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Ann Allergy Asthma Immunol. 2014: berberine and limonin suppress IgE production by human b cells and peripheral blood mononuclear cells from food-allergic patients.

Background

Currently, there is no satisfactory treatment for IgE-mediated food allergy. Food Allergy Herbal Formula 2 (FAHF-2) and butanol-purified FAHF-2 (B-FAHF-2) have been shown to protect against peanut-induced

anaphylaxis and inhibit IgE synthesis in a murine model. Objective: To determine which herbs and compounds in FAHF-2 and B-FAHF-2 suppress IgE production.

Methods

The effect of FAHF-2 and B-FAHF-2 on IgE production was determined using a human B-cell line (U266). Individual compounds were isolated and identified using column chromatography, liquid chromatographic mass spectrometry, and nuclear magnetic resonance techniques. The potency of compounds on IgE suppression were investigated using U266 cells and verified using human peripheral blood mononuclear cells (n = 25) from peanut-allergic patients. Epsilon germline transcript expression was determined. Phosphorylated IkBa level was analyzed using the In-Cell Western assay. The mRNA expression of signal transducer and activator of transcription-3, T-box transcription factor TBX21, interferon-y, forkhead box P3, GATA-binding protein 3, interleukin-10, and interleukin-5 also were analyzed using real-time polymerase chain reaction. Results FAHF-2 and B-FAHF-2 inhibited IgE production by U266 cells. B-FAHF-2 was 9 times more effective than FAHF-2. Two compounds that inhibited IgE production were isolated from Philodendron chinensis and identified as berberine and limonin. Berberine was more potent and inhibited IgE production by peripheral blood mononuclear cells by 80% at 0.62 pg/mL. Berberine significantly inhibited -germline transcript expression by peripheral blood mononuclear cells. Phosphorylated IkBa level was significantly suppressed and mRNA expressions of T-box transcription factor TBX21 and signal transducer and activator of transcription-3 were significantly increased by berberine.

Conclusion

Berberine and limonin mediated IgE suppression. The mechanism by which berberine modulates -germline transcript expression might be through regulating the phosphorylated IkBa level and the expressions of signal transducer and activator of transcription-3 and T-box transcription factor TBX21.

S1081-1206(14)00509-2. doi: 10.1016/j.anal.2014.07.021.

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Article Details
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Title Annotation:Herbal Medicine
Author:Yang, N.; Wang, J.; Liu, C.; Song, Y.; Zhang, S.; Zi, J.; Zhan, J.; Masilamani, M.; Cox, A.; Nowak-W
Publication:Journal of the Australian Traditional-Medicine Society
Date:Dec 1, 2014
Words:302
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