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Analysis of the MHC class II I-Ag7 binding peptides in NOD and NOD.E[A.sup.D] mice. (Medical Science and Health Poster Session 09:00 AM-10:00 AM).

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Insulin Dependent Diabetes Mellitus (IDDM) is an autoimmune disorder that destroys the body's ability to produce insulin, a molecule that regulates blood glucose levels. Destruction occurs when the T cell receptor and co-receptor molecules interact with an autoangtigen present in the binding cleft of an MHC class II molecule on the surface of host cell tissue. The T cell erroneously marks the autoantigen as being pathogenic, initiating a cascade that induces destruction of the non-regenerative insulin-producing beta cells of the pancreas. In non-obese diabetic (NOD) mice, the expression of only I-Ag7 MHC class II molecules is attributed to the spontaneous development of IDDM. The NOD.Ead mouse is genetically identical to the NOD mouse, except for the expression of I-E, an additional type of MHC class II molecule. This mouse strain is diabetes resistant. We hypothesize that the peptides presented by the I-Ag7 molecule differ when the I-E molecule is also present. To assess the I-E protective mechanism, peptide-bound MHC class II molecules were purified from a minimum of 35 animals from both the NOD and NOD.Ead mouse strains using affinity column chromatography. The eluent was treated with trifluoroacetic acid to release the peptides from the binding cleft of the MHC class II I-Ag7 molecules. The peptides were isolated using Amicon Centriplus[R] YM-10 centrifugation, devices. The presence of peptides has been confirmed via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS). Analysis of the MHC class II I-Ag7 bound peptides from the two mouse strains will reveal if the expression of I-E quantitatively or qualitatively affects antigen presentation.

MARISSA R. MATARRESE s03.MMATARRESE@WITTENBERG.EDU, (MATTHEW S. HANSON MHANSON@WITTENBERG.EDU), WITTENBERG UNIVERSITY, 508 1/2 N. WITTENBERG AV, SPRINGFIELD OH 45501
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Author:Matarrese, Marissa R.; Hanson, Matthew S.
Publication:The Ohio Journal of Science
Article Type:Brief Article
Geographic Code:1USA
Date:Mar 1, 2003
Words:285
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