A study of changes in liver function biomarkers among malaria infected patients.
MATERIALS AND METHODS: Study centre and period: This duration based study was done from January 2012 to March 2013 in Department of Biochemistry, VIMS & RC, and Bangalore.
SUBJECT SELECTION: Patients selection was done by simple random sampling from those presenting at VIMS & RC, Bangalore, with a history of fever, chills and malaise & who were subsequently confirmed to be malaria positive by Geimsa stained peripheral blood film. Based on the following selection criteria 36 patients were selected. 36 subjects in apparent good health and malaria parasite negative were included as control individuals. Consent was sought and obtained from both cases and controls.
EXCLUSION CRITERIA: Patients on self-medication with any anti-malarial drugs prior to presentation and patients with any type of liver disease were excluded from the study.
SPECIMEN COLLECTION AND ASSAY: 5ml of venous blood sample was taken by aseptic precaution. Samples were centrifuged and serum AST, ALT, ALP, TP, ALB, TB & DB were measured by using auto analyzer Beckman Coulter Synchron DxC by using Beckman Coulter Kits (USA).
STATISTICAL ANALYSIS: The data obtained were analyzed using the student's t test and level of significance was set at p<0.05 and were expressed as Mean [+ or -] Standard Deviation (SD)
RESULTS: Table 1 shows the changes in liver function biomarkers of the malarial (case) and control subjects. When the malaria positive (test) patients were compared with the non-infected subjects, there was increase in the mean activity values of the various liver enzymes, serum total and direct bilirubin. However, serum total protein and albumin levels were reduced among the malaria infected patients.
Changes in liver enzymes (AST, ALT and ALP) activity and total & direct bilirubin values for the malaria patients were significantly (p<0.05) higher than those for the non-malaria infected subjects.
DISCUSSION: Malaria is endemic in India with an estimated 70-100 million cases each year. Of these 45-50 % are plasmodium falciparum. (6)
Malaria is a mosquito-borne tropical disease caused by the Plasmodium species of protozoa. It affects mainly the hepatocytes and red blood cells (RBCs) and manifests clinically as fever and splenomegaly. (7)
LIVER DISFUNCTION: Mild hemolytic jaundice is common in malaria, severe jaundice is associated with P. falciparum infections, is more common among adults than among children and results from hemolysis, hepatocytic injury and cholestasis. When accompanied by other vital organ dysfunction (often renal impairment) liver dysfunction carries a poor prognosis. Hepatic dysfunction contributes to hypoglycemia, lactic acidosis and impaired drug metabolism. The hemolysis results from the invasion of the erythrocytes by malarial parasites. P. malariae is known to invade mature RBCs, whereas P. vivax and P. ovale are known to invade young RBCs. P. falciparum parasites are capable of invading RBCs of any age and hence can lead to very high levels of parasitemia; while parasitemia is limited in all other types of Plasmodium infections. (8)
In this study, it was observed that the values for liver function profiles among patients with malaria were elevated when compared with those without infection. The observed increase (p<0.005) in serum liver enzymes (AST, ALT and ALP) could be due to leakage from hepatic cells that were killed or injured by the auto-immune progress and / or by abnormal cell activation induced by the parasites. This finding supports previous reports. (2)
Total protein and albumin levels were reduced in malaria patients may be due to the fact that liver, which is the primary organ for protein synthesis, is the site for parasite multiplication in case of malaria. It can also be a part of the acute phase response. The reduced TP & ALB finding supports previous reports. (3)
According to WHO, jaundice is one of the important manifestations of severe malaria. There is evidence of focal hepatocyte necrosis, cholestasis, bile stasis, granulomatous lesion or malarial nodules. (4) Anand et al conducted a study on 39 patients with falciparum malaria and jaundice, out of whom 13 had serum bilirubin around the mean value of 16.3%, and most had predominantly conjugated hyperbilirubinemia (5) Especially noted is the elevation of alanine transaminase to more than three times the upper limit of normal value. (9) The elevation of serum transaminases in falciparum malaria patients is more than 5 times the upper limit of reference range. (10)
CONCLUSION: Hepatic involvement is a common accompaniment of acute malaria infection, and hepatic dysfunction ranges from a mild elevation of liver enzymes to the range of acute hepatitis. The presence of hepatitis in malaria patients indicates a more severe illness with a higher incidence of complications, multiorgan failure and a bad prognosis.
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(9.) Bhalla A, Suri V, Singh V. Malarial hepatopathy. J Postgrad Med 2006; 52: 315-320.
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Shwetha M. S 
[1.] Shwetha M. S.
PARTICULARS OF CONTRIBUTORS:
[1.] Tutor, Department of Biochemistry, MIMS, Mandya.
NAME ADDRESS EMAIL ID OF THE CORRESPONDING AUTHOR:
Dr. Shwetha M. S, W/o Dr. Jagadeesh C. P, # 6, B 33, Shakthi Nagar, Mysore. Email: email@example.com
Date of Submission: 31/10/2014.
Date of Peer Review: 03/11/2014.
Date of Acceptance: 06/11/2014.
Date of Publishing: 07/11/2014.
Table 1: Liver function biomarkers of cases and controls Variables Malaria cases Controls (N=36) (N=36) Mean [+ or -] Mean [+ or -] SD SD Total Bilirubin(mg/dl) 1.96 [+ or -] 3.47 0.53 [+ or -] 0.20 Direct Bilirubin(mg/dl) 0.84 + 1.85 0.09 + 0.05 Total Protein(g/dl) 7.64 + 9.34 7.10 + 1.30 Albumin(g/dl) 2.80 + 0.34 4.37 + 0.46 AST(IU/L) 38.75 + 20.32 24.72 + 5.31 ALT(IU/L) 26.92 + 8.73 19.88 + 6.76 ALP(IU/L) 77.19 + 28.18 94.59 + 37.3 Variables P-value Total Bilirubin(mg/dl) 0.023 * Direct Bilirubin(mg/dl) 0.026 * Total Protein(g/dl) 0.749 Albumin(g/dl) 6.551 AST(IU/L) 0.000 * ALT(IU/L) 0.000 * ALP(IU/L) 0.032 * * Significant
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|Title Annotation:||ORIGINAL ARTICLE|
|Publication:||Journal of Evolution of Medical and Dental Sciences|
|Date:||Nov 10, 2014|
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