An association of hepatitis virus infection with rare hemopoietic malignancies.
High prevalence of HBV in leukemia cases and HCV with ALL, NHL& AML have been reported in several case series (2,3). However association of HBV with RAEB, CML &AML are reported in very few cases. (4,5,6,7)
Data substantiating association of hematopoietic malignancies with viral hepatitis is limited more so with subtypes. One case of AML-M7, a rare entity, was HBsAg positive in our series.
METHOD: Present study was conducted from 2009 to 2012 in Department of Pathology, Gandhi medical college, Bhopal, Madhya Pradesh to assess the relationship of HBV with hemopoietic malignancies, out of 63 hematopoietic malignancies/premalignant conditions diagnosed on bone marrow examination, 5 cases were found to be HBsAg positive by ELISA.
RESULTS: Out of 5 cases, 3 were males & 2 females; 1 was a child (12 years) and 2 adult (22 and 38 years) & 2 elderly (50 & 55 years) respectively. All were negative for HCV. Hepatosplenomegaly was present in 2, anemia in all and leucopenia in 3 cases. Platelets counts were normal in 1, decreased in 3 and increased in 1 case. Pancytopenia was present in 3 of 5 cases. Blasts were increased in 2 cases (in the range of AML). Cytoplasmic blebbing and vacoulation, nuclear clefting and platelet budding were seen in case 4 (figure 1). Auer rods were present in case 1. (figure 2) Bone marrow examination confirmed the diagnosis of AML in 2 (one of which was AML-M7), RAEB in 1 and aplastic anemia in 2 cases. Here we have included Aplastic Anemia and RAEB because it may evolve 15% and 25 % into leukemia respective.
Clinico hematological profile of five HBsAg positive patients is following:
Features Case I Case II Case III 1. Age/Sex 50 yrs/M 12 Yrs/F 55 yrs/F 2. Clinical features Liver Pain Chronic liver cirrhosis, abdomen, disease with Portal Nausea, ascites. hypertension headache History of With ascitis jaundice 1 year back 3. HBsAg positive + + + 4. HCV - - - 4. Hepatosplenomegaly - + - 5. Hb gm/dl 8.0 11.2 7.2 6. TLC cells/cumm 3,100 8600 2,500 7. Platelet count/cumm 50,000 1,80,000 Less than 10,000 8. Pancytopenia + - + 9. Blasts 22 08 Nil 10. Morphology of blasts a. Cytoplasmic - - - Blebbing b. Cytoplasmic - - - vacuolation c. Nuclear Clefting - - - d. Platelet budding - - - e. Auer rods + - - 11. Bone Marrow and Blasts 35% Blasts 14% Aplastic anemia Final Diagnosis AML RAEB Features Case IV Case V 1. Age/Sex 22Yrs/M 38yrs/M 2. Clinical features Generalized Bleeding gums, weakness, Weakness, melena breathlessness 3. HBsAg positive + + 4. HCV - - 4. Hepatosplenomegaly - + 5. Hb gm/dl 3.7 6 6. TLC cells/cumm 4,500 1,500 7. Platelet count/cumm 9,00,000 20,000 8. Pancytopenia - + 9. Blasts 35 Nil 10. Morphology of - blasts a. Cytoplasmic + - Blebbing b. Cytoplasmic + - vacuolation c. Nuclear Clefting + - d. Platelet budding + - e. Auer rods - - 11. Bone Marrow and Blasts 55% Aplastic anemia Final Diagnosis AML M7
DISCUSSION: Induction of cancers by exogenous agents such as chemical, radiation and viruses have been interesting area of research.
Data of hematopoietic malignancies especially many subtypes associated with viral hepatitis is very limited.
HTLV, HCV, HBV have been assessed in relation to hemopoietic malignancies.
We have also used only one marker i.e. HBsAg to ascertain HBV infection.
Out of HBV markers like HBsAg, anti-HBsAg, anti-HBcAg, HBV, in 50 patient series, at least one marker was positive at initial diagnosis of leukemia in 68% of AML and 71% of ALL patients (4). HBsAg was positive in 12% of AML & 25 % OF ALL patients. In remission HBsAg was positive in 83% of AML and 44% of ALL patients.HBVDNA in bone marrow of AML and CML patients were found significantly increased in leukemic than normal patients (6). However association of HBV with other subtypes of leukemia is uncertain. (6,8) There is non-significant excess of HBsAg with RAEB and anti HBcAg with AML. (5)
In 15% of leukemia-HBV DNA in bone marrow cells (8 out of 51)were positive .Out of these 8 one was serum HBsAg positive (7). 4 other patients developed antibody to HBV, but were negative for HBV DNA at the time of initial diagnosis. HBV DNA was positive in bone marrow in 55% of myeloid leukemia & 7% of lymphoid leukemia at the time of diagnosis. (7)
HBV infection was significantly associated with earlier precipitation of Diffuse large B cell lymphoma, extra nodal marginal zone B cell lymphoma, peripheral T cell lymphoma and B cell lymphoma. (2)
HBV was more common than HCV in the pathogenesis of hematological malignancies in Korea. (9)
Prevalence of positivity for HBsAg was 8.5% among B-NHL in contrast to 2.8 % in control (3). Prevalence of HBV was 7.3% in NHL which was higher than in Acute Leukemia (1.7%) in contrast to the general Japanese population (1.2%). (10)
HBV reactivation is common following chemotherapy and it is associated with high mortality despite prompt antiviral treatment .Increased risk for HBV reactivation was seen more in younger males with high viral load with HBsAg /HBeAg positive lymphomas and pre-immune suppression with HBV-DNA .Hematological malignancies reported in association with hepatitis B virus reactivation are -AMLK, ALL, CML, CLL, NHL, Hodgkin's Disease, Multiple myeloma, Plasma cell dyscrasias, MDS and Aplastic anaemia. (1)
In our series of 63 patients, 2 out of 4 Aplastic anemia (50%), 1 out of 3 RAEB cases (33.3%) and 2 out of 24 AML cases (8.33%, one of which was AML M7) were HbsAg positive (5 in all).
Amongst newly diagnosed cases of acute leukemias, anti HCV was found in 1.28% cases. After 1 yr of diagnosis of HCV positivity incidence rose upto 67 % in AML and 40%in ALL. (4)
Lesley A. Anderson, Ruth Pfeiffer, et al (2008) have made a very interesting remark that HCV having increased risk for AML & NHL(diffuse large B-cell lymphoma, Burkitt's lymphoma, Follicular lymphoma& marginal zone lymphoma.) HCV may induce lympho proliferative malignancies through chronic immune stimulation. (8)Prevalence of HCV is found in 10.1% in NHL cases, 2.9% in acute leukemias as compared with general population in Japan which was 2.6%. (10)
HCV plays a significant role in the etiology of chronic hepatitis in leukemic patients and that persistent anti-HCV activity correlates with a more severe chronic liver disease which could jeopardize the final prognosis of children cured of leukemia. (11).
These findings stress the need for identification of hepatitis virus in patients of hematopoietic malignancies.
CONCLUSION: Worldwide prevalence of HBV is more in malignant than in general population. Infection associated with a significantly earlier disease onset suggestive of possibility of HBV playing an etiological role in the induction of B-cell NHL. Overt and occult HBV pose a serious but preventable threat.HBV infection has potentially significant influence and play a major role in the process of myeloid malignancy development. However, number of cases are not big enough to draw a firm conclusion, further studies with large population of leukemic patients are needed to confirm the possibility of such an association.
Screening for HBV, HCV infection in all haemato-oncology patients pre and post chemotherapy should be practiced by all clinicians who are treating such patients.
HBV negative patients should be immunized against HBV
I would like to extend special acknowledgement to Dr. U.M. Sharma, Dr. Udayan Vajpeyi, Mr. Atul Shrivastava, Dr. Sharda Balani and technical staff Mr. K.P. Verma for their support.
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R.K. Nigam , Rubal Jain , Reeni Malik , Neha Banseria , Rajnikant Ahirwar 
[1.] R.K. Nigam
[2.] Rubal Jain
[3.] Reeni Malik
[4.] Neha Banseria
[5.] Rajnikant Ahirwar
PARTICULARS OF CONTRIBUTORS:
[1.] Professor, Department of Pathology, Gandhi Medical College, Bho pal, Madhya Pradesh.
[2.] PG Student, Department of Pathology, Gandhi Medical College, Bho pal, Madhya Pradesh.
[3.] HOD, Department of Pathology, Gandhi Medical College, Bho pal, Madhya Pradesh.
[4.] PG Student, Department of Pathology, Gandhi Medical College, Bho pal, Madhya Pradesh.
[5.] PG Student, Department of Pathology, Gandhi Medical College, Bho pal, Madhya Pradesh.
NAME ADDRESS EMAIL ID OF THE CORRESPONDING AUTHOR:
Dr. Rajendra Ku mar Nigam, C-116, Shahpura, Bhopal, Madhya Pradesh, India--46209.
Date of Submission: 19/09/2013.
Date of Peer Review: 21/09/2013.
Date of Acceptance: 23/10/2013.
Date of Publishing: 25/10/2013
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|Title Annotation:||CASE REPORT|
|Author:||Nigam, R.K.; Jain, Rubal; Malik, Reeni; Banseria, Neha; Ahirwar, Rajnikant|
|Publication:||Journal of Evolution of Medical and Dental Sciences|
|Date:||Oct 28, 2013|
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