Antibacterial, Anti-Inflammatory, Antioxidant, and Antiproliferative Properties of Essential Oils from Hairy and Normal Roots of Leonurus sibiricus L. and Their Chemical Composition.
For millennia, plants have held great economic value, not only by playing a significant role in the food supply but also by acting as therapeutic agents . Interest has recently grown in the application of plants as medicinal agents since several side effects have been found to be associated with synthetic drugs. Plants are known to have therapeutic ability and contribute to reducing the risk of various inflammatory conditions and cancers and are the most important global source of drugs; about 50 percent of drugs produced in the world are of natural origin . According to the World Health Organization, about 80% of people rely on traditional remedies such as herbal drugs, which are found in many modern medicinal formulations. Many naturally occurring agents in plant extracts or oils have shown antimicrobial, antioxidant, anticancer, and anti-inflammatory potential in several animal models and bioassay systems and have an effect on human disease [3-6].
One particularly interesting group of compounds are the essential oils obtained from various parts of plants. Although essential oils only represent a small fraction of plant's composition, they nevertheless confer the characteristics by which aromatic plants are used in the food, cosmetic, and pharmaceutical industries . The antioxidant activity of essential oils is another biological property of great interest because they may preserve foods from the toxic effects of oxidants . Moreover, their free radical scavenging ability may play an important role in some diseases' prevention such as brain dysfunction, cancer, heart disease, and immune system decline [9,10].
One plant which shows a broad spectrum of biological activity is Leonurus sibiricus L. Modern pharmacological studies have shown that the active components in various parts of Leonurus sibiricus possess a wide range of pharmacological activities, with effects on the uterus, as well as bestowing more general cardioprotective, antioxidative, anticancer, analgesic, anti-inflammatory, neuroprotective, and antibacterial effects [11-14]. Earlier studies have demonstrated the anticancer and antioxidant activities of various parts of this plant . The most interesting results for extract of Leonurus sibiricus were obtained for in vitro culture transformed and normal roots . Literature data shows that transformation of the roots can encourage the production of valuable secondary metabolites . Therefore, the present study was carried out to evaluate the antimicrobial, anti-inflammatory, antioxidant, and antiproliferative activities and identify the chemical profile of essential oils from L. sibiricus normal and hairy roots.
2. Materials and Methods
2.1. Plant Material of L. sibiricus Roots. In this study, normal (NR) and hairy roots (HR) of L. sibiricus were tested. The hairy roots were obtained by infection of five-week-old in vitro shoots of L. sibiricus with Agrobacterium rhizogenes strain A4. Establishment of hairy root (HR) cultures has been described previously by Sitarek et al. .
2.2. Isolation and Analysis of Essential Oils. The essential oils of NR and HR roots (about 50 g of each) were obtained by hydrodistillation, using a clevenger-type apparatus for 5h. Chemical analysis of essential oils composition was performed by GC-MS method according to Makowczynska et al. . Apparatus details are as follows: Trace GC Ultra with FID and MS DSQ II detector and MS-FID splitter, with column Rtx-1ms (Restek), 60 m x 0.25 mm i.d., and film thickness 0.25 [micro]m; temperature program, 50-310[degrees]C at 2[degrees]C/min; injector temp. 280[degrees]C; FID temp. 300[degrees]C; carrier gas helium and ionization voltage 70 eV; ion source temp. 200[degrees]C. The identification of the compounds was based on the comparison of their RIs and MS spectra with those stored in the computer libraries and literature data [19, 20]. The percentages were computed from FID response without the use of correction factors. The results are showed in Table 1.
2.3. Cell Culture. Normal human astrocytes (NHA) were grown in AGM medium according to the manufacturer's protocol. The cells were seeded at a density of 4 x [10.sup.5] cells per 25 [cm.sup.2] flask. Under these conditions, the cell cycle of this line was approximately 12 hours. Glioma cells in IV grade were derived from tumour patient. Condition and further procedures are described in a previous study by Sitarek et al. .
2.4. MTT Assay. Cell viability was determined by MTT assay. Briefly, NHA (normal human astrocytes) and glioma cells in IV grade (4x[10.sup.5] cells) were cultured in four-well plates for 24 hours after treatment by each concentration (0-3200 [micro]g/mL) of HR and NR essential oils of L. sibiricus root. Further procedures are described in a previous study by Sitarek et al. .
2.5. Antimicrobial Properties
2.5.1. Microbial Strains and Growth Conditions. The bacterial strains Enterococcus faecalis (ATCC29212), Staphylococcus aureus (ATCC 25923), Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 25922 were incubated at 37[degrees] C in Mueller-Hinton medium (Biokar Diagnostics, Beauvais, France). The yeasts Saccharomyces cerevisiae ATCC 2601 and Candida albicans ATCC 10231 were grown in Sabouraud Dextrose Agar (Difco).
2.5.2. Microdilution Method. The essential oil minimum inhibitory concentrations (MICs) were determined using the twofold serial broth microdilution assay . For bacteria, the samples dissolved in DMSO were diluted in the range 500 to 0.48 [micro]g/mL in Mueller-Hinton broth medium, while, for the yeasts, the samples were diluted in the range 5000 to 4.88 [micro]g/mL in Sabouraud medium. The antimicrobial activity of DMSO was evaluated. Vancomycin, norfloxacin, and amphotericin B were used as controls. The lowest concentrations of the samples that inhibited the growth of the microorganisms after 24 hours of incubation at 37[degrees] C were adopted as the MIC values; these are presented in [micro]g/mL. Microbial growth was measured at 620 nm using an Absorbance Microplate Reader (Thermo scientific Multiskan FC). Assays were carried out in triplicate for each tested microorganism.
2.5.3. Minimum Bactericidal Concentration (MBC) or Minimum Fungicidal Concentration (MFC). Following the MIC determination, the MBC or MFC was found for each set of wells. A loopful of broth was collected from the wells which did not show any growth, and this was inoculated on sterile Mueller-Hilton medium broth for bacteria and Sabouraud medium for yeasts by streaking; these media were then incubated at 37[degrees]C for 24 hours. After incubation, the lowest concentration at which no visible growth was observed was recorded as MBC (for bacteria) or MFC (for yeasts).
2.6. Anti-Inflammatory Activity. Levels of IL-1[beta], IL-2, IL-4, IL-6, IFN-[gamma], and TNF-[alpha] in supernatant were measured by ELISA. The astrocytes cells (NHA) were subcultured in 6-well plates (5 x [10.sup.5] cells/mL) and incubated with NR and HR essential oil (15, 30, and 60 [micro]g/mL) or DMSO (vehicle) for 24 hours and incubated with LPS (1 [micro]g/mL) for 24 h. After treatments, cell-free supernatant was collected, and IL-1[beta], IL-2, IL-4, IL-6, IFN-[gamma], and TNF-[alpha] were measured using ELISA kits, in accordance with the manufacturer's instructions (Qiagen, USA). The absorbance was determined using a microplate reader. Results represent the mean [+ or -] SD of three independent experiments.
2.7. Reverse Transcriptase-PCR Analysis. Total RNA was isolated from astrocyte cells according to the manufacturer's instructions using a Total RNA Isolation System (Blirt, Poland). cDNA was generated by Reverse Transcription System (A&A Biotechnology, Poland), and 10 [micro]L of the cDNA product was used as a template in the PCR reaction. Briefly, the PCR amplification consisted of 35 cycles (94[degrees]C, 1min; 55-65[degrees]C, 1min; 72[degrees]C, 2 min) with the commercial oligonucleotide primer sets for human IL-1[beta], IL-2, IL-4, IL6, IFN-[gamma], and TNF-[alpha]. These primer sets yield PCR products of 93, 262, 74, 296, 75, and 406 bp for IL-1[beta], IL-2, IL-4, IL-6, IFN-[gamma], and TNF-[alpha], respectively. The final PCR products were electrophoresed on 1.5% agarose gels. Further procedures are described in a previous study by Sitarek et al. .
2.8. Antioxidant Activity
2.8.1. Evaluation of DPPH Radical Scavenging Assay. DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity was assessed according to Blois  with some modifications. Briefly, a series of dilutions of NR and HR essential oils were made. Following this, 1.5 mL of each concentration was mixed with 1.5 mL of a 0.2 mmol/L methanolic DPPH solution. After a 30-minute incubation period at 25 C, the absorbance at 520 nm (maximum absorbance of DPPH) was recorded spectrophotometrically as [A.sub.(sample)]. A blank reading was taken using a cuvette containing solution without the test material, and the absorbance was measured as [A.sub.(blank)]. The free radical scavenging activity of each solution was then calculated as percentage inhibition according to the following equation:
% inhibition = 100([A.sub.(blank)] - [A.sub.(sample)])/ [A.sub.(blank)]
The antioxidant activity of the essential oil was expressed as [EC.sub.50], defined as the concentration of the test material required to cause a 50% decrease in initial DPPH concentration. BHA was used as a standard. All measurements were performed in triplicate.
2.8.2. ABTS Radical Cation Scavenging Assay. The capacity of the samples to scavenge the ABTS (2,20-azinobis-3-ethylbenzothiazoline-6- sulphonate) radical cation was determined according to Re et al.  with some modifications. The ABTS radical cations were generated by mixing a 7 mmol/L of ABTS at pH 7.4 (5 mmol/L Na[H.sub.2]P[O.sub.4], 5 mmol/L [Na.sub.2]HP[O.sub.4], and 154 mmol/L NaCl) with 2.5 mmol/L potassium persulfate (final concentration). The mixtures were then stored in the dark at room temperature for 16 hours. Following this, the mixture was diluted with water to give [A.sub.734] value of 0.69 [+ or -] 0.03 units using a spectrophotometer. For the sample, solutions of the essential oil in methanol were allowed to react with fresh ABTS solution; the absorbance was then measured six minutes after the initial mixing. BHA was used as a standard. The free radical scavenging capacity was expressed as [EC.sub.50] ([micro]g/mL), which was determined using the same equation used for the DPPH method. All measurements were performed in triplicate.
2.9. Statistical Analysis. The results are presented as the mean [+ or -] standard deviation of at least three separate experiments. Statistical differences were determined by one-way ANOVA. Results with P < 0.05 were considered statistically significant.
3.1. Chemical Composition of NR and HR of Essential Oils from L. sibiricus Roots. In this study we demonstrated qualitative and quantitative essential oil compositions from NR and HR of L. sibiricus. Essential oils from NR and HR were obtained with yields of 0.06% and 0.04%, respectively. Eighty-five components of NR and HR root essential oils were identified (Table 1). NR and HR essential oils contained a complex mixture consisting mainly of sesquiterpene hydrocarbons (60.2% and 64.8%, resp.), oxygenated sesquiterpenes (6.8% and 13.4%, resp.), and monoterpene hydrocarbons (6.0% and 1.5%, resp.). We had a little portion of oxygenated monoterpenes (2.1% and 1.9%) (Table 1). For NR essential oil, seventy components were identified. The major constituents in NR essential oil were [beta]-selinene (9.9%), selina-4,7-diene (9.7%), (E)-[beta]-caryophyllene (7.3%), myli-4(15)-ene (6.4%), and guaia-1(10),11-diene (5.9%). In HR essential root oil, sixty-seven components were identified. The main constituents of HR essential oil were (E)-[beta]-caryophyllene (22.6%), germacrene D (19.8%), and [alpha]-copaene (5%).
3.2. MTT Viability on Normal Human Astrocytes and Glioma Cells after Treatment with Essential Oils ofL. sibiricus NR and HR. First, to test the viability of cells after treatment with LPS, NR, and HR essential oils of L. sibiricus roots, MTT assay was performed in NHA and glioma in IV grade cells (Figures 1(a) and 1(b)). LPS did not affect cell viability in tested concentration (1 [micro]g/mL) after 24 h (data not shown). Our results showed that viability was similar for both essential oils. The [IC.sub.50] value was considered as the concentration of 1600 [micro]g/mL for NR and HR essential oils which caused a 50% decrease in cell viability relative to the negative control, comprising cell culture and DMSO without either of the essential oils (Figure 1(a)). Additionally, the NR and TR oils were found to have a cytotoxic effect on IV grade glioma cells at the IC50 concentration of 400 [micro]g/mL. The tested NR and HR root essential oils of L. sibiricus did not affect viability of the NHA cells in [IC.sub.50] concentration for IV grade of glioma cells (Figure 1(b)).
3.3. Antimicrobial Activity. The antimicrobial activities of the essential oils from NR and HR of L. sibiricus root were quantitatively assessed by determination of the MIC and MBC/MFC, as given in Table 2. MIC and MBC/MFC values of oils from NR and HR of L. sibiricus roots were in the range of 125 to 2500 [micro]g/mL for MICs and 500 to 5000 [micro]g/mL for MBC/MFCs for all tested strains. Antibacterial activity was shown by NR and HR oils of L. sibiricus roots against all bacterial strains with MICs values in the range of 125-250 [micro]g/mL, but the highest value was observed against P. aeruginosa (125 [micro]g/mL). L. sibiricus oils were found to have substantial antifungal activity against S. cerevisiae and C. albicans with MIC values of 1250 and 2500 [micro]g/mL, according to microdilution assay, and for MFC values of 2500 and 5000 [micro]g/mL (Table 2). No significant differences were observed between the NR and HR essential oils of L. sibiricus roots.
3.4. Antioxidant Activity. The antioxidant activities of NR and HR essential oils of L. sibiricus root were assessed by free radicals (DPPH and ABTS) scavenging assays. No significant differences were observed between NR and HR essential oils.
In the ABTS assay, [EC.sub.50] values were determined to be 92.40 [+ or -] 1.99 [micro]g/mL for Nr oil and 88.22 [+ or -] 1.35 [micro]g/mL for HR oil, respectively. In turn, [EC.sub.50] values were 500.50 [+ or -] 1.37 [micro]g/mL for NR oil and 489.20 [+ or -] 1.45 [micro]g/mL for HR oil in DPpH assay. Both tested oils showed similar antioxidant effectiveness but better activity was observed for the ABTS method. The results were presented in Table 3.
3.5. Effects of HR and NR Essential Oils from L. sibiricus Roots on IL-1[beta], IL-2, IL-4, IL-6, IFN-[gamma], and TNF-[alpha] Level. The study examined the influence of NR and HR essential oils of L. sibiricus roots on the LPS-stimulated production of IL-1[beta], IL-2, IL-4, IL-6, IFN-[gamma], and TNF-[alpha] in NHA cells. Our results showed that NR and HR root oils decreased level of LPS-stimulated IL-1[beta], IL-6, TNF-[alpha], and IFN-[gamma] compared to controls (Figure 2). This effect was observed at various concentrations (15,30, and 60 [micro]g/mL) of NR and HR essential oils and was dependent on the dose. No differences in IL-2 and IL-4 level were observed with regard to controls for NR or HR essential oils, and no significant differences were observed between the two essential oils.
3.6. Effects of NR and HR Root Oils of L. sibiricus IL-1[beta], IL-2, IL-4, IL-6, IFN-[gamma], and TNF-[alpha] on mRNA Expression. Based on the previous results, the optimal concentrations for both essential oils were selected for RT-PCR analysis to determine whether NR and HR oils of L. sibiricus root can regulate IL1[beta], IL-2, IL-4, IL-6, IFN-[gamma], and TNF-[alpha] mRNA expression. NHA cells were incubated with LPS and then in the presence of 60 [micro]g/mL NR and HR root oils. As shown in Figure 3, the expression of IL-1[beta], IL-6, IFN-[gamma], and TNF-[alpha] genes was found to be lower than in LPS-stimulated cells for both essential oils. No changes in expression were found for IL-2 or IL-4.
Natural plant products have been used since ancient times, and their use is now increasing. Some essential oils are known to have various health benefit properties and have been used as alternative remedies for the prevention and treatment of many infectious diseases: their antibacterial, antifungal, anti-inflammatory, anticancer, and antioxidative activities make them particularly suitable for this role. It is known that the efficacy of the essential oil depends on many environmental and genetic factors. It has been proven that the main factors responsible for the diverse chemical composition of essential oils include climatic conditions, geographic origin, time of collection, distillation conditions, correct farming practices, and the part of the plant from which oil is extracted [24,25]. An important characteristic of essential oils and their components is their hydrophobicity, which enables them to partition the lipids of the bacterial cell membrane and mitochondria, disturbing the structures and rendering them more permeable, resulting in the leakage of ions and other cell contents . The antimicrobial, anticancer, anti-inflammatory, and antioxidative properties of essential oils have been evaluated by many other researchers [27, 28] and their results support the findings of the present investigation. The present study investigated the chemical profile of essential oils derived from the normal and hairy roots of Leonurus sibiricus and evaluated their antimicrobial, anti-inflammatory, antioxidant, and antiproliferative activities.
The essential oils were isolated from the L. sibiricus herb or its leaves according to a previous study . This study is the first to identify the essential oil compositions of L. sibiricus normal and hairy roots. Dambolena et al.  report that [beta]-caryophyllene and a-humulene were two of three main constituents, amounting in total to 75-80% in both oils (leaves and herb), with [beta]-cubebene as the third. In turn, Almeida et al. found germacrene D to be the most dominant compound in leaf oil. In other species of the genus Leonurus, L. japonicus sesquiterpenes (45.4%) with [beta]-caryophyllene and its oxide are the main constituents of essential oil isolated from the herb .
In the present study, [beta]-caryophyllene (22.6% of total) and germacrene D (19.8%) were found to be the main constituents in HR essential oil, being present in higher concentrations in HR than in NR (7.3%, 1.2%, resp.) essential oil. Additionally, the analysis of NR essential oil revealed other compounds including selina-4,7-diene, selina-4,11-diene, and guaia-1(10),11-diene which were not detected in HR essential oil. The other main components in NR essential oil were [beta]-selinene and myli-4(15)-ene. In addition, [beta]-selinene was present in ninefold higher concentrations, and myli-4(15)-ene sixfold higher, in NR essential oil than in HR oil. Despite the different contents, no significant differences were found regarding biological activities.
Although many workers have examined the antioxidant activity of essential oils by DPPH and ABTS assay on a range of species [31, 32], none have discussed the antioxidant activity of essential oil from NR and HR of L. sibiricus root. Our studies showed both oils to have the low reactivity to DPPH assay ([EC.sub.50] = 5 0 0 [micro]g/mL and [EC.sub.50] = 489 [micro]g/mL) and moderate reactivity to ABTS assay ([EC.sub.50] = 92 [micro]g/mL and [EC.sub.50] = 88 [micro]g/mL), which may be associated with the high concentrations of sesquiterpene hydrocarbons in NR and TR essential oils (60.2% and 64.8%, resp.). Our findings are consistent with those of Andrade et al., which indicate that the low antioxidant activity of essential oils of Siparuna guianensis results from the high content (59.4%) of sesquiterpene and monoterpene hydrocarbons . The DPPH test is based on the use of a very crowded radical; however, the use of a low crowded radical such as ABTS can be more suitable : the ABTS assay gives better results for expressing antioxidant property because it is sensitive, requires a short reaction time, and can be used in both organic and aqueous solvent systems . The difference between the results of the DPPH and ABTS tests may be attributed to the mechanism of the involved reaction: the reactions of the ABTS radical involve electron transfer and take place at a much faster rate compared to DPPH radicals, whose amount of discoloration is attributed to the hydrogen donating ability of the test compounds [31, 32].
The essential oils showed a pronounced effect against grade IV glioma cell line, with [IC.sub.50]values of 400 [micro]g/mL. A novel aspect of our findings is that they demonstrate that NR and HR essential oils from L. sibiricus roots have cytotoxic effects. Legault and Pichette showed that [beta]-caryophyllene has an anticancer activity against the cell line MCF-7 . This compound was identified in the HR and NR essential oils. In turn, de Oliveira et al. found the oils to have anticancer effects in various cancer cell lines, with germacrene D as one of the main compounds in the essential oils . However, previous studies indicate that the anticancer activity of the essential oil might be due to the synergic effects of all the terpenes in the oil; alternatively there may be some other active compounds responsible for the anticancer activity of the essential oil, which deserves attention in future study. The MTT assay found that the NR and HR essential oils from L. sibiricus root used in the present study did not affect the viability of NHA cells when applied in the concentration range 0-800 [micro]g/mL.
The essential oils of many plants are known to have antimicrobial activity [4, 36, 37], which could act as a chemical defence against various pathogens. The present study examines the antimicrobial activities against all tested strains, which may be attributed to the presence of sesquiterpenes such as (E)-[beta]-caryophyllene or germacrene D in the tested oils. It is known that [beta]-caryophyllene and germacrene D possess strong antimicrobial activity . Dahham et al. showed that [beta]-caryophyllene has selective antibacterial activity and more pronounced antifungal activity . Xiong et al. note that the oil of Leonurus japonicus possesses antimicrobial activity against Gram-positive bacteria but not against Gram-negative bacteria . In contrast, our results showed antimicrobial activity against Gram-positive and Gram-negative bacteria. If the MBC of a pure compound is no more than four times the MIC value, it can be regarded as bactericidal; hence these essential oils appear to offer potential as a bactericide . However, it is difficult to compare our data with other studies because the variables that influence the results are the essential oil composition and antimicrobial test method used. Moreover, the standard criteria for the evaluation of the activity of the plant essential oils are missing and therefore the results obtained by different authors vary widely. Due to the high antimicrobial activity of essential oils, they may have valuable use as food preservatives. To our knowledge, this is the first report to confirm antimicrobial properties of essential oils derived from L. sibiricus root.
The next step in our study was to evaluate the anti-inflammatory activities of essential oils from L. sibiricus root on NHA cells. This effect has been proven by ELISA examination of LPS-induced production of pro- and anti-inflammatory cytokines such as TNF-[alpha], IL-6, IL-2, IL-4, IL-1[beta], and INF-[gamma] and treatment with essential oils. Additionally, the study examined the expression of genes such as TNF-[alpha], IL-6, IL-2, IL-4, IL-1[beta], and INF-[gamma] on mRNA level. As inflammation is an important aspect of many human diseases, its reduction could be of therapeutic interest. Glial cells such as astrocytes and microglia are important immune cells in the brain . The activation of microglia as resident microphages initiates the release of several potentially cytotoxic substances including reactive oxygen intermediates, nitric oxide, and proteases . These components are believed to contribute to the progressive damage observed in neurodegenerative diseases; hence, reducing their levels in activated microglia can ameliorate neurodegenerative disease symptoms [42, 43]. Many cytokines and inflammatory factors, neurotransmitters, and neuropeptides are known to affect IL-6 regulation in brain cells. In addition, IL-1[beta] and TNF-[alpha] have been observed to induce IL-6 in cultured cortical neurons and astrocytes, the latter involving the NF[kappa]B and the PKC pathway . LPS has commonly been used as an inflammogen for activating cells in several in vivo and in vitro cell models . While LPS normally induces IL-6 in both astrocytes and microglia, TNF-[alpha] only induces IL-6 in astrocytes; it does not do so in microglia . Species-specific effects may exist, since, in human cells in vitro, LPS affects microglia to a greater degree than astrocytes with regard to the production of TNF-[alpha], IL-1[beta], and IL-6; however, IL-1[beta] is a potent stimulator of IL-6 production in astrocytes . Therefore, the inhibition of normal human astrocyte activation and reduction of the release of pro- and anti-inflammatory mediators represent a promising strategy for the prevention of neurodegenerative diseases. Our findings are the first to report that NR and HR essential oils from L. sibiricus root suppressed TNF-[alpha], IL-6, IL-2, IL-4, and IL-1[beta] production induced by LPS stimulation in a dose-dependent manner. Furthermore, essential oil was found to suppress the expression of TNF-[alpha], IL-6, IFN-[gamma], and IL-1[beta] genes in LPS-activated NHA cells. We suspect that this is due to the major compounds identified in the essential oils, that is, sesquiterpenes such as (E)-[beta]-caryophyllene and germacrene D; however, the literature indicates that this may be due to the synergistic effect of the constituents in the oil. Similarly, Baylac and Racine note that sesquiterpenes such as [beta]-caryophyllene exhibited activity in the in vitro 5-lipoxygenase assay in the inflammation process .
Our findings detail the constituents of the essential oil of normal and hairy roots (NR and HR) of L. sibiricus and indicate their promising use as pharmaceuticals, with antimicrobial, antioxidant, anti-inflammatory, and antiproliferative activities. Our results clearly show that these essential oils are active against grade IV glioma cells, inhibit the growth of food pathogens, have moderate antioxidant activity in ABTS test, and suppress LPS-stimulated NHA activation by inhibiting pro- and anti-inflammatory mediators. The results presented here can be considered as the first information on biological properties of NR and HR essential oils of L. sibiricus roots. Therefore, this plant may be used to safely extend the shelf life of food and may be an interesting candidate in phytotherapy.
The authors declare that there is no conflict of interests regarding the publication of this paper.
This work was financially supported by Medical University of Lodz, Poland (503/3-012-01/503-31-001).
 M. J. Balunas and A. D. Kinghorn, "Drug discovery from medicinal plants," Life Sciences, vol. 78, no. 5, pp. 431-441, 2005.
 L. Tripathi and J. N. Tripathi, "Role of biotechnology in medicinal plants," Tropical Journal of Pharmaceutical Research, vol. 2, no. 2, pp. 243-253, 2003.
 J. S. Rad, S. M. H. Alfatemi, and M. S. Rad, "In vitro assessment of antibacterial activity of Salicornia herbacea L. seed extracts against multidrug resistant grampositive and gram-negative bacteria," International Journal of Biosciences (IJB), vol. 4, no. 6, pp. 217-222, 2014.
 J. Sharifi-Rad, S. M. Hoseini-Alfatemi, M. Sharifi-Rad, and W. N. Setzer, "Chemical composition, antifungal and antibacterial activities of essential Oil from lallemantia royleana (benth. in wall.) benth," Journal of Food Safety, vol. 35, no. 1, pp. 19-25, 2015.
 E. Skala, P. Sitarek, M. Rozalski et al., "Antioxidant and DNA repair stimulating effect of extracts from transformed and normal roots of rhaponticum carthamoides against induced oxidative stress and DNA damage in CHO cells," Oxidative Medicine and Cellular Longevity, vol. 2016, Article ID 5753139, 11 pages, 2016.
 E. Skala, P. Sitarek, M. Toma et al., "Inhibition of human glioma cell proliferation by altered Bax/Bcl-2-p53 expression and apoptosis induction by Rhaponticum carthamoides extracts from transformed and normal roots," Journal of Pharmacy and Pharmacology, vol. 68, no. 11, pp. 1454-1464, 2016.
 D. M. Maestri, V. Nepote, A. L. Lamarque, and J. A. Zygadlo, "Natural products as antioxidants," in Phytochemistry: Advances in Research, F. Imperato, Ed., pp. 105-135, Research Signopost, Kerala, India, 2006.
 S. M. Pourmortazavi and S. S. Hajimirsadeghi, "Supercritical fluid extraction in plant essential and volatile oil analysis," Journal of Chromatography A, vol. 1163, no. 1-2, pp. 2-24, 2007
 O. I. Aruoma, "Free radicals, oxidative stress, and antioxidants in human health and disease," Journal of the American Oil Chemists' Society, vol. 75, no. 2, pp. 199-212,1998.
 G. P. P. Kamatou and A. M. Viljoen, "A review of the application and pharmacological properties of a-bisabolol and [alpha]-bisabolol-rich oils," Journal of the American Oil Chemists' Society, vol. 87, no. 1, pp. 1-7, 2010.
 M. A. Islam, F. Ahmed, A. K. Das, and S. C. Bachar, "Analgesic and anti-inflammatory activity of Leonurus sibiricus," Fitoterapia, vol. 76, no. 3-4, pp. 359-362, 2005.
 F. Ahmed, M. A. Islam, and M. M. Rahman, "Antibacterial activity of Leonurus sibiricus aerial parts," Fitoterapia, vol. 77, no. 4, pp. 316-317, 2006.
 H.-Y. Shin, S.-H. Kim, S.-M. Kang et al., "Anti-inflammatory activity of Motherwort (Leonurus sibiricus L.)," Immunopharmacology and Immunotoxicology, vol. 31, no. 2, pp. 209-213, 2009.
 M.-J. Lee, H.-S. Lee, S.-D. Park, H.-I. Moon, and W.-H. Park, "Leonurus sibiricus herb extract suppresses oxidative stress and ameliorates hypercholesterolemia in C57BL/6 mice and TNF-[alpha] induced expression of adhesion molecules and lectin-like oxidized LDL receptor-1 in human umbilical vein endothelial cells," Bioscience, Biotechnology and Biochemistry, vol. 74, no. 2, pp. 279-284, 2010.
 P. Sitarek, E. Skala, M. Toma et al., "A preliminary study of apoptosis induction in glioma cells via alteration of the Bax/Bcl2-p53 axis by transformed and non-transformed root extracts of Leonurus sibiricus L.," Tumor Biology, vol. 37, no. 7, pp. 8753-8764, 2016.
 P. Sitarek, E. Skala, H. Wysokinska et al., "The effect of Leonurus sibiricus plant extracts on stimulating repair and protective activity against oxidative DNA damage in CHO cells and content of phenolic compounds," Oxidative Medicine and Cellular Longevity, vol. 2016, Article ID 5738193, 11 pages, 2016.
 E. Skala, R. Grabkowska, P. Sitarek, L. Kuzma, A. Blauz, and H. Wysokinska, "Rhaponticum carthamoides regeneration through direct and indirect organogenesis, molecular profiles and secondary metabolite production," Plant Cell, Tissue and Organ Culture, vol. 123, no. 1, pp. 83-98, 2015.
 J. Makowczynska, E. Sliwinska, D. Kalemba, E. Piatczak, and H. Wysokinska, "In vitro propagation, DNA content and essential oil composition of Teucrium scorodonia L. ssp. Scorodonia," Plant Cell, Tissue and Organ Culture, vol. 127, no. 1, pp. 1-13, 2016.
 R. P. Adams, Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy, Allured, Carol Stream, Ill, USA, 4th edition, 2007.
 D. Joulain and W. A. Konig, The Atlas of Spectral Data of Sesquiterpene Hydrocarbons, E.B.-Verlag, Hamburg, Germany, 1988.
 P. A. Wayne, Performance standards for antimicrobial susceptibility testing: Twenty Fifth International Supplement M100-S25, Clinical and Laboratory Standards Institute, 2015.
 M. S. Blois, "Antioxidant determinations by the use of a stable free radical," Nature, vol. 181, pp. 1199-1200, 1958.
 R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, "Antioxidant activity applying an improved ABTS radical cation decolorization assay," Free Radical Biology and Medicine, vol. 26, no. 9-10, pp. 1231-1237, 1999.
 M. G. Acimovic, S. I. Oljaca, V.V.Tesevic, M. M. Todosijevic, and J. N. Djisalov, "Evaluation of caraway essential oil from different production areas of Serbia," Horticultural Science, vol. 41, no. 3, pp. 122-130, 2014.
 K. Msaada, K. Hosni, M. B. Taarit, T. Chahed, M. E. Kchouk, and B. Marzouk, "Changes on essential oil composition of coriander (Coriandrum sativum L.) fruits during three stages of maturity," Food Chemistry, vol. 102, no. 4, pp. 1131-1134, 2007.
 A. G. Pirbalouti, B. Hamedi, P. F. Malek, E. Rahimi, and NR. Nasri, "Inhibitory activity of Iranian endemic medicinal plants against Vibrio parahaemolyticus and Vibrio harveyi," Journal of Medicinal Plants Research, vol. 5, no. 32, pp. 7049-7053, 2011.
 R. R. Pandey, R. C. Dubey, and S. Saini, "Phytochemical and antimicrobial studies on essential oils of some aromatic plants," African Journal of Biotechnology, vol. 9, no. 28, pp. 4364-4368, 2010.
 R. W. Bussmann and A. Glenn, "Medicinal plants used in Northern Peru for the treatment of bacterial and fungal infections and inflammation symptoms," Journal of Medicinal Plants Research, vol. 5, no. 8, pp. 1297-1304, 2011.
 J. S. Dambolena, M. P. Zunino, E. I. Lucini et al., "Essential oils of plants used in home medicine in north of Argentina," Journal of Essential Oil Research, vol. 21, no. 5, pp. 405-409, 2009.
 L. F. R. Almeida, M. E. A. Delachiave, and M. O. M. Marques, "Composiyao do oleo essencial de rubim (Leonurus sibiricus L. --Lamiaceae)," A Revista Brasileira de Plantas Medicinais, vol. 8, no. 1, pp. 35-38, 2005.
 S. Afoulous, H. Ferhout, E. G. Raoelison et al., "Chemical composition and anticancer, antiinflammatory, antioxidant and antimalarial activities of leaves essential oil of Cedrelopsis grevei," Food and Chemical Toxicology, vol. 56, pp. 352-362, 2013.
 M. Ennajar, J. Bouajila, A. Lebrihi et al., "Chemical composition and antimicrobial and antioxidant activities of essential oils and various extracts of Juniperus phoenicea L. (Cupressacees)," Journal of Food Science, vol. 74, no. 7, pp. M364-M371, 2009.
 M. Andrade, M. das Grayas Cardoso, J. de Andrade et al., "Chemical composition and antioxidant activity of essential oils from cinnamodendron dinisii schwacke and Siparuna guianensis aublet," Antioxidants, vol. 2, no. 4, pp. 384-397, 2013.
 J. Legault and A. Pichette, "Potentiating effect of [beta]-caryophyllene on anticancer activity of a-humulene, isocaryophyllene and paclitaxel," Journal of Pharmacy and Pharmacology, vol. 59, no. 12, pp. 1643-1647, 2007
 P. F. de Oliveira, J. M. Alves, J. L. Damasceno et al., "Cytotoxicity screening of essential oils in cancer cell lines," Brazilian Journal of Pharmacognosy, vol. 25, no. 2, pp. 183-188, 2015.
 L. Xiong, C. Peng, Q.-M. Zhou et al., "Chemical composition and antibacterial activity of essential oils from different parts of Leonurus japonicus houtt," Molecules, vol. 18, no. 1, pp. 963-973, 2013.
 F. Sharopov, M. Braun, I. Gulmurodov, D. Khalifaev, S. Isupov, and M. Wink, "Antimicrobial, antioxidant, and anti-inflammatory activities of essential oils of selected aromatic plants from tajikistan," Foods, vol. 4, no. 4, pp. 645-653, 2015.
 S. S. Dahham, Y. M. Tabana, M. A. Iqbal et al., "The anticancer, antioxidant and antimicrobial properties of the sesquiterpene [beta]-caryophyllene from the essential oil of Aquilaria crassna," Molecules, vol. 20, no. 7, pp. 11808-11829, 2015.
 I. Neto, J. Andrade, A. S. Fernandes et al., "Multicomponent petasis-borono mannich preparation of alkylaminophenols and antimicrobial activity studies," ChemMedChem, vol. 11, no. 18, pp. 2015-2023, 2016.
 T. Wyss-Coray and L. Mucke, "Inflammation in neurodegenerative disease--a double-edged sword," Neuron, vol. 35, no. 3, pp. 419-432, 2002.
 G. J. Harry and A. D. Kraft, "Neuroinflammation and microglia: considerations and approaches for neurotoxicity assessment," Expert Opinion on Drug Metabolism and Toxicology, vol. 4, no. 10, pp. 1265-1277, 2008.
 A. D. Kraft and G. Jean Harry, "Features of microglia and neuroinflammation relevant to environmental exposure and neurotoxicity," International Journal of Environmental Research and Public Health, vol. 8, no. 7, pp. 2980-3018, 2011.
 B. Liu and J.-S. Hong, "Role of microglia in inflammation-mediated neurodegenerative diseases: mechanisms and strategies for therapeutic intervention," Journal of Pharmacology and Experimental Therapeutics, vol. 304, no. 1, pp. 1-7, 2003.
 S. C. Lee, W. Liu, D. W. Dickson, C. F. Brosnan, and J. W. Berman, "Cytokine production by human fetal microglia and astrocytes: differential induction by lipopolysaccharide and IL-1[beta]," Journal of Immunology, vol. 150, no. 7, pp. 2659-2667, 1993.
 H.-M. Gao, J. Jiang, B. Wilson, W. Zhang, J.-S. Hong, and B. Liu, "Microglial activation-mediated delayed and progressive degeneration of rat nigral dopaminergic neurons: relevance to Parkinson's disease," Journal of Neurochemistry, vol. 81, no. 6, pp. 1285-1297, 2002.
 J. G. Norris, L.-P. Tang, S. M. Sparacio, and E. N. Benveniste, "Signal transduction pathways mediating astrocyte IL-6 induction by IL-1[beta] and tumor necrosis factor-[alpha]," Journal of Immunology, vol. 152, no. 2, pp. 841-850, 1994.
 M. Sawada, A. Suzumura, and T. Marunouchi, "TNF[alpha] induces IL-6 production by astrocytes but not by microglia," Brain Research, vol. 583, no. 1-2, pp. 296-299, 1992.
 S. Baylac and P. Racine, "Inhibition of 5-lipoxygenase by essential oils and other natural fragment extracts," International Journal of Aromatherapy, vol. 13, no. 2-3, pp. 138-142, 2003.
Przemyslaw Sitarek, (1) Patricia Rijo, (2,3) Catarina Garcia, (2) Ewa Skala, (1) Danuta Kalemba, (4) Adam J. Bialas,5 Janusz Szemraj, (6) Dariusz Pytel, (7) Monika Toma, (8) Halina Wysokinska, (1) and Tomasz Sliwinski (8)
(1) Department of Biology and Pharmaceutical Botany, Medical University of Lodz, Lodz, Poland
(2) Center for Research in Biosciences & Health Technologies (CBIOS), Universidade Lusofona de Humanidades e Tecnologias, 1749-024 Lisboa, Portugal
(3) Instituto de Investigacao do Medicamento (iMed.ULisboa), Faculdade de Farmacia, Universidade de Lisboa, 1649-003 Lisboa, Portugal
(4) Institute of General Food Chemistry, Lodz University of Technology, Lodz, Poland
(5) Department of Pneumology and Allergy, 1st Chair of Internal Medicine, Medical University of Lodz, Lodz, Poland
(6) Department of Medical Biochemistry, Medical University of Lodz, Lodz, Poland
(7) Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Hollings Cancer Center, HCC-709, 86 Jonathan Lucas Street, Charleston, SC 29425, USA
(8) Department of Molecular Genetics, University of Lodz, Lodz, Poland
Correspondence should be addressed to Przemyslaw Sitarek; email@example.com
Received 23 September 2016; Accepted 7 December 2016; Published 16 January 2017
Academic Editor: Chung-Yen Oliver Chen
Caption: Figure 1: Viability of NHA cells (a) and IV grade glioma cells (b) treated with NR or HR essential oil from L. sibiricus root for 24 h. The values are the mean [+ or -] SD for triplicate. *p < 0.05 versus control. *p < 0.05 NR versus HR.
Caption: Figure 2: Effect of NR and HR essential oil of L. sibiricus root on the production of cytokines IL-6, IL-1[beta], TNF-[alpha], IL-2, IL-4, and IFN-[gamma] by LPS induced in NHA cells in various concentrations (15-60 [micro]g/mL). The supernatants were collected and analysed by ELISA kits. The data are presented as mean [+ or -] SD of three independent experiments. < 0.05: treated group significantly different from LPS-treated group. *p < 0.05 NR versus HR.
Caption: Figure 3: Impact of NR and HR essential oil of L. sibiricus root on gene expression. NHA cells were treated with LPS and next NR and HR essential oil for 24 hours. (a) The bar graph gives the relative density. (b) Expression of mRNA levels of IL-1[beta], IL-2, IL-4, IL6, IFN-[gamma], and TNF-[alpha] genes. mRNA values are mean [+ or -] SD, < 0.05 compared to LPS group. Data were obtained as the mean of three replications.
Table 1: Constituents of essential oils from NR and HR Leonurus sibiricus root. Peak number Constituent [RI.sub.exp] (1) Hexanal 771 (2) Hexanol 841 (3) Heptanal 879 (4) Benzaldehyde 936 (5) [alpha]-Pinene 930 (6) Oct-1-en-3-one 952 (7) Oct-1-en-3-ol 961 (8) [beta]-Pinene 972 (9) Pentylfuran 975 (10) Octan-3-ol 983 (11) Myrcene 982 (12) Phenylacetaldehyde 1009 (13) p-Cymene 1011 (14) [beta]-Phellandrene 1019 (15) Limonene 1022 (16) (Z)-[beta]-Ocimene 1028 (17) (E)-Oct-2-enal 1032 (18) (E)-[beta]-Ocimene 1038 (19) p-Cymenene 1072 (20) Terpinolene 1078 (21) Nonanal 1072 (22) Linalool 1083 (23) Menthone 1132 (24) (E)-Non-2-enal 1135 (25) Nonanol 1147 (26) Borneol 1148 (27) p-Cymen-9-ol 1156 (28) Terpinen-4-ol 1157 (29) Methyl salicylate 1168 (30) [alpha]-Terpineol 1171 (31) Safranal 1176 (32) Decanal 1181 (33) [beta]-Cyclocitral 1192 (34) Trans-chrysanthenyl acetate 1213 (35) Thymol 1255 (36) Decanol 1260 (37) Bornylacetate 1266 (38) Undecanal 1285 (39) (2E,4E)-Deca-2,4-dienal 1286 (40) Bicycloelemene 1336 (41) [beta]-Damascenone 1361 (42) [alpha]-Ylangene 1373 (43) [alpha]-Copaene# 1376 (44) [beta]-Bourbonene 1383 (45) [beta]-Elemene 1389 (46) Myli-4(15)-ene# 1411 (47) (E)-[beta]-Caryophyllene# 1419 (48) [beta]-Duprezianene 1427 (49) [alpha]-Guaiene 1443 (50) Isogermacrene D 1447 (51) (E)-[beta]-Farnesene 1447 (52) [alpha]-Humulene 1452 (53) [beta]-Ionone 1465 (54) [gamma]-Gurjunene 1469 (55) Selina-4,7-diene# 1469 (56) Selina-4,11-diene# 1475 (57) Germacrene D# 1477 (58) cis-[beta]-Guaiene 1482 (59) [beta]-Selinene 1484 (60) Pentadec-1-ene 1488 (61) Bicyclogermacrene 1492 (62) [alpha]-Muurolene 1492 (63) (E,E)-[alpha]-Farnesene 1494 (64) [gamma]-Cadinene 1506 (65) Guaia-1(10),11-diene# 1515 (66) [delta]-Cadinene 1519 (67) trans-Calamenene 1517 (68) [alpha]-Calacorene 1527 (69) (E)-[alpha]-Bisabolene 1531 (70) Nerolidol 1546 (71) Guaia-6,9-dien-4-[beta]-ol 1564 (72) Spathulenol 1567 (73) Caryophyllene epoxide# 1570 (74) Humulene epoxide II 1598 (75) [alpha]-Corocalene 1600 (76) Caryophylla-3(15),7(14)-dien-6-ol 1620 (77) T-Muurolol 1625 (78) [beta]-Eudesmol 1635 (79) [alpha]-Cadinol 1637 (80) Cadalene 1646 (81) Myristic acid 1742 (82) Farnesylacetone 1888 (83) Methyl palmitate 1902 (84) Palmitic acid 1945 (85) Phytol 2095 Total identified Monoterpene hydrocarbons MH Oxygenated monoterpenes MO Sesquiterpene hydrocarbons SH Oxygenated sesquiterpenes SO Other O Peak number [RI.sub.lit] NR HR Class of compounds (1) 770 0.4 0.3 O (2) 837 t 0.1 O (3) 879 0.2 0.1 O (4) 941 t t O (5) 936 3.2 1.0 MH (6) 956 0.3 0.3 O (7) 962 3.5 3.6 O (8) 978 0.5 0.1 MH (9) 977 0.9 0.8 O (10) 981 t 0.2 O (11) 987 0.4 0.1 MH (12) 1012 t t O (13) 1015 0.1 t MH (14) 1023 0.1 t MH (15) 1025 0.9 0.3 MH (16) 1029 0.6 t MH (17) 1034 -- 0.1 O (18) 1141 0.2 0.1 O (19) 1075 0.1 t MH (20) 1082 0.1 0.0 MH (21) 1176 0.3 0.5 O (22) 1186 0.5 0.4 MO (23) 1136 0.1 0.1 MO (24) 1139 0.3 0.4 O (25) 1149 t t MO (26) 1150 t 0.1 O (27) 1157 0.1 0.1 MO (28) 1158 0.5 0.1 MO (29) 1171 0.1 0.1 O (30) 1176 0.3 0.2 MO (31) 1182 0.0 0.1 MO (32) 1180 0.4 0.7 O (33) 1195 0.1 0.1 MO (34) 1217 0.1 0.1 MO (35) 1267 0.3 0.6 MO (36) 1264 -- 0.1 O (37) 1270 0.1 0.1 MO (38) 1290 0.1 0.2 O (39) 1291 0.1 0.1 O (40) 1338 1.7 3.0 SH (41) 1363 0.5 0.9 O (42) 1376 0.3 t SH (43) 1379 0.4 5.0# SH (44) 1386 0.2 3.0 SH (45) 1389 1.7 0.5 SH (46) 1418 6.4# 0.5 SH (47) 1421 7.3 22.6# SH (48) 1423 0.7 1.1 SH (49) 1440 1.6 -- SH (50) 1445 -- 0.2 SH (51) 1448 -- 0.1 SH (52) 1455 2.2 3.3 SH (53) 1468 0.2 0.5 O (54) 1472 0.5 -- SH (55) 1470 9.7# -- SH (56) 1475 4.4# -- SH (57) 1479 1.2# 19.8# SH (58) 1488 1.3 -- SH (59) 1486 9.9# 0.9 SH (60) 1486 -- 0.7 O (61) 1494 -- 2.1 SH (62) 1496 2.1 -- SH (63) 1498 -- 0.6 SH (64) 1507 0.2 0.2 SH (65) 1517 5.9# -- SH (66) 1520 -- 1.1 SH (67) 1517 0.3 0.1 SH (68) 1528 0.3 0.1 SH (69) 1530 1.2 1.1 SH (70) 0.3 0.5 SO (71) 1465 0.5 -- SO (72) 1572 -- 1.6 SO (73) 1578 4.5# 8.0# SO (74) 1602 0.6 1.2 SO (75) 1.2 -- SH (76) 1636 0.4 0.4 SO (77) 1633 0.2 0.3 SO (78) 1641 0.9 0.9 SO (79) 1643 0.4 0.5 SO (80) 1659 t -- SH (81) 1748 0.3 0.2 O (82) 1890 0.1 0.2 O (83) 1901 0.1 0.2 O (84) 1942 2.4 2.2 O (85) 2114 0.4 1.3 O 86.5 96.1 6.0 1.5 2.1 1.9 60.2 64.8 6.8 13.4 10.8 14.0 The concentrations of the main compounds were signified in bold. RI: relative retention index on Rtx-1 ms column, [RI.sub.lit]: relative retention index of literature, NR: essential oil of normal roots of L. sibiricus, and HR: essential oils of hairy roots of L. sibiricus. Note: The concentrations of the main compounds were signified in bold indicated with #. Table 2: Antimicrobial activity of the essential oils obtained from NR and HR roots of L. sibiricus. Staphylococcus aureus Pseudomonas aeruginosa Plant material MIC MBC MIC MBC ([micro]g/mL) ([micro]g/mL) HR essential oil 250 >500 125 >500 NR essential oil 250 >500 125 >500 Positive control 7.82 >500 <0.48 >500 VAN NOR Escherichia coli Enterococcus faecalis Plant material MIC MBC MIC MBC ([micro]g/mL) ([micro]g/mL) HR essential oil 250 >500 250 >500 NR essential oil 250 >500 250 >500 Positive control 0.98 >500 1.95 >500 NOR VAN Saccharomyces cerevisiae Candida albicans Plant material MIC MBC/MFC MIC MBC/MFC ([micro]g/mL) ([micro]g/mL) HR essential oil 1250 2500 1250 >5000 NR essential oil 2500 >5000 1250 1250 Positive control <0.48 >5000 <0.48 >5000 ANF ANF VAN: Vancomycin, NOR: Norfloxacin, ANF: Amphotericin B. Data represent the median values in triplicate. Table 3: Antioxidant activity of the essential oils obtained from NR and HR roots of L. sibiricus. Antioxidant activity Oil plant material ABTS ([EC.sub.50], [micro]g/mL) HR essential oil 88.22 [+ or -] 1.35 NR essential oil 92.40 [+ or -] 1.99 Positive control/BHA 6.25 [+ or -] 0.09 Antioxidant activity Oil plant material DPPH ([EC.sub.50], [micro]g/mL) HR essential oil 489.20 [+ or -] 1.45 NR essential oil 500.50 [+ or -] 1.37 Positive control/BHA 4.25 [+ or -] 0.10 BHA: butylated hydroxyl anisole, DPPH (2,2-diphenyl-l-picrylhydrazyl), and ABTS (2,20-azinobis-3-ethylbenzothiazoline-6-sulphonate). Data represent the mean values [+ or -] SD in triplicate.
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|Title Annotation:||Research Article|
|Author:||Sitarek, Przemyslaw; Rijo, Patricia; Garcia, Catarina; Skala, Ewa; Kalemba, Danuta; Bialas, Adam J.;|
|Publication:||Oxidative Medicine and Cellular Longevity|
|Date:||Jan 1, 2017|
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