Vivascience AG Offers Complete Kit for Adenovirus Purification.

HANOVER, Germany -- Vivapure(R) AdenoPACK(TM) 100 for Unprecedented Fast and Efficient Adenovirus Purification

Vivascience AG, a subsidiary of Sartorius AG, introduces Vivapure AdenoPACK 100, a ready-to-use kit for adenovirus purification, to the market.

The new kit includes all reagents and devices necessary for clarification, purification and concentration of adenovirus from HEK293 cells. The rapid two-hour purification protocol is based on Vivascience's ion exchange membranes incorporated in AdenoPACK syringe filter units. The device is unique in its capability to efficiently capture and remove large virus particles. When compared to chromatography media, membrane adsorbers provide large 3000 nm pores allowing unrestricted access and recovery of adenovirus from the charged adsorber surface. In a fast and simple procedure, recoveries of up to 1-5 x 10 E12 purified virus particles per millilitre can be obtained for in-vitro transfections.

In contrast to traditional Caesium Chloride density gradient methods, virus purification with Vivapure AdenoPACK is fast, simple, not toxic to virus or cells and leads to a higher virus titre using the same amount of original cell culture. Additionally, expensive equipment such as an ultracentrifuge is no longer needed.

The membrane adsorbers with porous matrices, high capacities, low differential pressure, high flow rates, and low unspecific adsorption show an excellent performance in small-scale virus purification. However, they are also scalable to confirm to cGMP facilities to large volume, high performance separation, reducing the processing time by a factor of 10 in the final process.

For further information on Vivascience protein purification products, on Vivascience ultrafiltration and filtration products, please visit our website at www.vivascience.com.

Vivascience/Sartorius

Vivascience AG develops innovative solutions for protein purification and analysis with a product portfolio of device formats for filtration, ultrafiltration, and cell culture. Vivascience is a 100 percent owned subsidiary of Sartorius AG, Goettingen, an internationally leading laboratory and process technology supplier. In 2003, the technology group earned sales revenue of 442.3 million euros and employed a good 3,660 persons. Its biotechnology segment focuses on filtration and separation products, bioreactors and proteomics. The mechatronics segment manufactures weighing equipment and systems for laboratory and industrial applications.