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An epidemiological study of Brucellosis in rural area of North Karnataka.

Introduction

Brucellosis (undulant fever, Malta fever, or Mediterranean fever) is an important but neglected, reemerging, endemic, zoonotic, communicable disease. Although brucellosis has been eradicated in many developed countries, it still poses a serious public health issue in many developing nations, and in high endemic regions such as Africa, the Mediterranean, the Middle East, parts of Asia, and Latin America. [1] It remains a grave concern to human health in India, in particular, to the rural population who are principally engaged in agriculture, including animal husbandry, thus are in close contact with domestic animals. Alarming increase in the transmission of brucellosis in rural areas owing to the high requirement for dairy products, together with modified and exaggerated farming practices and lack of awareness among the rural population, raises concern. [2, 3]

Epidemiological evidence reveals that, in India, brucellosis is recorded in almost all states but the scenario differs between states and is present in different species of mammalian farm animals including cattle, goats, buffalo, yaks, camels, horses, and pigs. [1] Consumption of unpasteurized dairy products, contaminated food, and occupational contact are the major risks of infection to man. [4] Reports in a few countries show that contact with infected materials such as aborted fetuses, placentas, urine, manure, carcass, and salvaged animals cause human brucellosis in 60%-70% of cases. [5] In general, infection by contact is found among veterinarians, abattoir workers, farmers, animal handlers, and others who work with animals and their products. [6] The cases reported are only the "tip of an iceberg" even in endemic areas. It has been estimated that the incidence of brucellosis may be 25 times higher than the reported incidence because of misdiagnosis and underreporting. [2,7] Misdiagnosis and underreporting can happen mainly because, in many cases, patients have pyrexia of unknown origin and imitates variety of clinical entities, which makes the diagnosis tedious for an unaware physician. [5] With all such practical unresolved issues and frequent reports of suspected cases of brucellosis, we formulated an epidemiological study with a purpose to know the seroprevalence and risk factors of human brucellosis among close contacts in rural area.

Materials and Methods

This descriptive cross-sectional study was conducted between September 2013 and April 2014 at Kadoli village of Belagavi taluk and district, Karnataka, India, where predominantly the residents are involved in animal rearing and farming. Three cases of human brucellosis were reported to the Pediatric Department of the teaching hospital in August 2013. All the cases were aged younger than 15 years. Serological studies revealed a significant high titer (20480 lU/mL) by a standard agglutination test (SAT), which was diagnostic of brucellosis and further confirmed by blood culture. Therefore, occupationally exposed individuals with or without pyrexia of unknown origin (PUO) and suspected contacts of the cases were identified in the surrounding areas and included in the study using purposive sampling.

The purpose of the study was explained to the study subjects who were the permanent residents of Kadoli village, and informed consent was obtained from the participants. Data were collected by using a predesigned and structured questionnaire, which included the sociodemographic details and behavioral risk factors. This was followed by a physical examination of all the contacts by the investigators. Among the subjects who were presumptively diagnosed as brucellosis contacts, serum samples were collected after obtaining a written informed consent.

Serum samples were first screened by Rose Bengal plate test (RBPT) and further subjected to SAT for titers at Microbiology Department of teaching hospital. Cases were defined by the presence of a Brucella agglutination titer of at least 160 lU/mL or more, if the personnel had signs or symptoms compatible with brucellosis according to the recommended case definition criteria suggested by the World Health Organization (WHO). [5,8] The serum samples were further tested with 2-mercaptoethanol (2-ME) to confirm acute brucellosis infection. [9, 10]

Data were expressed as percentages, and statistical analysis was done using [chi square] test using Statistical Package for Social Sciences (SPSS, Inc., version 17.0). P-value <0.05 was considered as statistically significant.

All seropositive subjects were provided with a course of doxycycline treatment (100 mg twice daily) for 6 weeks. Serum samples of 48 goats, from all the houses of the contacts with possible exposure, were also subjected to RBPT. Health education lectures were organized to create awareness among the high-risk groups (farmers, shepherds, and animal handlers) regarding brucellosis involving the Community Medicine Department, Gram Panchayat, veterinary hospital, and Primary Health Center, Kadoli, Karnataka, India.

Result

Of the total 190 contacts of the cases, 104 (54.7%) were men and 86 (45.3%) women. The predominant occupation of the male participants was livestock handling (47.2%) and of female participants was household work (36.0%). This difference was found to be statistically significant (P < 0.0001) [Table 1]. The mean age of the participants was 24.57 [+ or -] 15.41 years. Majority were in the age group of 16 to 30 years (35.8%) [Figure 1]. Among the study participants, 47.4% of them showed signs and symptoms suggestive of brucellosis and were presumptively diagnosed as brucellosis, of which predominantly were in the age group of 1-15 years (66.6%) [Table 2].

The serum samples of all the persons who were clinically suspected was collected and screened by RBPT, which showed positivity in 42.2% of them. The seroprevalence of brucellosis was found to be 28.8% among clinical suspects using SAT. About 15.4% of them showed positive result with 2-ME blocking agent, indicating acute infection [Table 3 and Figure 2]. The behavioral risk factors of the study participants were assessed; majority were animal handlers (42.6%), and 31.1% of them gave history of consuming unpasteurized raw milk [Table 4].

Substantial evidences revealed possibility of high prevalence of infection among the goats reared by the family and whose milk was regularly consumed by them and others in the village without any pasteurization. Among the 48 goats tested, 29.2%of goats showed the evidence of Brucella infection, which was confirmed by the Institute of Animal Health and Veterinary Biologicals, Belagavi, Karnataka, India.

Discussion

In our study, a serious threat of an outbreak was identified soon after serial reporting of cases of brucellosis from Kadoli village. All the three index cases were in the age group of younger than 15 years, suggesting lower immunity toward the infection. Children can be particularly at risk as they may adopt new born or sick animals as pet. [5]

Brucellosis is endemic in the Indian subcontinent as it is reported in almost all states. The rearing of domestic cattle and unsafe animal handling practices has been suggested as an important factor in the maintenance and spread of infection. [1, 11] The prevalence of human brucellosis reported in the previous studies in Andhra Pradesh and Orissa was found to be 11.5% and 6.5%, respectively. [12, 13] In a similar seroprevalence study done at Ludhiana, Punjab, revealed that 24.5% of the participants were positive by RBPT, and diagnosis was established in 26.6% using SAT with a titer range between 80 and 1,280 IU/mL. This result was comparable with our study; however, this study had a lower cut off value of 80 IU/mL for establishing diagnosis. Lack of knowledge about zoonosis, contact with parturient animal, and raising animals were recorded as the major causative factors. [14] The findings were found to be consistent with our study, except for the fact that the knowledge regarding the disease was not included in our study.

In a study done by Mathur et al., [15] the seroprevalence of brucellosis among dairy personnel in contact with infected animal was 8.5%. In another study by Thakur and Thapliyal, [16] a prevalence of 4.97% in animal contacts and 17.39% among veterinarians was reported. [16] A study done in Gujarat showed SAT positivity 8.5%. [17] On the contrary, the seroprevalence noted in our study was considerably higher when compared, indicating a higher infection rate.

A study by Mantur et al. reported that, of the 5,726 study subjects, 93 children with brucellosis were identified by testing samples with seroprevalence of 1.6% by SAT (>160 IU/mL), and the diagnosis was confirmed in 43 of these pediatric patients by the isolation of Brucella melitensis. Majority of the pediatric patients and their family members were shepherds (41.9%), and the most probable risk factors responsible for infection were practice of consuming fresh goat milk and the close contacts with animals. The most important fact was that brucellosis was alleged on first analysis only in 15 cases; however, in the rest 78 cases, initial identifications were as enteric fever, malaria, PUO, and rheumatic arthritis. [18] Although the prevalence was very low, the positivity among pediatric cases were comparable with our study. Thus, the children are at most risk of developing Brucella infection.

Majority of the patients in our study were shepherds from rural area, and because of regular close contact with animals when taking them to the fields and unsafe handling of infected products of gestation during parturition, high seroprevalence has been reported. In the study done by Kochar et al. [19] from Northwest India reported a similar finding, with an additional information on the clinical presentation of the cases and their diet pattern, which included consumption of raw milk of cattle and goats, which is heavily infected with the organism. [19]

Similarly, Almuneef et al. [20] emphasized in their study the significance of screening household members and contacts of acute brucellosis cases in endemic areas. This is an important epidemiological step that must be taken into account by the family physicians, so that timely diagnosis and provision of therapy can result in lower morbidity. Brucellosis must be more importantly reported to health authorities, and awareness of the infection occurrence can be used to prioritize disease control policy for brucellosis and to alert health staff. [21]

Limitations

Sample size could not be calculated as there was a risk of impending outbreak; hence, the contacts were selected by purposive sampling. The serological examination of asymptomatic contacts and culture for isolation of species of Brucella for the symptomatic contacts could not be done because of operational and resource constraints.

Conclusion

Our study demonstrated a significant seroprevalence of brucellosis in the rural area of North Karnataka, among the contacts who are often neglected or misdiagnosed. The risk of developing brucellosis was attributed to poor animal handling practices and consumption of unpasteurized milk. Apparently low incidence is an illusion, as many cases go unreported. Hence, it is recommended that brucellosis must be included in public health education, particularly in the rural areas, as it imposes a dual burden on human and animal health. High index of suspicion is needed for early diagnosis and prompt treatment. Regular examination is essential to monitor the presence or absence of human/animal brucellosis and formulate strategies for intervention.

DOI: 10.5455/ijmsph.2015.20032015248

Acknowledgment

We thank the staff of Primary Health Centre, Kadoli, Institute of Animal Husbandry and Veterinary Biologicals, Belagavi, and the members of Gram Panchayat, Kadoli village for their co-operation in the conduct of the study. We acknowledge the support of Dr. Vijaya A. Naik, Former Professor and Head of Community Medicine, Jawaharlal Nehru Medical College, KLE University, Belagavi, Karnataka, India.

References

[1.] Renukaradhya GJ, Isloor S, Rajasekhar M. Epidemiology, zoonotic aspects, vaccination and control/eradication of brucellosis in India. Vet Microbiol 2002; 90(1-4):183-95.

[2.] Mantur BG, Amarnath SK. Brucellosis in India--a review. J Biosci 2008; 33(4):539-47.

[3.] Smits HL, Kadri SM. Brucellosis in India: a deceptive infectious disease. Indian J Med Res 2005; 122:375-84.

[4.] Godfroid J, Cloeckaert A, Liautard JP, Kohler S, Fretin D, Walravens K, et al. From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Vet Res 2005; 36:313-26.

[5.] Brucellosis in Humans and Animals. Geneva: World Health Organization in collaboration with the Food and Agriculture Organization of the United Nations and World Organization for Animal Health, 2006.

[6.] Joint FAO/WHO Expert Committee on Brucellosis. 6th Report. Geneva: World Health Organization, 1986.

[7.] Ministry of Health and Family Welfare, Government of India. Bulletin on Rural Health Statistics. 2007. Available at: http://mohfw.nic.in/BuNetm%20on%20RHS%20%20March,%20 2007%20%20PDF%20Version/Title%20Page.htm (last accessed on September 19, 2014).

[8.] Alton GG, Jones LM, Pietz DE. Laboratory techniques in brucellosis. Monogr Ser World Health Organ 1975;55:1-163.

[9.] Buchanan TM, Faber LC. 2-mercaptoethanol Brucella agglutination test: usefulness for predicting recovery from Brucellosis. J Clin Microbiol 1980; 11(6):691-3.

[10.] Mantur BG, Amarnath SK, Shinde RS. Review of clinical and laboratory features of human brucellosis. Indian J Med Microbiol 2007; 25(3):188-202.

[11.] Sehgal S, Bhatia R. Zoonoses in India. J Commun Dis 1990; 22(4):227-35.

[12.] Mrunalini N, Reddy MS, Ramasastry P, Rao MR. Seroepidemiology of human brucellosis in Andhra Pradesh. Indian Vet J 2004; 81(7):744-7.

[13.] Mohanty TN, Panda SN, Das BR, Pradhan SK, Pradhan RK. Sero-incidence of brucellosis among dairy farm workers in Orissa. Indian Vet J 2000;77(7):568-70.

[14.] Yohannes M, Gill JPS. Sero-epidemiological survey of human brucellosis in and around Ludhiana, India. Emerg Health Threats J 2011;4:7361.

[15.] Mathur TN. Brucella strains isolated from cows, buffaloes, goats, sheep and human beings at Karnal. Their significance with regard to epidemiology of Brucellosis. Indian J Med Res 1964;52:1231-40.

[16.] Thakur SD, Thapliyal DC. Seroprevalence of brucellosis in man. J Commun Dis 2002;34(2):106-9.

[17.] Panjarathinam R, Jhala CI. Brucellosis in Gujarat state. Indian J Pathol Microbiol 1986;29:53-60.

[18.] Mantur BG, Akki AS, Mangalgi SS, Patil SV, Gobbur RH, Peerapur BV. Childhood brucellosis--a microbiological, epidemiological and clinical study. J Trop Pediatr 2004;50(3):153-7.

[19.] Kochar DK, Gupta BK, Gupta A, Kalla A, Nayak KC, Purohit SK. Hospital-based case series of 175 cases of serologically confirmed brucellosis in Bikaner. J Assoc Physicians India 2007; 55:271-5.

[20.] Almuneef MA, Memish ZA, Balkhy HH, Alotaibi B, Algoda S, Abbas M, et al. Importance of screening household members of acute brucellosis cases in endemic areas. Epidemiol Infect 2004;132(3):533-40.

[21.] Kumar A. Brucellosis: need of public health intervention in rural India. Sec biol Med Sci 2010;31(1):219-31.

Source of Support: Nil, Conflict of Interest: None declared.

Avinash Kavi (1), Shivaswamy M Shivamallappa (1), Sharada C Metgud (2), Vishwanath D Patil (3)

(1) Department of Community Medicine, Jawaharlal Nehru Medical College, KLE University, Belagavi, Karnataka, India.

(2) Department of Microbiology, Jawaharlal Nehru Medical College, KLE University, Belagavi, Karnataka, India.

(3) Department of Pediatrics, Jawaharlal Nehru Medical College, KLE University, Belagavi, Karnataka, India.

Correspondence to: Avinash Kavi, E-mail: dravinashkavi@gmail.com

Received March 20, 2015. Accepted April 3, 2015

Table 1: Distribution of the study participants according to
their gender and occupation (N = 190)

Predominant occupation     Men (%)      Women (%)    Total

Farmers                    38 (36.5)    14 (16.3)    52 (27.4)
Livestock handlers         49 (47.2)    29 (33.7)    78 (41.0)
  (shepherds)
Household contacts         7 (6.7)      31 (36.0)    38 (20.0)
Students                   10 (9.6)     12 (14.0)    22 (11.6)
Total                      104 (100)    86 (100)     190 (100)

[chi square] = 30.109; df = 3 P < 0.00001.

Table 2: Percentage of subjects who were positive for symptoms
suggestive of brucellosis (N = 190)

Age group (years)    Total No. of persons     No. of persons with
                     examined (%)             symptoms S/O
                                              brucellosis (%)

0-15                 51 (26.8)                34 (66.6)
16-30                68 (35.8)                28 (41.2)
31-45                38 (20.0)                18 (47.3)
46-60                27 (14.2)                8 (29.6)
61-75                6 (3.2)                  2 (33.3)
Total                190 (100)                90 (47.4)

Table 3: Percentage of contacts who were positive for
serological tests for Brucella antigen (N = 90)

Age group (years)    No. of persons       No. of positive for
                     with symptoms        screening RBPT (%)

0-15                 34                   12 (35.3)
16-30                28                   14 (50.0)
31-45                18                   8 (44.4)
46-60                8                    2 (25.0)
61 and older         2                    2 (100.0)
Total                90                   38 (42.2)

Age group (years)    SAT titer a 160      No. of Positive for
                     IU/mL (%)            STAT + 2-ME (%)

0-15                 6 (17.6)             4 (11.8)
16-30                12 (42.8)            2 (7.1)
31-45                4 (22.2)             4 (22.2)
46-60                2 (25.0)             2 (25.0)
61 and older         2 (100.0)            2 (100.0)
Total                26 (28.8)            14 (15.4)

Table 4: Behavioral risk factors of brucellosis among the study
participants (N = 190)

Behavioral risk factors                    No. of         Percentage
                                           participants

History of consuming raw milk              59             31.1
Unsafe animal handling practice            81             42.6
History of food contamination              38             20.0
Consumption of undercooked meat            12             6.3
Total                                      190            100

Figure 2: Percentage of people with different age group and
percentage who were infected (positive for SAT
[greater than or equal to] 160 IU/mL).

Age group in years   Non-infected  Infected
0-15                 64.7          35.3
16-30                50            50
31-45                55.6          44.4
46-60                75            25
61-75                100

Note: Table made from bar graph.
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Title Annotation:Research Article; Karnataka, India
Author:Kavi, Avinash; Shivamallappa, Shivaswamy M.; Metgud, Sharada C.; Patil, Vishwanath D.
Publication:International Journal of Medical Science and Public Health
Article Type:Report
Date:Sep 1, 2015
Words:2855
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