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An assessment of heavy metal toxicity in embryonic and larval axolotl (Ambystoma mexicanum).

Embryos and 75 day old larvae of the model organism Ambystoma mexicanum were treated with differing concentrations of the heavy metals Cadmium (Cd[Cl.sub.2]) and Methyl mercury (HgC[H.sub.3]). Each experiment exposed embryos and larvae to 8 treatment levels including controls. Treatment levels for cadmium were 0ug/L, 15ug/1, 50ug/1, 150ug/l, 1500ug/1, 15,000ug/l, 150,000ug/l and 1.5million ug/1. Treatment levels for methyl mercury were 0ug/l, 1.5ug/1, 5ug/l, 15ug/l, 50ug/l, 150ug/l, 1500ug/l and 15,000ug/l. All treatment groups were observed every 12hrs during the 96hr treatment for mortality, physical anomalies and behavioral anomalies. Tissue samples were collected from deceased individuals and organisms were fixed in 10% formalin and preserved in 70% ethanol for future analysis. Embryonic LC-50 values for cadmium are shown in Figure 1. Embryos were scored for development (figure 2.) according to Bordzilovskaya et al (1).

[FIGURE 1 OMITTED]

[FIGURE 2 OMITTED]

We present the embryonic and larval LC-50 values as well as developmental staging and percent survivorship. Toxicologically, cadmium LC-50 concentrations were higher than mercury. Additionally, mercury may have a greater effect on survival beyond exposure period at lower ecologically relevant doses.

1) Bordzilovskaya, N.P., T.A. Dettlaff, Susan T. Duhon, and George M. Malacinski. 1989. Developmental-stage series of axolotl embryos. In Developmental Biology of the Axolotl edited by J.B. Armstrong and G.M. Malacinski. Oxford University Press, New York, pp. 201-219.

Kenneth C. Cabarle (1,2) and Christopher K. Beachy (2)

(1) Department of Biology, University of North Dakota, Grand Forks, ND 5, USA; (2) Department of Biology & Amphibian Growth Project, Minot State University, Minot, ND 58707, USA
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Title Annotation:COMMUNICATIONS-GRADUATE
Author:Cabarle, Kenneth C.; Beachy, Christopher K.
Publication:Proceedings of the North Dakota Academy of Science
Article Type:Report
Geographic Code:1USA
Date:Apr 1, 2008
Words:285
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