TRADE NEWS: Agilent Technologies Introduces Innovative Technology to Separate Proteins and Peptides; Unique Fractionation System Allows Scientists to Identify More Proteins, Peptides and Biomarkers Using LC/MS.SEATTLE -- Agilent Technologies Inc. (NYSE NYSE See: New York Stock Exchange :A) today introduced an innovative technology that fractionates proteins and peptides with unprecedented simplicity and resolution. Co-developed with DiagnoSwiss S.A., the Agilent 3100 OFFGEL Fractionator enables scientists to identify more proteins, peptides and potential biomarkers by liquid chromatography/mass spectrometry (LC/MS LC/MS Liquid Chromatography/Mass Spectrometry ). Biological samples such as blood plasma blood plasma n. The yellow or gray-yellow, protein-containing fluid portion of blood in which the blood cells and platelets are normally suspended. and cells typically contain tens of thousands of proteins over a wide range of concentrations. This high complexity makes it difficult to identify low-abundance proteins that play important roles in normal biological processes and disease. The OFFGEL Fractionator improves researchers' ability to identify low-abundance proteins and peptides by reducing sample complexity and greatly increasing the sensitivity of LC/MS analysis. "The OFFGEL system for protein and peptide fractionation fractionation /frac·tion·a·tion/ (frak?shun-a´shun) 1. in radiology, division of the total dose of radiation into small doses administered at intervals. 2. has helped us increase our dynamic range and dig deeper into the proteome pro·te·ome n. The complete set of proteins that are produced by the genes of an organism. proteome the entire complement of proteins produced by a cell. ," said Konstantinos Petritis, senior scientist at Pacific Northwest National Laboratory The Pacific Northwest National Laboratory (PNNL) is one of nine United States Department of Energy (DOE) multiprogram national laboratories. The laboratory PNNL is located in Richland, Washington, and operates a marine research facility in Sequim, Washington. . "The OFFGEL Fractionator further demonstrates Agilent's commitment to and leadership in advancing the field of proteomics," said Taia Ergueta, general manager of Agilent's mass spectrometry mass spectrometry or mass spectroscopy Analytic technique by which chemical substances are identified by sorting gaseous ions by mass using electric and magnetic fields. business. "We designed the technology to be a valuable and affordable addition to any lab doing proteomics research. Used in concert with our industry-leading separation technologies, LC/MS systems, software and bioinformatics platforms, the OFFGEL system will increase productivity and facilitate the discovery process." The latest addition to Agilent's protein separation solutions for proteomics research, the OFFGEL Fractionator provides a novel means of fractionating both intact proteins and peptides by their isoelectric point isoelectric point n. The pH at which the electrolyte concentration of an amphoteric substance such as protein is electrically zero because the concentration of its cation form equals the concentration of its anion form. (pI). It offers several advantages over standard pre-fractionation methods, including enhanced ease of use, greater resolution (up to 0.1 pH units), analytical-to-preparative sample loading capacity, and the ability to run multiple samples in parallel. Unlike standard gel-based pI fractionation, the OFFGEL system streamlines the proteomics workflow by yielding fractions in solution, which can be directly subjected to further fractionation or LC/MS analysis. The ability to fractionate frac·tion·ate tr.v. frac·tion·at·ed, frac·tion·at·ing, frac·tion·ates 1. To divide or separate into parts; break up: samples by pI also allows researchers to target and validate the proteins or peptides of greatest interest, such as those that have undergone post-translational modifications. Using Agilent's Spectrum Mill software, scientists can incorporate pI data in their searches. The Agilent 3100 OFFGEL Fractionator can be incorporated into any proteomics workflow. For intact protein research, it can be used prior to 2D-gel electrophoresis to reduce sample complexity and improve resolution and sensitivity. For peptide analysis, it can be used before or in place of strong cation exchange. Used in conjunction with Agilent's Multiple Affinity Removal System and/or Macroporous Reversed-phase C18 column prior to LC/MS, it improves the sensitivity and productivity of both fast-scanning and accurate-mass analyses. The OFFGEL Fractionator system includes the instrument, trays, consumables and a local controller. For more information, visit Agilent online at www.agilent.com/chem/offgel. Availability The Agilent 3100 OFFGEL Fractionator is expected to be available for order starting July 1, 2006, with shipment beginning in October. About Agilent in Mass Spectrometry Agilent is a leading provider of GC/MS GC/MS Gas Chromatograph/Mass Spectrometer GC/MS Gas Chromatograph/Mass Spectrometry GC/MS Gas Chromatograph/Mass Spectrograph , LC/MS and ICP-MS ICP-MS Inductively Coupled Plasma Mass Spectroscopy technologies for organic and inorganic spectroscopy. Among the company's key products are the 5973 and 5975 inert GC/MSDs, the 7500 Series ICP-MS systems, and the new Agilent 6000 Series LC/MS single-quadrupole, triple-quadrupole, ion trap, TOF (Top Of Form) The beginning of a physical paper form. To position paper in many printers, the printer is turned offline, the forms are aligned properly and the TOF button is pressed. and Q-TOF instruments. Agilent provides innovative HPLC-Chip/MS technology and the broadest range of ion sources in the industry. As a leader in separations and software, Agilent can address the entire customer workflow from sample introduction through data analysis. About Agilent Technologies Agilent Technologies Inc. (NYSE:A) is the world's premier measurement company and a technology leader in communications, electronics, life sciences and chemical analysis. The company's 20,000 employees serve customers in more than 110 countries. Agilent had net revenue of $5.1 billion in fiscal 2005. Information about Agilent is available on the Web at www.agilent.com. NOTE TO EDITORS: The "I" in "pI" referenced above should be italicized. Press releases, photography (Image 86 in Image Library) and other information can be accessed on the Agilent Life Sciences and Chemical Analysis newsroom at www.agilent.com/about/newsroom/lsca. Further technology, corporate citizenship and executive news is available on the Agilent news site at www.agilent.com/go/news. |
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