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Antennal Sensilla in the Small Banana Weevil Polytus mellerborgi Boheman (Coleoptera: Curculionidae).

Byline: Jiong Yin, Yujie Wang, Fuping Lu, Jinglin Gao and Dongxiang Zhao

ABSTRACT

The antennal morphology and sensilla of small banana weevil, Polytus mellerborgi Boheman (Coleoptera: Curculionidae), were examined. The antennae of P. mellerborgi were composed of eight antennomeres, a scape, pedicel and flagellum composed of six flagellomeres. The use of scanning electron microscopy facilitated the recognition of five different sensilla types on the antennae of P. mellerborgi, including sensilla trichodea, sensilla chaetica (with two subtypes: Sch1 and Sch2), sensilla basiconica and sensilla gemmiformia. These results provided necessary background information for our ongoing study on electrophysiology and chemical ecology of P. mellerborgi.

Key words

Antennal club, small banana weevil.

INTRODUCTION

The banana weevils are recognized globally as the major pest of banana and plantain (Tinzaara et al., 2011), and Polytus mellerborgi Boheman has become widely spread (Zimmerman, 1968), which looks like a miniature Cosmopolites sordidus Germar (Coleoptera: Curculionidae) and therefore commonly known as the small banana weevil (Okolle et al., 2009). P. mellerborgi is often found in numbers in decaying trunks of bananas and bores in the decaying corms and stalks of banana (Zimmerman, 1968; Yin et al., 2015). Although it is more in the field followed by C. sordidus, damage caused by P. mellerborgi is yet to be assessed (Okolle et al., 2009).

Many studies on other banana weevils show that traps baited with plant volatiles and/or aggregation pheromones may have great potential as an efficient monitoring and control tool against these pests (Jayaraman et al., 1997). So far, we haven't been sure if the small banana weevils are attracted to plant volatiles emanating from crop residues as the same as C. sordidus (Budenberg et al., 1993), although P. mellerborgi may be trapped overnight by placing freshly cut pseudostem on the ground, with the cut surface in contact with the soil (Nelson et al., 2006). Apparently, the olfactory and taste sensilla located on the insect antennae are involved in the process of damaging host plants. However, there has been no published work on the antennal structure of P. mellerborgi. Therefore, the aim of this study is to identify the different sensilla types of P. mellerborgi using scanning electron microscopy (SEM).

It could provide basic information for developing methods of pest control in terms of olfactory and contact chemosensory systems.

MATERIALS AND METHODS

P. mellerborgi adults were obtained in Changjiang (N1932', E10895'), Hainan Province, P. R. China. For SEM observation, 10 antennae of weevils were cut randomly regardless of their sexes. After that, the antennae were rinsed in 70 % ethanol in ultrasonic cleaning instrument (JL-, Jieli Co. Ltd., Shanghai City, P. R. China) for 1 min and then dehydrated in a graded ethanol series (80, 90, 95, 100%). After air drying, the specimens were mounted on a holder using electric adhesive tape and then coated with gold and observed using HITACHI S-3000N SEM (Hitachi Corp., Tokyo, Japan) in Hainan University (Haikou City, Hainan Province, P. R. China). Five antennae on the ventral side and five on the dorsal side were studied under SEM. Thirty images of the sensilla on the surfaces of the antennae were taken at magnification of 180x to 9000x, EB acceleration voltage at 10.0 kV and image size ranging from 9.7 to 18.5 mm, then classified and measured.

In order to calculate the mean length of sensilla types, at least 10 sensilla of the same type from different individuals were measured.

The terminology and classification of sensilla used here followed those of Schneider (1964) and Keil (1997). The length and width of different types of sensilla were measured using Nano Measurer 1.2 software (Department of Chemistry, Fudan University, P. R. China). The data were analyzed using SPSS19.0 for Windows (SPSS Inc., Chicago, USA). Statistical results were expressed as mean standard deviation.

RESULTS

The antennae of P. mellerborgi were composed of eight antennomeres, a scape, pedicel and flagellum composed of six flagellomeres. The last flagellomere was called as antennal club. The flagellum was the most prominent part on the antenna, on which most sensilla were located, especially on the antennal club. Five types of sensilla and cuticular grooves were found on P. mellerborgi antennae according to SEM, including sensilla trichodea, sensilla chaetica type 1 and 2, sensilla basiconica, and sensilla gemmiformia. These cuticular grooves occurred widely on the antennae of adult weevils (Fig.1D and 1E).

Sensilla trichodea (St.)

St were on the antennal club and towards the club apex, and identified based on their length and shape with a conical base and slightly curved tip (Fig.1B and 1C). They were distributed widely over the entire antennal club and the most extensively distributed type of sensilla on the antennae of P. mellerborgi in various lengths. These sensilla varied from 25.70 to 36.76 um in length, and from 1.67 to 2.77 um in width (Table I).

Table I.- Sizes of antennal sensilla of P. mellerborgi.

Sensilla type###Length (um)###Width (um)

sensilla trichodea###31.235.53###2.220.55

sensilla chaetica 1###48.754.89###2.070.38

sensilla chaetica 2###26.5519.59###1.650.92

sensilla basiconica###16.310.03###1.120.02

sensilla gemmiformia###0.440.10###0.450.09

Sensilla chaetica (Sch.)

The sensilla chaetica, the longest hairs on the antennae, were divided into two subtypes. Sch1, long, robust, bristle-like sensilla with a sharp tip, sat on the antennal club (Fig.1B). Sch2, long, robust, bristle-like sensilla, extended from the antennae at roughly a 20 angle and had shallow longitudinal veins and irregular tips (Fig.1B, 1D and 1E). Sch2 were distributed regularly along the lateral side of flagellomeres.

Sensilla basiconica (Sb)

Sensilla basiconica (Fig.1C), common and distributed randomly on antennal club, had fewer numbers with smooth wall. Sb were blunt-tipped, shorter than St, mean length was 16.310.03 um and mean width was 1.120.02 um (Table I).

Sensilla gemmiformia (Sg)

The sensilla gemmiformia (Fig. 1F) were only found in the joint between each two neighboring flagellomeres. Their appearance resembled a sawtooth. Sg were very small with a sharp tip and thick base.

DISCUSSION

The study demonstrated that P. mellerborgi contained five different types of sensilla on the antennae. Compared with other Curculionidae, P. mellerborgi antennae are similar to those of Odoiporus longicollis (Olivier) (Coleoptera: Curculionidae) (Gao et al., 2011), on both of which sensilla trichodea, sensilla chaetica, sensilla basiconica and sensilla gemmiformia are all found. The antennal club of P. mellerborgi, only one flagellomere, was absolutely different from that of Hypera meles (F.) (Coleoptera: Curculionidae) which is formed by four flagellomeres (Smith et al., 1976), Eucryptorrhynchus chinensis (Olivier) and E. brandti (Coleoptera: Curculionidae) both of which are composed of three flagellomeres (Yu et al., 2013). St of P. mellerborgi widely distributed, which were more densely concentrated on the distal region of the antennal club.

In general, St are usually the most common and numerous structures on insect antennae and presumed to function as olfactory receptors in many insects (Bleeker et al., 2004; Mustaparta, 1975), for example, Agriotes obscurus L. (Coleoptera: Elateridae) (Merivee et al., 1997) and Psacothea hilaris (Pascoe) (Coleoptera: Cerambycidae) (Castrejon-Gomez et al., 1999).

External shape, large measurements, specific location at the antennal apex, small number and perpendicular position on the antennal surface indicate that Sch1 in P. mellerborgi are most probably taste sensilla which are crucial in final host plant detection and selection. Presumably the Sch1 are the first sensilla to contact with the substrate because they are the longest (protrude over all other sensilla) and project at right angles from the antennal surface. They also occur on the antennae of all beetles studied, for example, Carabidae (Merivee et al., 2000, 2001, 2002), Elateridae (Merivee et al., 1997, 1998, 1999) and many other beetles. By electrophysiological recordings these sensilla are innervated by five neurons: four chemoreceptor neurons and one mechanoreceptor neuron. Similar set of neurons is typical for insect taste sensilla.

In Carabidae, one of the neurons is sensitive to water soluble salts (Merivee et al., 2004), the second neuron responds to pH (Merivee et al., 2005; Milius et al., 2006), the third neuron responds to plant sugars and amino acids (Merivee et al., 2007, 2008, 2012), the fourth neuron is sensitive to plant secondary compounds like alkaloids and glucosides (Milius et al., 2011). The fifth, mechanoreceptor neuron responds to touch (Merivee et al., 2008). In Elateridae, three neurons have been electrophysiologically identified in these taste sensilla: the salt, the sugar and the touch sensitive neuron (Tooming et al., 2012). Sch2 of P. mellerborgi are probably mechanoreceptor located in a wide articular socket and responding to touch.

Sb have been reported on the antennae of several coleopteran species (Dyer and Seabrook, 1975; Alm and Hall, 1986; Dai and Honda, 1990). And they have been shown to function as olfactory chemoreceptors in several species of Coleoptera, such as Hylobius abietis L. (Coleoptera: Curculionidae) (Mustaparta, 1975). Lopes et al. (2002) reported that Sb in Phoracantha semipunctata Fabricius (Coleoptera: Cerambycidae) are structurally adapted to play a functional role in olfactory recognition of plant odours. Although Sg are not common in Coleoptera, which have been not found in Carabidae (Merivee et al., 2000, 2001, 2002) and Elateridae (Merivee et al., 1997, 1998, 1999), it means possible that Sg are candidate mechanoreceptors. And Sg reported in here were totally different from Anoplistes halodendri halodendri and A. halodendri ephippium (Coleoptera: Cerambycidae) (Liu et al., 2012).

In addition, sensilla coeloconic and sensilla styloconicum on most Coleoptera insects (Okada et al., 1992; Zacharuk, 1980) were not found on the antennae of P. mellerborgi. And sensilla placodea are usually distributed on the antennae of Curculionidae (Yan et al., 2011), which have not been discovered on the antennae of small banana weevils.

In conclusion, the presence of these sensilla types on the antennae probably suggests the importance of chemoreception in the behavioral ecology of P. mellerborgi. These results provide necessary background information on chemical ecology of P. mellerborgi although sensilla functions will require further study by electrophysiological and behavioral investigations.

ACKNOWLEDGMENTS

This study was financially supported by National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2007BAD48B05-01) and National Nonprofit Institute Research Grant of CATAS-EPPRI (2008hzs1J008).

Conflict of interest declaration

The authors have declared that no conflicts of interest exist.

REFERENCES

Alm, S. R. and Hall, F. R., 1986. Antennal sensory structures of Conotrachelus nenuphar (Coleoptera: Curculionidae). Annls. entomol. Soc. Am., 79: 324-333.

Bleeker, M. A. K., Smid, H. M., Van Aelst, A. C., Van Loon, J. J. A. and Vet, L. E. M., 2004. Antennal sensilla of two parasitoid wasps: A comparative scanning electron microscopy study. Microsc. Res. Tech., 63: 266-273.

Budenberg, W. J., Ndiege, I. O., Karago, F. W. and Hansson, B. S., 1993. Behavioural and electrophysiological responses of the banana weevil, Cosmopolites sordidus to host plant volatiles. J. chem. Ecol., 19: 267-272.

Castrejon-Gomez, V. R., Valdez-Carrasco, J., Cibrian-Tovar, J., Camino-Lavin, M. and Osorio, R., 1999. Morphology and distribution of the sense organs on the antennae of Copitarsia consueta (Lepidoptera: Noctuidae). Fla. Entomol., 82: 546-555.

Dai, H. G. and Honda, H., 1990. Sensilla on the antennal flagellum of the yellow spotted longicorn beetle, Psacothea hilaris (Pascoe) (Coleoptera: Cerambycidae). Appl. Ent. Zool., 25: 273-282.

Dyer, L. J. and Seabrook, W. D., 1975. Sensilla on the antennal flagellum of the sawyer beetles Monochamus notatus (Drury) and Monochamus scutellatus (Say) (Coleoptera: Cerambycidae). J. Morphol., 146: 513-531.

Gao, J-L., Yin, J., Zhao, D-X., Wang, Y-J. and Jiang, J., 2011. Scanning electron microscopic observations of banana pseudostem weevil, Odoiporus longicollis Olivier (Coleoptera: Curculionidae) antennal sensilla. Chin. J. Trop. Crops, 32: 471-474 (in Chinese).

Jayaraman, S., Ndiege, I. O., Oehlschlager, A. C., Gonzalez, L. M., Alpizar, D., Falles, M., Budenberg, W. J. and Ahuya, P., 1997. Synthesis, analysis and field activity of sordidin-A male-produced aggregation pheromone of the banana weevil, Cosmopolites sordidus (Germar), (Coleoptera: Curculionidae). J. chem. Ecol., 23: 1145-1161.

Keil, T. A., 1997. Comparative morphogenesis of sensilla: A review. Int. J. Insect Morphol. Embryol., 26: 151-160.

Liu, X. H., Luo, Y. Q., Cao, C. J. and Zong, S. X., 2012. Scanning electron microscopy of antennal sensible of Anoplistes halodendri halodendri and Anoplistes halodendri ephippium (Coleoptera: Cerambycidae). Microsc. Res. Tech., 75: 367-373.

Lopes, O., Barata, E. N., Mustaparta, H. and Araujo, J., 2002. Fine structure of antennal sensilla basiconica and their detection of plant volatiles in the eucalyptus woodborer, Phoracantha semipunctata Fabricius (Coleoptera: Cerambycidae). Arthrop. Struct. Dev., 31: 1-13.

Merivee, E., Rahi, M. and Luik, A., 1997. Distribution of olfactory and some other antennal sensilla in the male click beetle Agriotes obscurus L. (Coleoptera: Elateridae). Int. J. Insect Morphol. Embryol., 26: 75-83.

Merivee, E., Rahi, M., Bresciani, J., Ravn, H. P. and Luik, A., 1998. Antennal sensilla of the click beetle, Limonius aeruginosus (Olivier) (Coleoptera: Elateridae). Int. J. Insect Morphol. Embryol., 27: 311-318.

Merivee, E., Rahi, M. and Luik, A., 1999. Antennal sensilla of the click beetle, Melanotus villosus (Geoffroy) (Coleoptera: Elateridae). Int. J. Insect Morphol. Embryol., 28: 41-51.

Merivee, E., Ploomi, A., Rahi, M., Luik, A. and Sammelselg, V., 2000. Antennal sensilla of the ground beetle Bembidion lampros Hbst (Coleoptera, Carabidae). Acta Zool., 81: 339-350.

Merivee, E., Ploomi, A., Luik, A., Rahi, M. and Sammelselg, V., 2001. Antennal sensilla of the ground beetle Platynus dorsalis (Pontoppidan, 1763) (Coleoptera, Carabidae). Microsc. Res. Tech., 55: 339-349.

Merivee, E., Ploomi, A., Rahi, M., Bresciani, J., Ravn, H. P., Luik, A. and Sammelselg, V., 2002. Antennal sensilla of the ground beetle Bembidion properans Steph. (Coleoptera, Carabidae). Micron, 33: 429-440.

Merivee, E., Renou, M., Mand, M., Luik, A., Heidemaa, M. and Ploomi, A., 2004. Electrophysiological responses to salts from antennal chaetoid taste sensilla of the ground beetle Pterostichus aethiops. J. Insect Physiol., 50: 1001-1013.

Merivee, E., Ploomi, A., Milius, M., Luik, A. and Heidemaa, M., 2005. Electrophysiological identification of antennal pH receptors in the ground beetle Pterostichus oblongopunctatus. Physiol. Ent., 30: 122-133.

Merivee, E., Must, A., Milius, M. and Luik, A., 2007. Electrophysiological identification of the sugar cell in antennal taste sensilla of the predatory ground beetle Pterostichus aethiops. J. Insect Physiol., 53: 377-384.

Merivee, E., Martmann, H., Must, A., Milius, M., Williams, I. and Mand, M., 2008. Electrophysiological responses from neurons of antennal taste sensilla in the polyphagous predatory ground beetle Pterostichus oblongopunctatus (Fabricius 1787) to plant sugars and amino acids. J. Insect Physiol., 54: 1213-1219.

Merivee, E., Must, A., Tooming, E., Williams, I. and Sibul, I., 2012. Sensitivity of antennal gustatory receptor neurones to aphid honeydew sugars in the carabid Anchomenus dorsalis. Physiol. Ent., 37: 369-378.

Milius, M., Merivee, E., Williams, I., Luik, A., Mand, M. and Must, A., 2006. A new method for electrophysiological identification of antennal pH receptor cells in ground beetles: the example of Pterostichus aethiops (Panzer, 1796) (Coleoptera, Carabidae). J. Insect Physiol., 52: 960-967.

Milius, M., Merivee, E., Must, A., Tooming, E., Williams, I. and Luik, A., 2011. Electrophysiological responses of the chemoreceptor neurones in the antennal taste sensilla to plant alkaloids and glucosides in a granivorous ground beetle. Physiol. Ent., 36: 368-378.

Mustaparta, H., 1975. Responses of single olfactory cells in the pine weevil Hylobius abietis L. (Col.: Curculionidae). J. Comp. Physiol., 97: 271-290.

Nelson, S. C., Ploetz, R. C. and Kepler, A. K., 2006. Musa species (banana and plantain). Species Profiles for Pacific Island Agroforestry, 9-22.

Okada, K., Mori, M., Shimazaki, K. and Chuman, T., 1992. Morphological studies on the antennal sensilla of the cigarette beetle, Lasioderma serricorne (F) (Coleoptera: Anobiidae). Appl. Ent. Zool., 27: 269-276.

Okolle, J. N., Fansi, G. H., Lombi, F. M., Sama Lang, P. and Loubana, P. M., 2009. Banana entomological research in Cameroon: how far and what next. Afr. J. Pl. Sci. Biotechnol., 3: 1-19.

Schneider, D., 1964. Insect antennae. Annu. Rev. Ent., 9: 103-122.

Smith, C. M., Frazier, J. L., Coons, L. B. and Knight, W. E., 1976. Antennal sensilla of the clover head weevil Hypera meles (F.) (Coleoptera: Curculionidae). Int. J. Insect Morphol. Embryol., 5: 349-355.

Tinzaara, W., Gold, C. S., Dicke, M., Van Huis, A. and Ragama, P. E., 2011. Effect of age, female mating status and density on the banana weevil response to aggregation pheromone. Afr. Crop Sci. J., 19: 105-116.

Tooming, E., Merivee, E., Must, A., Luik, A. and Williams, I. H., 2012. Antennal sugar sensitivity in the click beetle Agriotes obscurus. Physiol. Ent., 37: 345-353.

Yan, S. C., Meng, Z. J., Peng, L. and Liu, D., 2011. Antennal sensilla of the pine weevil Pissodes nitidus Roel. (Coleoptera: Curculionidae). Microsc. Res. Tech., 74: 389-396.

Yin, J., Wang, Y. J., Gao, J. L. and Zhao, D. X., 2015. Survey on occurrence and damage of banana weevils in Hainan province, China. Biolife, 3: 662-664.

Yu, Q. Q., Liu, Z. K., Chen, C. and Wen, J., 2013. Antennal sensilla of Eucryptorrhynchus chinensis (Olivier) and Eucryptorrhynchus brandti (Harold) (Coleoptera: Curculionidae). Microsc. Res. Tech., 76: 968-978.

Zacharuk, R. Y., 1980. Ultrastructure and function of insect chemosensilla. Annu. Rev. Ent., 25: 27-47.

Zimmerman, E. C., 1968. Rhynchophorinae of southeastern Polynesia (Coleoptera: Curculionidae). Pac. Insects, 10: 47-77.
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Author:Dongxiang Zhao; Jiong Yin; Yujie Wang; Fuping Lu; Jinglin Gao
Publication:Pakistan Journal of Zoology
Article Type:Report
Geographic Code:9PAKI
Date:Apr 30, 2016
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