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An investigation of antimicrobial detergents. (Senior Division).

This experiment investigated how well antimicrobial dishwashing detergents perform. A nonpathogenic strain of Escherichia coli was grown on nutrient agar plates to serve as a stock population. This stock population of E. coli was then transferred to nutrient broth and incubated at 24 degrees Celsius for 24 hours. The E. coli broth was then diluted and mixed with varying solutions of antimicrobial detergents and water. Three different antimicrobial detergents were tested: Dawn, Sun, and Palmolive. Each of these detergents contained the antimicrobial agent triclosan. These detergent, water and E. coli solutions were then plated out on nutrient agar and incubated for 48 hours at 24 degrees Celsius. Though the results were somewhat erratic, it was obvious that all three brands of antimicrobial detergent were effective at killing the E. coli except in the very dilute trials. Other trials were performed using paper disks saturated with the detergents to identify zones of inhibition on the nutrient, agar plates with a lawn of E. coli.

Sam Huang and Andy Lin, Cherry Creek High School.
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Author:Huang, Sam; Lin, Andy
Publication:Journal of the Colorado-Wyoming Academy of Science
Article Type:Brief Article
Geographic Code:1USA
Date:Apr 1, 2002
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