Water manganese exposure and children's intellectual function in Araihazar, Bangladesh.
Exposure to manganese via inhalation has long been known to elicit neurotoxicity neurotoxicity /neu·ro·tox·ic·i·ty/ (noor?o-tok-sis´it-e) the quality of exerting a destructive or poisonous effect upon nerve tissue. in adults, but little is known about possible consequences of exposure via drinking water drinking water
supply of water available to animals for drinking supplied via nipples, in troughs, dams, ponds and larger natural water sources; an insufficient supply leads to dehydration; it can be the source of infection, e.g. leptospirosis, salmonellosis, or of poisoning, e.g. . In this study, we report results of a cross-sectional investigation of intellectual function in 142 10-year-old children in Araihazar, Bangladesh, who had been consuming tube-well water with an average concentration of 793 [micro]g Mn/L and 3 [micro]g arsenic/L. Children and mothers came to our field clinic, where children received a medical examination in which weight, height, and head circumference were measured. Children's intellectual fimction was assessed on tests drawn from the Wechsler Intelligence Scale for Children Wechsler intelligence scale for children
A standardized intelligence test that is used for assessing children from 5 to 15 years old. , version III, by summing weighted items across domains to create Verbal, Performance, and Full-Scale raw scores. Children provided urine specimens for measuring urinary As and creatinine creatinine /cre·at·i·nine/ (kre-at´i-nin) an anhydride of creatine, the end product of phosphocreatine metabolism; measurements of its rate of urinary excretion are used as diagnostic indicators of kidney function and muscle mass. and were asked to provide blood samples for measuring blood lead, As, Mn, and hemoglobin concentrations. After adjustment for sociodemographic covariates, water Mn was associated with reduced Full-Scale, Performance, and Verbal raw scores, in a dose-response fashion; the low level of As in water had no effect. In the United States United States, officially United States of America, republic (2005 est. pop. 295,734,000), 3,539,227 sq mi (9,166,598 sq km), North America. The United States is the world's third largest country in population and the fourth largest country in area. , roughly 6% of domestic household wells have Mn concentrations that exceed 300 lag Mn/L, the current U.S. Environmental Protection Agency Environmental Protection Agency (EPA), independent agency of the U.S. government, with headquarters in Washington, D.C. It was established in 1970 to reduce and control air and water pollution, noise pollution, and radiation and to ensure the safe handling and lifetime health advisory level. We conclude that in both Bangladesh and the United States, some children are at risk for Mn-induced neurotoxicity. Key words: children, IQ, manganese. Environ Health Perspect 114:124-129 (2006). doi:10.1289/ehp.8030 available via http://dx.doi.org/ [Online 9 August 2005]
Manganese, a transition metal, is an essential nutrient An essential nutrient is a nutrient required for normal body functioning that cannot be synthesized by the body and must be obtained from a dietary source. Some categories of essential nutrient include vitamins, dietary minerals, essential fatty acids, and essential amino acids. in humans and animals. Like many other essential metals, excessive exposure has been associated with adverse health effects, in this case, neurotoxicity. Mn deficiency is rare in humans because the element is ubiquitous in common foods. Typically, dietary Mn intake greatly exceeds that from drinking water; exposure from water is usually small. Nevertheless, the World Health Organization (WHO) has established a health-based water Mn (WMn) standard of 500 [micro]g/L (WHO 2002). Similarly, the U.S. Environmental Protection Agency (EPA EPA eicosapentaenoic acid.
n.pr See acid, eicosapentaenoic.
n. ) recently issued a drinking water health advisory for Mn that yielded a lifetime health advisory value of 300 [micro]g/L in drinking water (U.S. EPA 2004).
Although the evidence linking inhalation exposure to neurotoxicity is compelling, evidence that links Mn exposure from drinking water to adverse neurologic effects is unconvincing. Occupational inhalation exposures in adults (Gorell et al. 1999; Mena et al. 1967; Takeda 2003) have repeatedly been associated with neuromotor consequences--specifically, akinetic-rigid Parkinsonism--largely due to toxic effects on dopaminergic neurons of the basal ganglia basal ganglia
1. The caudate and lentiform nuclei of the brain and the cell groups associated with them, considered as a group.
2. All of the large masses of gray matter at the base of the cerebral hemisphere. . Very little research has examined the consequences of excessive Mn exposure on neurologic or developmental functioning in children.
Last year, from a cross-sectional study cross-sectional study
See synchronic study.
n the scientific method for the analysis of data gathered from two or more samples at one point in time. of 201 10-year-old children in Bangladesh, we concluded that water arsenic (WAs) exposure was adversely associated with intellectual function (Wasserman et al. 2004). In the same study, we observed a moderate and statistically significant positive association between WAs and WMn (Spearman spear·man
A man, especially a soldier, armed with a spear. r = 0.39, p < 0.0001). Roughly 75% of the wells in our study region contained As in excess of the maximum comaminant level of 10 [micro]g/L (van Geen et al. 2003), and 80% had levels of Mn that exceeded the WHO standard of 500 [micro]g/L (Cheng et al. 2004). Among those 201 children, well WMn was adversely associated with children's intellectual functioning; however, the magnitude of the association was reduced by approximately half (and was no longer statistically significant) when we adjusted for WAS. It was thus undear whether or not WMn had an independent association with intellectual functioning. To facilitate interpretation, we sought an additional sample of children with low WAs.
Thus, to test the hypothesis that Mn might have an independent adverse effect on cognitive function cognitive function Neurology Any mental process that involves symbolic operations–eg, perception, memory, creation of imagery, and thinking; CFs encompasses awareness and capacity for judgment , we returned to our Bangladesh study region. Our original As study included 54 children using wells with low As concentrations, that is, [less than or equal to] 10 [micro]g/L. In our subsequent fieldwork, we recruited 88 additional children drinking from wells comparably low in As.
Here we report on associations between WMn and intellectual function in the combined sample of 142 children.
Materials and Methods
Our present project is part of a larger ongoing multidisciplinary study by health, earth, and social scientists working collaboratively in Araihazar, Bangladesh. The study was approved by the Bangladesh Medical Research Council and the Columbia University Medical Center Columbia University Medical Center is the name of the medical complex associated with Columbia University, and covers several blocks (primarily between 165th and 168th Streets from the Henry Hudson Parkway to Audubon Avenue) in the Washington Heights section of Manhattan. institutional review boards. We have previously described the region and the larger cohort study A cohort study is a form of longitudinal study used in medicine and social science. It is one type of study design.
In medicine, it is usually undertaken to obtain evidence to try to refute the existence of a suspected association between cause and disease; failure to refute of adults, whose children are the focus of this investigation (Wasserman et al. 2004). As in most of rural Bangladesh, people in Araihazar live in houses with floors made from mud or cement, with roofs and walls constructed from concrete, tin, or straw. Members of extended families live in clusters of individual houses (a ban), surrounded by family farmland. Each bari has one or more tube wells, usually owned by a senior family member. This region is not particularly poor by Bangladeshi standards. Before conducting this study, we secured review and approval from institutional review boards at Columbia and in Bangladesh and obtained written informed consent from parents as well as child assent.
Details regarding the enrollment of the original 54 children are available in a previously published study (Wasserman et al. 2004). Briefly, in 2002, of the 11,749 adults enrolled in our cohort study, we selected 400 of their children at random (using 400 different wells) between 9.5 and 10.5 years of age; we ultimately assessed 201 children, 54 of whom were drinking from a well with WAs concentration [less than or equal to] 10 [micro]g/L and who are included in these analyses. In 2004, we identified an additional 407 children who met three inclusion criteria
Inclusion criteria are a set of conditions that must be met in order to participate in a clinical trial. : a) Their well had [less than or equal to] 10 [micro]g As/L, b) their estimated age from earlier parental interviews was 9.5-10.5 years, and c) neither they nor their siblings had participated in our previous study. We limited the sample to children who were currently attending school. Of the first 199 families visited between May and November 2004, 88 met the inclusion criteria and consented to participate. Of the remaining 108 children, 76 were not of the desired age, 8 did not attend school, 11 had relocated, 12 refused to participate, and 1 was physically disabled.
Children and their mothers came to our field clinic, where the children participated in assessments described below and received a medical examination by a study physician. Weight, height, and head circumference were measured. In addition, children provided spot urine specimens for the measurement of urinary As (UAs) and urinary creatinine (UCr) and were asked to provide a blood sample for the measurement of blood Mn (BMn), blood As (BAs), blood lead (BPb), and hemoglobin (Hgb) concentrations. Of the 142 children assessed, 95 agreed to provide blood samples. Because our original intent was to measure only BPb and BAs, Mn-free needles were not used. The anticoagulant anticoagulant (ăn'tēkōăg`yələnt), any of several substances that inhibit blood clot formation (see blood clotting). had negligible concentrations of Mn, Pb, and As. Blood and urine samples were frozen at -20[degrees]C and transported on dry ice to New York New York, state, United States
New York, Middle Atlantic state of the United States. It is bordered by Vermont, Massachusetts, Connecticut, and the Atlantic Ocean (E), New Jersey and Pennsylvania (S), Lakes Erie and Ontario and the Canadian province of . Information on family demographics (e.g., parental education, occupation, housing type) was obtained from the baseline interview of parents during their enrollment in the cohort study. Information on the primary source of drinking water was obtained from the child's mother. Parents were asked their age, education, and occupation; whether their home included a television; and the birth order of their children. As an additional surrogate for social class, the type of roofing on the well-owner's home was recorded as thatched thatch
1. Plant stalks or foliage, such as reeds or palm fronds, used for roofing.
2. Something, such as a thick growth of hair on the head, that resembles thatch.
3. Dead turf, as on a lawn.
tr.v. , tin, or cement and subsequently ranked on a scale (thatched, lowest; cement, highest). Children were given a toy as a sign of appreciation for their participation; all families participating in the larger cohort study continue to receive primary medical care at our own field clinic.
Water analyses. Water samples were collected at the onset of the cohort study as part of a survey of all wells in the study region. Field sample collection and laboratory analysis procedures are described elsewhere in detail (Cheng et al. 2004; van Geen et al. 2003, 2005). In brief, samples were collected in 60-mL acid-cleaned polyethylene bottles, and 1 mL 7 N high-purity HCl was added for preservation before being shipped to Columbia University's Lamont-Doherty Earth Observatory Lamont-Doherty Earth Observatory (LDEO) is a world-class research institution specializing in the Earth sciences and is part of Columbia University. The current director of Lamont is G. Michael Purdy. for analysis. Initially, some samples were analyzed for AS only by graphite-furnace atomic absorption spectrometry Absorption spectrometry
A scientific procedure to determine chemical makeup of samples.
Mentioned in: Herbalism, Traditional Chinese . For the present study, all samples were reanalyzed by high-resolution inductively coupled plasma mass spectrometry ICP-MS (Inductively coupled plasma mass spectrometry) is a type of mass spectrometry that is highly sensitive and capable of the determination of a range of metals and several non-metals at concentrations below one part in 1012. (HR ICP-MS ICP-MS Inductively Coupled Plasma Mass Spectroscopy ). The analytical detection limit of the method is 0.1 [micro]g/L; the standard deviation In statistics, the average amount a number varies from the average number in a series of numbers.
(statistics) standard deviation - (SD) A measure of the range of values in a set of numbers. of a single measurement is conservatively estimated at 4 [microp]g/L (van Geen et al. 2005). Mn concentrations were also determined by HR ICP-MS. The detection limit of the method is also 0.1 [micro]g/L, and its precision was 2% (Cheng et al. 2004).
Urinary measurements. UAs concentrations were assayed by graphite-furnace atomic absorption at the Mailman School of Public Health at Cokunbia University, using a Perkin-Elmer Analyst 600 system (Perkin-Elmer, Shelton, CT) as described previously (Nixon et al. 1991). Our laboratory participates in a quality-control program coordinated by P. Weber at the Quebec Toxicology Center (Sainte-Foy, Quebec, Canada). During the course of this study, intraclass correlation In statistics, the intraclass correlation (or the intraclass correlation coefficient) is a measure of correlation, consistency or conformity for a data set when it has multiple groups. coefficients between our laboratory's values and samples calibrated cal·i·brate
tr.v. cal·i·brat·ed, cal·i·brat·ing, cal·i·brates
1. To check, adjust, or determine by comparison with a standard (the graduations of a quantitative measuring instrument): at Weber's laboratory were 0.99. UAs levels were also adjusted for UCr concentrations, which were analyzed by a colorimetric col·or·im·e·ter
1. Any of various instruments used to determine or specify colors, as by comparison with spectroscopic or visual standards.
2. Sigma Diagnostics Kit (Sigma, St. Louis, MO). Blood Hgb levels were determined by standard methods.
ICP-MS blood measurements. Venous whole blood samples were analyzed for BPb, BMn, and BAs concentrations in the Trace Metal Core Laboratory at the Mailman School of Public Health, which used a Perkin-Elmer Elan DRC DRC Democratic Republic of Congo
DRC Down (Stage) Right Center
DRC Director(ate) of Reserve Components
DRC Disability Rights Commission (United Kingdom) II ICP-MS equipped with an AS 93+ autosampler. ICP-MS-DRC methods for metals in whole blood were developed according to according to
1. As stated or indicated by; on the authority of: according to historians.
2. In keeping with: according to instructions.
3. published procedures (Pruszkowski et al. 1998; Stroh 1988), with modifications for blood sample preparation as suggested by the Laboratory for ICP-MS Comparison Program (Institut National de Sante Publique du Quebec). A 3-mL EDTA EDTA: see chelating agents. Vacutainer of whole blood was thawed, thoroughly mixed, and then diluted 50 times with the following diluent diluent /dil·u·ent/ (dil´oo-int)
1. causing dilution.
2. an agent that dilutes or renders less potent or irritant.
Serving to dilute.
n. : 1% HN[O.sub.3], 0.2% Triton-X-100, 0.5% N[H.sub.4]OH. The sample was then centrifuged for 10 min at 3,500 rpm, and the supernatant supernatant /su·per·na·tant/ (-na´tant) the liquid lying above a layer of precipitated insoluble material.
the liquid lying above a layer of precipitated insoluble material. used for analysis. One multielement standard solution was used for instrument calibration. The metal concentrations of that solution were chosen to cover the expected ranges of analyte concentrations in the blood samples: 5, 25, and 50 [micro]g/L. Special attention was given to correction for matrix-induced interferences. Matrix suppression is compensated very well by the selection of suitable internal standards (IS), which are matched to masses and, if possible, to ionization ionization: see ion.
Process by which electrically neutral atoms or molecules are converted to electrically charged atoms or molecules (ions) by the removal or addition of negatively charged electrons. properties of the analytes. For AS, we used iridium iridium (ĭrĭd`ēəm), metallic chemical element; symbol Ir; at. no. 77; at. wt. 192.22; m.p. about 2,410°C;; b.p. about 4,130°C;; sp. gr. 22.55 at 20°C;; valence +3 or +4. (Ir); for Pb and Mn, we used lutetium lutetium, formerly lutecium (both: ltē`shēəm), metallic chemical element; symbol Lu; atomic number 71; at. wt. 174.967; m.p. about 1,663°C;; b.p. (Lu) and gallium (Ga), respectively. A stock IS spiking solution was prepared that ultimately delivered to each tube 10 ng of Lu and Ir, and 100 ng Ga. After calibrating the instrument, we ran quality control samples, that is, blood samples with known analyte concentrations obtained from the Laboratory for ICP-MS Comparison Program.
Quality-control blood samples were purchased to cover the range of concentrations of analytes of interest and were run during the course of each day. Over a period of 1 month, during which all of these study samples were analyzed, the intraprecision coefficients of variation for BPb, BMn, and BAs were 1.5, 4.0, and 4.3%, respectively. In late 2004, when these samples were run, we also joined the Quebec Multi-Elements External Quality Assessment Scheme run by the Laboratory for ICP-MS Comparison Program. Three times per year, that lab sends blood urine and serum samples with known concentrations of 23 elements. Only one blood sample was received and analyzed during the course of this study, but our reported concentrations for BPb, BMn, and BAs were well within the expected target ranges.
Children's intellectual function. The Wechsler Intelligence Scale for Children, version III (WISC-III WISC-III Wechsler Intelligence Scales for Children, 3rd Edition ) (Wechsler 1991), suitable for children [greater than or equal to] 6 years of age, consists of five (or six, depending on administration) verbal subtests that together provide a Verbal IQ score, and a similar number of performance subtests that together provide a Performance IQ. Neither the WISC-III (Wechsler 1991) nor any other recently well-standardized child IQ test has been adapted or standardized for use in Bangladesh.
In Araihazar, living conditions living conditions npl → condiciones fpl de vida
living conditions npl → conditions fpl de vie
living conditions living differ dramatically from those in the Western settings where this test was developed, which necessitated adaptations of the test for use in this culture. For example, a typical Araihazar home consists of a single room, often with a dirt floor. Most families use biomass fuel (leaves, hay, dung) for cooking. Electricity is available in most homes, typically consisting of one or two bulbs used for lighting. Many common Western household items, such as telephones and bathtubs, are rare.
We have previously described our adaptations of the WISC-III for this population (Wasserman et al. 2004). In short, we used six subtests that seemed the most culturally adaptable to this cultural context. Of the WISC-III Verbal subtests, we used Similarities and Digit Span; of the Performance subtests, we used Picture Completion, Coding, Block Design, and Mazes. As noted previously (Wasserman et al. 2004), we summed items across Verbal, Performance, and Full-Scale domains to create Verbal, Performance, and Full-Scale raw scores and also transformed these into measures of estimated Verbal, Performance, and Full-Scale IQ using procedures presented in the test manual (Wechsler 1991), despite the obvious limitations in application to this population.
Maternal intelligence was assessed with Raven's Standard Progressive Matrices, a non-verbal test relatively free of cultural influences (Raven et al. 1983).
Translation and Training
All tests and interviews were translated (and back-translated) between Bangla (Bengali) and English. As noted above, items deemed to be culturally inappropriate were altered or omitted. Materials were piloted to ensure maternal and child comprehension. Subsequently, two interviewers were trained by a competent tester (G.A.W.) and continued with supervised practice sessions for 2 weeks. All written test responses were rechecked when data were sent to Columbia University Columbia University, mainly in New York City; founded 1754 as King's College by grant of King George II; first college in New York City, fifth oldest in the United States; one of the eight Ivy League institutions. for entry.
Outcomes. Because of concerns regarding the application of U.S. standardization of the WISC-III to Bangladeshi children, we first conducted analyses that predicted Verbal, Performance, and Full-Scale raw scores. Because the psychometric psy·cho·met·rics
n. (used with a sing. verb)
The branch of psychology that deals with the design, administration, and interpretation of quantitative tests for the measurement of psychological variables such as intelligence, aptitude, and properties of IQ scores are more familiar to readers, we also applied the same analytical models to the prediction of estimated Verbal IQ, Performance IQ, and Full-Scale IQ.
Covariate adjustment and missing data.
We adjusted our models for the same set of covariates described in our previous As study (Wasserman et al. 2004): maternal education (categorized as none, 1-5 years, and 6-13 years) and intelligence, house type (thatched roof or poorer, corrugated cor·ru·gate
v. cor·ru·gat·ed, cor·ru·gat·ing, cor·ru·gates
To shape into folds or parallel and alternating ridges and grooves.
v.intr. tin, concrete construction), family ownership of a television, and child height and head circumference. For one girl without height data, we substituted the mean height for other participating girls.
Analytical model. Analyses first estimated differences in the three measures of intellectual function, based only on the sociodemographic maternal factors, using linear regression Linear regression
A statistical technique for fitting a straight line to a set of data points. models. We then estimated the incremental association of exposures (WMn, UAs, and WAs) singly and in combination, measured continuously. We repeated our analyses, categorizing children into groups based on quartiles of WMn to illustrate dose-response relationships. To examine further the dose-response relationship between WMn and intellectual function, we subsequently stratified stratified /strat·i·fied/ (strat´i-fid) formed or arranged in layers.
Arranged in the form of layers or strata. children into four approximately equal-sized groups, based on well WMn. Because results based on quartiles of exposure were similar to those based on cut-points used in policy statements, we present data arrayed by the cut points, which correspond to various policy guidelines: Group 1 (reference), Mn < 200 [micro]g/L (n = 38; 27%); group 2, 200 [greater than or equal to] Mn < 500 [micro]g/L (n = 45; 32%); group 3, 500 [greater than or equal to] Mn < 1,000 [micro]g/L (n = 31; 22%); group 4, Mn [greater than or equal to] 1,000 [micro]g/L (n = 28; 20%).
We next repeated these analyses for the subset of 95 children who provided blood samples for BMn, BPb, and BAs, measured continuously. The following variables were log-transformed to normalize normalize
to convert a set of data by, for example, converting them to logarithms or reciprocals so that their previous non-normal distribution is converted to a normal one. their distributions: BPb, BAs, BMn, UAs, UCr, WMn, and WAs.
For the most part, analyses are based on n = 142 children; however, those considering BPb, BAs, and BMn are based on n = 95 children.
Table 1 presents descriptive information for all demographic, water, and biochemical variables. Average child age was 10 years; approximately half of the sample were male; roughly one-third of children had regular access to a television, and > 70% lived in a house with a tin roof. On average, mothers and fathers reported 3.1 and 3.9 years of education, respectively. Children providing blood samples did not differ on any measure of exposure or intellectual function or on sociodemographic characteristics from those not providing blood samples (data not shown).
The mean WMn concentration was 795 [micro]g/L, with a very wide range, from 4 to 3,908 [micro]g/L. By design, the range of WAs concentrations was narrow (0.1-10 [micro]g/L), with a mean of 3.0 [micro]g/L. Table 2 presents a matrix of Spearman correlation coefficients among water, urine, and blood metal measurements. Despite the restricted range of As exposure in the present sample, correlations among measurements of As in water, urine, and blood were all significantly positive. WMn was also positively correlated with WAs and BAs, but not so highly correlated as to preclude examination of their independent effects on child intelligence. WMn was not associated with BMn.
To obtain rough estimates of Mn intake from drinking water, we first calculated the mean WMn concentration for each quartile Quartile
A statistical term describing a division of observations into four defined intervals based upon the values of the data and how they compare to the entire set of observations.
Each quartile contains 25% of the total observations. of WMn. The means for the four quartiles were 103,440, 801, and 1,923 [micro]g Mn/L. Based on a recent U.S. Institute of Medicine (IOM IOM
See: Index and Option Market ) report (IOM Food and Nutrition Food and Nutrition
See also cheese; dining; milk.
Rare. the act or habit of reclining at meals.
Medicine. thescience of nutrition.
Pathology. Board 2004), we estimated daily water intake for 10-year-old boys and girls boys and girls
mercurialisannua. to be 2.4 and 2.1 L/day, respectively. For the four quartiles, the product of WMn concentration times daily water intake yielded estimates of daily Mn consumption (from water only) of 0.25, 1.06, 1.92, and 4.37 mg/day for boys and 0.21, 0.93, 1.68, and 3.82 mg/day for girls.
Relationship between Covariates and Intellectual Function
Linear regression analyses, predicting test raw scores from the sociodemographic features retained in the final "core" model, revealed better scores among children who a) had more educated mothers; b) lived in more adequate dwellings; c) had access to television; d) were taller; and e) had a larger head circumference (data not shown). Collectively, these factors explained 25.0, 24.1, and 17.7% of the variances in Full-Scale, Performance, and Verbal raw scores, respectively.
Relationship between Well WMn and Intellectual Function
As Table 3 shows, before adjustment for sociodemographic factors, WMn was significantly associated with Full-Scale, Performance, and Verbal raw scores (B-values = -5.20, -4.43, and -0.80; p-values < 0.001, 0.001, and 0.02, respectively), explaining 10, 10, and 4%, respectively, of the variances in scores. After adjusting for sociodemographic factors, WMn concentration remained significantly and negatively associated with all three scores (B-values = -4.35, -3.76, and -0.63; p-values < 0.001, 0.001, and 0.05, respectively) and also explained incremental portions of the variances in scores (6.3, 6.9, and 2.3%, respectively). The addition of WMn to the core regression models produced negligible changes in the associations between core model variables and intellectual function raw scores. All results were similar when "IQ" outcomes were substituted for raw scores (data not shown).
Controlling for As Exposure
The addition of WAs to these regression models failed to change the pattern of associations between intellectual function and sociodemographic variables, or between intellectual function and WMn. Not surprisingly, given that we sampled only children with low levels of WAs, WAs was not significantly associated with intellectual function. Similarly, associations between WMn and intellectual function scores were unchanged when we adjusted for both UAs and UCr. Neither UAs nor UCr was associated with children's intellectual function (data not shown).
Dose-Response Relationships between Well WMn and Intellectual Function
To examine the dose-response relationship between WMn and intellectual function, we subsequently stratified children into four approximately equal-sized groups, based on well WMn.
Before adjustment. Unadjusted for other contributors, children in group 1, compared with those in the other three groups with higher WMn, had higher Full-Scale scores: groups 2 and 4 were significantly different from group 1 (B-values = -11.93 and -23.80; p-values < 0.05 and 0.0001, respectively), whereas the finding for group 3 was in the same direction but did not achieve significance (B = -8.92, p = 0.09). Similarly, compared with children in group 1, children in groups 2, 3, and 4 had lower Performance scores (B-values = -10.42, -7.97, and -20.39; p-values < 0.05, 0.07, and 0.0001, respectively). Finally, compared with children in group 1, those in group 4 also had significantly poorer Verbal scores (B = -3.76, p < 0.005); children in groups 2 and 3 had poorer Verbal scores than did those in group 1, but not significantly so.
After adjustment. Figure 1 illustrates the adjusted Full-Scale, Performance, and Verbal raw scores by WMn group. After adjustment for other factors, children in groups 1 and 4 were significantly different for Full-Scale, Performance, and Verbal scores (B-values = -21.28, -18.43, and -3.19; p-values < 0.0001, 0.0001, and 0.02, respectively). Compared with group 1, children in groups 2 and 3 had lower, albeit not significantly so, Full-Scale (B-values = -8.57 and -7.90, respectively; p-values < 0.10) and Performance scores (B-values = -7.79 and -7.34, respectively; p-values [less than or equal to] 0.07). Verbal score comparisons between children in groups 2 and 3 and those in group 1 were in the expected direction but did not approach significance.
Relations considering BPb, BAs, BMn, and intellectual function. For the 95 children with blood samples, we examined the relations of BPb, BAs, and BMn to intellectual function, again adjusting for the same demographic features. When all three blood measures were added to the core model, only BPb was related to intellectual function (data not shown). In subsequent analyses that simultaneously considered WMn, WAs, and BPb, the adverse associations between WMn and Full-Scale and Performance scores persisted after adjustments (B-values = -4.56 and -3.82; p-values < 0.01).
This study indicates that exposure to Mn in drinking water is associated with neurotoxic neurotoxic
pertaining to or emanating from a neurotoxin.
a case of poisoning by a neurotoxin.
neurotoxic adjective effects in children. In our previous cross-sectional study of 10-year-old children in Araihazar, which reported an adverse association between WAs and child intellectual function, the mean WAs concentration was 118 [micro]g/L, and the mean WMn concentration was 1,386 [micro]g/L. In that study, before adjustment for WAs, WMn was adversely associated with children's intellectual function, but the association did not persist once WAS was added to the regression model. The present study, however, was specifically designed to examine possible effects of WMn in the absence of confounding confounding
when the effects of two, or more, processes on results cannot be separated, the results are said to be confounded, a cause of bias in disease studies.
confounding factor by WAs. WAs was controlled by limiting the sample to children drinking from wells with As < 10 [micro]g/L (with a mean of 3 [micro]g/L), whereas WMn was free to vary. The lower mean Mn concentration of well water compared with the previous study (793 vs. 1,386 [micro]g/L) reflects the fact that a significant fraction of the wells selected on the basis of their low As content tap older and deeper Bangladesh aquifers that are generally lower in Mn. This is the case not only in our study area but throughout the country (British Geological Survey The British Geological Survey (BGS) is a partly publicly-funded body which aims to advance geoscientific knowledge of the United Kingdom landmass and its continental shelf by means of systematic surveying, monitoring and research. and Department of Public Health and Engineering 2001; Cheng et al., 2004). Although the concentrations of the two elements were correlated (Spearman r = 0.36), exposure to As, as measured by water, urine, and blood concentrations, was essentially negligible, and none of the measures of AS exposure was associated with any measure of intellectual function. In neither study have we detected a significant interaction between As and Mn exposure in relation to intellectual function; however, we lack adequate statistical power to definitively address this possibility.
The neurotoxicity of Mn in adults with occupational inhalation exposure is well established (Agency for Toxic Substances and Disease Registry The United States Agency for Toxic Substances and Disease Registry, (ATSDR) is an agency for the U.S. Department of Health and Human Services that is directed by a congressional mandate to perform specific functions concerning the effect on public health of hazardous 2001; Cook et al. 1974; Rods et al. 1999). The syndrome known as "manganism" is characterized by a Parkinson-like condition with weakness, anorexia, apathy, slowed speech, emotionless e·mo·tion·less
Devoid of emotion; impassive.
Adj. 1. facial expression facial expression,
n the use of the facial muscles to communicate or to convey mood. , and slow movement of the limbs. In contrast, findings from studies of environmental exposures to Mn are limited (Hudnell 1999; Mergler 1999; Mergler and Baldwin 1997). An epidemiologic investigation in Greece examined possible correlations between long-term (> 10 years) Mn exposure from drinking water and neurologic effects in a random sample of an elderly population (Kondakis et al. 1985); WMn concentrations ranged from 4 to 2,300 [micro]g/L. Composite neurologic scores (including weakness/fatigue, gait disturbances, tremors) differed significantly between highly exposed and control populations. Two available studies of environmental Mn exposure in children have focused on motor functioning (He et al. 1994, as cited in Mergler 1999; Takser et al. 2003).
Among Parisian children followed from birth through their preschool years (Takser et al. 2003), after adjustment for sex and maternal education, cord BMn levels were negatively associated with scores on three scales derived by the authors from the McCarthy Scales (McCarthy 1972): attention, nonverbal memory, and hand skill. Among children 11-13 years of age, a comparison (unadjusted) of those from an area with high levels of Mn sewage irrigation irrigation, in agriculture, artificial watering of the land. Although used chiefly in regions with annual rainfall of less than 20 in. (51 cm), it is also used in wetter areas to grow certain crops, e.g., rice. with those from a control area revealed lower scores on tests of short-term memory short-term memory
Abbr. STM The phase of the memory process in which stimuli that have been recognized and registered are stored briefly. , manual dexterity, and visuoperceptive speed in exposed children (He et al. 1994, as cited in Mergler 1999).
Mn is an essential element that is required by enzymes such as Mn superoxide dismutase superoxide dismutase
An enzyme that catalyzes the decomposition of a superoxide into hydrogen peroxide and oxygen.
superoxide dismutase and pyruvate carboxylase Pyruvate carboxylase is an enzyme of the ligase class that catalyzes the irreversible carboxylation of pyruvate to form oxaloacetate. and serves to activate certain kinases, transferases, and other enzymes (WHO 2002). Substantial amounts are obtained in the diet, and deficiency is extremely rare. The first reported case of deficiency occurred in a man fed a chemically defined diet (as part of a study of vitamin K vitamin K
Any of several fat-soluble compounds essential for the clotting of blood. A deficiency of vitamin K in the body leads to an increase in clotting time. In 1929 a previously unrecognized fat-soluble substance present in green leafy vegetables was found to be required requirements) in which Mn was inadvertently left out (Doisy 1973). The IOM has determined the total adequate intake adequate intake (AI),
n the consumption and absorption of sufficient food, vitamins, and essential minerals necessary to maintain health. See also dietary reference intakes; estimated average requirement; recommended dietary allowances; and upper intake values for Mn for boys and girls, 9-13 years of age (the age group of interest to the present study) to be 1.9 and 1.6 mg/day, respectively (IOM Food and Nutrition Board 2002). One would therefore expect the shape of the dose-response relationship between WMn and cognitive function to be complex and dependent on dietary intake. We made no attempt m estimate dietary intake in this study of 10-year-old children and therefore cannot comment as to whether the WHO and U.S. EPA drinking water standards of 500 [micro]g/L and 300 [micro]g/L, respectively, are protective of the health of children.
Based on a comprehensive review of the literature, a risk assessment carried out by the IOM (IOM Food and Nutrition Board 2002) has generated age-dependent estimates of the tolerable upper intake level (UL), which is defined as the highest daily total dose of Mn that is likely to pose no risk of adverse health effects in almost all people. The UL for children 9-13 years of age was estimated to be 6 mg/day. In the present study, our estimates of water-borne Mn intake (by quartile of WMn) were 0.25, 1.06, 1.92, and 4.27 mg/day for boys, and 0.21, 0.93, 1.68, and 3.82 rag/day for girls. Obviously, none of these values exceed the UL of 6 rag/day, although additional dietary exposure could have pushed the total daily dose above that value. However, both the valence state and the bioavailability bioavailability /bio·avail·a·bil·i·ty/ (bi?o-ah-val?ah-bil´i-te) the degree to which a drug or other substance becomes available to the target tissue after administration.
n. of Mn in food (oxidized oxidized
having been modified by the process of oxidation.
see absorbable cellulose. Mn) and water (reduced Mn) differ, and these factors may contribute to the observed neurotoxicity of Mn from drinking water. The bioavailability of dietary Mn is very low, with estimates ranging from roughly 1 to 5% (IOM Food and Nutrition Board 2002); absorption is increased in the presence of iron deficiency iron deficiency A relative or absolute deficiency of iron which may be due to chelation in the GI tract, loss due to acute or chronic hemorrhage or dietary insufficiency Sources Meat, poultry, eggs, vegetables, cereals, especially if fortified with iron; per the (Finley 1999) and impaired by calcium supplementation calcium supplementation Metabolism The addition of Ca2+ to the diet, usually in the form of calcium carbonate (Freeland-Graves and Lin 1991). In contrast, it has been estimated that compared with that for food, the bioavailability of Mn from drinking water is 1.4 times greater in nonfasted subjects, and two times greater in fasted subjects (Ruoff 1995, cited in U.S. EPA 1999). Thus, dose for dose, waterborne Mn is likely more toxic than dietary Mn.
We found no evidence of a relationship between BMn and any measure of child intellectual function. This is not surprising because the use of blood levels as a means of evaluating occupational exposure to Mn has also been disappointing (National Research Council 1973). Our failure to use Mn-free needles may have introduced noise into the measurement of BMn and may explain the absence of an observed association between BMn and child intellectual functioning. As mentioned above, others (Takser et al. 2003) have observed a relationship between cord BMn and McCarthy scores at age 3. Because Mn is transported in the blood on transferrin transferrin /trans·fer·rin/ (-fer´in) a glycoprotein mainly produced in the liver, binding and transporting iron, closely related to the apoferritin of the intestinal mucosa.
n. , we speculate that serum Mn levels might be a better biomarker of exposure. Indeed, several reports indicate that serum or plasma Mn concentrations vary with dietary Mn intake (Davis and Greger 1992; Freeland-Graves and Turnlund 1996).
Limitations. We cannot comfortably make a statement about IQ points lost in relationship to WMn exposure, because we cannot apply U.S. standardization norms to generate IQ scores in the present study population. As we have previously pointed out, the lack of measures of intelligence, standardized for use in Bangladesh, hampers our ability to draw inferences about IQ points lost at given levels of exposure. Although we have followed sound procedures for adapting a widely used instrument to this very different cultural setting, and although we have avoided, for the most part, drawing conclusions about IQ, the measures used here are not measures of IQ, and the absence of standardized measures remains a limitation.
Our use of raw scores avoids pitfalls that would result from using nonstandardized procedures, but the removal of culturally bound items and subscales diverges from common practice. Nevertheless, the fact that other predictors of child intellectual function, such as maternal education and child height and head circumference, were significantly related to intellectual raw scores in the expected directions gives us confidence in the validity of the observed associations with Mn.
To date, we have studied only 10-year-old children, and we do not know if the observed deficits can be detected earlier in life. It is interesting to note that although breast milk contains between 3 and 10 lag Mn/L (Agency for Toxic Substances and Disease Registry 2001), infant formulas (Collipp et al. 1983) have been reported to contain as much as 50 to 300 [micro]g/L. Our findings, coupled with the absence of reports of Mn deficiency in young children, led us to conclude that the possible consequences in children of excess exposure to Mn from water, diet, and gasoline additives (Kaiser 2003) deserve further attention.
We did not measure Mn in food or in air and thus could not estimate total Mn exposure. On the other hand, the impact of the absence of these exposure inputs would actually bias our findings toward the null. The fact that we observe a relationship between WMn and child intellectual function in the absence of estimates of food and air Mn exposure is therefore even more compelling. Moreover, because our original intent was to measure only BPb and BAs, Mn-free needles were not used.
Implications. Our study findings led us to ask whether Mn exposure from drinking water might be a concern in the United States. Since 1991, the National Water-Quality Assessment Program (NAWQA NAWQA National Water Quality Assessment Program ) of the U.S. Geological Survey (USGS USGS United States Geological Survey (US Department of the Interior) 2005) has systematically assessed the quality of source water for > 60% of the nation's drinking water. Study areas were selected to represent a variety of important hydrologic and ecologic resources; critical sources of contaminants from agricultural, urban, and natural sources; and a high percentage of population served by municipal water supply and irrigated agriculture. Figure 2 and Table 4 illustrate the distribution of domestic household wells with Mn concentration > 300 [micro]g/L obtained by the USGS NAWQA program. Based on the results of the present report, and the USGS finding that roughly 6% of domestic wells contain more than 300 [micro]g/L, we believe that some U.S. children may be at risk for Mn-induced neurotoxicity.
We thank our Bangladeshi field staff and the people of Araihazar. In particular, we thank M.A.B. Siddique, B. Begum be·gum
1. A Muslim woman of rank.
2. Used as a form of address for such a woman.
[Urdu begam, from East Turkic begüm, first person sing. , and R. Sultana.
Agency for Toxic Substances and Disease Registry. 2001. Toxicological Profile for Manganese. Atlanta, GA:Agency for Toxic Substances and Disease Registry.
Dritish Geological Survey and Department of Public Health and Engineering. 2001. Arsenic contamination of groundwater
tr.v. de·port·ed, de·port·ing, de·ports
1. To expel from a country. See Synonyms at banish.
2. To behave or conduct (oneself) in a given manner; comport. (Kinniburgh DG, Smedley PL, eds). Vol 2. BGS BGS British Geological Survey
BGS Below Ground Surface (depth below the ground surface)
BGS Bundesgrenzschutz (German: Federal Border Guard)
BGS Bachelor of General Studies (degree) Technical Report WC/00/19. Keywerth, UK:British Geological Survey.
Cheng ZY, Zheng Y, Mertlock R, van Geen A. 2004. Rapid multi-element analysis of groundwater by high-resolution inductively coupled plasma mass spectrometry. Anal Bioanal Chem 379:513-518.
Collipp PJ, Chert chert: see flint. SY, Maitinsky S. 1983. Manganese in infant formulas and learning disability. Ann Nutr Metab 27:488494.
Cook D, Fahn S, Brait K. 1974. Chronic manganese intoxication intoxication, condition of body tissue affected by a poisonous substance. Poisonous materials, or toxins, are to be found in heavy metals such as lead and mercury, in drugs, in chemicals such as alcohol and carbon tetrachloride, in gases such as carbon monoxide, and . Arch Neuro130:59-64.
Davis CD, Greger JL. 1992. Longitudinal changes of manganese-dependent superoxide dismutase and other indexes of manganese and iron status in women. Am J Clin Nutr 55:747-752.
Doisy EAJ EAJ Eusko Alderdi Jeltzalea (Basque Nationalist Party)
EAJ Environment Agency of Japan
EAJ Engineering Academy of Japan Inc
EAJ Eat At Joes . 1973. Micronutrient mi·cro·nu·tri·ent
A substance, such as a vitamin or mineral, that is essential in minute amounts for the proper growth and metabolism of a living organism. controls on biosynthesis Biosynthesis
The synthesis of more complex molecules from simpler ones in cells by a series of reactions mediated by enzymes. The overall economy and survival of the cell is governed by the interplay between the energy gained from the breakdown of compounds of clotting proteins and cholesterol. In: Trace Element Substances in Environmental Health (Hemphill DO, ed). Columbia, MO:University of Missouri Press The University of Missouri Press, founded in 1958, is a university press that is part of the University of Missouri System. External link
Finley JW. 1999. Manganese absorption and retention in young women is associated with serum ferritin ferritin /fer·ri·tin/ (-i-tin) the iron-apoferritin complex, one of the chief forms in which iron is stored in the body.
n. concentration. Am J Clin Nutr 70:37-43.
Freeland-Graves J, Lin PH. 1991. Plasma uptake of manganese as affected by oral loads of manganese, calcium, milk, phosphorous phos·pho·rous
Of, relating to, or containing phosphorus, especially with a valence of 3 or a valence lower than that of a comparable phosphoric compound. , copper and zinc. J Am Coil Nutr 10:38-43.
Freeland-Graves J, Tumlund JR. 1996. Deliberations and evaluations of the approaches, endpoints, and paradigms for manganese and molybdenum molybdenum (məlĭb`dənəm) [Gr.,=leadlike], metallic chemical element; symbol Mo; at. no. 42; at. wt. 95.94; m.p. about 2,617°C;; b.p. about 4,612°C;; sp. gr. 10.22 at 20°C;; valence +2, +3, +4, +5, or +6. dietary recommendations. J N utr 128:2435S-2440S.
Gorell JM, Johnson C, Peterson EL. 1999. Occupational metal exposures and the risk of Parkinson's disease Parkinson's disease or Parkinsonism, degenerative brain disorder first described by the English surgeon James Parkinson in 1817. When there is no known cause, the disease usually appears after age 40 and is referred to as Parkinson's disease. . Neureepidemiology 18:303-308.
He, P, Liu, D, Zhang, G, Sunm M. 1994. Effects of high-level manganese sewage irrigation on children's neurebehavior. Chin J Prev Med 28:216-218; cited in Mergler 1999.
Hudnell HK. 1999. Effects from environmental Mn exposures: a review of the evidence from non-occupational exposure studies. NeuroToxicology 20:379-398.
IOM Food and Nutrition Board. 2002. Dietary Reference Intakes dietary reference intakes (DRIs),
n.pl a set of nutritional guidelines concerning the intake of vitamins and minerals from food rather than supplements. : Vitamin A vitamin A
also called retinol
Fat-soluble alcohol, most abundant in fatty fish and especially in fish-liver oils. It is not found in plants, but many vegetables and fruits contain beta-carotene (see , Vitamin K, Arsenic, Boron boron (bōr`ŏn) [New Gr. from borax], chemical element; symbol B; at. no. 5; at. wt. 10.81; m.p. about 2,300°C;; sublimation point about 2,550°C;; sp. gr. 2.3 at 25°C;; valence +3. , Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium vanadium (vənā`dēəm), metallic chemical element; symbol V; at. no. 23; at. wt. 50.9415; m.p. about 1,890°C;; b.p. 3,380°C;; sp. gr. about 6 at 20°C;; valence +2, +3, +4, or +5. Vanadium is a soft, ductile, silver-grey metal. and Zinc. Washington, DC:National Academy Press.
IOM Food and Nutrition Board. 2004. Dietary Reference Intakes for Water, Potassium, Sodium, Chloride, and Sulfate sulfate, chemical compound containing the sulfate (SO4) radical. Sulfates are salts or esters of sulfuric acid, H2SO4, formed by replacing one or both of the hydrogens with a metal (e.g., sodium) or a radical (e.g., ammonium or ethyl). . Washington, DC:National Academy Press.
Kaiser J. 2003. Manganese: A high-octane dispute. Science 300:926-928.
Kondakis KG, Leotsinidis M, Papapetropoulos T. 1985. Possible health effects of high manganese concentration in drinking water. Arch Environ Health 44:176-178.
McCarthy D. 1972. Manual for the McCarthy Scales of Children's Abilities. San Antonio, IX:Psychological Corporation.
Mena I, Matin S, Fuenzalida S. 1967. Chronic manganese poisoning manganese poisoning Acute or chronic intoxication due to manganese excess Etiology Industrial exposure to manganese-laden fumes and dusts in mining, steel foundries, welding, battery manufacture Clinical Acute–pneumonitis; chronic–psychotic or : clinical picture and manganese turnover. Neurology 17:126-136.
Mergler D. 1999. Neurotoxic effects of low level exposure to manganese in human populations. Environ Res 80:99-102.
Mergler D, Baldwin M. 1997. Early manifestations of manganese neurotoxicity in humans: an update. Environ Res 73:92-100.
National Research Council 1973. Manganese. Washington, DC:National Academy of Sciences.
Nixon DE, Mussmann GV, Ecktahdahl S J, Meyer TP. 1991. Total arsenic in urine: palladium-persulfate vs nickel as a matrix modifier (programming) modifier - An operation that alters the state of an object. Modifiers often have names that begin with "set" and corresponding selector functions whose names begin with "get". for graphite furnace atomic absorption Graphite furnace atomic absorption spectrometry (GFAAS) (also known as Electrothermal Atomic Absorption Spectrometry (ETAAS)) is a type of spectrometry that uses a graphite-coated furnace to vaporize the sample. spectrophotometry spectrophotometry
Branch of spectroscopy dealing with measurement of radiant energy transmitted or reflected by a body as a function of wavelength. The measurement is usually compared to that transmitted or reflected by a system that serves as a standard. . Clin Chem 37:1575-1579.
Pruszkowski E, Neubauer K, Thomas R. 1998. An overview of clinical applications by inductively coupled plasma mass spectrometry. Atom Spectrosc 19:111-115.
Raven JC, Court JH, Raven J. 1983. Manual for Raven's Progressive Matrices Raven's Progressive Matrices (often referred to simply as Raven's Matrices) are multiple choice tests of abstract reasoning, originally developed by Dr John C. Raven in 1938. and Vocabulary Scales (Section 3)--Standard Progressive Matrices. London:Lewis.
Reels HA, Ortega-Eslava MI, Ceulemans E, Robert A, Lison D. 1999. Prospective study on the reversibility of neurobehavioral effects in workers exposed to manganese dioxide. NeuroToxicolegy 20:255-271.
Stroh A. 1988. Determination of Pb and Cd in whole blood using isotope dilution ICP-MS. Atom Spectrosc 14:141-143.
Takeda A. 2003. Manganese action in brain function. Brain Res Rev 41:79-87.
Takser L, Mergler D, Hellier G, Sahuqillo J, Huel G. 2003. Manganese, monoamine monoamine /mono·amine/ (mon?o-ah-men´) an amine containing one amino group, e.g., serotonin, dopamine, epinephrine, and norepinephrine.
n. matabolite levels at birth, and child psychomotor development. NeuroToxicology 24:667-674.
U.S. EPA. 1999. A Review of Contaminant contaminant /con·tam·i·nant/ (kon-tam´in-int) something that causes contamination.
something that causes contamination. Occurrence in Public Water Systems. EPA Report 816- R-99/006. Washington, DC:U.S. Environmental Protection Agency, Office of Water.
U.S. EPA. 2004. Drinking Water Health Advisory for Manganese. Report 822D04003. Washington, DC:U.S. Environmental Protection Agency. Available: http://www.apa.gov/safewater/ccl/pdf/dwadvisory manganese health [accessed 1 October 2005].
USGS. 2005. National Water-Quality Assessment Program. Reston, VA:U.S. Geological Survey. Available: http://water.usgs.gov/nawqa/[accessed 1 October 2005].
van Geen A, Cheng Z, Seddique AA, Hoque A, Gelman A, Graziano JH, eta[. 2005. Reliability of a commercial kitto test groundwater for arsenic in Bangladesh. Environ Sci Technol 39:299-303.
van Geen A, Zheng Y, Versteeg R, Stute M, Horneman A, Dhar R, et al. 2003. Spatial variability of arsenic in 6000 tube wells in a 25 k[m.sup.2] area of Bangladesh. Water Desources Res 39:1140-1150.
Wasserman GA, Liu X, Parvez F, Ahsan H, Factor-Litvak P, van Geen A, et al. 2004. Water arsenic exposure and children's intellectual function in Araihazar, Bangladesh. Environ Health Perspect 112:1329-1333.
Wechsler D. 1991. Manual for the WISC-III. San Antonio, Ix:Psychological Corporation.
WHO. 2002. Principles and Methods for the Assessment of Risk from Essential Trace Elements Trace elements
A group of elements that are present in the human body in very small amounts but are nonetheless important to good health. They include chromium, copper, cobalt, iodine, iron, selenium, and zinc. Trace elements are also called micronutrients. . Environmental Health Criteria 228. Geneva Geneva, canton and city, Switzerland
Geneva (jənē`və), Fr. Genève, canton (1990 pop. 373,019), 109 sq mi (282 sq km), SW Switzerland, surrounding the southwest tip of the Lake of Geneva. :World Health Organization.
This work was supported by National Institute of Environmental Health Sciences The National Institute of Environmental Health Sciences (NIEHS) is one of 27 Institutes and Centers of the National Institutes of Health (NIH),which is a component of the Department of Health and Human Services (DHHS). The Director of the NIEHS is Dr. David A. Schwartz. grants P42 ES 10349 and P30 ES 09089, the Mailman School of Public Health, the Lamont-Doherty Earth Observatory, and the Earth Institute at Columbia University.
The authors declare they have no competing financial interests.
Gaff A. Wasserman, (1,2) Xinhua Liu, (2,3) Faruque Parvez, (4) Habibul Ahsan, (5) Diane Levy, (3) Pam Factor-Litvak, (5) Jennie Kline, (2,5,6) Alexander van Geen, (7) Vesna Slavkovich, (4) Nancy J. Lolacono, (4) Zhongqi Cheng, (7) Yan Zheng, (7,8) and Joseph H. Graziano (4)
(1) Department of Psychiatry, College of Physicians and Surgeons College of Physicians and Surgeons: see Columbia Univ. , Columbia University, New York, New York, USA; (2) New York State Psychiatric Institute The New York State Psychiatric Institute, established in 1895, was one of the first institutions in the United States to integrate teaching, research and therapeutic approaches to the care of patients with mental illnesses. , New York, New York, USA; (3) Department of Biostatistics, (4) Department of Environmental Health Sciences, and (5) Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, New York, USA; (6) Gertrude H. Sergievsky Center, Columbia University, New York, New York, USA; (7) Lamont-Doherty Earth Observatory, Columbia University, Palisades, New York Palisades, New York is a very small hamlet, part of the Town of Orangetown, located in southeastern Rockland County, New York. It borders the Hudson River to the east, Rockleigh and Alpine New Jersey to the south, Tappan to the west, and Sparkill to the north. , USA; (8) Queens College, City University of New York Queens College is one of the senior colleges of the City University of New York.
History and enrollment
Queens College was established in 1937 to serve the needs of the growing borough's population, including newly arrived immigrant families. , New York, New York, USA
Address correspondence to G.A. Wasserman, New York State Psychiatric Institute, 1051 Riverside Dr., Unit 78, New York, NY 10032 USA. Telephone: (212) 543-5296. Fax: (212) 543-1000. E-mail: firstname.lastname@example.org
Received 17 February 2005; accepted 9 August 2005.
Table 1. Characteristics of study participants. (a) Variable Mean [+ or -] SD Male (b) 70 (49.3) TV access (b) 52 (36.6) House type (b) Thatched roof or poorer 20 (14.1) Corrugated tin 101 (71.1) Concrete construction 21 (14.8) Father's occupation (b) Other/missing 19 (13.4) Laborer/farmer 24 (16.9) Factory/other paid job 49 (34.5) Business 50 (35.2) Child age 10.0 [+ or -] 0.4 Full-Scale IQ 64.5 [+ or -] 11.6 Verbal IQ 70.8 [+ or -] 12.2 Performance IQ 63.9 [+ or -] 11.9 Full-Scale raw score 71.2 [+ or -] 22.9 Verbal raw score 15.9 [+ or -] 5.4 Performance raw score 55.4 [+ or -] 18.9 Height (cm) 126.5 [+ or -] 6.7 Weight (kg) 22.4 [+ or -] 3.7 Body mass index 13.9 [+ or -] 1.3 Head circumference (cm) 49.5 [+ or -] 1.5 Mother's education (years) 3.1 [+ or -] 3.5 Father's education (years) 3.9 [+ or -] 3.8 Mother's age (years) 33.8 [+ or -] 6.3 Mother's Raven score 14.1 [+ or -] 3.1 WMn ([micro]g/L) 795 [+ or -] 755 WAs ([micro]g/L) 3.0 [+ or -] 2.6 UAs ([micro]g/L) 57.5 [+ or -] 67.6 UCr ([micro]g/dL) 45.4 [+ or -] 30.2 UAs ([micro]g/g creatinine) 133.0 [+ or -] 86.8 Hgb (c) ([micro]/dL) 12.6 [+ or -] 1.1 BPb (c) ([micro]g/dL) 12.0 [+ or -] 3.7 BMn (c) ([micro]g/L) 12.8 [+ or -] 3.2 BAs (c) ([micro]g/L) 4.3 [+ or -] 1.9 (a) Except where noted, sample size is 142. (b) Values reflect n (%) 1. (c) n=95. Table 2. Unadjusted associations (Spearman correlation coefficients) among measures of exposure to As, Pb, and Mn. UAs ([micro]g/g WMn BAs creatinine) ([micro]g/L) ([micro]g/L) WAs 0.27 ** 0.36 # 0.30 ** UAs ([micro]g/ 0.16 0.51 * g creatinine) WMn 0.23 * BAs 0.02 -0.11 BMn 0.13 BMn BPb ([micro]g/L) ([micro]g/dL) WAs -0.05 -0.06 UAs ([micro]g/ -0.03 -0.06 g creatinine) WMn -0.04 -0.13 BAs 0.02 -0.11 BMn 0.13 For comparisons between water and urinary concentrations, n= 142. Correlations involving whole blood metal concentrations are for the subset of 95 children who gave blood samples. * p<0.05, ** p<0.01, # p<0.001. Table 3. Predicting Verbal, Performance, and Full-Scale raw scores from WMn before and after covariate adjustment: unstandardized regression B-coefficient. Variable Full-Scale Performance Verbal Before adjustment WMn ([micro]g/L) -5.20 # -4.43 -0.80 * After adjustment Maternal education (years) None -6.09 -2.95 -2.72 * 1-5 -1.26 -0.37 -0.59 5-13 (a) -- - - Maternal intelligence 0.43 0.38 0.07 House type Thatched roof or poorer -5.73 -6.02 0.21 Corrugated tin -0.60 0.63 0.06 Concrete (a) TV access 2.32 1.98 0.48 Height (cm) 0.79 ** 0.62 ** 0.16 * Head circumference (cm) 3.48 ** 3.01 ** 0.50 WMn ([micro]g/L) -4.35 # -3.76 # -0.63 * Total [R.sup.2] (%) 31.29 31.01 20.11 [R.sup.2], total variance explained. (a) Reference group. * p < 0.05, ** p < 0.01, # p < 0.001. Table 4. Mn in U.S. domestic groundwater wells (n = 2,624). Cumulative Mn ([micro]g/dL) Frequency Percent percent < 200 2,386 90.9 90.9 201-300 81 3.1 94.0 301-500 71 2.7 96.7 501-1,000 56 2.1 98.9 > 1000 30 1.1 100.0