint mobility for fresh cadaveric surgery demonstrations (Brenner, 2014; Varlet et al., 2018). However, using a small amount of AA has been shown to increase contaminations in cadaveric tissue (Trcek et al., 2015), while larger concentrations can lead to the tissue becoming hard, especially in fresh brains.

A tremendous amount of skill and attention to detail is required to adequately address the risks that accompany surgery performed on the nervous system, which means that rigorous training is necessary before a resident can become a competent neurosurgeon. Neurosurgery residency training in Thailand takes at least of 5 years to complete. The Royal College of Surgeons of Thailand (2018) recently declared that certain milestones must be reached before this training is complete, with the ability to perform a number of specific procedures being one of them. One of the many strengths of Khon Kaen University (KKU) neurosurgery residency program is the availability of cadavers, which are donated by local residents through the Department of Anatomy. The Neurosurgery Division of the Faculty of Surgery has been organizing triennial conferences with live instruction and hands-on experience using the heads of these cadavers for the past 5 years for interested residents and neurosurgeons from all over the country. Some of the benefits of practicing on cadavers include the opportunity to familiarize oneself with surgical tools, the ability to become used to the configurations of the cranial vasculature, as well as the chance to feel more comfortable and confident navigating through the complicated skull base. However, there are still problems encountered with using the cadaveric brains that should be addressed. A fixative solution consisting of a mix of glycerin and formalin is routinely used for cadaver preservation at KKU. However, the glycerin makes the brain overly pulpy, rendering the samples impossible to dissect. When preserved with formalin, the brains become hard and inelastic, characteristics unsuitable for dissection. In addition to its distinct, pungent smell and color-altering properties, formalin has been classified as an occupational hazard for health personnel. It has been reported to cause contact dermatitis, upper airway inflammation, asthma, and is implicated in nasopharyngeal cancer, sinonasal cancer, and lymphohematopoietic cancer (Nelson et al., 1986; Raja & Sultana, 2012; Friis et al., 2014; Ponten & Bruze, 2015; Davies, 2016; Kim et al., 2016). Over the past 100 years, various substances have been reported as being used preservation of the cadaveric tissue. The method of preservation mostly involves bathing the whole cadaver in a certain solution, with an unquantifiable portion of said solution entering the brain. Such solutions include fomepizole, phenoxyethanol, formaldehyde, formalin, and diethylene glycol, each of which have several advantages and disadvantages (Brenner).

Acetic acid's use in the preservation of the postmortem cadaver has been documented as early as in the 19th century by French chemist Jean-Nicholas Gannal (Brenner). In particular, glacial acetic acid has been shown to preserve skin color, skin softness, and joint mobility in the cadaveric arm (Tschernezky, 1984). The aim of this study was to investigate the optimal concentration of acetic acid to be poured into the cadaver's cranial cavity in order to give fresh cadaveric brain tissue the most realistic consistency and color.


Six fresh cadaveric heads were obtained from the KKU body donation program, in accordance with the standard procedures of the Khon Kaen University Faculty of Medicine's Department of Anatomy. All of the cadaveric heads were of adults aged more than 18 years and deceased not longer than 3 days, and were stored in a cool preserving chamber. There were no exclusion criteria. Since this was a pilot study, there were yet no standards as to the optimum number of samples. A Burr hole was made at the vertex position in each cadaveric head. The heads were then placed upside down in a 5 [degrees]C refrigerator in order to drain all residual fluids. After 48 h, industrial grade 98.5 % glacial acetic acid was diluted with tap water to 80 %, 60 %, 40 %, 20 %, and 10 % concentrations, and each solution (including one that was undiluted) was poured into a separate head until full. The heads were then placed upright and stored in a 5 [degrees]C refrigerator. After another 48 hours, the level of the acetic acid at the Burr hole was examined, as it tends to decrease, presumably because it flows into and fills up smaller spaces in the skull cavity. Acetic acid at the same concentration was then added fill the head up to the Burr hole entrance. The cadaveric heads were then left in the 5 [degrees]C refrigerator for another 14 days. A traumatic flap was used to access the brain tissue, which was then examined for color and pia mater firmness by ten participants including neurosurgical residents and neurosurgeons. The brain cortex was then dissected with a suction tube and forceps to reveal the underlying brain tissue for inspection. Color, cortical firmness (on a scale of 1-5: 5 = very hard, 4 = fairly hard, 3 = firm, 2 = fairly soft, 1 = very soft), pial stickiness, and participant satisfaction (on a scale of 1-5: 1 = Not at All Satisfied, 2 = Partly Satisfied, 3= Satisfied, 4 = More than Satisfied, 5 =Very Satisfied) were evaluated.


The summary of our findings is shown in Table I. The color of all of the brains was more or less retained, appearing only slightly yellower. The brain that received 20 % acetic acid had a subarachnoid hemorrhage, with the temporal area appearing significantly more pink. All participants agreed that the pial mater of the brain cortex of all samples was firmed and difficult to pry apart. The cadaveric head preserved with 98.5 % acetic had the firmest consistency, with firmness decreasing with the acid concentration (Table I). In addition, it was observed that firmness was not distributed equally in the brains preserved with concentrations lower than 60 % (Table I). Apart from the brain preserved with 40 % solution, all specimens felt firmer near the temporal area. After the pia mater was separated with a scalpel, the brain tissue in all samples was observed to have a liquid-like consistency.


Acetic acid has been used for preservation of the post-mortem cadavers early as in the 19th century (Brenner). However, this study was the first to preliminarily demonstrate the preservative properties and optimal concentrations of acetic acid in fresh human brain tissue for use in skull base surgery training. Although similar properties have been reported in skin and joint preservation (Brenner; Varlet et al.), this study showed that it could also be used to preserve fresh whole brains. There is the possibility that this cheap and accessible solution could be superior to the solutions currently being used as a brain preservation liquid, with all 10 blinded trainees and attending physicians indicating their satisfaction with the results. One weakness of this study was its small sample size. In addition, the fact that one of the brains had a subarachnoid hemorrhage might have affected the amount of acetic acid that was used, compromising the results. Most of the brains tended to have a slightly firmer consistency near the temporal area, presumably due to the pooling of acetic acid on the tentorium cerebelli. However, brains preserved in acetic acid at concentrations of 60 % or higher received higher satisfaction scores, which suggests that acetic acid could have a role in brain preservation for the purpose of skull base surgery training. Further studies with larger samples and tighter control on intracranial pathologies will be performed. In conclusion, acetic acid is shown to be a viable choice for the preservation of brain tissue for the purpose of cadaveric study. The tissue exhibited a desirable amount of firmness and intact color. We recommend using 60 % acetic concentration and above for maximal satisfaction.


We would like to thank the Khon Kaen University Faculty of Medicine Department of Anatomy for providing the valuable cadaveric heads, which were donated by local residents.

PIYAWATTANAMETHA, N.; SOPCHOKCHAI, I.; CHAISIWAMONGKOL, K.; KITKHUANDEE, N.; SIRATARANON, P. & IAMSAARD, S. Concentracion adecuada de acido acetico para la preservacion de cerebros de cadaveres frescos para uso en entrenamiento quirurgico: un estudio preliminar. Int. J. Morphol., 37(3):1101-1110, 2019.

RESUMEN: Se ha demostrado que una pequena cantidad de acido acetico (AA), un preservante comun, aumenta la contaminacion en el tejido del cadaver, mientras que mayores concentraciones pueden endurecer el tejido, particularmente en cerebros frescos. Este estudio intento optimizar la concentracion de AA en la cavidad craneal para producir una consistencia y coloracion cerebral mas cercanos a la realidad. Seis cabezas cadavericas adultas se conservaron con AA glacial descendente en concentraciones de 98,5 %, 80 %, 60 %, 40 %, 20 % y 10 %. Las muestras se mantuvieron a 5 [degrees]C durante 14 dias. Luego se disecco la corteza cerebral con un tubo de succion y pinzas para observar e inspeccionar el tejido cerebral subyacente. Se evaluaron el color, la firmeza cortical, la viscosidad y adherencia de la piamadre y la reaccion de los participantes ante esta conservacion. El color de los cerebros en todas las concentraciones fue ligeramente amarillo. Sin embargo, el area temporal del cerebro, conservada con un 20 % de AA, fue significativamente mas rosada. La piamadre de la corteza de todas las muestras fue de consistencia firme y dificil de separar; una mayor resistencia se observo en el tejido cerebral preservado con 98,5 % de AA. La consistencia del tejido cerebral en todas las muestras era liquida. Los cerebros conservados en AA a una concentracion superior al 60 % recibieron puntuaciones de satisfaccion mas elevadas. Concluimos que el acido acetico desempena un papel en la preservacion del cerebro, permitiendo el entrenamiento en cirugia de base de craneo, por lo que recomendamos concentraciones de AA superiores al 60 % para una satisfaccion maxima por parte de los participantes.

PALABRAS CLAVE: Acido acetico; Concentracion; Conservacion; Cerebro; Cadaver fresco.


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Nontaphon Piyawattanametha (1); Intouch Sopchokchai (1); Kowit Chaisiwamongkol (2); Nawiya Kitkhuandee (3); Punnarat Sirataranon (1); & Sitthichai Iamsaard (2,4)

(1) Neurosurgery Division, Department of Surgery, Khon Kaen Province 40002, Thailand.

(2) Department of Anatomy, Khon Kaen University, Khon Kaen Province 40002, Thailand.

(3) Demonstration School of Khon Kaen University, Khon Kaen Province 40002, Thailand.

(4) Center for Research and Development of Herbal Health Product, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen Province 40002, Thailand.

Corresponding author: Assoc. Prof. Sitthichai lamsaard Department of Anatomy Khon Kaen University Khon Kaen Province 40002 THAILAND


Received: 31-01-2019

Accepted: 30-04-2019

PIYAWATTANAMETHA, N.; SOPCHOKCHAI, I.; CHAISIWAMONGKOL, K.; KITKHUANDEE, N.; SIRATARANON, P. & IAMSAARD, S. Appropriate concentration of acetic acid for the preservation of fresh cadaveric brains to be used in surgical training: A preliminary study. Int. J. Morphol., 37(3):1107-1110, 2019.
Table I. Summary of acetic acid concentrations with satisfaction

Cadaveric heads (H)                     H1        H2        H3

Acetic acid concentration ( %)           98.5 %    80 %      60 %
Initial acetic acid poured (ml)         130        90       100
Additional acetic acid at 48 hr (ml)    130       120       125
Color at 14 days                        Slightly  Slightly  Slightly
                                        yellow    yellow    yellow
Firmness at 14 days (Scales: 1-5)         3         3         3
Pia mater stickiness                    Sticky    Sticky    Sticky
Additional notes                        NA        NA        NA
Participant satisfaction (Scales: 1-5)    5         5          4

Cadaveric heads (H)                     H4        H5

Acetic acid concentration ( %)           40 %     20 %
Initial acetic acid poured (ml)         110       50
Additional acetic acid at 48 hr (ml)    130       80
Color at 14 days                        Slightly  Slightly yellow,
                                        yellow    pink at temporal
Firmness at 14 days (Scales: 1-5)         3        2
Pia mater stickiness                    Sticky    Sticky
Additional notes                        NA        Sylvian
Participant satisfaction (Scales: 1-5)    3        2

Cadaveric heads (H)                     H6

Acetic acid concentration ( %)           10 %
Initial acetic acid poured (ml)         160
Additional acetic acid at 48 hr (ml)    200
Color at 14 days                        Sligh
Firmness at 14 days (Scales: 1-5)         1
Pia mater stickiness                    Stick
Additional notes                        NA
Participant satisfaction (Scales: 1-5)    1

Note: Cortical firmness (5 = Very hard, 4 = fairly hard, 3 = Firm, 2 =
fairly soft, 1 = Very soft). Participant satisfactions (1 = Not at All
Satisfied, 2 = Partly Satisfied, 3= Satisfied, 4 = More than Satisfied,
5 =Very Satisfied).
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Author:Piyawattanametha, Nontaphon; Sopchokchai, Intouch; Chaisiwamongkol, Kowit; Kitkhuandee, Nawiya; Sira
Publication:International Journal of Morphology
Date:Sep 1, 2019
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