Printer Friendly

ASSESSMENT OF POTENTIAL OF PREDATORY SPIDERS IN CONTROLLING THE COTTON JASSID (AMRASCA DEVASTANS) UNDER LABORATORY CONDITIONS.

Byline: R. Khuhro, A. Ghafoor, A. Mahmood, M. S. Khan, S. Andleeb, M. Bukhari, I. Maqsood, M. M. Shahjahan and N. A. Baloch

ABSTRACT

A laboratory experiment was conducted to evaluate the predatory efficiency of aranied fauna against the insect pests of cotton. Predatory spider species collected from cotton fields were Lycosa tista, L. kempi, L. machenziei and Pardosa berminica and Thomisus projectus, T. bulani and Thomisus sp and their preys were adult and nymphs of jassid, white flies and jassid. Predator L. tista consumed 12.80 (19.21) adult and 17.0 (21.25%) nymphs jassid out of total consumption of adult jassid (66.60) and nymph jassid (79.7) whereas L. kempi consumed 12.80 (19.21%) white flies and 10.60 (25.80%) thrips. Predatory spider T. projectus showed least intention for consumption of 6.0 (9.0%) adults and 6.40 (8.0%) nymph jassid. White flies and thrips were consumed 3.80 (5.01%) and 3.20 7.61% by the T. bulani and T. projectus. The maximum nymph jassid (79.40) were consumed by all predatory spiders. Overall the highest consumption was observed in L. kempi (56) followed by P. birminica (53) and Thomisus sp (47).

The current findings revealed that aranied fauna as an efficient predator of jassid could be used for the suppression of insect pests of cotton.

Keywords: spiders, predation, insects, pests, cotton, consumption.

INTRODUCTION

Cotton is an important cash crop of Pakistan, plays a pivoted role in the economy of the countries. It is grown almost all over the world from times immemorial and perhaps earlier that the time when Peruvian mummies were clothed in it. It remained a luxurious plant in China upto 600 A. D., later introduced by the Muslim Caliphs of Cordova in Spain and then it was taken to Europe about a thousand years ago (Khoso, 1994). Farmers have been growing cotton since 4,000 B.C. in India. In the new world, cotton production goes back well before Columbus landed in the Bahamas in 1942 (Dumka et al. 2004). Sindh is the second largest cotton producing province of Pakistan with a total area of 635 thousand hectares with annual production of 300 thousand bales. However, the seed cotton yield per hectare obtained in the country is 769 kg ha-1, while in Sindh province it is 804 kg ha-1. Per hectare yield obtained in the province is greater as compared to other three provinces of Pakistan (Anonymous, 2006).

Cotton is attacked by number of pest species and suffers heavy losses. The yield of cotton crop in Pakistan is very low, 622 kg ha-1 (Channa et al. 2006). Traditional pest control through pesticides has disturbed the ecosystem and killed the natural enemies of the pests. Secondly, due to excessive use of pesticides, the pests have developed resistance against many pesticides. Keeping the above scenario in view, the work on-IPM techniques is being done all over the world including Pakistan.

Spiders are effective biological controlling agents, prey on several pests in agro-ecosystem (Hodge, 1999). Spider guilds having different ecological niches may collectively play an important role in suppressing the populations of insect pests (Ghafoor, 2002). Spiders are one of the most abundant predatory groups in the terrestrial ecosystems. They feed on insects, some other arthropods and play important role in pest control. 3500 species of spiders have been identified in the world (Ghavami et al. 2007). They are carnivorous arthropods, consume a large number of preys and do not damage plants. They have unique habitat and they live in almost all the environments. Spiders serve as buffers that limit the initial exponential growth of prey populations. The predatory spiders are classified into five major groups based on their foraging style.

Prey searching ability, wide host range, ease in multiplication and poly-phagous in nature make them as a potential predator in biological pest suppression (Rajeswaran et al. 2005). In present experiment, the population of predatory spiders on cotton pests was carried out for first time in Sindh, Pakistan. The identification of spiders and their feeding potential on sucking insect pests will be helpful for the management of pests on cotton crop in future.

MATERIALS AND METHODS

Insect pests and aranied fauna were collected from fields of cotton variety Shahkar. The specimens were brought to the laboratory of Plant Protection Department for identification purpose. Predators spiders belonged to Family Lycosidae were Lysosa tista, L. kempi, L. machenziei and Pardosa berminica while thomisidae consisted of Thomisus projectus, T. bulani and Thomisus sp. The insect pest of cotton used for predation purpose was adult and nymphs of jassid, white flies and thrips. Hand picking and nets were used for the capture of preys and predators. Both types of materials were brout to laboratory and identified by using the relevant literature (Diyal, 1937; Tikader, 1982). Collected specimens were of the same age and sex. Each of the five insects pests were introduced to each predator in a separate glass Petri-plate (6.0 cm, dia) Few pieces of chopped cotton leaves were put for study of their proper feeding and consumption behavior.

A total of twenty five insect pests were introduced to each individual predator species. After twenty four hours their consumptions were observed. Each treatment was repeated by five times. Average temperature and relative humidity (%R.H.) during whole experiment remained 30+-2oC and 76+-5.35% respectively. Collected data were subjected to analysis of variance (ANOVA) (Steel et al., 1997) and their means were compared using Duncan's new Multiple range test (Duncan, 1955).

RESULTS

Lysosa tista consumed 12.80 adult jassid which was 19.21% out of total consumption followed by L. kempi (9.80) and Thomesus sp (9.60). Overall mean consumption was 11.20+-1.20. Maximum consumption of nymph jassid (17.00) was also performed by L. tista. It was 21.25% of the total consumption by all predatory spider species (p less than 0.01). Pardosa birmanica consumed 14.40 (17.5%) and 12.80 (15%). The predator L. kempi gathered white flies 12.80 (p less than 0.01) followed by 12.60 Thomesus sp (19.35) and 11.20 L. tista (17.7%). The insect pest 10.60 thrips (25.80%) (p less than 0.01) became the part of food of L. kempi, 10.0 of L. machenzeie (24.80%) and 3.20 of T. projectus (7.61%). The least 6.0 (9.00%) adult jassid, 6.40 (8.0%) nymph jassid (p less than 0.01) and 3.20 (7.61%) thrips were consumed by T. projectus.

Overall among all araneid fauna least 3.80 (5.01%) of white flies were preyed by T. bulani (p greater than 0.01) whereas L. kempi (11.20+-2.30) performed maximum mean consumption followed by L. tista (11.20+-1.20) and P. berminica (10.80+-0.22). Among all preys maximum consumption was observed for nymph jassid (79.4) followed by adult jassid (66.6) and white flies (63.6) while least consumption of thrips (43.8). Maximum introduced insect pest were consumed by L. kempi (56) followed by P. berminica (54) and Thomesus sp (47) (p less than 0.01) whereas least trend for consuming insect pests observed in T. projectus (26.5) (p greater than 0.01).

Table 1. Data (mean+-SD) for the consumption of insect pests of cotton by different predatory Araneid fauna

###Mean number of insect pests consumed

Spiders species###Adult jassid###Nymph jassid###Whitefly###Thrips###Mean

L. tista###12.80a###17.00c###11.20ab###3.80b###11.20+-1.20a

L. kempi###9.80b###11.60c###12.80a###10.60a###11.20+-2.30a

L. mackenziei###7.80cd###8.40de###9.60bc###10.00a###8.95+-2.44cd

Thomisus sp. nov.###9.60bc###12.80bc###12.60b###2.60b###9.40+-1.88b

P. birmanica###11.80a###14.40b###8.00cd###9.00a###10.80+-0.22ab

T. projectus###6.00d###6.40e###5.60de###3.20b###5.30+-0.44f

T. bulani###8.80bc###8.80d###3.80e###4.60b###6.50+-0.33e

Similar alphabets in columns and rows for respective cotton pests did not differ significantly (p less than 0.01)

Table 2. Analysis of variance (ANOVA) for consumption of insect pests of cotton by different predatory spider species.

SOV###df###Mean sum of squares

###Adult jassid###Nymp jassid###White fly###Thrips

Predators###6###26.590###69.247###60.123###59.914

Preys###5###20.45###51.87ns###72.65ns###67.42ns

Predators X Preys 10###5.98###54.38ns###36.98###31.98

Errors###13###0.971###1.08###2.500###1.257

Total###34###27.561###175.33###172.3###158.25

C.V. (%)###4.48###4.86###17.40###17.92

Statistically significant differences (p less than 0.01); NS, non-significant differences (p greater than 0.01).

The predators L. kempi (14.71%) and (14.41%) Thomesus sp consumed about similar number of adult jassid likewise L. machenziei (10.0%) and T. bulani (10.0%) preferred nymph jassid as a whole. Similarly L. kempi and (20.3%) and Thomisus sp (19.35%) showed their same intentions for predation on whitefly of cotton. Both the species L. kempi (25.80%) and L. machenziei (24.85%) of genus Lycosa found attracted towards thrips as regarding the total consumption of thrips (43.8). From the results it was revealed that most of the araneid fauna preferred predation on nymph jassid (79.4) followed by adult jassid (66.6) applied prey species.

DISCUSSION

Present study on population of predatory spiders on cotton pests at Tandojam revealed that seven species of predatory spiders were identified for first time in Sindh. The information on the number of predatory spider species showed that there is potential for knowing number of predators in various crops, fruits and vegetable plants. The studies showed that the predators were polyphagous (preys) in nature and can be exploite for biological control of sucking pests. Better adaptations of body parts of spider proved them better predators for their preys. Spiders are very effective biological control agents and preyed on several pests in numerous agro- ecosystem (Hodge, 1999). Spider guilds having different ecological niches may collectively play an important role in suppressing the populations of insect pests (Ghafoor, 2002). Spiders are one of the most widely occurring and frequent predatory groups in the land ecosystems.

The study also showed that there were only two types of predatory spider viz., crab and wolf spider prevailing in cotton agro-ecosystem at Tandojam. Current species of spiders proved better controlling of insect pests of cotton, specially, preferred nymph jassid than adults. predation on adults might be difficult in handling as compared to nymphs. Most of the spider species consumed more specimens of nymph jassid than other preys. Slow movement and less resistance offered great advantages to predators.

Role of spiders for biological control of insect pests of cotton were studied by many researchers (Ghafoor, 2002; Ghafoor et al., 2011; Alvi, 2007, Maqsood, 2011; Rajeswaran et al., 2005; Gustavo and Joas, 3003). Sebastian and Sudhikumar (2003) evaluated feeding potential of fourteen predatory spider species of cotton in laboratory on Aphis craccivora infesting cotton. The feeding efficiency varied with the life stage and sex of the spiders. Adult females consumed more number of preys than adult males and sub-adults. Among all species tested, Lysosa poonaensis and L. tista consumed the highest number of prey and Oxyopes chitrae and Phidippus pateli consumed the least number of preys. Least consumption might be due to the more adaptability and mimic behavior of pest against the predators. Similarly in the current studies L. tista consumed 12.80 adult jassid (19.21%) and overall its consumption was 44.8 insect pests.

Further studies revealed the fruitful results of using spiders as a best biological controlling tool for insect pests of cotton. Predatory spider species can used to reduce in infestation of insect pests of crops, fruits and vegetables in order to reduce the huge economic losses and economic inflations. In such laboratory conditions spiders proved best enemy for reducing the insect pests of cotton. In current experiments L. tista consumed maximum 12.80 (19.21%) adult jassid and 17.00 (21.25%) nymph jassid among all pests introduced. It might be due to their special body part and special characteristics for capturing ability for their preys and therefore, consumed more preys than other araneid fauna. Those results were in accordance with conclusive remarks of Sebastian and Sudhikumar (2003) who concluded that L. tista consumed the highest number of preys whereas Oxyopes chitrae and Phidippus pateli least.

Present investigation showed that all predatory spiders consumed at least on of their prey pests at controlled laboratory conditions. However, some spider species consumed more efficiently than others competitive. The current studies can be exploited by using the pest control in field conditions by releasing the predators and pests in controlled conditions/cages. The studies may be included on IPM of pests so that adverse effects on pesticides may be excluded for the coming generations. It was proved from the studies that at the start of jassid development and growth the release of predators might be more advantageous than at the adult stages.

The spider L. tista consumed maximum of 12.80 (19.21%) adult jassid and 17.0 (21.25%) nymph jassid among all introduced insect pests. The species L. kempi (56) showed maximum intention for consumption of cotton pests. It might be due to their best adaptations of body part for hunting than other araneid fauna. The role of predatory spider species for the consumption of insect pests of cotton were in agreement with those of Rajeswaran et al. (2005) who reported that spiders are carnivorous and poly-phagous arthropods consume a large number of preys and do not damage plants. El- Heneidy et al. (1996) who surveyed cotton field for spiders and found that the population of Aranidae was highest during growing season but were lower during the mid season. Dippenaar et al. (1999) reported that spiders play an important role in keeping pests at endemic levels and preventing outbreaks.

Gustavo and Joao (2003) reported that spiders prey on arthropods belonging to several guilds but spiders showed a preference for wingless prey. The predator P. birmanica consumed moderate number of specimens of all applied prey species than others. Sebastian et al. (2003) found that P. birmanica is one of the dominant spider observed in cotton ecosystem the predation was found varying in different developmental stages and between the sexes adult female were found to the having a higher feeding capacity on four insect pests (Aphid, Amrasca biguttula, Thricertrus bicolor, Heliothis armegera and Helicoverpa armigera. Laboratory conditions are always the controlled conditions and we can achieve the desired results by adopting the appropriate methodologies. More accurate and precise results, which would be more practical, can be achieved by applying them in the natural field conditions.

Current finding revealed that such methodologies might be proved helpful in determining the predatory efficiency of spider against insect pest, specially jassid, in the fields.

REFERENCE

Anonymous, (2006). Area and production of cotton crop. National fertilizer development centre, Islamabad. Pp. 50.

Alvi, Z. H. (2007). Taxonomical and ecological studies of spiders from some fruit gardens of district Jhang, Pakistan, M. Phil Thesis (Un-published), Deptt. of Wildlife and Fisheries, Govt. College Univ., Faisalabad, Pakistan.

Channa, S., M. Kalwar, M. M. Kandhro and R. Kumbhar (2006). Magnitude of gene action for different economic traits of upland cotton, Gossypium hirsutum L. Pakistan J. Agri., Agril. Engg., Vet. Sc. 22(2): 16-20.

Dippenaar, A. S., A. M. V. Berg and A. V. Berg (1999). Spiders in South African cotton fields: species diversity and abundance (Arachnida: Araneae). African Plant Protection. 5(2): 93-103.

Dyal, S. (1935). Fauna of Lahore: Spiders of Lahore. Bull. Zool. Punj. Univ., 1: 119-252.

Dumka, D., C. W. Bednarz and B. W. Maw (2004). Delayed initiation of fruiting as a mechanism of improved drought avoidance in cotton. Crop Sci. 44: 528-534.

Duncan, D. B. (1955) Multiple range and multiple F- tests. Biometrics, 11: 1-42.

El-Heneidy, A. H., A. A. Ibrahim, Y. H. Fayad and G. M. Moawad (1996). Survey and population

dynamics of common true spiders in Egyptian cotton fields. Annals of Agricultural Science, Moshtohor. 34(3): 1177-1187.

Ghafoor, A. (2002). Taxonomic and some ecological studies of the cursorial spiders of cotton fields at Faisalabad (Pakistan). Ph.D Thesis. 275p. Ghafoor, A. and M. Ansar (2011). Population dynamics of the araneid fauna from district Gujranwala, Pakistan.The J. Anim. Plant Sci., 21(4): 812-816

Ghavami, S., M. Taghizadeh, G. Amin and Z. Karimian (2007). Spider (Order: Aranidae) Fauna of cotton Fields in Iran. J. Applied Biolo. Sci. 1 (2): 07-11.

Gustavo, Q. R. and V. N. Joao (2003). Natural history of Misumenops argentteus (Thomisidae): Seasonality and diet on Trichogoniopsios adenantha (Asteraceae). The Journal of Arachnology, 31: 297-304.

Hodge, M. A. (1999). The implications of Intraguild Predation for the Role of Spiders in Biological Control. Journal of Arachnology, 27(1): 351-362.

Khoso, A. W. (1994). Growing Vegetables in Sindh and Balochistan. 2nd Ed. Allied Printing Corporation, Hyderabad. 136pp.

Maqsood, I. (2011). Taxonomical and ecological studies of spiders from some citrus and guava fruit gardens of district Faisalabad, Pakistan M. Phil thesis (un-published), Deptt. Of zoology, govt. college Univ., Faisalabad, Pakistan.

Rajeswaran, J., P. Duraimurugan and P. S. Shanmugam (2005). Role of spiders in agriculture and horticulture ecosystem. Food, Agriculture and Environment (JFAE), 3(3 and 4):147-152.

Sebastian, P. A. and A. V. Sudhikumar (2003). Feeding potential of spiders (Order: Araneae) on Aphis craccivora Koch occurring on cotton. Entomon. 28(2): 153-156.

Steel, R. G. D., J. H. Torrie and D, A, Dickey (1997). Principles and Procedures of Statistics: A Biometrical Approach 3rd Edi.. McGraw Hill Book Co., Inc., New York.

Tikader, B. K. (1982). The Fauna of India: Araneae: Araneidae. Zool. Surv. India, 2: 1-293.

Department of Entomology, **Department of Plant Protection, Sindh Agriculture University, Tandojam, *Department of Wildlife and Fisheries, G. C. University, Faisalabad, Corresponding author: draggondal@yahoo.com
COPYRIGHT 2012 Asianet-Pakistan
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2012 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Publication:Journal of Animal and Plant Sciences
Article Type:Report
Geographic Code:9INDI
Date:Sep 30, 2012
Words:3003
Previous Article:BIOACCUMULATION OF CHROMIUM (III) IN SILKWORM (BOMBYX MORI L.) IN RELATION TO MULBERRY, SOIL AND WASTEWATER METAL CONCENTRATIONS.
Next Article:FEEDING POTENTIAL OF CHRYSOPERLA CARNEA AND CRYPTOLAEMUS MONTROUZIERI ON COTTON MEALYBUG, PHENACOCCUS SOLENOPSIS.
Topics:

Terms of use | Privacy policy | Copyright © 2020 Farlex, Inc. | Feedback | For webmasters