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A newly discovered variant of a hantavirus in apodemus peninsulae, far eastern Russia. (Letters).

To the Editor: Hemorrhagic fever with renal syndrome (HFRS) is caused by Hantaan virus (HTNV) or Seoul virus (SEO) in Asia and Puumala virus (PUUV) or Dobrava virus (DOBV) in Europe (1). Each of these hantaviruses is predominantly associated with a single rodent species as its primary natural reservoir: HTNV with the striped field mouse Apodemus agrarius, SEO with Rattus norvegicus, PUUV with the bank vole Clethrionomys glareolus, and DOBV with the yellow-necked mouse Apodemus flavicollis. An additional rodent reservoir of DOBV, A. agrarius, was reported recently (2).

The first HFRS cases (then called "hemorrhagic nephroso-nephritis") were clinically described in the Amur River basin during the 1920s by Russian scientists (3). Serologic studies suggest that numerous hantaviruses are present in humans and rodents in the far east of Asian Russia (4-5). Serologic evidence of hantavirus infection in A. agrarius, A. peninsulae (Korean field mouse), R. norvegicus, Cl. rufocanus, Cl. rutilus, and Microtus fortis has been reported (5). Only Khabarovsk virus (KBR), isolated from M. fortis, has been characterized in detail, and no association with human disease was established (6).

To genetically characterize hantaviruses in A. peninsulae, we studied samples from rodents captured in July and August 1998 in the same region of the forest near Khabarovsk. Lung-tissue samples were screened by enzyme-linked immunosorbent assay for HTNV/SEO/PUUV-related antigen. Samples from four hantavirus-positive rodents were tested by reverse transcription and nested polymerase chain reaction (PCR). Four M-segment PCR products (nt 2639-3000) and two S-segment PCR products (nt 592-945) were produced and directly sequenced (GenBank accession numbers AF332569-AF332573). All sequences were closely related to each other, with nucleotide diversity between strains not exceeding 0.6% for M segments and 1.3% for S segments. Comparative analysis of the M segments showed that hantaviral nucleotide sequences from A. peninsulae were very similar to those we identified earlier in HFRS patients (diverging 3.1% to 6.6%), which we term the Amur genotype of HTNV (7). The S-segment sequences of the AMR genotype from human patients were not available for comparison. The nucleotide sequence (the M and S segments, respectively) of the hantavirus detected in A. peninsulae diverged substantially from those of other hantaviruses (15% and 19% for HTNV, 21% to 28% for SEO, 22% and 29% for DOBV, 38% and 39% for PUUV, and 36% and 37% for KBR).

Neighbor-joining phylogenetic analysis based on partial sequences of the S segment indicated that the hantaviral sequences from A. peninsulae form a separate lineage on the phylogenetic tree, and together with HTNV virus strain 76-118, which originates from A. agrarius, constitute a well-supported group. A phylogenetic tree based on partial M segment sequences placed all hantavirus strains originating from A. peninsulae or from HFRS patients apart from all HTNV sequences recovered from A. agrarius (strain 76-118) and HFRS patients from Korea (strains HoJo, Lee). The taxonomic placement of this hantavirus (Amur genotype) as a distinct hantavirus or a distinct genetic lineage of HTNV remains to be determined. In addition, the finding of distinct DOBV genetic lineages in A. flavicollis and A. agrarius raises the same question of whether the two DOBV variants represent distinct hantaviruses (2).

A. peninsulae is widely distributed throughout eastern Asia, from Altai and south Siberia to the Russian far east, northeastern and eastern parts of China, and Korea. A survey of hantavirus antigens in rodent populations in the far east of Russia demonstrated the presence of HTNV-like antigen in 8% to 16% of A. peninsulae (5). Whether pathogenic AMR genotype of virus exists in A. peninsulae throughout far eastern Asia, from Russia to China and Korea, requires further study. Comparing hantaviral genome sequences available from GenBank shows that the M segment nucleotide sequence recovered from an HFRS patient from China (strain H8205, GenBank accession number AB030232) was very similar to the AMR genotype from A. peninsulae (94% to 96% identity), suggesting that this hantavirus is also present in A. peninsulae in China.

In earlier studies, we found that sera from patients infected by the AMR genotype of hantavirus showed extensive cross-reactivity with HTNV and SEO antigens in immunofluorescent antibody tests (7). Consequently, many HFRS cases previously thought to have been caused by HTNV or SEO may instead have been caused by infection with the hantavirus described here.

Our data represent the first genetic evidence for the AMR genotype of HTNV in A. peninsulae and suggest that this rodent species may be a natural reservoir for this pathogenic hantavirus.

This work was supported by the Ministry of Sciences of the Russian Federation and the National Academy of Sciences of the United States through the International Science and Technology Center, grant # 805.

Lyudmila Yashina, * Vasiliy Mishin, * Nina Zdanovskaya, ([dagger]) Connie Schmaljohn, ([double dagger]) and Leonid Ivanov ([dagger])

* State Research Center of Virology and Biotechnology "Vector," Koltsovo, Novosibirsk, Russia; ([dagger]) Khabarovsk Antiplaque Station, Khabarovsk, Russia; and ([double dagger]) U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA


(1.) Schmaljohn C, Hjelle B. Hantaviruses: a global disease problem. Emerg Infect Dis 1997;3:95-104.

(2.) Avsic-Zupanc T, Nemirov K, Petrovec M, Trilar T, Poljak M, Vaheri A, et al. Genetic analysis of wild-type Dobrava hantavirus in Slovenia: co-existence of two distinct genetic lineages within the same natural focus. J Gen Virol 2000;81:1747-55.

(3.) Casals J, Henderson BE, Hoogstraal H, Johnson KM, Shelokov A. A review of Soviet viral hemorrhagic fever, 1969. J Infect Dis 1970;122:435-53.

(4.) Astakhova T, Slonova R, Tkachenko E, Bondarenko A, Kosoy M, Kushnarev E. Study of the role of hantavirus serotypes in the etiology of hemorrhagic fever with renal syndrome in the Far East of the USSR. Vopr Virusol 1990;35:492-4. [In Russian.]

(5.) Kosoy M, Slonova R, Mills J, Mandel E, Childs J. Community structure and prevalence of hantavirus infection in rodents: a geographic division of the enzootic area in Far Eastern Russia. J Vect Ecol 1997;22:52-63.

(6.) Horling J, Chizhikov V, Lundkvist A, Jonsson M, Ivanov L, Dekonenko A, et al. Khabarovsk virus: a phylogenetically and serologically distinct hantavirus isolated from Microtus fortis trapped in far-east Russia. J Gen Virol 1996;77:687-94.

(7.) Yashina L, Patrushev N, Ivanov L, Slonova R, Mishin V, Kompanez G, et al. Genetic diversity of hantaviruses associated with hemorrhagic fever with renal syndrome in the far east of Russia. Virus Res 2000;70:31-44.
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Author:Ivanov, Leonid
Publication:Emerging Infectious Diseases
Geographic Code:00WOR
Date:Sep 1, 2001
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