Anaemia & expression levels of CD35, CD55 & CD59 on red blood cells in Plasmodium falciparum malaria patients from India.
In an effort to understand the pathogenesis of anaemia in Pf infection we studied the relationship between expression level of CD35, CD55 and CD59 with haemoglobin status in a group of malaria cases from three regions of India, namely Assam, Goa and Chennai.
Material & Methods
Blood samples were collected from 50 consecutive P. falciparum malaria cases attending malaria clinics [Regional Medical Research Centre, Dibrugarh, Assam (33 cases); Goa and Chennai field units of National Institute of Malaria Research, New Delhi (14 and 3 cases respectively)] in three regions of India viz, Assam (East), Goa (West) and Chennai (South) during 2007-2008. This study was approved by institutional ethics committee of Postgraduate Institute of Medical Education & Research, Chandigarh, and written informed consent was obtained from all the study subjects prior to collection of blood samples. Subjects were excluded from participation if there was evidence of other concomitant infections like TB, typhoid, history of haemolytic disorders, etc. or had a history of blood transfusion or antimalarial treatment 3 months before enrolment. To compare the results with normal population, 30 apparently healthy age matched individuals were included as controls from Assam. Giemsa stained thick and thin blood films were used for microscopic detection and identification of malarial parasites. Parasites were counted against 200 WBCs and the value converted to parasites per [micro]l of peripheral blood. Approximately 5 ml of venous blood was also collected in EDTA vials and processed for flowcytometric study following the method of Waitumbi et al (3) with slight modifications. In brief, fluorescent staining was performed using monoclonal antibodies (Becton Dickinson, Biosciences, USA) against cell surface receptors [anti-human CR1 (clone E11; WS No.: III 204), CD55 (clone IA10; WS No.: V BP352, S031) and CD59 (clone p282 (H19); WS No.: V S006)]. For each sample 1 [micro]l (each) of whole blood was put into 5 sample tubes containing 100 [micro]l of staining buffer (PBS with 2% BSA); 20 [micro]l of anti-human FITC conjugate of CR1 or CD55 or CD59 or unstained control were put separately in the sample tubes and incubated at room temperature in dark for 20-30 min. After incubation, RBCs were washed in 2 ml of staining buffer and re-suspended in 500 [micro]l of staining buffer and analyzed in flowcytometer. The FACScan flowcytometer (Becton Dickinson, USA) which was used for the measurement of expression studies was optimized using standard fluorescent beads. For acquisition and analysis RBCs were gated using logarithmic amplification of their forward and side scatter characteristics. FITC florescence was measured by FL1 detector using logarithm amplification.
Statistical analysis: Statistical analysis was carried out using SPSS v11.0 (Spss Inc., Chicago, IL, USA). Correlation between different variables like expression levels of RBC surface receptors, haemoglobin levels, age of patients, level of parasitemia, etc. were studied using Spearman's correlation coefficient. Analysis of Covariance (ANCOVA) was used to compare mean expression of CD35, CD55 and CD59 between malaria cases or controls and between malaria cases with haemoglobin less than 7 g/dl versus malaria cases with haemoglobin more than 7 g/dl using age as a covariate.
Results & Discussion
A total of 50 Pf malaria patients ranging in age from 1.5 to 65 yr (average age 21.7 yr) were included in this study. The intensity of infection in peripheral blood of patients ranged from 319-2743 (mean = 901.29) asexual parasites/ 200 WBC. The mean fluorescence intensities (MFIs) of the expression of CD35 (mean [+ or -] SD 2.11 [+ or -] 0.43 vs 2.05 [+ or -] 0.34) and CD59 (40.65 [+ or -] SD 7.6 vs 35.16 [+ or -] 4.59) in malaria patients and healthy controls was not statistically different. On the other hand, there was a statistically significant decrease in expression of CD55 in malaria cases than controls (3.64 [+ or -] 1.88 vs 5.36 [+ or -] 1.32, P<0.01). However, the MFI of CD55 on RBCs of patients having haemoglobin level below 7 g/dl was not significantly different from malaria patients having haemoglobin level above 7 g/dl (5.5 [+ or -] 4.75 vs 6.7 [+ or -] 4.85). Waitumbi et al (3) reported decrease in expression of CR1 and CD55 on RBCs from children with severe anaemia as compared to age matched controls. However, our study did not find any correlation between expression level of RBC surface receptors CR1, CD55, CD59 and haemoglobin level in malaria patients. As in the present study Helegbe et al (10), 2007 also did not find any relationship between the severity of anaemia and levels of complement receptor 1 or decay accelerating factor (CD 55) in Ghanaian children with Pf malaria. The present study revealed that expression of delay accelerating factor (CD55) and membrane inhibitor of reactive lysis (CD59) was significantly lower in Pf cases from Assam (mean CD55 = 4.15, CD59=42.69) as compared to Pf cases from Goa (mean CD55 = 11.85, CD59=70.59) or Chennai (mean CD55 = 5.58, CD59=70.26). Interestingly, the expression of CD55 and CD59 was significantly (P<0.05) low in healthy control population from Assam (mean CD55 = 8.44, CD59=44.16) as compared to malaria cases from Goa or Chennai. This difference in expression level of RBC complement receptors may probably be due to genetic polymorphisms as has been suggested earlier (11,12). Further elaborate studies are needed to explore the pathophysiology of anaemia in malaria cases in Assam where expression of RBC complement receptors appeared to be low even in the normal healthy populations.
The first author (RCM) acknowledges the award of S.N. Bose Research Professorship. Authors thank the Director, National Institute of Malaria Research (NIMR), New Delhi for getting the Pf samples from Goa and Chennai Field Stations of NIMR.
Received March 8, 2010
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Reprint requests: Prof. R.C. Mahajan, Emeritus Professor, Department of Parasitology, Postgraduate Institute of Medical Education & Research, Chandigarh 160 012, India
R.C. Mahajan, K. Narain * & J. Mahanta *
Department of Parasitology, Postgraduate Institute of Medical Education & Research, Chandigarh & * Regional Medical Research Centre (ICMR), Dibrugarh, India
Table. Characteristics of malaria cases and healthy controls Characteristics Malaria cases Healthy controls (n=50) (n=30) Female sex (%) 16 46.7 Mean age, yr 23.4 (19.5-27.2) 23.7 (13.2-24.1) (95% CI) Mean Hb, g/dl 7.59 (7.19 to 7.99) 8.43 (7.93 to 8.93) (95% CI) Parasite density, 901.29 [micro]l (319-2743) -- mean (Range)
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|Author:||Mahajan, R.C.; Narain, K.; Mahanta, J.|
|Publication:||Indian Journal of Medical Research|
|Date:||Jun 1, 2011|
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