West Nile virus, Venezuela.
Serum samples from birds and horses from 33 locations in Venezuela (Online Appendix Table, available from http://www.cdc.gov/EID/content/13/4/651-appT.htm) were screened for immunoglobulin G (IgG) antibodies against WNV antigen by ELISA (6) and confirmed by plaque reduction neutralization test (PRNT) as previously described (7). The flavivirus generating the IgG response was identified by using the following criteria: 90% inhibition of virus in serum diluted at least 1:40 and 4-fold greater neutralizing antibody titer compared with closely related flaviviruses. IgG antibody against flavivirus was detected by ELISA in 14 of 576 resident birds, including 5 Turdus leucomelas, 3 Gallus gallus (captive), 2 Campylorhamphus trochilirostris, and 1 each of Elaenia flavogaster, Coereba flaveola, Thraupis palmarum, and Anisognathus flavinucha.
WNV was confirmed as the etiologic agent of infection in 5 adult birds (3 T. leucomelas [pale-breasted thrush], 1 C. flaveola [bananaquit], and 1 G. gallus [domestic chicken] with the earliest collection date in February 2006); virus neutralization titers ranged from 80 to 320. One serum sample cross-reacted with other flaviviruses tested, with equivalent titers to WNV, Saint Louis encephalitis virus (SLEV), and Ilheus virus (ILHV) and was thus considered infected with an undetermined flavivirus. Seven serum samples were negative (antibody titers <20), and 1 sample was not tested because of insufficient sample volume.
Antibody against flavivirus was detected by ELISA in 141 of 791 horses, and 34 (4.3%) were confirmed positive for WNV infection by PRNT; viral titers [greater than or equal to] 640 occurred in half of these horses. The earliest collection date for a WNV-positive horse was February 2004 and the most recent was May 2006. Specific WNV-reactive equine serum samples were distributed in valley regions (prevalence 1.3%), savannah grasslands (2.4%), the western region of Zulia (0.4%) and the Central Lake Basin (0.3%). A total of 46 (5.8%) equine serum samples were positive for neutralizing antibody to SLEV, and 8 (1.0%) samples were positive for neutralizing antibodies to ILHV. Forty-nine samples neutralized at least 2 of the 3 viruses and were classified as undetermined flaviviruses. Serum samples from 2 horses were negative in neutralization assays; 2 others were not tested because of insufficient sample volume.
Detection of WNV-infected resident birds provides strong evidence of the establishment, rather than importation, of WNV in South America. We hypothesize that ornithophilic mosquitoes (such as some Culex spp.), which are present in the area in consistently high numbers, acquired the virus through hematophagous feeding on recently infected, migrating birds. Once introduced to local mosquitoes, virus is amplified among susceptible resident birds fed upon by ornithophilic mosquitoes. This pattern allows perpetuation and subsequent establishment of virus in a continuous transmission cycle, as opposed to infection of dead-end hosts, e.g., horses. This is the first report of WNV infection in South American birds and definitive establishment of the virus in South America.
We observed varying WNV seroprevalence rates in birds and horses across regions in Venezuela (Figure). These differences reflect the focal and stochastic nature of arbovirus transmission, which depends upon many ecologic factors. One possible explanation for the greater seroprevalence in the central and eastern llanos (savannahs) and valley regions, compared with the coastal western region of Zulia State (p<0.0001, by Pearson's [chi square] test) would be virus introduction by migrating birds by an eastern migration route.
Existence of several closely related flaviviruses in the American tropics (8-10) may convey cross-protection in animals (e.g., ILHV and SLEV) or humans (dengue viruses, yellow fever virus), thereby potentially diminishing disease caused by a newly introduced flavivirus such as WNV. Although ILHV infection has not been detected in Venezuela, this flavivirus is prevalent in Brazil, Peru, French Guyana, Trinidad, and Colombia. Our study demonstrated widespread distribution of ILHV in Venezuela. Other South American flaviviruses, such as Bussuquara, Cacipacore, and Iguape, and as yet undiscovered viruses may also circulate in Venezuela.
We encourage those involved in the public and animal health systems in Venezuela to consider zoonotic flaviviruses in the differential diagnoses of human and equine cases of encephalitis and to consider ecologic surveillance for zoonotic flaviviruses in mosquito and vertebrate host populations. We recommend monitoring blood and organ donations for flavivirus infections. Our study sheds light on flavivirus distribution in Venezuela. However, nothing else is known about the ecology of zoonotic flaviviruses in this country. Such knowledge will be essential for designing effective surveillance and control should these viruses be shown to cause human illnesses.
We dedicate this work to the late Andrew Spielman, our coauthor and beloved mentor. We thank J. Rivero, I. Perez, M. Mendez, I. Matheus, M. Aguiar de Bracho, I. Carreno, J.M. Hernandez, A. Nagy, A. Suarez, N. Moncada, M. Kilpatrick, E. Rodriguez, E. Marquez, E. Marian, B. Hernandez; C. Rivero-Blanco, M. Azar, J. Rodriguez, H. Montanez, F. Alfonzo, and G. Rangel for their contributions to this study; and the Centro de Investigaciones Biomedicas, Universidad de Carabobo and the Cell Culture Core of Wadsworth Center, New York State Health Department, for their support. We also thank ProFauna and the National Institute of Parks in Venezuela for permission to obtain mosquito and bird samples.
This study was supported by grant AI45440 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, and an International Collaborations in Infectious Disease Research opportunity pool grant.
Irene Bosch, * Flor Herrera, ([dagger]) Juan-Carlos Navarro, ([double dagger]) Miguel Lentino, ([section]) Alan Dupuis, ([paragraph]) (#) Joseph Maffei, ([paragraph]) (#) Matthew Jones, ([paragraph]) (#) Ernesto Fernandez, ** Nelson Perez, ([dagger])([dagger]) Jorge Perez-Eman, ([double dagger]) Anthony Erico Guimaraes, ([double dagger]) ([double dagger]) Roberto Barrera, ([subsection]) Nereida Valero, ([paragraph]) ([paragraph]) Johanny Ruiz, ([dagger]) Glenda Velasquez, (##) Juan Martinez, ([double dagger]) Guillermo Comach, ([dagger]) Nicholas Komar, *** Andrew Spielman, ([dagger]) ([dagger]) ([dagger]) (1) and Laura Kramer ([paragraph]) (#)
* University of Massachusetts Medical School, Worcester, Massachusetts, USA; ([dagger]) Universidad de Carabobo Biomed, Maracay, Venezuela; ([double dagger]) Universidad Central de Venezuela, Caracas, Venezuela; ([section]) Coleccion Omitologica Phelps, Caracas, Venezuela; ([paragraph]) New York State Department of Health, Albany, New York, USA; #State University of New York at Albany, Albany, New York, USA; ** Universidad Central de Venezuela, Maracay, Venezuela; ([dagger]) ([dagger]) Instituto Nacional de Investigaciones Agricolas, Maracay, Venezuela; ([double dagger]) ([double dagger]) Instituto Oswaldo Cruz, Rio de Janeiro, Brazil; ([subsection]) Centers for Disease Control and Prevention, San Juan, Puerto Rico, USA; ([paragraph]) ([paragraph]) Universidad del Zulia, Maracaibo, Venezuela; (##) Ministerio de Salud Insalud, Carabobo, Venezuela; *** Centers for Disease Control and Prevention, Fort Collins, Colorado, USA; and ([dagger]) ([dagger]) ([dagger]) Harvard School of Public Health, Boston, Massachusetts, USA
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Address for correspondence: Irene Bosch, Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655, USA: email: Irene. firstname.lastname@example.org