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ACUTE TOXICITY II: EFFECT OF ORGANOPHOSPHATES AND CARBMATES TO CATLA CATLA FINGERLINGS.

Byline: Ghazala S. Mahboob K. A. Al-Ghanim S. Sultana H. F. Alkahem Al- Balawi T. Sultana F. Al-Misned and Z. Ahmed

: Abstract

Pesticides are widely used in modern agriculture to aid in the production of high quality food. However some pesticides have the potential to cause serious health and/or environmental damage. Organophosphates (OCP'S) and carbamates can cause pollution in freshwater ecosystem as well as having a significant effect on the health of fish. The acute effects of commercial formulation of triazophos profenofos carbofuran and carbaryl were determined in one of the indigenous fish species Catla catla fingerlings. Pesticides were applied to fingerlings that had been grown under optimised standard conditions under a maintained static bioassay system. Probit analysis was used for the estimation of LC50 values which were ascertained as 4.84 0.19 0.99 and 7.89 mg/L for triazophos profenofos carbofuran and carbaryl respectively.

100% mortality of Catla catla was observed with a 2.8 mg/L dose of carbofuran at 96 hours with a significant difference. Acute toxic stress was noticed with subjects exhibiting behavioral intoxication including suffocation lying on the bottom erratic swimming lethargy and downward movements and gulping prior to mortality.

Key words: Catla catla Acute toxicity Profenofos Triazophos Carbofuran Carbaryl.

INTRODUCTION

The living world is heavily dependent on chemicals (natural and synthetic) growing demand of food for increasing population has led to substantial production and application of various agro-chemicals (pesticides and fertilizers). Increased use of chemicals particularly affecting man and environment and increased the burden of these chemicals in the environment due to non biodegradability of some of these compounds (Tripathy et al. 2002; Kumar al. 2005). The living world is heavily dependent on chemicals (natural and synthetic) growing demand of food for increasing population has led to substantial production and application of various agrochemicals (pesticides and fertilizers). Among anthropogenic contaminants pesticides are widely detected in freshwater and marine ecosystems. These chemicals are spread on terrestrial cultures and enter waterways from agricultural and urban runoff (Var'o et al. 2008).

It can produce adverse effects on non target aquatic organisms living in areas near agricultural fields. It often ends up in aquatic habitats carried up by the wind runoff or through uncontrolled waste disposal. Fish and aquatic animals as non- target species are exposed to pesticides in three primary ways (i) dermally (direct absorption through the skin) (ii) breathing in contaminated water (uptake through the gills) (iii) orally (drinking or feeding on pesticide-contaminated water or pesticide contaminated prey) (Mathur and Singh 2006).

The organophosphates (OP) and carbamates (Cs) are modern synthetic insecticides and are potent neurotoxic molecules (Lundebye et al. 1997) which are commonly used in the Mediterranean area to treat a variety of agricultural pests (Vioque-Fern'andez et al. 2007; Banni et al. 2005; Ghazala et al. 2014).The risk that a pesticide poses to the surrounding environment depends on its toxicity to fish and other organisms and their exposure to the pesticide. With high effect wide in variety rapid degradation and low toxic residues such pesticides are widely used as pesticides in Pakistan. Poisoning/toxicity is categorised as either acute or chronic and the determination of the median lethal concentration (LC50) is considered to be the preliminary step for studies into the extent of acute or chronic toxicity The short term toxicity of a chemical either natural or man-made is measured using the LC50 (lethal concentration) value.

An LC50 is a measure of how much product is required to kill 50% of the test population over a period of time.

Poisoning/toxicity is categorised as either acute or chronic and the determination of the median lethal concentration (LC50) is considered to be the preliminary step for studies into the extent of acute or chronic toxicity. Different pesticides however have different LC50 values in different organisms (Mathur et al. 2006). Thus Pentachlorophenol (PCP) has an LC50 value of 0.58 ppm in H. fossilis (Farah et al. 2004) while in Lepomis macrochirus the LC50 values of diazinon chlorfevinifos and profenofos were calculated as 2.5 ppm 2.9 ppm and 300 ppb respectively (Dembele et al. 2000; Tomlin 1994). Likewise acute toxicity of profenofos and triazophos in crucian carp was determined as occurring at 0.192 ppm and 8.4 ppm respectively (Jin et al. 2010) whereas in common carp acute toxicity of profenofos (LC50) and triazophos (LC100) was determined as occurring at 62.4 ppb and 1.00 ppm respectively in two different studies (Ismail et al. 2009).

Assis et al. (2010) determined the LC50 values for carbaryl and carbofuran as 33.8 mol/L and 0.92 mol/L respectively while Boran et al. (2007) investigated acute toxicity of carbaryl in Oncorhynchus mykiss and Poecilia reticulate and Beauvais et al. (2000) meanwhile showed that acute malathion intoxication led to swimming and locomotory dysfunction in rainbow trout larvae. Catla catla is one of the indigenous and fast growing freshwater fish. This fish is facing serious threats due to indiscrimninate use of pesticides in the country. Catla catla is surface feeder and is more vulnerable to the all kind of pollutants in freshwater ecosystem in the country.This fish has been selected for this because of its commercial importance. The present study was undertaken to estimate the acute toxicity of commonly used organophosphates and carbamates in Pakistan for fingerlings of Catla catla which is one of the indigenous fish in the Indo-Pak regions.

MATERIALS AND METHODS

Live fingerlings (L=2.65-3 inch W= 18-21 g) of Catla catla were maintained in 70 liter glass aquaria at the Department of Zoology GC University Faisalabad Pakistan having been transferred from the Fish Seed Hatchery Satiana road Faisalabad Pakistan. During acclimatization (15 days) the fish were fed with commercial feed at 3% body weight. Water parameters (electrical conductivity pH and temperature) were analyzed and maintained at optimal conditions. The temperature of the water was regulated at 27 1oC.Aquaria were continuously aerated except at the time of feeding so as the level of dissolved oxygen did not drop below 4.0 mg/L. The electric conductivity and the pH of water were 2.702.80 mS and 8.859.40 respectively.

Test chemicals: Technical grades of triazophos 90% [diethyl o-(1-phenyl-1h-124-triazol-3-yl) phosphorothioate)] profenofos 98% [O-(4-Bromo-2- chlorophenyl) O-ethyl S-propyl phosphorothioate] carbaryl 97%(1-naphthyl methylcarbamate)] and Carbofuran 90% (23-dihydro-22-dimethyl-7- benzofuranyl methylcarbamate) were obtained from Ali Akbar Enterprises Lahore Pakistan. Triazophos was dissolved in Methanol (Analytical grade Merck) profenofos was dissolved in Acetone (Analytical grade Merck) carbofuran and carbaryl were dissolved in ethanol (Analytical grade Merck). To confirm the solubility of pesticides in water 1ppm concentration of each pesticide dissolved in relevant solvent in the test water sample was prepared and was confirmed with HPLC (Model L7400). The Solid phase extraction technique was used for the extraction of pesticide residues from water sample (TOTOLIN 2003).

Since toxicity test fish were divided into six groups each with 10 individuals following random selection to test 6 dilution (for each pesticide) in triplicates with negative control receiving no pesticide but maximum solvent that any dosing solution contain. Before acute toxicity tests (LC50) preliminary tests were done with 1ppm and 10ppm concentration of all pesticides to determine the minimum and maximum limits of viability and mortality of fish fingerlings.

Determination of sub lethal concentration and acute toxicity test: Four day static toxicity tests were performed to determine the LC50 values (OECD 1992). Stock solutions of the pesticides were made by dissolving profenofos in acetone triazophos in methanol and both carbofuran and carbaryl in ethanol. Six dosing solutions were prepared from the stock prepared by mixing different proportions of stock solution in acetone (profenofos) triazophos (methanol) and ethanol (carbofuran and carbaryl) to get the desired concentrations. The nominal concentrations of active tested ingredients for all fish fingerlings were determined by following OECD (1992). Nominal concentrations of active ingredient tested were: triazophos 4 5 6 7 8 8.5 9; profenofos 0.098 0.196 0.284 0.392 0.49 0.588 0.686; carbofuran 0.25 0.50 1 2 4 8 and carbaryl 6 7 8 9 10 11 12 mgL-1.

The specimens were fed with commercial feed @ 3 % of their body weight once in a day and the feeding was stopped 24 h prior to the pesticide exposure to till the end of the experiment and water exchange was stopped. Mortality and behavioral changes of the specimens were recorded after 24 48 72 and 96 hours. Median lethal concentrations based on 96 hours acute toxicity were determined according to the guidelines of OECD (1992). LC50 of triazophos profenfos carbofuran and carbaryl and the 95% condence limits were calculated by a computer program [TSK (Trimmed SpearmanKarber) program (1991) Version 1.5] as described by Finney's Probit Analysis LC50 Determination Method (1978).Data for mortality and a live fish was analyzed using Probit analysis (Finney 1971). The behavioral changes in the specimens were also noted right after the application of testing dose till the end of an experiment.

The behavioural changes of the healthy fish and the fish subjected to various doses of tested pesticides were evaluated as regard to behaviour anomalies. A guide covering some general information on methods for qualitative and quantitative assessment of the behavioral responses of fish (locomotory activity feeding and social responses) during standard laboratory toxicity tests to measure the sublethal effects of exposure to chemical substances were determined as described by ASTM ( 2008). The negative control group was also monitored in the same way for mortalities and change in behaviour including loss of balance moving in a spiral fashion with jerks lying laterally and opened mouth with rapid opercular movements. In addition LC50 values were compared by the method of APHA (1995).

Quality- assurance measures applied in the laboratory included rigorous contamination-control procedures (strict washing and cleaning procedures) monitoring of blank levels of solvents equipment and other materials analysis of procedural blanks recovery of spiked standards monitoring of detector response and linearity and analysis of a reference material. Recoveries of pesticides in the reference material were between 80 and 110 % of certified concentrations.

RESULTS AND DISCUSSION

Effect of pesticides on behavior of the fish: In the present study the control fish were active for feeding and alert to slightest of the disturbance with their well- synchronized movements. The behavior did not significantly vary between the control groups; therefore these results were taken as standards for the entire experimentation. The effects of pesticide intoxication were observed as suffocation restlessness loss of equilibrium and erratic swimming on prodding with all the tested pesticides. This followed loss of co-ordination and occupancy of twice the area to that of control group were the early responses of the fish following exposure to sodium cyanide in both the sublethal concentrations. Subsequently fish moved to the corners of the test chambers which can be viewed as an avoidance behavior of the fish to profenofos triazophos carbofuran and carbaryl.

Further fish exhibited irregular erratic and darting swimming movements and loss of equilibrium followed by hanging vertically in water. Fish often remained at the bottom with mouth opened before dying (Table 1). Behavioral responses meet the criteria as rapid tool for bioassay testing and could be easily standardized using pesticides as reference toxicant. The development of behavioral methods in fish is an important tool in aquatic toxicology. Behavioral responses represent an integrated response of fish species to toxicant stress (Kane et al. 2005). Changes in spontaneous locomotor activity and respiratory responses are sensitive behavioral indicators of sublethal exposure in fish (Scherer 1992). Behavior provides a unique perspective linking the physiology and ecology of an organism and its environment (Little and Brewer 2001). Avoidance response and locomotor activity are referred to the same category of behavioral responses as described by Scherer (1992).

However avoidance response can be initiated through chemosensory irritation since it was established that the fish olfactory system is involved in the formation of an avoidance response to heavy metals (Sveceviceius 1991). Locomotor activity may reflect a more non- specific stress response resulting in changes in blood cortisol and glucose levels.

Determination of acute toxicity: Mortality response and relationship of selected fish to various concentrations of pesticides are presented in Table (2) and Figures (1-4). An increase in the number of mortalities was observed as the concentration of insecticide was increased. There was no mortality in the control group as well as in the group receiving 4 mg/L and 2 mg/L (lowest dose) of triazophos. Catla catla fingerlings died after 2-3 hours of exposure at 8 mg/L. In the case of profenofos a dose dependent increase and time dependent decrease were observed in the mortality rate at the exposure time increased from 24 to 96 hours; i.e. the median concentration was reduced. There was a significant difference (P less than 0.05) among LC50 values obtained at different times of exposure.

At 96 hours median lethal concentrations were recorded as 0.19 mg/L (0.14- 0.24). The 100% mortality of Catla catla was observed with a 2.8 mg/l dose of carbofuran at 96 hours with a significant difference. LC50 values of carbofuran at 24 and 96 hours were estimated as 2.40 mg/L (1.76-3.30) and 0.99 mg/L (0.73-1.35) respectively. Median lethal concentrations of carbaryl at 24 hours 48 hours 72 hours and 96 hours were observed in Catla catla as being as 9.49 mg/L (8.91-10.08) 9.10 mg/L (8.50-9.76) 8.42 mg/L (7.85-9.09) and 7.89 mg/L (7.31-8.67) mg/l (7.65-8.90) respectively at 95% confidence intervals (Table 2). An overall comparison of all the tested pesticides from toxicity point of revealed profenofos as highly toxic at its very low concentrations and caused mortality in Catla catla. The least toxic compound was found to be carbaryl as compared to other tested compounds.

Median lethal concentration at 24 hours 48 hours 72 hours and 96 hours were observed in Catla catla as 9.49 mg/L (8.91-10.08) 9.10 mg/L (8.50- 9.76) 8.42 mg/L (7.85-9.09) and 7.89 mg/L (7.31-8.67). In general acute susceptibility of the tested pesticides was as follows: profenofos greater than carbofuran greater than triazophos greater than carbaryl.

At 96 hours median lethal concentrations of profenophos and triazophos were 0.19 mg/L (0.14- 0.24) and 4.84 mg/L (4.31- 5.42) in Catla catla respectively (Table 2). The results of the present study showed a higher median lethal concentration but nearly similar effects on the behaviour of fish compared to the findings of Pandey et al. (2011) where acute toxicity of profenofos to Channa punctuates was observed as 2.68 g/L. The effects of intoxication with profenofos presented as erratic swimming hyperexcitability discoloration of the skin and secretion of mucus in the body and the gills leading eventually to death.

In the current study 96 hours median lethal concentrations of carbofuran was estimated at 0.99 mg/L (0.73- 1.35) while median lethal concentrations of carbaryl at 24 hours 48 hours 72 hours and 96 hours were observed in Catla catla as 9.49 mg/L (8.91 - 10.08) 9.10 mg/L (8.50 - 9.76) 8.42 mg/L (7.85- 9.09) and 7.89 mg/L (7.31 - 8.67) at 95% confidence intervals respectively (Table 2). Assis et al. (2010) determined the LC50 values for carbofuran and carbaryl as 0.92 mol/L and 33.8 mol/L respectively in Arapaima gigas. In this present study there was also a higher median lethal concentration of carbaryl compared to carbofuran. Acute toxicity of carbaryl was also investigated by Boran et al. (2010) in Oncorhynchus mykiss and Poecilia reticulate. Hernandez-Moreno et al. (2011) investigated the acute effects of carbofuran on sea bass (Dicentrarchus labrax).

The observed values of LC50 of carbofuran and carbaryl in current studies are in agreement with those calculated with cabofuran and carbaryl for common prawn (Assis et al. 2010).

When fish were exposed to pesticides they displayed the signs of intoxication in behavioral alterations such as loss of equilibrium and agitation. These symptoms were followed by increased respiratory rhythm and increased opercular movement and ended with the intermediate period where fish lay at the bottom of the aquaria exhibiting muscular weakness and erratic movement (Table 1). Although different fish species may manifest different behavioral responses these observations observed in Catla catla are similar to those observed by Da Silva et al. (1993) on Callichtys callichtys exposed to Folidol 600 (pesticide) and those observed by Fernandez-Vega et al. (1999) and Farah et al. (2004). The behavioral effects on fish of intoxication with organophosphates and carbamates that were observed in this study could be linked to a failure in energy production or release of stored metabolic energy of severe stress ultimately leading to fish death as reported by Chakraborty et al. (1989).

Table 1: Effect of triazophos profenofos carbofuran and carbaryl on the behavior of Catla catla

Visual effects###Suffocation###Laying on###Erratic###Opening of###Lethargic###Downward###Gulping

###the bottom###swimming###mouth and###movements###movement###before

###gills###death

Triazophos###+++++###+++++###++++###+++###_###_###+++

Profenofos###+###++###+++###++++###+++++###+++++###+++++

Carbofuran###++###_###++###_###+++###_###_

Carbaryl###++###_###_###_###++++###_###++

Table 2: Median lethal concentration (LC50) of triazophos profenofos carbofuran and carbaryl in Catla catla at

###different time intervals.

Pesticides###Points###24Hours###48Hours###72hours###96Hours

Triazophos (mg/L)###LC50###6.64###5.83###5.64###4.84

###Lower and upper confidence limits###6.15- 7.13###5.35- 6.32###4.98- 7.85###4.31- 5.42

###(95%)

Profenofos (mg/L)###LC50###0.33###0.29###0.25###0.19

###Lower and upper confidence limits###0.26- 0.39###0.23- 0.37###0.20-0.30###0.14- 0.24

###(95%)

Carbofuran (mg/L)###LC50###2.40###1.81###1.31###0.99

###Lower and upper confidence limits###1.76- 3.30###1.26- 3.31###0.94- 1.92###0.73- 1.35

###(95%)

Carbaryl (mg/L)###LC50###9.49###9.10###8.42###7.89

###Lower and upper confidence limits###8.91 - 10.08###8.50 - 9.76###7.85- 9.09###7.31 - 8.67

###(95%)

Kamanyire and Karalliedde (2004) reported that in addition to acute symptoms some organophosphates can cause other symptoms usually appear 1 4 days after exposure or poisoning with organophosphates such as weakness in the muscle and breathing difficulties and the observations in the present study are in line with these findings. Symptoms of acute pesticide poisoning can be divided according to the site of acetylcholine accumulation in the organism. Acetylcholinesterase (Ach) remains active throughout the nervous system in the fish but contaminants may interfere with the cholinergic neural transmission even in the case of sublethal exposure intoxication may be in the form of interference with carbohydrate metabolism reproduction and behaviour (Banerjee et al. 1999). Neurotoxicants may also impair cholinergic neural transmission primarily through cholinesterase (ChE) inhibition in acute toxicity (Pavlov et al. 1992; Marrs 1993).

This current study shows that profenofos was highly toxic as compared to the other pesticides studied in the context of LC50 values in that only low concentrations caused death of Catla Catla. If we compare the toxicity of other pesticides then it would be apparent that carbaryl was less toxic in Catla catla and also caused the least effects on behaviour. Owing to the highly toxic effects of these pesticides they must be properly monitored in the environment so that their toxic effects on non-target organisms can be reduced.

Acknowledgements: The authors would like to their sincere appreciation to the Deanship of Scientific at King Saud University for its funding of this research through the Research Group Project No. RGP-1435-012.

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