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A specific HPLC-UV and fluorescence method for the detection of three anti-depressant drugs in various water systems.

Pharmaceuticals in water are considered as a major emerging pollutant because of their ubiquity in the aquatic environment and their health effects. A new, fast and economical HPLC method was developed for the analysis of carbamazepine, venlafaxine and fluoxetine in water systems.

A gradient reverse-phase HPLC assay was used with UV and fluorescence detectors. Sample was passed through Gemini C18 110A (250 x 4.60 mm, 5 pm, Phenomenex) column at a flow rate of 1.0 ml/min. A mixture of citric acid and EDTA was mixed in water and was used as a solvent A. Mobile phase was made by mixing solvent A and methanol. 4L of water samples were collected and separated by solid-phase extraction procedure, using the (Oasis HLB, 30 pm) cartridges on a Vac Elut apparatus and an HPLC run was performed.

From spiking experiments, limit of detection (LODs) and limit of quantification (LOQs) for carbamazepine were 10 ng/l and 100 ng/l, for venlafaxine were 1 [micro]g/l and 1 ng/l, and for fluoxetine were 100 ng/l and 1 [micro]g/l, respectively.

HPLC can be used to detect the trace amount of pharmaceuticals in water. The technique requires no derivatization steps, requires less time and is more cost-effective.

Gaurav Sharma (G), Idaho State University

Dr. James C. Bigelow, Idaho State University

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Author:Sharma, Gaurav "G"; Bigelow, James C.
Publication:Journal of the Idaho Academy of Science
Article Type:Brief article
Geographic Code:1U8ID
Date:Dec 1, 2014
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