West Nile virus, Venezuela.
To the Editor: West Nile virus West Nile virus, microorganism and the infection resulting from it, which typically produces no symptoms or a flulike condition. The virus is a flavivirus and is related to a number of viruses that cause encephalitis. (WNV; genus Flavivirus; family Flaviviridae) has been perpetuating in North America since 1999 (1). However, its status as a self-perpetuating pathogen in South America remains uncertain. Infected horses and birds have been reported in various Caribbean Islands, Mexico, and northern Central America (2,3). In South America, isolated reports of infected dead-end hosts (horses) have come from northern Colombia and Argentina, but they lack evidence for infection in avian amplifying hosts (4,5). We report serologic evidence of establishment of WNV in South America.
Serum samples from birds and horses from 33 locations in Venezuela (Online Appendix Table, available from http://www.cdc.gov/EID/content/13/4/651-appT.htm) were screened for immunoglobulin G (IgG) antibodies against WNV antigen by ELISA ELISA (e-li´sah) Enzyme-Linked Immuno-Sorbent Assay; any enzyme immunoassay using an enzyme-labeled immunoreactant and an immunosorbent.
n. (6) and confirmed by plaque reduction neutralization test (PRNT) as previously described (7). The flavivirus generating the IgG response was identified by using the following criteria: 90% inhibition of virus in serum diluted at least 1:40 and 4-fold greater neutralizing antibody titer compared with closely related flaviviruses. IgG antibody against flavivirus was detected by ELISA in 14 of 576 resident birds, including 5 Turdus leucomelas, 3 Gallus Gallus (Caius Vibius Trebonianus Gallus) (găl`əs), d. 253 or 254, Roman emperor after 251. He fought in the eastern campaign that proved fatal to Decius. gallus (captive), 2 Campylorhamphus trochilirostris, and 1 each of Elaenia flavogaster, Coereba flaveola, Thraupis palmarum, and Anisognathus flavinucha.
WNV was confirmed as the etiologic agent of infection in 5 adult birds (3 T. leucomelas [pale-breasted thrush], 1 C. flaveola [bananaquit The Bananaquit, Coereba flaveola, is a passerine bird, the only member of the genus Coereba and the monotypic family Coerebidae. The latter used to contain other nectar-eating birds from the tropical Americas, but these have since been moved. ], and 1 G. gallus [domestic chicken] with the earliest collection date in February 2006); virus neutralization neutralization, chemical reaction, according to the Arrhenius theory of acids and bases, in which a water solution of acid is mixed with a water solution of base to form a salt and water; this reaction is complete only if the resulting solution has neither acidic nor titers ranged from 80 to 320. One serum sample cross-reacted with other flaviviruses tested, with equivalent titers to WNV, Saint Louis encephalitis virus Saint Louis encephalitis virus
An arbovirus that causes Saint Louis encephalitis and is transmitted by a mosquito. (SLEV SLEV Saint Louis Encephalitis Virus
SLEV Surround Level ), and Ilheus virus (ILHV) and was thus considered infected with an undetermined flavivirus. Seven serum samples were negative (antibody titers <20), and 1 sample was not tested because of insufficient sample volume.
Antibody against flavivirus was detected by ELISA in 141 of 791 horses, and 34 (4.3%) were confirmed positive for WNV infection by PRNT; viral titers [greater than or equal to] 640 occurred in half of these horses. The earliest collection date for a WNV-positive horse was February 2004 and the most recent was May 2006. Specific WNV-reactive equine serum samples were distributed in valley regions (prevalence 1.3%), savannah grasslands (2.4%), the western region of Zulia (0.4%) and the Central Lake Basin (0.3%). A total of 46 (5.8%) equine serum samples were positive for neutralizing antibody to SLEV, and 8 (1.0%) samples were positive for neutralizing antibodies to ILHV. Forty-nine samples neutralized at least 2 of the 3 viruses and were classified as undetermined flaviviruses. Serum samples from 2 horses were negative in neutralization assays; 2 others were not tested because of insufficient sample volume.
Detection of WNV-infected resident birds provides strong evidence of the establishment, rather than importation, of WNV in South America. We hypothesize that ornithophilic mosquitoes (such as some Culex Culex /Cu·lex/ (ku´leks) a genus of mosquitoes found throughout the world, many species of which are vectors of disease-producing organisms.
n. spp.), which are present in the area in consistently high numbers, acquired the virus through hematophagous hematophagous
subsisting on blood, e.g. hematophagous flies. feeding on recently infected, migrating birds. Once introduced to local mosquitoes, virus is amplified among susceptible resident birds fed upon by ornithophilic mosquitoes. This pattern allows perpetuation and subsequent establishment of virus in a continuous transmission cycle, as opposed to infection of dead-end hosts, e.g., horses. This is the first report of WNV infection in South American birds and definitive establishment of the virus in South America.
We observed varying WNV seroprevalence rates in birds and horses across regions in Venezuela (Figure). These differences reflect the focal and stochastic nature of arbovirus arbovirus
Any of a large group of viruses that develop in arthropods (chiefly mosquitoes and ticks). The name derives from “arthropod-borne virus.” The spheroidal virus particle is encased in a fatty membrane and contains RNA; it causes no apparent harm to the transmission, which depends upon many ecologic factors. One possible explanation for the greater seroprevalence seroprevalence Immunology The proportion of a population that is seropositive–ie, has been exposed to a particular pathogen or immunogen; the seropositivity of a population is calculated as the number of individuals who produce a particular antibody divided in the central and eastern llanos (savannahs) and valley regions, compared with the coastal western region of Zulia State (p<0.0001, by Pearson's [chi square] test) would be virus introduction by migrating birds by an eastern migration route.
Existence of several closely related flaviviruses in the American tropics (8-10) may convey cross-protection in animals (e.g., ILHV and SLEV) or humans (dengue viruses, yellow fever virus yellow fever virus
An arbovirus of the genus Flavivirus that causes yellow fever and is transmitted by mosquitoes. ), thereby potentially diminishing disease caused by a newly introduced flavivirus such as WNV. Although ILHV infection has not been detected in Venezuela, this flavivirus is prevalent in Brazil, Peru, French Guyana, Trinidad, and Colombia. Our study demonstrated widespread distribution of ILHV in Venezuela. Other South American flaviviruses, such as Bussuquara, Cacipacore, and Iguape, and as yet undiscovered viruses may also circulate in Venezuela.
We encourage those involved in the public and animal health systems in Venezuela to consider zoonotic Zoonotic
A disease which can be spread from animals to humans.
Mentioned in: Zoonosis flaviviruses in the differential diagnoses of human and equine cases of encephalitis and to consider ecologic surveillance for zoonotic flaviviruses in mosquito and vertebrate host populations. We recommend monitoring blood and organ donations for flavivirus infections. Our study sheds light on flavivirus distribution in Venezuela. However, nothing else is known about the ecology of zoonotic flaviviruses in this country. Such knowledge will be essential for designing effective surveillance and control should these viruses be shown to cause human illnesses.
We dedicate this work to the late Andrew Spielman, our coauthor and beloved mentor. We thank J. Rivero, I. Perez, M. Mendez, I. Matheus, M. Aguiar de Bracho, I. Carreno, J.M. Hernandez, A. Nagy, A. Suarez, N. Moncada, M. Kilpatrick, E. Rodriguez, E. Marquez, E. Marian, B. Hernandez; C. Rivero-Blanco, M. Azar, J. Rodriguez, H. Montanez, F. Alfonzo, and G. Rangel for their contributions to this study; and the Centro de Investigaciones Biomedicas, Universidad de Carabobo and the Cell Culture Core of Wadsworth Center, New York State Health Department, for their support. We also thank ProFauna and the National Institute of Parks in Venezuela for permission to obtain mosquito and bird samples.
This study was supported by grant AI45440 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, and an International Collaborations in Infectious Disease Research opportunity pool grant.
Irene Bosch, * Flor Herrera, ([dagger]) Juan-Carlos Navarro, ([double dagger]) Miguel Lentino, ([section]) Alan Dupuis, ([paragraph]) (#) Joseph Maffei, ([paragraph]) (#) Matthew Jones, ([paragraph]) (#) Ernesto Fernandez, ** Nelson Perez, ([dagger])([dagger]) Jorge Perez-Eman, ([double dagger]) Anthony Erico Guimaraes, ([double dagger]) ([double dagger]) Roberto Barrera, ([subsection]) Nereida Valero, ([paragraph]) ([paragraph]) Johanny Ruiz, ([dagger]) Glenda Velasquez, (##) Juan Martinez, ([double dagger]) Guillermo Comach, ([dagger]) Nicholas Komar, *** Andrew Spielman, ([dagger]) ([dagger]) ([dagger]) (1) and Laura Kramer ([paragraph]) (#)
* University of Massachusetts Medical School UMMS is ranked fourth in primary care education among the nation’s 125 medical schools in the 2006 U.S.News & World Report annual guide, “America’s Best Graduate Schools”. UMMS is also a major center for research. , Worcester, Massachusetts, USA; ([dagger]) Universidad de Carabobo Biomed, Maracay, Venezuela; ([double dagger]) Universidad Central de Venezuela, Caracas, Venezuela; ([section]) Coleccion Omitologica Phelps, Caracas, Venezuela; ([paragraph]) New York State Department of Health, Albany, New York For other uses, see Albany.
Albany is the capital of the State of New York and the county seat of Albany County. Albany lies 136 miles (219 km) north of New York City, and slightly to the south of the juncture of the Mohawk and Hudson Rivers. , USA; #State University of New York (body) State University of New York - (SUNY) The public university system of New York State, USA, with campuses throughout the state. at Albany, Albany, New York, USA; ** Universidad Central de Venezuela, Maracay, Venezuela; ([dagger]) ([dagger]) Instituto Nacional de Investigaciones Agricolas, Maracay, Venezuela; ([double dagger]) ([double dagger]) Instituto Oswaldo Cruz Coordinates: Instituto Oswaldo Cruz (FIOCRUZ) is a scientific institution for research and development in biomedical sciences located in Rio de Janeiro, Brazil. It was founded by Dr. Oswaldo Cruz, a noted physician and epidemiologist. , Rio de Janeiro Rio de Janeiro, city, Brazil
Rio de Janeiro (rē`ō də zhänā`rō, Port. rē` thĭ zhənĕē`r , Brazil; ([subsection]) Centers for Disease Control and Prevention Centers for Disease Control and Prevention (CDC), agency of the U.S. Public Health Service since 1973, with headquarters in Atlanta; it was established in 1946 as the Communicable Disease Center. , San Juan, Puerto Rico San Juan (IPA: [saŋ hwaŋ]) (from the Spanish San Juan Bautista, "Saint John the Baptist") is the capital and largest municipality on Puerto Rico. , USA; ([paragraph]) ([paragraph]) Universidad del Zulia, Maracaibo, Venezuela; (##) Ministerio de Salud Insalud, Carabobo, Venezuela; *** Centers for Disease Control and Prevention, Fort Collins, Colorado The City of Fort Collins, a home rule municipality situated on the Cache la Poudre River along the Colorado Front Range, is the county seat and most populous city in Larimer County, Colorado. , USA; and ([dagger]) ([dagger]) ([dagger]) Harvard School of Public Health The Harvard School of Public Health is (colloquially, HSPH) is one of the professional graduate schools of Harvard University. Located in Longwood Area of the Boston, Massachusetts neighborhood of Mission Hill, next to Harvard Medical School and Cambridge, Massachusetts, , Boston, Massachusetts, USA
(1.) Hayes EB, Komar N, Nasci RS, Montgomery SP, O'Leary DR. Campbell GL Epidemiology and transmission dynamics of West Nile virus disease. Emerg Infect Dis. 2005;11:1167-73.
(2.) Komar N, Clark GG. West Nile virus activity in Latin America and the Caribbean. Rev Panam Salud Publica. 2006; 19:112-7.
(3.) Morales-Betoulle ME, Morales H, Blitvich BJ, Powers AM, Davis EA, Klein R, et al. West Nile virus in horses, Guatemala. Emerg Infect Dis. 2006;12:1038-9.
(4.) Mattar S, Edwards E, Laguado J, Gonzalez M. Alvarez J, Komar N. West Nile virus antibodies in Colombian horses. Emerg Infect Dis. 2005; 11: 1497-8.
(5.) Morales MA, Barrandeguy M, Fabbri C, Garcia GB, Vissani A, Trono K, et al. West Nile virus isolation from equines in Argentina, 2006. Emerg Infect Dis. 2006;12:1559-61.
(6.) Ebel GD, Dupuis AP II, Nicholas D, Young D. Maffei J, Kramer LD. Detection by enzyme-linked immunosorbent assay enzyme-linked immunosorbent assay
Enzyme-linked immunosorbent assay (ELISA)
A diagnostic blood test used to screen patients for AIDS or other viruses. of antibodies to West Nile virus in birds. Emerg Infect Dis. 2002;8:979-82.
(7.) Dupuis AP II, Marra PP, Kramer LD. Serologic evidence of West Nile virus transmission, Jamaica, West Indies. Emerg Infect Dis. 2003;9:860-3.
(8.) Figueiredo LT. The Brazilian flaviviruses. Microbes Infect. 2000;2:1643-9.
(9.) Kuno G, Chang GJ, Tsuchiya KR, Karabatsos N, Cropp CB. Phylogeny of the genus Flavivirus. J Virol. 1998;72:73-83.
(10.) Calisher CH, Monath TP. Karabatsos N, Trent DW. Arbovirus subtyping: applications to epidemiologic studies, availability of reagents, and testing services. Am J Epidemiol. 1981;114:619-31.
Address for correspondence: Irene Bosch, Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655, USA: email: Irene. firstname.lastname@example.org