University of Utah obtains United States patent.University of Utah The University of Utah (also The U or the U of U or the UU), located in Salt Lake City, is the flagship public research university in the state of Utah, and one of 10 institutions that make up the Utah System of Higher Education. (Salt Lake City, UT) has patented a method for making a directed antisense antisense, DNA or RNA manipulated in a laboratory so that its components (nucleotides) form a complementary copy of normal, or "sense," messenger RNA (mRNA; see nucleic acid). library against a target transcript is described. A cDNA of the target transcript is cloned in an appropriate cloning vector cloning vector n. An autonomously replicating plasmid having regions into which foreign DNA can be inserted. . Next, a plurality of deletion derivatives of the cloned cDNA is prepared such that the deletions serially extend into the cDNA from one end thereof. The resulting deletion library is then treated such that cDNA is removed from the other end of each cDNA insert, thus obtaining a fragment library having fragments of a selected size. A catalytic core is then inserted into each fragment of the fragment library, resulting in the directed antisense library. An illustrative antisense gene in the hammerhead ribozyme Hammerhead RNAs are small self-cleaving RNAs that have a conserved motif found in several of the viroids and satellite RNAs associated with plant RNA viruses and other species, and that replicate via a rolling circle mechanism. catalytic core. Plasmids for making the antisense library, plasmids and methods for making the fragment library, and a method for identifying target sites for antisense-mediated gene inhibition are also described. (US 6,586,180) |
|
||||||||||||||||||||
Printer friendly
Cite/link
Email
Feedback
Reader Opinion