The function of trichomes of an amphibious fern, Marsilea quadrifolia.
ABSTRACT.--Marsilea quadrifolia, an amphibious fern, has the ability to develop heterophyllous, aerial and submerged leaves. In contrast to submerged leaves, aerial leaves have trichomes on both surfaces. To examine if the presence of trichomes can reflect excess light and hence reduce the risk of being damaged by excess light, we compared the optical properties and chlorophyll a fluorescence, in particular the maximum PSII photochemical efficiency (Fv/Fm), of M. quadrifolia leaves with trichomes (intact) and those having trichomes removed (de-trichomed). Photosynthetic gas exchange measurements were also conducted to quantify transpirational water loss and instantaneous water use efficiency (WIDE) of M. quadrifolia intact and de-trichomed leaves. The results showed that removal of trichomes neither affected the optical properties in the visible part of the solar spectrum nor the midday depression of Fv/Fm values of leaflets. In contrast, significantly increased in transpiration rates and decreased rates in WUE were found in detrichomed leaflets in comparison to intact ones. These results imply that the presence of trichomes is of more importance in reducing water loss than in reflecting light and protecting M. quadrifolia against the potentially damaging effect of photoinhibition in aerial environments.
KEY WORDS.--amphibious fern, gas exchange, Marsilea quadrifolia, optical property, photoinhibition, trichome
How amphibious species cope with contrasting environmental conditions between aquatic and terrestrial habitats is of interests to researchers. Marsilea, an amphibious fern genus, has the ability to develop heterophyll. Marsilea quadrifolia L. experiencing extreme variation in environment develops submerged, floating, emergent and terrestrial leaves (Liu, 1984; Lin et al., 2007). These different forms of leaves have different morphological and physiological characteristics. For example, in contrast to the glabrous surface of submerged and floating leaves, the adaxial and abaxial surfaces of emergent leaves are covered with dense trichomes. In addition, leaves of terrestrial grown M. quadrifolia (terrestrial leaves) have more trichomes than emergent leaves of aquatic grown (Lin et al., 2007). The ecological importance of these trichomes has not been studied.
The two commonly cited functions of trichomes are to increase reflectance and to increase boundary layer resistance (Lambers et al., 1998). Increasing reflectance would reduce incident light on leaf surfaces and might reduce the risk of overheating and the potentially harmful effects of excessive light on leaves (Ehleringer, 1984). Increasing boundary layer resistance would reduce transpirational water loss. A combination of drought, temperature and light stress would greatly increase in terrestrial environments in comparison to that in aquatic habitats. Hence, the production of trichomes may represent one of the acclimation responses conferring M. quadrifolia the ability to grow in terrestrial conditions (Lin et al., 2007).
The phenomenon of photoinhibition occurs when leaves are exposed to light levels in excess of what can be utilized in photosynthesis (Powles, 1984). Photoinhibition leads to decreases in photon yield and photosystem II is considered the primary site of photoinhibition (Barber and Andersson, 1992). The yield and kinetics of chlorophyll a fluorescence emitted from leaves upon illumination with actinic light have been used as a probe for the primary photochemistry of photosynthesis (Krause and Weis, 1991). In particular, the linear relationship between quantum yield and the ratio of variable fluorescence to maximum fluorescence (Fv/Fm) (Kao and Forseth, 1992), suggests that Fv/Fm can be used as a probe to monitor the activity of photosynthetic carbon assimilation. A decrease in the values indicates reduction in photosynthetic activity.
The objective of this study is to investigate the ecological significance of trichomes in leaves of M. quadrifolia. To examine if the presence of trichomes can reflect excess light and hence reduce the risk of being damaged by excess light, we compared the optical properties and chlorophyll a fluorescence of M. quadrifolia intact leaves (with trichomes) with leaves having trichomes removed (by de-trichomed treatment). Photosynthetic gas exchange measurements were also conducted to quantify transpirational water loss and instantaneous water use efficiency (WUE) of M. quadrifolia intact and detrichomed leaves. We test the hypothesis that to reduce the risk of drought stress and being damaged by excessive light, M. quadrifolia develops trichomes, reducing transpirational water loss and/or reflecting excess light.
MATERIAL Am METHODS
Rhizomes of M. quadrifolia were planted in 2 L plastic pots filled with a mixture of vermiculite: soil of 1:1 by volume. Plants were grown in a glasshouse receiving natural daylight, watered to soil saturation every other day, and fertilized using inorganic fertilizer (N:P:K of 20:20:20) once every two weeks. The plant produces leaves with four leaflets expanded on a plane perpendicular to the petiole, resembling a four-leaf clover, which is connected to the rhizome. The following measurements were conducted on leaflets.
The morphology of trichomes was observed and the length measured under a scanning electron microscope (TM 1000, Hitachi). The optical properties were measured on the same leaflet before and after partial trichomes being removed. To remove trichomes, we gently brushed both surfaces of leaflets. Trichome density was estimated on both surfaces with a dissecting microscope before and after the de-trichomed treatment. Leaf optical measurements on adaxial surfaces were made using a custom-built dual integrating sphere system following the method described by Runcie and Durako (2004). Briefly, leaf spectral transmittance (T([lambda])) and reflectance (R([lambda])) were measured from 400 nm to 700 nm at 0.5 nm resolution using a fiber-optic spectrometer (HR2000, Ocean Optics) interfaced with a FOI[s.sup.-1] (for T([lambda]) measurement; Ocean Optics) or ISP-REF (for R([lambda]) measurement; Ocean Optics) integrating spheres. Light source provided by a collimated beam from a tungsten-halogen light (L[s.sup.-1], Ocean Optics) was directed into the entrance port and to an exit port of the opposite side of the sphere through an optical fiber. For measuring reflectance, measurements were calibrated against a 99% reflectance standard (W[s.sup.-1]-SS, Ocean Optics). After measurements of T([lambda]) and R([lambda]), we then calculated leaf absorptance (A([lambda]) = 1 - T([lambda]) - R([lambda])).
To evaluate if the presence of trichomes can reduce photoinhibition, we measured the characteristics of fluorescence induction on intact and treated leaflets in situ using a portable, pulse amplitude modulated fluorometer (MiniPAM, Walz, Effeltrich, Germany). Fluorescence was measured on the adaxial side of leaflets with or without trichomes removed (n = 6) at 10, 12 and 14 h on a clear day. Leaves were adapted to darkness for 30 minutes before the measurement was taken. Photosynthetic photon flux (PPF) on a horizontal surface at the same height of the leaves and air temperature were also monitored.
Photosaturated photosynthetic rates ([A.sub.max]) and transpiration rates (E) were measured with an LI-6400 infrared gas exchange system (LI-Cor, Lincoln, Nebraska, USA) on the most recently expanded, intact and de-trichomed leaflets. The intercellular C[O.sub.2] concentration (Ci) was calculated according to Farquhar and Sharkey (1982). Measurement conditions within the cuvette were: photosynthetic photon flux density (PPF) of 1200 [micro]mol [m.sup.-2][s.sup.-1], cuvette temperature 30[degrees]C, leaf-to-air water vapor pressure difference (VPD) 1.5-1.6 kPa, and ambient C[O.sub.2] concentration 360 [+ or -] 5 [cm.sup.3][m.sup.-3]. The de-trichomed leaflets remained green and looked healthy a few days after the experiment indicating that the brushing treatment did not damage the leaflets. To further make sure that the de-trichomed treatment did not damage the epidermis, we also made paraffin sections of leaflets and found that the epidermis cells remained intact after the detachment of trichomes (data not shown).
All statistical tests were performed with the computer software SYSTAT (Statistical Solution, Cork, Ireland). Significant differences are reported as P < 0.05.
Characteristics of trichomes.--The morphology of trichomes is shown in the SEM picture (Fig. 1). Grown in terrestrial condition, M. quadrifolia produced multicellular, 2-3 cells, trichomes (Fig. 1). Before making the SEM scan, we used liquid nitrogen to fix the samples. Some of trichomes were detached from the surface by the treatment. Hence, we estimated trichome density with a dissecting microscope instead of calculating the number of trichomes by SEM scanning. The result showed that there was no significant difference in trichome density between the adaxial and abaxial surfaces (Table 1).
[FIGURE 1 OMITTED]
Leaf optical properties.--In general, intact and de-trichomed leaflets had the highest reflectance and transmittance and the lowest absorptance at ca. 550 nm in the wavelengths ranging from 400 to 700 nm (Fig. 2). In a comparison of intact and de-trichomed leaves, no significant difference was found either in T([lambda]) or in R([lambda]). Consequently, both types of leaves had similar A([lambda]). As a result, reducing trichome density did not affect the average reflectance, transmittance and absorptance of visible light in M. quadrifolia leaflets (Table 1).
[FIGURE 2 OMITTED]
Chlorophyll fluorescence measurement.--The diurnal courses of PPF at plant height and air temperature (Ta) were recorded on the same day as leaf fluorescence was measured (Fig. 3a). Initial and continuous dark adapted measurements of maximum PSII photochemical efficiency (Fv/Fm) indicated that the leaves were healthy and not experiencing stress due to the removal of trichomes (Table 2). Midday depression of Fv/Fm values was found in the leaflets when exposed to solar irradiation (Fig. 3b). In comparison to continuously dark-adapted leaves, illuminated leaflets showed significant reduction in Fv/Fm at 1000h, 1200h and 1400h when PPF and air temperature were highest during the day (Fig. 3). No significant difference was found in Fv/ Fm values between intact and de-trichomed leaflets.
Gas exchange measurement.--Transpiration rate (E) increases with [A.sub.max] in both intact and detrichomed leaflets; as a result, a significant, positive, linear relationship was found between [A.sub.max] and E (Fig. 4a). No significant difference was found between the slopes of these positive relationships for intact and de trichomed leaflets. However, de-trichomed leaflets had significantly higher E relative to intact leaflets for a given [A.sub.max].
The calculated Ci values of de-trichomed leaflets were higher than those of intact leaflets (Fig. 4h).
[FIGURE 3 OMITTED]
Knowledge of how fern species cope with excess light or drought stress in terrestrial environments is of ecological and evolutionary importance. Biochemical mechanisms, such as xanthophyll-mediated energy dissipation, and/or morphological mechanisms, such as leaf curing and laminar scales, have been demonstrated in pteridophytes (Eichmeier et al., 1993; Tausz et al., 2001; Watkins et al., 2006). To our knowledge, the influence of pubescence on the incident radiation and water budget has not been studied in any fern species.
Among other functions (Johnson, 1975; Zvereva et al., 1998), pubescence had been shown to increase reflectance (Ehleringer, 1984; Holmes and Keiller, 2002) and afford protection against excess radiation (Ripley et al., 1999; Morales et al., 2002; Manetas, 2003) in seed plants. Our results, however, showed that M. quadrifolia leaflets have a very high absorptance and detrichomed treatments did not affect the visible part (400-700 nm) of optical properties of the leaflets (Fig. 2). Additionally, the Fv/Fm values of leaflets of M. quadrifolia at the midday were not affected by the removal of trichomes (Fig. 3b). It is therefore possible that trichomes on leaflets of M. quadrifolia are of less importance in reflecting light and in protecting the plant against the potentially damaging effect of photoinhibition. However, leaf temperatures in de-trichomed leaflets may be reduced due to their increased transpiration rates (Fig. 4a), which may ameliorate the damaging effect of high light and high air temperature on de-trichomed leaflets. For example, the result that the detrichomed/orienting leaflets had less reduction in Fv/Fm, though not significant, than intact/orienting leaflets (Fig. 3b) might result from the increased transpiration rate in the former. Accordingly, we cannot completely exclude the role of trichomes in providing photoprotection for M. quadrifolia leaflets.
Few studies have also shown that the presence of trichomes can reduce leaf transpiration rates (Ripley et al., 1999). The significantly increase in transpiration rates, about 30%, measured in de-trichomed M. quadrifolia leaflets compared to intact ones (Fig. 4a) suggests that the presence of trichomes is of more importance in reducing water loss than in providing photoprotection. At constant leaf-air vapor pressure deficit, Ci values may be used as a measure of the instantaneous water use efficiency (WUE) of the leaf (Farquhar and Sharkey, 1982), with a lower Ci indicating a higher instantaneous WUE. De-trichomed treatments resulted in leaflets with higher Ci (Fig. 4b) implying a lower WUE. These results reveal that the reduction in water loss from M. quadrifolia leaflets with trichomes also resulted in increased WUE.
[FIGURE 4 OMITTED]
The hair layer on leaves may lead to higher leaf temperatures caused by reducing the transpirational water loss (Ripley et al., 1999). We have observed that leaflets of M. quadrifolia have the ability of performing tropic movements (pers. obs.). It is possible that M. quadrifolia adjust leaflet angle and azimuth to intercept a smaller quantity of radiant energy, which would allow the plant to moderate leaflet temperature without excessive transpiration. The function of tropic leaf movements in protecting soybean leaves from photoinhibition has been documented (Kao and Forseth, 1992). Thus, the presence of trichomes on both surfaces combined with leaflet movements may provide M. quadrifolia mechanisms against drought stress. Accordingly, we hypothesize that the combination of the avoidance mechanisms, leaf movements and the production of trichomes are very important adaptations, conferring the amphibious M. quadrifolia ability to grow in terrestrial conditions. The effect of the interaction between water availability and light intensity on trichome density and leaflet movements in M. quadrifolia are currently under studied.
We thank Dr. Bai-Ling Lin, for the inspiration of this study and providing rhizomes of M. quadrifolia, Dr. Shiang-Jiuun Chen for providing technique support in taking the SEM picture, and Yih-Chi Chang, for help in taking chlorophyll fluorescence and gas exchange measurements.
BARBER, J. and B. ANDERSSON. 1992. TOO much of a good thing: light can be bad for photosynthesis. Trends in Biochem. Sci. 17:61-66.
EHLERINGER, J. R. 1984. Ecology and ecophysiology of leaf pubescence in North American desert plants. In E. Rodrigues, P. L. Healey and I. Mehta, eds. Biology and Chemistry of Plant Trichomes. Plenum Press, New York.
EICHMEIER, W. C., C. CASPER and C. B. OSMOND. 1993. Chlorophyll fluorescence in the resurrection plant Selaginella lepidophylla (Hook & Grev) Spring during high-light and desiccation stress, and evidence for zeaxanthin-associated photoprotection. Planta 189:30-38.
FARQUHAR, G. D. and T. D. SHARKEY. 1982. Stomatal conductance and photosynthesis. Ann. Rev. Plant Physiol. 33:317-345.
JOHNSON, H. B. 1975. Plant pubescence- ecological perspective. Bot. Rev. 41:233-258.
HOLMES, M. G. and D. R. KEILLER. 2002. Effects of pubescence and waxes on the reflectance of leaves in the ultraviolet and photosynthetic wavebands: a comparison of a range of species. Plant Cell Environ. 25:85-93.
KAO, W.-Y. and I. N. FORSETH. 1992. Diurnal leaf movement, chlorophyll fluorescence and carbon assimilation in soybean grown under different nitrogen and water availability. Plant Cell Environ. 15:703-710.
KRAUSE, G. H. and E. WEIS. 1991. Chlorophyll fluorescence and photosynthesis: the basics. Ann. Rev. Plant Physiol. Plant Mol. Biol. 42:313-349.
LAMBERS, H., F. S. CHAPIN III and T. L. PONS. 1998. Plant Physiological Ecology. Springer, New York.
LIN, C.-H., B.-L. LIN and W.-Y. KAO. 2007. Leaf characteristics and photosynthetic performance of floating, emergent and terrestrial leaves of Marsilea quadrifolia. Taiwania 52:195-200.
LIU, B. L. L. 1984. Abscisic acid induces land form characteristics in Marsilea quadrifolia L. Am. J. Bot. 71:638-644.
MANETAS, Y. 2003. The importance of being hairy: the adverse effects of hair removal on stem photosynthesis of Verbascum speciosum are due to solar UV-B radiation. New Phytol. 158:503-508.
MORALES, F., A. ABRADIA, J. ABADIA, G. MONTSERRAT and E. GRL-PELEGRIN. 2002. Trichomes and photosynthetic pigment composition changes: responses of Quercus ilex subsp, blllota (Desf.) Samp. and Quercus coccifera L. to Mediterranean stress conditions. Trees 16:504-510.
POWLEs, S. B. 1984. Photoinhibition of photosynthesis induced by visible light. Ann. Rev. Plant Physiol. 35:15-44.
RIPLEY, B. S., N. W. PAMMENTER and V. R. SMITH. 1999. Function of leaf hairs revisited: the hair layer on leaves of Arctotbeca populifolia reduces photoinhibition, but leads to higher leaf temperatures caused by lower transpiration rates. J. Plant Physiol. 155:78-85.
RUNCIE, J. W. and M. J. DURAKO. 2004. Among-shoot variability and leaf-specific absorptance characteristics affect diel estimates of in situ electron transport of Posidonia australis. Aquat. Bot. 80:200-209.
TAUSZ, M., P. HIETZ and O. BRIONES. 2001. The significance of carotenoids and tocopherols in photoprotection of seven epiphytic fern species of a Mexican cloud forest. Aust. J. Plant Physiol. 28:775-783.
WATKINS, J. E., A. Y. KAWAHARA, S. T. LEICHT, J. R. AULD, A. J. BICKSLER and K. KAISER. 2006. Fern laminar scales protect against photoinhibition from excess light. Am. Fern J. 96:83-92.
ZVEREVA, E. L., M. V. KOZLOV and P. NIEMELA. 1998. Effects of leaf pubescence in Salix borealis on host-plant choice and feeding behavior of the leaf beetle, Melasoma lapponica. Ento. Exp. Appl. 89:297-303.
Inst. of Ecology and Evolutionary Biology, National Taiwan University
WEN-YUAN KAO *
Inst. of Ecology and Evolutionary Biology, National Taiwan University,
Department of Life Science, National Taiwan University
* Corresponding author: Wen-Yuan Kao; Address: Department of Life Science, National Taiwan University, Taipei, Taiwan; Fax: 886-2367-3374, Phone: 886-3366-2511; e-mail: firstname.lastname@example.org
TABLE 1. Trichome density ([cm.sup.-2]) on adaxial and abaxial surfaces of intact and de-trichomed leaflets and the optical properties of M. quadrifolia (mean [+ or -] S.E., n = 5). Values within the same row followed by different superscripts represent significant difference at P = 0.05. Parameters Intact leaflets Trichome density Adaxial surface 1433 [+ or -] 69 (a) Abaxial surface 1594 [+ or -] 99 (a) Total 3027 [+ or -] 164 (a) Optical properties Reflectance (%) 5.5 [+ or -] 0.4 (a) Transmittance (%) 6.5 [+ or -] 0.9 (a) Absorptance (%) 88.0 [+ or -] 1.0 (b) Parameters De-trichomed Trichome density 149 [+ or -] 47 (b) 159 [+ or -] 39 (b) 308 [+ or -] 77 (b) Optical properties 5.3 [+ or -] 1.0 (a) 7.8 [+ or -] 1.1 (a) 87.0 [+ or -] 1.7 (b) TABLE 2. Maximum PSII photochemical efficiency (Fv/Fm) of initial (before leaflet being exposed to solar irradiation) and continuous dark adapted intact and de-trichomed leaflets of M. quadrifolia used in the measurement of chlorophyll fluorescence (mean [+ or -] s.e., n = 6). Treatments Initial Continuous dark Intact 0.76 [+ or -] 0.01 0.77 [+ or -] 0.01 De-trichomed 0.77 [+ or -] 0.01 0.77 [+ or -] 0.01