Simplify and Accelerate Cloning.Promega Introduces Thermosensitive Alkaline Phosphatase alkaline phosphatase /al·ka·line phos·pha·tase/ (ALP) (fos´fah-tas) an enzyme that catalyzes the cleavage of orthophosphate from orthophosphoric monoesters under alkaline conditions. MADISON, Wis. -- Scientists can now save time and simplify the cloning process with the new Promega Thermosensitive Alkaline Phosphatase (TSAP TSAP - Transport Service Access Point ). For dephosphorylation of no DNA DNA: see nucleic acid. DNA or deoxyribonucleic acid One of two types of nucleic acid (the other is RNA); a complex organic compound found in all living cells and many viruses. It is the chemical substance of genes. in cloning reaction, scientists get both speed and simplicity because two unique features found in TSAP: 1. TSAP is active in all restriction enzyme restriction enzyme Protein (more specifically, an endonuclease) produced by bacteria that cleaves DNA at specific sites along its length. Thousands have been found, from many different bacteria; each recognizes a specific nucleotide sequence. buffers tested. That eliminates cleanup and buffer swaps. Most importantly, it allows restriction and dephosphorylation in the same reaction at the same time. 2. TSAP is irreversibly inactivated inactivated rendered inactive; the activity is destroyed. inactivated viruses treated so that they are no longer able to produce evidence of growth or damaging effect on tissue. by heating at 74xC for 15 minutes. This allows streamlining of the restriction enzyme digestion, desphosphorlyation and ligation ligation /li·ga·tion/ (li-ga´shun) the application of a ligature. tubal ligation sterilization of the female by constricting, severing, or crushing the uterine tubes. procedure by eliminating the need of cleanup after alkaline phosphatase treatment. As a quality product, TSAP is blue/white cloning-qualified. Scientists get a higher level of quality control for enzymes used in cloning applications. TSAP catalyzes the removal of 5' phosphate groups from DNA, thus preventing the recircularization and religation of linearized cloning vector DNA during ligation. It is effective on 3' and 5' overhangs and on blunt ends. Promega catalog number M9910 contains 100 reactions of TSAP. For more information or to request a sample visit: www.promega.com/tsap/ Images of data available upon request. About Promega Promega Corporation is a leader in providing innovative solutions and technical support to the life sciences industry. The company's 1,450 products enable scientists worldwide to advance their knowledge in genomics, proteomics, cellular analysis, molecular diagnostics and human identification. Founded in 1978, the company is headquartered in Madison, WI, USA with branches in 12 countries and 54 global distributors. Annual sales exceed $190 million. For more information about Promega, visit www.promega.com. |
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