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Antifungal and antioxidant activities of the essential oil from Angelica koreana Nakai.

1. Introduction

The roots of Angelica koreana Nakai (Apiaceae) are used in oriental medicine for treatment of cold, especially for patients that present with symptoms such as chills, fever, headaches, body aches, and pains [1]. The acaricidal toxicity and hypopigmenting activity of the components of A. koreana have been reported [2, 3]. Essential oils are one of the most promising sources for the development of new natural antimicrobial and antioxidant agents, even though these oils appear to have relatively mild activities compared to commercial, synthetic agents [4-7].

Aspergillus species cause a number of severe diseases, both in normal and immunocompromised hosts, encompassed under the name aspergillosis and including allergic disease, saprophytic disease, superficial infections, and invasive infections [8-10]. Among the Aspergillus species, A. fumigatus is the most common human infectious agent, followed by A. flavus, A. niger, and A. terreus [11-13]. A. versicolor produces many toxic compounds, which can cause severe symptoms in humans and animals infected through inhalation or other forms of contact with debris or spores [14,15].

Trichophyton species are pathogenic fungi causing superficial mycoses, commonly known as tinea infections, in various tissues of humans and other animals [16]. T rubrum is a predominate cause of dermatophytosis, followed by T. mentagrophytes and T tonsurans [17-20].

Antioxidants are used as food additives to protect humans and food from undesirable oxidative reactions. The antioxidant activities of plant compounds have been increasingly investigated in recent decades for the development of new natural antioxidative agents [21, 22]. Plant essential oils are a promising source of natural antioxidants [23, 24].

Here, to specifically determine whether stable and safe antifungal agents could be developed from the essential oil of Angelica koreana, its inhibiting activity against common pathogenic fungi (three Aspergillus fungi and five Trichophyton species) was investigated using a microbroth dilution test. In addition, we determined the synergistic effects of A. koreana root essential oil fraction and its main components with itraconazole using checkerboard microtiter tests.

To estimate the value of this oil as an antioxidant agent, 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging, nitrite inhibition, and reducing power, the corresponding experiments were performed.

2. Materials and Methods

2.1. Sample Preparation. The essential oil was obtained by steam distillation of the dried roots of Angelica koreana, which was cultivated in Jinbu, Gangwon-do, Korea.

2.2. Fungal Strains. A. flavus KCCM11453, A. flavus KCCM11899, A. flavus KCCM11910, A. fumigatus KCCM60027, A. fumigatus KCCM60331, A. terreus KCCM12067, A. terreus KCCM12709, A. niger KCCM11239, A. niger KCCM11240, A. niger KCCM11241, A. versicolor KCCM12714, A. versicolor KCCM35225, T. tonsurans KCCM11866, T. tonsurans KCCM60442, T mentagrophytes KCCM11950, T mentagrophytes KCCM60444, T rubrum KCCM60443, and T rubrum KCCM60450 were obtained from the Korean Culture Center of Microorganisms (KCCM). They were cultured in yeast and malt extract broth for 48 h at 28[degrees] C. The turbidity of the Aspergillus cell suspensions was measured at 625 nm and adjusted with medium to match the 0.5 McFarland standard. The inocula suspensions of Trichophyton species were prepared using the method as described by the Clinical and Laboratory Standards Institute (CLSI) [25]. The microconidia were counted on a hemocytometer and diluted to a final concentration of ca. 0.5 x [10.sup.4] CFU/mL.

2.3. Gas Chromatography-Mass Spectrometry (GC-MS) Analysis. GC-MS analysis was carried out using a Hewlett Packard (HP) 5973-6890 system equipped with an HP-5MS capillary column. The oven was programmed with an initial temperature of 50[degrees]C and heated at a rate of 2[degrees]C/min to 170[degrees]C.

2.4. Isolation of m-Cresol and Sabinene. The essential oil fraction (10 g) of A. koreana was subjected to column chromatography over a silica gel and was eluted with the solvent, toluene-ethyl acetate (95:5). The fractions were combined based on thin layer chromatography (TLC) pattern in order to yield subfractions designated A1-A10. Subfraction A5 was further purified by column chromatography over a silica gel and was eluted with toluene-ethyl acetate to give 21 mg of m-cresol (99.8%). Sabinene (91.5%) was obtained by repeated fractional distillation with the hydrocarbon fraction of this oil. The ultraviolet spectrometry (UV), mass spectrometry (MS), [sup.1]H-nuclear magnetic resonance spectrometry (NMR), and [sup.13]C-NMR results were identical to those of the authentic standard sample (Sigma, USA).

2.5. Determination of the Minimal Inhibitory Concentrations (MICs). The MICs of the antifungal agents against the various fungi were determined using a modified broth microdilution test as described by the CLSI. The essential oils of Angelica koreana, m-cresol and sabinene, were serially diluted with ethanol to obtain 0.0125 ~ 16 mg/mL solutions, and 10 [micro]L/mL of Tween 80 was added to each solution. After shaking, 100-[micro]L aliquots of these solutions were added to the wells of a 96-well microtiter plate. Itraconazole was similarly diluted in dimethyl sulfoxide (DMSO) to generate a series of concentrations ranging from 128 to 0.25 [micro]g/mL per testing well. A 100-[micro]L suspension of each organism was adjusted to a final concentration of ca. 0.5 x [10.sup.4] CFU/mL and then added to the individual wells and cultivated at 28[degrees]C. The MIC was defined as the lowest concentration that completely inhibited visible fungal growth after 48 h of incubation. Each organism was also cultured with a blank solution containing Tween 80, ethanol, and DMSO at concentrations equivalent to those in the test solutions in order to verify that these vehicles did not affect fungal growth. The tests were performed in triplicate.

2.6. Checkerboard Microtiter Test. Ten serial, two-fold dilutions of A. koreana essential oil, m-cresol, sabinene, and itraconazole were, respectively, prepared with the same solvents used in the MIC tests. Then, 5-[micro]L aliquots of each dilution were added to the wells of a 96-well plate in the vertical orientation, and 5-[micro]L aliquots of each itraconazole dilution were added in the horizontal orientation so that the plate contained various concentration combinations of the two compounds. A 100-[micro]L suspension of each fungal strain (0.4x[10.sup.4] CFU/mL) was added to the individual wells, and the plate was incubated at 28[degrees]C for 72 h. Fractional inhibitory concentrations (FICs) were calculated as the MIC of the combination of essential oil/m-cresol/sabinene and itraconazole divided by the MIC of essential oil/m-cresol/sabinene or itraconazole alone. The FIC index (FICI) was calculated by summing both FICs and was interpreted as a synergistic effect when it was <0.5, as additive when it was 0.5 to 1.0, as indifferent when it was > 1.0 to 2.0, and as antagonistic when it was >2.0 [26].

2.7. DPPH Scavenging Effects of A. koreana Essential Oil. A fresh solution of 0.1 mM DPPH and two-fold dilutions of an A. koreana essential oil fraction (or its main component) (3.20.05 mg/mL, final concentration) were prepared in ethanol, and 900 [micro]L of DPPH was mixed with 100 [micro]L of the oil sample. After vortexing for 10 sec, samples were added to five wells in 96-well plates and kept at room temperature for 30 min. A decrease in absorbance (ABS) was monitored at 540 nm. DPPH radical scavenging capacity was calculated using the following equation [27]:

DPPH scavenging effects (%)

= 100 x [1 - {ABS of sample / (Abs of DPPH - ABS of sample)}] (1)

2.8. Nitric Oxide Scavenging Activity. Nitric oxide scavenging activity was measured using the method of Kato et al. [28]. In brief, 10 [micro]L of sodium nitrite (2mMol) and 10 [micro]L of each serial dilution of the oil sample were mixed with 80 [micro]L of citrate buffer (0.2 Mol) and adjusted to pH = 3.0. The mixtures were incubated at 37[degrees]C for 1h. Next, 200 [micro]L of 2% acetic acid solution was added to 40 [micro]L of each mixture in a 96-well plate. Then, 16 [micro]L of Griess reagent (1% sulfanilamide, 2% phosphoric acid, and 0.1% naphthyl ethylenediamine dihydrochloride) was added to each well. After 15 min, the absorbance of the chromophore formed during the diazotization of the nitrite with sulfanilamide and the subsequent coupling with naphthyl ethylenediamine dihydrochloride was measured at 546 nm [29].

2.9. Determination of Reducing Power. Reducing power was determined according to the method of Elmastas et al. [30]. Various concentrations of A. koreana essential oil fraction were prepared as two-fold dilutions (12.5-200 [micro]g/mL, final concentration) with methyl alcohol. Each sample (0.2 mL) was mixed with 0.6 mL of 0.2 M phosphate buffer (pH 6.6) and 0.6 mL of 1% potassium ferricyanide. The mixture was incubated at 50[degrees]C for 20 min, and 0.6 mL of 10% trichloroacetic acid was added before 10 min of centrifugation at 1000 xg (Hanil Science, Korea). The upper layer (1mL) was mixed with Fe[Cl.sub.3] (0.1 mL, 0.1%), and its absorbance was measured at 700 nm. Higher absorbance indicated greater reductive capability.

2.10. Single Dose Oral Toxicity Study. This study was carried out to evaluate the single dose oral toxicity of the volatile oil fraction of A. koreana in male specific-pathogen-free (SPF; Central Lab Animal Inc, Seoul, Korea) ICR mice. The test substance was administered to male and female mice at doses of 500,1,000, and 2000 mg/kg. A group of five mice of each gender (5 animals per group) was treated with each dose. Mortalities, clinical signs, and body weight changes were monitored for 7 days compared to the vehicle control group. At the end of the 7-day observation period, all animals were sacrificed according to the animal experiment guidelines of the Korean Food and Drug Administration, and necropsy findings were noted.

3. Results and Discussion

The essential oil fraction was obtained by repeated steam distillation of the dried roots of A. koreana. The content of essential oil in A. koreana roots was 0.28%. The composition of the essential oil fraction was analyzed by GC-MS. As listed in Table 1, 48 compounds were identified in this oil, which account for 98.72% of the total composition. Sabinene (31.85%) was the predominant component ofthis oil, followed by m-cresol (4.46%) and [alpha]-pinene (4.00%), [alpha]-bisabolol (3.63%) and [alpha]-bornyl acetate.

The MICs of the essential oil fraction, its main components, sabinene and m-cresol, and the antibiotics amphotericin B and itraconazole are presented in Table 2. In the three samples of the oil, m-cresol showed the strongest growth inhibition against most of the tested fungi, with minimum inhibitory concentrations (MICs) between 125 and 1000 [micro]g/mL. Sabinene exhibited the lowest activity, with MICs ranging from 500 to >4000 [micro]g/mL. In most of the experiments, the Trichophyton species showed relatively higher sensitivity to the tested Angelica koreana oil fraction and its two main components compared to the Aspergillus species. The oil fraction showed the highest potency against T. mentagrophytes, with MICs between 250 and 500 [micro]g/mL. However, the MICs of the oil were much higher than those of the antibiotics amphotericin B and itraconazole (MICs = 0.25-8.00 [micro]g/mL), which were used as controls. In most cases, the antibiotic activities of plant essential oils are weaker than those of synthetic antibiotics used commonly in therapy. For this reason, their application in the therapy of fungal infections is very limited. Nevertheless, in consideration of the side effects of the antibiotics, essential oils have been considered as a source for the development of new natural antibiotics. Amphotericin B is highly toxic in its conventional form and expensive in its lipidic form [31]. Itraconazole is a relatively well-tolerated drug, but it has relatively low bioavailability after oral administration and produces a range of adverse effects similar to those of other azole antifungals [26]. Checkerboard titer tests were performed in this study in order to determine the synergism created by the combination of these two groups of compounds with regard to overcoming the weak activity of the oil and to minimize the side effects and toxicity of antibiotics by lowering the dosage.

The FICs and FICIs results from these checkerboard tests are listed in Tables 3 and 4. There was significant synergism and additive effects between itraconazole and A. koreana oil fraction/m-cresol, with FICI values ranging from 0.26 to 1.00 against the Aspergillus and Trichophyton species. Among the tested fungi in this study, A. fumigatus and A. terreus showed the most distinctive synergism with the combination of A. koreana oil fraction with itraconazole, resulting in an FICI of 0.37 for both Aspergillus species. The FICIs between 0.65 and 1.00 in the tests with Trichophyton species indicated additive effects between itraconazole and A. koreana oil fraction/m-cresol. In particular, this combination against T. rubrum resulted in an eight-fold decrease in the MIC with itraconazole (FIC = 0.12) compared to that before combination with the essential oil fraction. Thus, A. koreana essential oil might be useful in antifungal therapy when combined with itraconazole, especially against Aspergillus and Trichophyton species.

To evaluate A. koreana oil as an antioxidant, DPPH free radical scavenging activity, nitrite formation inhibition, and reducing power procedures were performed with the oil fraction and its two main components using butylated hydroxyl anisole (BHA, Sigma-Aldrich, USA).

As demonstrated in Figure 1, the essential oil of A. koreana and its main components showed significant dose-dependent DPPH scavenging activities. One of the main components of the Angelicaessential oil fraction, m-cresol (56.12 [+ or -] 1.36%), showed greater inhibition of DPPH than did the oil fraction or sabinene (19.31 [+ or -] 1.02% and 4.45 [+ or -] 1.06%, resp.) at concentrations of 16mg/mL. However, the results showed weaker scavenging activity than the control. At 1 mg/mL, the activity of BHA was more than 20 times higher than those of all three of the tested oil samples.

In nitric oxide scavenging tests, the Angelica oil fraction and its main components, m-cresol and sabinene, showed significant scavenging activities dose-dependently (Figure 2). Sabinene, exhibited the higher activity than the oil fraction or m-cresol.

The results of reducing power assays are demonstrated in Figure 3. The A. koreana essential oil exhibited significant activity at concentrations of 0.25-4.00 mg/mL. Sabinene showed the strongest reducing power at 2.00 and 4.00 mg/mL. At 4.00 mg/mL, both the oil and sabinene resulted in high absorbance (0.90 [+ or -] 0.09 and 1.16 [+ or -] 1.06, resp.), indicating strong reducing power, while m-cresol showed relatively weaker activity. The absorbance tested with BHA was 1.23 [+ or -] 0.00 at the concentration of 0.25 mg/mL. This indicates that the concentration of Angelica oil needs to be five times greater than that of BHA in order to produce the same reducing power of BHA. As these results indicated, the antioxidant activity of the A. koreana essential oil appeared weaker than that of BHA, although its use would avoid the toxicity issues of BHA [32].

In the single dose oral toxicity study with male SPFICR mice, there was no death of any animal in the group administered 500 mg/kg of essential oil fraction during the experimental period. No clinical signs or body weight changes were found in relation to the tested samples. The LD50s of the sample were 1437.5 mg/kg for male mice. On the basis of the LD50 and MICs, toxicity could be excluded in the use of A. koreana essential oil as an anti-Aspergillus and anti-Trichophyton agent, especially in combinatorial therapy with antibiotics.

4. Conclusion

For the treatment of infections caused by Aspergillus and Trichophyton species, combining itraconazole with either A. koreana essential oil or its main components, sabinene and m-cresol, may reduce the minimum effective dose of itraconazole required and thus minimize the side effects. In addition, A. koreana essential oil is a promising source of new natural antioxidants. However, further studies are required to assess the potential for clinical application.

Conflict of Interests

The authors declare that they have no conflict of interests.


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Junghyun Roh and Seungwon Shin

College of Pharmacy, Duksung Women's University, Seoul 132-714, Republic of Korea

Correspondence should be addressed to Seungwon Shin;

Received 29 May 2014; Revised 16 July 2014; Accepted 20 July 2014; Published 12 August 2014

Academic Editor: Yitao Wang

Table 1: Constituents of essential oil from A. koreana according
to GC-MS.

Compounds                   RI (a)    Area (%)    Identified
                                     A. koreana       by

[alpha]-Pinene               905        4.00       MSb, GCc
Camphene                     924        0.16          MS
[beta]-Pinene                961        0.31        MS, GC
[apha]-Phellandrene          1004       0.75          MS
[delta]-3-Carene             1005       2.50          MS
Sabinene                     1025      31.85          MS
[beta]-Thujene               1031       0.11          MS
[alpha]-Terpinolene          1085       0.92        MS, GC
m-Cresol                     1098       4.46        MS, GC
Isovaleric acid              1107       1.61          MS
p-Cresol                     1163       0.14        MS, GC
[beta]-Pinone                1186       1.66          MS
[alpha]-Bornyl acetate       1282       2.30        MS, GC
4-Hydroxy-3-methoxy          1290       0.54          MS
Thymol                       1290       0.21        MS, GC
1-Pentalenone                1299       1.84          MS
Isoledene                    1353       0.34          MS
Valencene                    1370       0.20          MS
[delta]-Cadinene             1379       0.42          MS
Cedrene                      1398       0.27          MS
[alpha]-Farnesene            1428       0.48          MS
[beta]-Cubebene              1455       0.85          MS
[beta]-Cedrene               1466       1.01          MS
Thujopsene                   1496       0.47          MS
[alpha]-Muurolene            1503       0.12          MS
Sativene                     1525       0.36          MS
[beta]-Sesquiphellandrene    1530       0.91          MS
Elemol                       1531       0.52          MS
Guaiacyl ethane              1554       1.94          MS
Viridiflorol                 1580       0.62          MS
Anise alcohol                1581       0.20          MS
[alpha]-Amorphene            1594       0.34          MS
1,4-Methanoazulene           1618       0.85          MS
Aromadendrene                1649       0.54          MS
[beta]-Eudesmol              1654       1.15          MS
[alpha]-Eudesmol             1659       1.14          MS
Isocomene                    1673       0.41          MS
Spirodec-6-en-8-one          1688       0.61          MS
a-Bisabolol                  1699       3.63          MS
Z-9-Pentadecenol             1734       1.34          MS
4-Butenylcyclohexene         1743       0.26          MS
Cyclohexene                  1767       0.21          MS
Aromadendrene oxide          1777       0.22          MS
Cedrenol                     1782       0.26          MS
Widdrol                      1902       0.15          MS
3-hydroxy-4-dimethyl         1907       0.15          MS
[delta]-3-Carene             1963       0.29          MS
Osthole                      2159       2.27        MS, GC

Total composition                      98.72

RI (a): retention indices calculated against C9 to
C24 n-alkanes on an HP-5MS column.

MS (b): mass spectrum, GCc: Co-GC with a corresponding
standard compound.

Table 2: MICs ([micro]g/mL) of the essential oil of
A. koreana against Aspergillus and Trichophyton species.

Fungal strains                  EOAK (a)   Sabinene   m-Cresol

A. flavus KCCM11453               1000       2000       500
A. flavus KCCM11899               2000       4000       1000
A. flavus KCCM11910               2000       4000       1000
A. fumigatus KCCM60027            2000       1000       1000
A. fumigatus KCCM60331            2000       1000       1000
A. niger KCCM11239                2000      >4000       1000
A. niger KCCM11240                2000      >4000       1000
A. niger KCCM11241                1000      >4000       1000
A. terreus KCCM12067              1000       4000       500
A. terreus KCCM12709              1000       4000       500
A. versicolor KCCM12714           250        4000       250
A. versicolor KCCM 35225          250        4000       250
T. mentagrophytes KCCM11950       250        500        250
T. mentagrophytes KCCM60446       250        500        125
T. rubrum KCCM60443               500        500        250
T. rubrum KCCM60450               500        500        250
T. tonsurans KCCM11866            500        1000       250
T. tonsurans KCCM60442            250        500        125

Fungal strains                  Amp B (b)   Itraconazole

A. flavus KCCM11453                 2            1
A. flavus KCCM11899                 2            1
A. flavus KCCM11910                 4            2
A. fumigatus KCCM60027              8            2
A. fumigatus KCCM60331              8            2
A. niger KCCM11239                  8            2
A. niger KCCM11240                  4            2
A. niger KCCM11241                  8            2
A. terreus KCCM12067               32           0.25
A. terreus KCCM12709               16           0.25
A. versicolor KCCM12714             4            1
A. versicolor KCCM 35225            4            1
T. mentagrophytes KCCM11950        NT (c)        4
T. mentagrophytes KCCM60446        NT            4
T. rubrum KCCM60443                NT            8
T. rubrum KCCM60450                NT            4
T. tonsurans KCCM11866             NT            8
T. tonsurans KCCM60442             NT            8

EOAK (b): essential oil fraction from the roots of
A. koreana, Amp B (b): amphotericin B, NT (c): : not tested.

Table 3: Fractional inhibitory concentrations (FICs) and FIC
indices (FICIs) of A. koreana essential oil in combination
with itraconazole against Aspergillus spp.

                A. flavus         A. fumigaus
                KCCM11899         KCCM 60027

Sample          FIC      FICI     FIC      FICI

Essential oil   0.50              0.25
fraction                 1.00              0.37
Itraconazole    0.50              0.12

m-Cresol        0.25     0.50     0.25     0.50
Itraconazole    0.25              0.25

                A. niger          A. terreus
                KCCM11241         KCCM12067

Sample          FIC      FICI     FIC      FICI

Essential oil   0.50              0.25
fraction                 0.56     0.25     0.37
Itraconazole    0.06              0.12

m-Cresol        0.25     0.26     0.50     0.62
Itraconazole    0.06              0.12

                A. versicolor
                KCCM 35225

Sample          FIC      FICI

Essential oil   0.25
fraction                 0.50
Itraconazole    0.25

m-Cresol        0.06     0.56
Itraconazole    0.50

FIC (fractional inhibitory concentration) = MIC tested
in combination/MIC tested with single sample alone.

FICI = FIC of A. koreana essential oil or m-cresol
oil component + FIC of itraconazole.

Table 4: Fractional inhibitory concentrations (FICs) and FIC indices
(FICIs) of A. koreana essential oils in combination with
itraconazole against Trichophyton spp.

Sample                   T. mentagrophytes   T rubrum
                         KCCM11866           KCCM11950

                         FIC      FICI       FIC      FICI

Essential oil fraction   0.50     1.00       0.50     0.65
Itraconazole             0.50                0.12

m-Cresol                 0.50     1.00       0.25     0.75
Itraconazole             0.50                0.50

Sample                   T. tonsurans
                         KCCM 60443

                         FIC      FICI

Essential oil fraction   0.50     1.00
Itraconazole             0.50

m-Cresol                 0.50     1.00
Itraconazole             0.50

FIC (fractional inhibitory concentration) = MIC tested
in combination/MIC tested with single sample alone.

FICI = FIC of A. koreana essential oil or m-cresol +
FIC of itraconazole.
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Title Annotation:Research Article
Author:Roh, Junghyun; Shin, Seungwon
Publication:Evidence - Based Complementary and Alternative Medicine
Article Type:Clinical report
Date:Jan 1, 2014
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