Printer Friendly
The Free Library
22,728,043 articles and books

Seasonal shell growth and longevity in Donax variabilis from Northeastern Florida: evidence from oxygen isotopes.



ABSTRACT The variable coquina coquina

Limestone formed almost entirely of sorted and cemented fossil debris, most commonly coarse shells and shell fragments. Microcoquinas are similar sedimentary rocks composed of finer material.
 clam, Donax variabilis, is one of the most common inhabitants
:This article is about the video game. For Inhabitants of housing, see Residency
Inhabitants is an independently developed commercial puzzle game created by S+F Software. Details
The game is based loosely on the concepts from SameGame.
 of exposed sandy beach Sandy Beach (location ) is on the South Shore of Oʻahu in Hawaiʻi. It is known for its shorebreak for bodyboarding and bodysurfing. The area is also known for its strong current and dangerous shorebreak.  intertidal in·ter·tid·al  
adj.
Of or being the region between the high tide mark and the low tide mark.



in
 and shallow subtidal zones in the southeastern United States United States, officially United States of America, republic (2005 est. pop. 295,734,000), 3,539,227 sq mi (9,166,598 sq km), North America. The United States is the world's third largest country in population and the fourth largest country in area. . Its exceptional burrowing and migratory behaviors are well documented, as are its biogeograpbic distribution and phylogenetic phy·lo·ge·net·ic
adj.
1. Of or relating to phylogeny or phylogenetics.

2. Relating to or based on evolutionary development or history.
 relationships. However, basic life history parameters such as season and rate of shell growth and longevity are poorly constrained for this species, usually estimated from size-frequency analyses of sample populations. High-resolution sampling of individual D. variabilis shells, and analysis of the oxygen isotopic variation in these samples, provides an alternate method of assessing shell growth and longevity in this species with a high degree of precision. Comparison of isotopic paleotemperature profiles with local seawater seawater

Water that makes up the oceans and seas. Seawater is a complex mixture of 96.5% water, 2.5% salts, and small amounts of other substances. Much of the world's magnesium is recovered from seawater, as are large quantities of bromine.
 temperatures in the northeastern Florida study region indicates rapid shell growth (>4 mm/month) during a life span of 3 to 5 months, substantially shorter than most previous estimates. Detailed analysis of two modern shells indicates growth during spring and summer whereas four Archaic period The name Archaic Period is given by archaeologists to the earliest periods of a culture. In particular, it may refer to:
  • the Archaic period in the Americas (8000 BC–1000 BC)
  • the Archaic period in Greece (1000 BC–500 BC)
 archaeological shells revealed a summer-autumn growth record. In all cases the largest archaeological shells were substantially bigger than the largest modern shells, reflecting a greater longevity by about 2 months. Although recruitment can be fairly continuous throughout the year, size-frequency analyses of D. variabilis in separate years at the same locality reveal substantial interannual differences in population dynamics Population dynamics is the study of marginal and long-term changes in the numbers, individual weights and age composition of individuals in one or several populations, and biological and environmental processes influencing those changes. .

KEY WORDS: Donax variabilis, oxygen isotopes, shell growth, longevity, Florida

INTRODUCTION

The variable coquina clam, Donax variabilis Say, 1822, is one of the most common, recognizable bivalve bivalve, aquatic mollusk of the class Pelecypoda ("hatchet-foot") or Bivalvia, with a laterally compressed body and a shell consisting of two valves, or movable pieces, hinged by an elastic ligament.  mollusks from sandy beach habitats of the southeastern United States. It is found from Virginia to southern Florida and around the Gulf Coast to Texas (Ruppert & Fox 1988). Like other members of the Family Donacidae, D. variabilis is often the dominant macro-invertebrate inhabiting the high-energy environment of exposed sandy beaches where it often occurs in extremely high densities (Ansell 1983, Brown & McLachlan 1990, Pearse et al. 1942, Wilson 1999). In some cases Donax clams account for over 95% of the macrofaunal biomass on such beaches (McLachlan et al. 1981).

Donax clams are excellent burrowers, ideally suited to life in the surf zone surf zone
n.
See breaker zone.



surf zone

See breaker zone.


The area of water from the surf line to the beach. See also surf line.
 (McLachlan & Young 1982, Trueman & Ansell 1969). The wedge-shaped outline of their shells facilitates penetration into the substrate. In addition to rapid burrowing, D. variabilis is also noted for its mobility, moving up and down the beach with the tides (Turner & Belding 1957), responding in a complex manner to wave action and especially to the acoustic shock The term acoustic shock is used to describe the symptoms a person may experience after hearing an unexpected, loud sound via a telephone. The loud sound, called an Acoustic Incident  of breaking waves (Ellers 1995a, Ellers 1995b). On longer time scales, Ruppert and Fox (1988) report a seasonal cycle of migration across the beach zone, clown into the shallow sublittoral sub·lit·to·ral  
adj.
1.
a. Of or situated near the seashore.

b. Of or relating to an organism living near or just below the low tide level of a shore.

2.
 in fall, and returning onto the beach as juveniles in the late winter. This rather nomadic See nomadic computing.  lifestyle, combined with its supreme ability as a rapid burrower, has enabled this species to colonize col·o·nize  
v. col·o·nized, col·o·niz·ing, col·o·niz·es

v.tr.
1. To form or establish a colony or colonies in.

2. To migrate to and settle in; occupy as a colony.

3.
 the sands of exposed beaches that otherwise are fairly devoid of macrofauna (Wilson 1999).

Despite its familiarity to scientists and shell collectors alike, the taxonomic status of D. variabilis has been debated for decades. Morrison (1971) envisioned 2 largely sympatric sym·pat·ric  
adj. Ecology
Occupying the same or overlapping geographic areas without interbreeding. Used of populations of closely related species.
 species, D. variabilis and Donax parvula Philippi, 1849, with the former further subdivided into a subspecies subspecies, also called race, a genetically distinct geographical subunit of a species. See also classification.  occurring in the southwestern portion of the range, D. variabilis roemeri Philippi, 1849. By contrast, Abbott (1974) and Dance (1990) considered D. parvula an eco-morph of D. variabilis. From studies of morphology, genetics and ecology, Nelson et al. (1993) concluded that D. variabilis and D. parvula were highly similar, but distinct species.

Using molecular techniques (RAPD RAPD Randomly Amplified Polymorphic DNA
RAPD relative afferent pupillary defect (ophthalmology; aka Marcus-Gunn Pupil) 
 DNA DNA: see nucleic acid.
DNA
 or deoxyribonucleic acid

One of two types of nucleic acid (the other is RNA); a complex organic compound found in all living cells and many viruses. It is the chemical substance of genes.
 markers), Adamkewicz and Harasewych (1996) seem to have resolved the lingering systematics systematics: see classification.  issues as well as simplified our understanding of the biogeography Biogeography

A synthetic discipline that describes the distributions of living and fossil species of plants and animals across the Earth's surface as consequences of ecological and evolutionary processes.
 of Donax in eastern North America North America, third largest continent (1990 est. pop. 365,000,000), c.9,400,000 sq mi (24,346,000 sq km), the northern of the two continents of the Western Hemisphere. . They found no evidence for the existence of the subspecies D. variabilis roemeri and found D. parvula to be indistinguishable from the subtidal Donaxfossor Say, 1822, with the latter synonym having taxonomic priority. Their biogeographic bi·o·ge·og·ra·phy  
n.
The study of the geographic distribution of organisms.



bio·ge·og
 assessment has D. variabilis sharing both the Atlantic and Gulf coasts with a smaller, subtidal species, D. fossor on the Atlantic coast and Donax texasianus Philippi, 1847, in the Gulf of Mexico Noun 1. Gulf of Mexico - an arm of the Atlantic to the south of the United States and to the east of Mexico
Golfo de Mexico

Atlantic, Atlantic Ocean - the 2nd largest ocean; separates North and South America on the west from Europe and Africa on the east
. The Florida peninsula separates the two subtidal species (Adamkewicz & Harasewych 1996).

The principal distinguishing feature between D. variabilis and D. fossor/D, parvula is the angle of the dorsal margins of the shell on either side of the umbo umbo /um·bo/ (um´bo) pl. umbo´nes   [L.]
1. a rounded elevation.

2. the slight projection at the center of the outer surface of the tympanic membrane.


um·bo
n.
 (Wilson 1999). Unfortunately, juvenile specimens often cannot be assigned to one species or the other (e.g., Bonsdorff & Nelson 1992) and ecophenotypic variation can further complicate specific determination, particularly in the field. Hence, we considered all small shells ([less than or equal to] 3 mm) collected for this study to be D. variabilis, combining them with the larger specimens with which they were collected.

Despite its ecological importance, abundance and visibility on exposed sandy beaches, several key life history parameters of D. variabilis such as growth rate and longevity are known only to a first approximation 1. to a first approximation - When one is doing certain numerical computations, an approximate solution may be computed by any of several heuristic methods, then refined to a final value.  with widely divergent estimates in the literature. This is particularly significant when D. variabilis is used as a model organism A model organism is a species that is extensively studied to understand particular biological phenomena, with the expectation that discoveries made in the organism model will provide insight into the workings of other organisms.  in ecological studies involving population dynamics and energy budgets on sandy beaches (Wilson 1999), or in investigations of the roles of biotic biotic /bi·ot·ic/ (bi-ot´ik)
1. pertaining to life or living matter.

2. pertaining to the biota.


bi·ot·ic
adj.
1. Relating to life or living organisms.
 versus abiotic a·bi·ot·ic  
adj.
Nonliving: The abiotic factors of the environment include light, temperature, and atmospheric gases.



a
 factors in determining spatial distribution and recruitment of beach fauna (Schoeman & Richardson 2002). Longevity estimates for D. variabilis range from less than 1 year (e.g., Bonsdorff & Nelson 1992) to higher than 3 years (e.g., Morrison 1971). At least part of the explanation for the lack of accurate data is the traditional method for assessing growth and longevity in this species--analysis of size-frequency diagrams--an approach with many inherent problems (Mikkelsen 1985). Other techniques such as mark-and-recapture studies are impractical because of the movements and high mortality of these clams (Mikkelsen 1985), and growth rings on the shell are indistinct in·dis·tinct  
adj.
1. Not clearly or sharply delineated: an indistinct pattern; indistinct shapes in the gloom.

2. Faint; dim: indistinct stars.

3.
, not useful for age determination (Wilson 1999). In this study we use oxygen isotopic variation in shell carbonate to produce high-resolution records of seasonal shell growth and longevity in D. variabilis.

Florida coquina clams normally are not exploited as a food resource by humans today; however, archaeological sites in northeastern Florida attest to the fact that preColumbian people consumed vast quantities of coquina clams between the middle Archaic (ca. 5700 y BP) and St. Johns (ca. 400 y BP) periods (Milanich 1994). This study of growth and longevity in D. variabilis originated from an investigation of archaeological specimens recovered from coastal shell middens in northeastern Florida to determine if there was a seasonality to ancient shellfish harvest (Jones et al. 2003, Quitmyer et al. 2004). In addition to examining archaeological shells, year-round collections of living coquina clams were made to establish a modern analogue for comparative purposes. Oxygen isotopic profiles of both modern and archaeological shells document rapid seasonal growth and shorter life spans than previously acknowledged for this diminutive but important bivalve species.

MATERIALS AND METHODS

The earliest evidence for human use of marine resources in northeastern Florida comes from the many Archaic period coastal archaeological sites recognized from this region (Milanich 1994). Particularly abundant in these shell middens are specimens of D. variabilis that often dominate the zoo archaeological component of any particular site. Collections of D. variabilis have been made at many of the more prominent excavation sites by archaeologists and students from the University of Florida University of Florida is the third-largest university in the United States, with 50,912 students (as of Fall 2006) and has the eighth-largest budget (nearly $1.9 billion per year). UF is home to 16 colleges and more than 150 research centers and institutes.  (Quitmyer et al. 2004). These are housed in the collections of the Environmental Archaeology Environmental archaeology is the study of the long-term relationship between humans and their environments. Various sub-disciplines are involved to document and interpret this relationship, including paleoethnobotany, geomorphology, palynology, geophysics, landscape archaeology,  Program at the Florida Museum of Natural History The Florida Museum of Natural History is located at the University of Florida campus in Gainesville, Florida, USA. It displays exhibits on the flora, fauna, and people of Florida. The main museum is free of charge (but requests a donation). , University of Florida, Gainesville.

Four archaeological shells of D. variabilis were selected for oxygen isotopic analysis from among the largest specimens in the museum collections. Large specimens were chosen to increase the likelihood of measuring maximum longevity. Two well-preserved shells from the Preceramic pre·ce·ra·mic  
adj.
Of or being a society or culture prior to the use of ceramics or pottery.
 Archaic were selected, one each from the Crescent Beach Crescent Beach is the name of 6 places:
  • Crescent Beach, Florida
  • Crescent Beach, Michigan
  • Crescent Beach, Simcoe County, Ontario
  • Crescent Beach, York Regional Municipality, Ontario
  • Crescent Beach, Lunenburg County, Nova Scotia
 (4240 [+ or -] 80 corr. [sup.14]C yr BP) and Spencer's Midden midden

dungheap.
 (5570 [+ or -] 80 corr. [sup.14]C yr BP) sites (Fig. 1). The shell lengths of these specimens were 23.1 mm and 21.5 mm, respectively. In addition, two well-preserved specimens were selected from the Orange Period Archaic material, one shell (21.3 mm) from the Rollins Site (3760 [+ or -] 60 corr. [sup.14]C yr BP) and another (20.5 mm) from the Guana Shell Ring (3600 [+ or -] 50 corr. [sup.14]C yr BP).

[FIGURE 1 OMITTED]

To establish a modern analogue for comparison with the archaeological coquina shell material, D. variabilis specimens were live-collected at monthly intervals at Matanzas Beach between December 2001 to November 2002. The collection site on Anastasia Island For other uses, see Anastasia.
Anastasia Island is an island off the northeast Atlantic coast of Florida in the United States. The island is located east and southeast of St. Augustine. Part of the island (the Davis Shores and Lighthouse Park neighborhoods) are within St.
 is located just south of the beach access ramp from Fort Matanzas National Monument Fort Matanzas National Monument: see Saint Augustine, Fla.; National Parks and Monuments, table.
Fort Matanzas National Monument

National reserve, northeastern Florida, U.S.
 (N 29.7174[degrees]; W 81.2310[degrees]), north of the Matanzas Inlet Matanzas Inlet is a channel in Florida between barrier islands connecting the Atlantic Ocean and the south end of the Matanzas River. It is 14 miles south of St. Augustine, in the southern part of St. Johns County, at coordinates . , in the same vicinity and water depths where indigenous people could have harvested coquina clams thousands of years ago (see Fig. 1). Specimens were collected in the intertidal and shallow subtidal zones ([less than or equal to] 1.5-m depth) for a consistent 40-min interval each month. Live clams were water sieved from bulk samples scooped from the sandy bottom using a 1.59 mm-gauge brass sediment sieve, identical to that used to sieve archaeological materials. The clams were bagged, stored in a cooler, and transported back to the museum where they were quickly frozen and stored. Soft tissues were removed by water maceration mac·er·a·tion
n.
1. Softening by soaking in a liquid.

2. Softening of the tissues after death by autolysis, especially of a stillborn fetus.
 at a later date.

A comparable assemblage of D. variabilis shells, live-collected at monthly intervals between January to December 1988 at the same locality, was also available at the museum. These shells were collected by Dr. Michael Russo during the course of research in the Department of Anthropology at the University of Florida (Quitmyer et al. 2004).

The maximum shell length, anterior to posterior, was measured for each shell in every monthly collection from both data sets using a dial caliper caliper

Instrument that consists of two adjustable legs or jaws for measuring the dimensions of material parts. Spring calipers have an adjusting screw and nut; firm-joint calipers use friction at the joint to hold the legs unmoving.
. The monthly shell length frequencies were calculated and plotted in 1-mm increments to facilitate the temporal comparison of shell size variation in the populations. Similar size-frequency data were constructed by Quitmyer et al. (2004) for archaeological coquina clam shells excavated from middens in northeastern Florida. Two of the largest modern shells were selected for stable isotopic analysis (13.1 mm, 13.2 mm). These specimens were collected by Russo in July 1988.

When carefully sampled in ontogenetic on·to·ge·net·ic
adj.
Of or relating to ontogeny.
 sequence from the umbo to the ventral ventral /ven·tral/ (ven´tral)
1. pertaining to the abdomen or to any venter.

2. directed toward or situated on the belly surface; opposite of dorsal.


ven·tral
adj.
 shell margin (Fig. 2), the pattern of oxygen isotopic variation across a bivalve shell can provide a wealth of information about seasonal growth, shell growth rates Growth Rates

The compounded annualized rate of growth of a company's revenues, earnings, dividends, or other figures.

Notes:
Remember, historically high growth rates don't always mean a high rate of growth looking into the future.
, longevity, and season of death (Jones & Quitmyer 1996, Jones et al. 1983, Wefer & Berger 1991). To assess these parameters in D. variabilis, and to look at possible changes through time, sequential samples of shell carbonate were recovered from the two modern and four archaeological shells.

[FIGURE 2 OMITTED]

Prior to sampling each valve was cleaned following Andrus and Crowe (2002), with a wire brush wire brush nbrosse f métallique

wire brush wire nDrahtbürste f

wire brush n
 under distilled water Noun 1. distilled water - water that has been purified by distillation
H2O, water - binary compound that occurs at room temperature as a clear colorless odorless tasteless liquid; freezes into ice below 0 degrees centigrade and boils above 100 degrees centigrade;
 and then treated with a 30% solution of [H.sub.2][O.sub.2]. After rinsing in distilled water and drying in a vacuum oven A vacuum oven is a sealed chamber in which the pressure is lowered and the temperature is raised. One use of such an oven is to remove volatiles and bound gases from surfaces. Another is to heat a substance in an oxygen-poor environment to reduce oxidation. , the samples were mounted onto glass slides with epoxy and fixed to the sample stage of a Merchantek EO Micromill at the Savannah River Savannah River

River, eastern Georgia, U.S. Formed by the confluence of the Tugaloo and Seneca rivers at Hartwell Dam, it flows southeast to form the boundary between Georgia and South Carolina. It empties into the Atlantic Ocean at Savannah after a course of 314 mi (505 km).
 Ecology Laboratory. Carbonate samples were milled from the outer surface of the valve in shallow, 25 [micro]m-deep grooves parallel to the growth lines. Each sampling groove was approximately 100-[micro]m wide. The spacing between adjacent grooves averaged 200 [micro]m with grooves near the ventral margin slightly more closely spaced than those near the umbo, reflecting the slowing of shell growth with age (see Fig. 2).

The isotopic analyses were conducted in the Light Stable Isotope stable isotope
n.
An isotope of an element that shows no tendency to undergo radioactive breakdown.
 Mass Spectrometry mass spectrometry
 or mass spectroscopy

Analytic technique by which chemical substances are identified by sorting gaseous ions by mass using electric and magnetic fields.
 Laboratory, Department of Geological Sciences, University of Florida. The powdered CaC[O.sub.3] samples were analyzed according to according to
prep.
1. As stated or indicated by; on the authority of: according to historians.

2. In keeping with: according to instructions.

3.
 standard techniques (Jones & Quitmyer 1996) which involved an initial reaction in vacuo in vacuo /in vac·uo/ (vak´u-o) [L.] in a vacuum.  with 100% orthophosphoric acid orthophosphoric acid /or·tho·phos·phor·ic ac·id/ (-fos-for´ik) phosphoric acid.

or·tho·phos·phor·ic acid
n.
See phosphoric acid.
 at 90[degrees]C for 0.25 h. An on-line automated carbonate-preparation system facilitated the production and purification of the evolved C[O.sub.2] gas. The isotopic differences between the derived C[O.sub.2] gas and the VPDB VPDB Vienna PeeDee Belemnite  standard were determined with a VG Isogas PRISM Series I mass spectrometer equipped with triple collectors and mico-inlet system. All values are reported in standard 8 notation where:

[delta][sup.18]O=[[([sup.18]O/[sup.16]O).sub.sample]/[([sup.18]O/ [sup.16]O).sub.standard]-1] x [10.sup.3] per mil per mil also per mill
adv.
Per thousand.



[per + mil (short for Latin m
([per thoudand])

The weight of the individual microsamples was so low that replicates of unknowns could not be run and often required that adjacent samples be combined. However, replicated standards run before and after sample strings varied by less than [+ or -] 0.1[per thousand].

The [delta]][sup.18]O value of seawater from the Matanzas Beach collection site was measured via C[O.sub.2] equilibration equilibration /equi·li·bra·tion/ (e-kwil?i-bra´shun) the achievement of a balance between opposing elements or forces.

occlusal equilibration
 following Socki et al. (1992) at the University of Georgia Organization
The President of the University of Georgia (as of 2007, Michael F. Adams) is the head administrator and is appointed and overseen by the Georgia Board of Regents.
, Geology Stable Isotope Laboratory. Five monthly samples were analyzed (December to March & August), providing a measure of seasonal [delta][sup.18]O range. Precision was estimated based on analysis of the laboratory working standard of Athens tap water to be [+ or -]0.05 (1[sigma]). The [delta][sup.18]O values of seawater at the Matanzas Beach collection site were very consistent (Dec. 1.0[per thousand]; Jan. 1.2[per thousand]; Feb. 1.0[per thousand]; Mar. 1.1[per thousand]; Aug. 1.1[per thousand]), averaging 1.08[per thousand] SMOW SMOW Standard Mean Ocean Water
SMOW St. Mary Of The Woods (Chicago, Illinois) 
 ([+ or -] 0.08[per thousand], 1[sigma]: maximum range = 0.2[per thousand]).

We calculated the temperature of the water in which the shell carbonate formed using the paleotemperature equation of Grossman and Ku (1986) for the temperature-dependent fractionation fractionation /frac·tion·a·tion/ (frak?shun-a´shun)
1. in radiology, division of the total dose of radiation into small doses administered at intervals.

2.
 of aragonite aragonite

A carbonate mineral, the stable form of calcium carbonate (CaCO3) at high pressures. It is somewhat harder and has a slightly higher specific gravity than calcite.
 in mollusks relative to seawater:

T([degrees]C) = 21.8 - 4.69 [shell [delta][sup.18][O.sub.VPDB] - (seawater [delta][sup.18][O.sub.SMOW] - 0.2[per thousand])].

The paleotemperature data from the coquina shells are presented in ontogenetic sequence and graphed to the mean weekly seawater temperature curve based on historical measurements made in 1988 at the nearby St. Augustine National Data Buoy Center The National Data Buoy Center (NDBC), is a part of the National Oceanic and Atmospheric Administration's (NOAA) National Weather Service (NWS). NDBC designs, develops, operates, and maintains a network of data collecting buoys and coastal stations.  station, SAUFL (http://www.ndbc.noaa.gov/Maps/Florida.shtml).

RESULTS

At Matanzas Beach, 2,493 specimens of D. variabilis were collected in 1988 by Russo and measured as part of this study. Another 2,545 specimens were measured from the collection made in 2001 to 2002. This latter number represents the total of all specimens collected each month except April 2002 when 28,372 specimens were collected during the usual 40-min regimen. A subset of shells (344 specimens) equal in weight to the previous month's sample (March) was selected from the April 2002 sample for measurement. The monthly length-frequency relationships for each of these samples are illustrated in Figures 3 and 4.

[FIGURES 3-4 OMITTED]

Each of the monthly graphs for 1988 (see Fig. 3) reveals a concentration of specimens at the smaller sizes. During winter (December to February) most of the shells in the population ranged between 2-6 mm, reflecting a preponderance of new recruits entering the population. A small percentage of larger shells were observed, up to 12 mm. In the spring (March to May) clams were more abundant than during any other season. Most shell lengths ranged between 2-10 mm with peaks at 3, 5, and 7 mm, indicative of shell growth and continued recruitment. A few specimens in the May sample reached 14 mm. The summer quarter (June to August) was marked by a very strong peak at 3 mm indicating that recruitment remained vigorous. Shell lengths ranged between 2-13 mm. Although individuals were scarce compared with other seasons, the greatest proportion of large shells was encountered in summer (see Fig. 3). By autumn (September to November) the average shell length of the population had declined with a major influx of juvenile shells in the 2-5 mm range and a decrease in the percentage of large shells.

The monthly graphs for 2001 to 2002 (see Fig. 4) show similarities and significant differences from those of 1988. The winter months were characterized by specimens in the range of 1-11 mm. Peaks at 2, 4, and 5 mm in successive months reflect an influx of new recruits in December followed by individual shell growth and a concomitant increase in the average shell length throughout the winter. The continuation of this pattern during spring resulted in peaks at 3, 7, and 10 mm. Except for June 2002, the sample sizes in July and August were very small, insufficient for a robust interpretation of patterns. The autumn samples show a very strong peak at 3 mm and also some of the largest shells recovered in either year of collecting, up to 15 mm. Heavy recruitment is indicated as well as individual shell growth by older specimens.

High-resolution milling of the D. variabilis shells resulted in powdered CaC[O.sub.3] samples whose weights were toward the lower limits of those typically analyzed. Therefore, successive samples were combined to insure a sufficient sample size. With respect to the modern shells, this resulted in 8 unique samples for specimen #1 (13.1 mm) and 13 unique samples for specimen #2 (13.2 mm). Oxygen isotopic analyses of these samples produced values that ranged from 1.91[per thousand] to 0.03[per thousand] for specimen #1 and from 1.61[per thousand] to 0.83[per thousand] for specimen #2.

Figure 5 shows the mean weekly sea surface temperature Sea surface temperature (SST) is the water temperature at the surface. In practical terms, the exact meaning of "surface" will vary according to the measurement method used.  variation at Matanzas Beach for 1988 based on historical measurements made at the nearby St. Augustine National Data Center Buoy buoy (boi, b`ē), float anchored in navigable waters to mark channels and indicate dangers to navigation (isolated rocks, mine fields, cables, and the like).  station, SAUFL. The oxygen isotopic data from two shells collected in July 1988 were converted to paleotemperatures and overlain o·ver·lain  
v.
Past participle of overlie.
 in ontogenetic sequence onto the portion of the temperature curve that provided the best fit.

[FIGURE 5 OMITTED]

With the exception of only one data point, the isotopic paleotemperature data exhibit an excellent fit to the segment of the curve corresponding to spring and early summer. The exceptionally close match confirms the absence of vital effects influencing shell [delta][sup.18]O values in D. variabilis, as in most mollusks (Wefer & Berger 1991). The shell-edge paleotemperature values correspond nicely with the water temperature at the time of collection in early July 1988. The isotopic records indicate that shell growth in both specimens occurred over a 13-week interval, approximately corresponding to weeks 14 to 26 of the year (mid April to early July).

In a similar manner, the four larger archaeological shells were sampled and analyzed for oxygen isotopic ratios. A total of 18 samples was analyzed from the Crescent Beach specimen. The [delta][sup.18]O values ranged from 0.29[per thousand] to 1.55[per thousand]. From the Spencer's Midden shell a total of 20 samples was analyzed whose [delta][sup.18]O values ranged between 1.16[per thousand] and 1.37[per thousand]. A total of 20 samples from the Rollins Site shell yielded [delta][sup.18]O values that ranged between 1.26[per thousand] and 1.96[per thousand]. From the Guana Shell Ring specimen, 13 samples were analyzed. These produced [delta][sup.18]O values that ranged from 0.88[per thousand] to 1.49[per thousand].

The oxygen isotopic data were converted to paleotemperatures and these were overlain onto the seawater temperature curve (Fig. 6). The results are comparable with those achieved for the modern shells with a few important differences. The paleotemperature profiles for the two Preceramic Archaic shells from the Crescent Beach and Spencer's Midden sites show the closest correspondence with weeks 25 to 45 of the annual temperature curve. This pattern suggests shell growth occurred for about 21 weeks, from late June into mid-November. The paleotemperature profiles for the two younger shells from the Orange Period Archaic sites (Rollins and Guana Shell Ring) reveal a similar pattern. Shell growth over a period of about 20 weeks is indicated, corresponding to weeks 24 to 43 of the year, mid-June to late October.

[FIIGURE 6 OMITTED]

All four archaeological shells were larger in size than any of the modern shells collected as part of this study. The results indicate that the ancient clams achieved their larger size by growing for a longer period of time (i.e., 8-9 wk longer) than their modern counterparts. Whereas maximum longevity among the modern shells was approximately 3 months, the archaeological specimens lived for up to 5 months. The paleotemperature records indicate that each of the four archaeological shells was harvested in autumn. Although the seasonal pattern of paleotemperature change recorded by the oxygen isotopes in the archaeological shells mirrors that of modern seawater at this site, the values reveal a consistent negative offset from modern conditions. The paleotemperature values from both archaeological periods reflect ocean water conditions in this region that were warmer than modern temperatures by about 3.5[degrees]C.

DISCUSSION

Despite its abundance and familiarity to scientists and shell collectors, key life history parameters such as growth rate and longevity are poorly constrained for D. variabilis. As this short-lived but abundant bivalve finds increasing use in ecological modeling of exposed beach ecosystems, it is essential that basic life history parameters are determined more precisely.

The oxygen isotopic evidence from modern and archaeological shells of D. variabilis provides the most accurate assessment of seasonal shell growth and longevity in this species to date. These data serve to refine previous estimates that were based largely on size-frequency analyses that required critical assumptions to be made about the seasonal timing of recruitment and migrations, assumptions that may or may not be warranted. Inherent problems in the interpretation of life-history information from size-frequency data are acknowledged by several authors including Mikkelsen (1985, p. 310) who states, "... repeated sampling of the population to construct length-frequency graphs was used, although problems exist with its use."

In his overview of the biology of the genus Donax, Ansell (1983) reported that most species are short-lived with life spans of 1-2 y, rapid growth to maximum size, and early maturity. Morrison (1971) suggested that D. variabilis has a 2-year life span, and that in some cases individuals may survive a third year. Ansell (1983) reported a 1- to 2-year life cycle for D. variabilis. Mikkelsen (1985) estimated that D. variabilis in Florida grew at a rate of 3.0-3.7 mm/month in the summer months and that, "the majority of individuals probably live for approximately 1 year, with a few entering a second year" (Mikkelsen 1985, p. 310). This conclusion generally agrees with Loesch (1957) and Pearse et al. (1942) who also used length-frequency graphs to examine growth and longevity in this species.

"Bonsdorff and Nelson (1992) estimated a maximum growth rate of 3.43 mm/month for a Florida population of D. variabilis, at which rate the animals would grow to maturity in just 2 months" (Wilson 1999, p. 78). The oxygen isotope results achieved in this study support such rapid growth rate estimates and reduced longevity (i.e., months, not years). In fact, the growth rates calculated from our isotopic data are even higher, 4.3-4.4 mm/month for the modern specimens and 4.1-4.6 mm/month for the archaeological shells.

The isotopic data also call into question whether any of these clams actually survive for 1 full year as the modern and archaeological shell isotope records indicated life spans of 3 to 5 months, respectively. Admittedly both the modern and archaeological specimens were live-collected by humans and therefore it could be argued that they still had the potential to grow larger and live longer. However, both sets of shells were among the largest specimens out of the thousands recovered in either the modern or zooarchaeological collections, thereby minimizing this possibility. Our results cast serious doubt on previous longevity estimates for this species that hypothesize hy·poth·e·size  
v. hy·poth·e·sized, hy·poth·e·siz·ing, hy·poth·e·siz·es

v.tr.
To assert as a hypothesis.

v.intr.
To form a hypothesis.
 growth and survival into a second or even a third year of life.

In a study involving D. variabilis from the South Carolina South Carolina, state of the SE United States. It is bordered by North Carolina (N), the Atlantic Ocean (SE), and Georgia (SW). Facts and Figures


Area, 31,055 sq mi (80,432 sq km). Pop. (2000) 4,012,012, a 15.
 coast, Wilson (1999, p. 69) states: "Survival of Donax beyond a shell length of 10-11 ram, which length it can attain in less than 1 year, was poor and these large individuals only rarely contributed more than 1% to 2% of the population." He goes on to lament that growth rings on the shell were indistinct and it was not possible to verify the age of specimens by this method. Furthermore, constant movement of beach sands and contained clams, as well as the migratory ability of Donax variabilis, prevented the use of a "mark-and-recapture" technique for growth measurement. These same observations hold true regarding the populations in northeastern Florida and reinforce the value of the oxygen isotopic technique for growth rate and age determination.

The length-frequency graphs (see Figs. 3, 4) from the monthly collections of 1988 and 2001 to 2002 also reinforce Wilson's observation concerning the rarity of large individuals. The maximum SL in the modern Florida samples was 15 mm and such large shells were very rare. In contrast, the D. variabilis shells recovered from the archaeological shell midden sites ranged up to 25 mm and specimens in the 10-22 mm range were most common (Quitmyer et al. 2004). It seems clear from the zooarchaeological analyses that ancient harvesting strategies involved selection for larger shell sizes, but it is not clear why the coquina shells from the ancient past exceed the size of modern shells from the same vicinity.

The presence of small clams in the population virtually year-round suggests that recruitment to the population may take place throughout the year. This was especially noticeable in the 1988 samples (see Fig. 3) and has been mentioned as a possibility by previous authors (e.g., Bonsdorff & Nelson 1992). The pattern in 2001 to 2002 (see Fig. 4) was somewhat different with continuous recruitment indicated during September to March and weak or negligible recruitment over the spring and summer months. Leber (1982) recorded juvenile recruitment to a North Carolina North Carolina, state in the SE United States. It is bordered by the Atlantic Ocean (E), South Carolina and Georgia (S), Tennessee (W), and Virginia (N). Facts and Figures


Area, 52,586 sq mi (136,198 sq km). Pop.
 population occurring in February and November. The February settlement was indicative of a winter spawning. At the lower latitude of Florida, it seems reasonable that such winter spawnings could occur with greater regularity. The differences in the monthly shell size distributions between the 2 years sampled here suggest considerable year-to-year variability in the population dynamics at this northern Florida locality.

The population structure observed by Wilson (1999) at Waites Island, South Carolina, suggested an annual cycle of settlement and growth, with reproduction and death in spring, although questions of a second, lesser spawning in autumn remain. A similar pattern was evident in the Florida population of D. variabilis discussed by Bonsdorff and Nelson (1992). Two spawnings per year have been reported for D. sordidus (McLachlan & van der Horst 1979) and for D. semistratus (Neuberger-Cywiak et al. 1990), although a single spawning per year is the default condition reported or assumed for most other species of Donax, even the short-lived, tropical species. However, the evidence is somewhat equivocal as Ansell et al. (1972) suggest that individuals of species like D. incarnatus may be in spawning condition at all times. Wilson (1999) acknowledges that the same might be said for the Waites Island D. variabilis population, although he believed the majority of individuals were semelparous.

The size-frequency data collected as part of this study support the emerging realization that spawning and recruitment can vary considerably from 1 year to the next in D. variabilis and need not conform to the pattern of one major seasonal spawning per annum Per annum

Yearly.
. Such an interpretation agrees with Ansell' s (1983) observation that the typical spawning pattern for Donax consists of repeated spawnings by individuals over an extended spawning season. Following maturity, Ansell (1983) reports that reproduction is potentially continuous with repeated partial spawnings, but growth, reproduction, and settlement may all show seasonal responses to environmental change. Certainly our data from 1988 argue against the statement by Sastre (1984) that Donax denticulatus from Puerto Rico is the only known species of Donax with continuous recruitment.

Whereas the size-frequency analyses of the monthly samples provide important insights into the population dynamics of D. variabilis along the northeastern Florida coast, there are better methods available to investigate specific life history parameters such as age and growth rate. One such technique is the high-resolution sampling and analysis of oxygen isotopic variation in shell carbonate used here. This approach has yielded the most detailed records of seasonal shell growth and longevity available to date for this species. It is also applicable to archaeological and fossil shells. The excellent correspondence between paleotemperatures derived from oxygen isotopes and the measured seasonal variation in water temperature at the site made it possible to reconstruct the timing and duration of shell growth to a weekly level of resolution. Life spans that proved to be appreciably shorter than generally acknowledged in the literature, 3 to 5 months in duration, represent an unanticipated outcome of this study. Whereas budget constraints limited the number of shells analyzed isotopically in this study, it would be highly desirable to expand the size and geographic breadth of the sampling to broaden the scope of this investigation.

Several important differences were observed between the modern and archaeological D. variabilis shells. These are intriguing and warrant additional study as well. The questions arising from these observations can be grouped into 3 broad categories: archaeological, biologic, and climatic. With respect to the first, all four archaeological shell records, from distinct Archaic Period time horizons, indicated shellfish harvest in the autumn. This suggests a clear seasonal pattern of behavior by indigenous peoples that needs to be verified through the analysis of additional shells from other sites and time periods (Quitmyer et al. 2004).

The biologic differences include greater maximum size and longevity for the archaeological shells, an extended growing season, and a shift in the growing season from spring-early summer to mid summer-autumn. Isotopic analyses of additional modern and archaeological shells will be necessary to document the extent of these differences and gauge their changes through time. The possibility of size reduction from over-fishing, observed in many marine species (Jackson et al. 2001) and in other bivalves such as Mercenaria spp. from Florida midden sites (Quitmyer & Jones 2000), must be considered. Finally, the warmer water temperatures recorded by the archaeological shell isotopes (ca. 3.5[degrees]C) may reflect the midHolocene climatic maximum in this region (Jones et al. 2003). By attempting additional oxygen isotopic analyses of D. variabilis shells from older as well as younger archaeological time periods, it should be possible to chart the history of water temperature change in this region since the last glacial episode.

ACKNOWLEDGMENTS

The authors thank Dr. Michael Russo, National Park Service, Southeastern Center, Tallahassee, for sharing unpublished data in the support of this research. Jenna Chojnowsk, Catherine Liu, Nick Moskowitz, Kate Quitmyer, Erin Thornton, and Brian Worthington assisted with fieldwork, sample preparation, data acquisition, and curation. We acknowledge the Invertebrate Paleontology and Environmental Archaeology Laboratories, Florida Museum of Natural History, for supplying essential resources. US Department of Energy grant #DE-FC09-96SR18546 (CPTA CPTA California Physical Therapy Association
CPTA Canadian Property Tax Association
CPTA Council of Professional Teaching Associations
CPTA Center for Practice and Technology Assessment (AHRQ)
CPTA Canadian Paper Trade Association
) provided partial support of this research. We appreciate the analytical assistance given by Dr. Jason Curtis of the Light Stable Isotope Mass Spectrometry Laboratory, Department of Geological Sciences, University of Florida.

LITERATURE CITED

Abbott, R. T. 1974. American seashells, 2nd ed. New York New York, state, United States
New York, Middle Atlantic state of the United States. It is bordered by Vermont, Massachusetts, Connecticut, and the Atlantic Ocean (E), New Jersey and Pennsylvania (S), Lakes Erie and Ontario and the Canadian province of
: Van Nostrand Reinhold Company, 663 pp.

Adamkewicz, S. L. & M. G. Harasewych. 1996. Systematics and biogeography of the genus Donax (Bivalvia: Donacidae) in eastern North America. Am. Malacol. Bull. 13:97-103.

Andrus, C. F. T. & D. E. Crowe. 2002. Alteration of otolith otolith /oto·lith/ (o´to-lith) statolith.

o·to·lith
n.
1. Any of numerous minute calcareous particles found in the inner ear of certain lower vertebrates and in the statocysts of many
 aragonite: Effects of prehistoric cooking methods on otolith chemistry. J. Arch. Sci. 29:291-299.

Ansell, A. D. 1983. The biology of the genus Donax. In: A. McLachlan & T. Erasmus, editors. Sandy beaches as ecosystems. The Hague: W. Junk, pp. 607-635.

Ansell, A. D., P. Sividas, B. Narayanan & A. Trevallion. 1972. The ecology of two sandy beaches in south west India. III. Observations on the population of Donax incarnatus and D. spiculum spiculum /spic·u·lum/ (spik´u-lum) pl. spic´ula   [L.] spicule. . Mar. Biol. 17:318-332.

Bonsdorff, E. & W. G. Nelson. 1992. The ecology of coquina clams Donax variabilis Say, 1822, and Donax parvula Philippi, 1849, on the east coast of Florida. The Veliger ve·li·ger  
n.
A larval stage of a mollusk characterized by the presence of a velum.



[New Latin v
 35:358-365.

Brown, A. C. & A. McLachlan, editors. 1990. Ecology of sandy shores. Amsterdam: Elsevier. 328 pp.

Dance, S. P., editor. 1990. The collectors encyclopedia of shells. London: Zachary Kwintner Books Ltd. 288 pp.

Ellers, O. 1995a. Behavioral control of swash-riding in the clam Donax variabilis. BioL Bull. 189:120-127.

Ellers, O. 1995b. Discrimination among wave-generated sounds by a swash-riding clam. Biol. Bull. 189:128-137.

Grossman, E. L. & T.-L. Ku. 1986. Oxygen and carbon isotopic fractionation in biogenic biogenic /bi·o·gen·ic/ (-jen´ik) having origins in biological processes.

biogenic

having the property of originating in a biological process.
 aragonite: Temperature effects. Chem. Geol. 59:59-74.

Jackson, J. B. C., M. X. Kirby, W. H. Berger, K. A. Bjorndal, L. W. Botsford, B. J. Bourque, R. H. Bradbury, R. Cooke, J. Erlandson, J. A. Estes, T. P. Hughes, S. Kidwell, C. B. Lange, H. S. Lenihan, J. M. Pandolfi, C. H. Peterson, R. S. Steneck, M. J. Teqner, & R. R. Warner. 2001. Historical overfishing Overfishing occurs when fishing activities reduce fish stocks below an acceptable level. This can occur in any body of water from a pond to the oceans. More precise biological and bioeconomic terms define 'acceptable level'.  and the recent collapse of coastal ecosystems. Science 293:629-638.

Jones, D. S. & I. R. Quitmyer. 1996. Marking time with bivalve shells: Oxygen isotopes and season of annual increment formation. Palaios 11:340-346.

Jones, D. S., I. R. Quitmyer & C. F. T. Andrus. 2003. Stable isotope evidence of middle to late Holocene seasonality and temperature change in archaeological coquina clam shells from Florida. EOS Eos (ē`ŏs), in Greek religion and mythology, goddess of dawn; daughter of the Titans Hyperion and Theia. Every morning she arose early and preceded her brother Helios into the heavens. , Trans. Amer. Geophys. Un. 84(52), Ocean Sci. Mtg. Suppl., Abstr. OS511-08.

Jones, D. S., D. F. Williams & M. A. Arthur. 1983. Growth history and ecology of the Atlantic surf clam The Atlantic surf clam, Spisula solidissima, is a very large (20 cm), edible, saltwater clam or marine bivalve in the family Mactridae. It is a relative of the surf clam. , Spisula solidissima (Dillwyn), as revealed by stable isotopes and annual shell increments. J. Exp. Mar. Biol. Ecol. 73:225-242.

Leber, K. 1982. Bivalves (Tellinacea: Donacidae) on a North Carolina beach: contrasting population size structures and tidal migrations. Mar. Ecol. Prog. Ser. 7:297-301.

Loesch, H. C. 1957. Studies on the ecology of two species of Donax on Mustang Island, Texas. Publ. Inst. Mar. Sci. Univ. Texas 4:201-227.

McLachlan, A., T. Erasmus, A. H. Dye, T. Woolridge, G. van der Horst, G. Rossouw, T. A. Lasiak & L. Mcgwynne. 1981. Sand beach energetics en·er·get·ics  
n. (used with a sing. verb)
1. The study of the flow and transformation of energy.

2. The flow and transformation of energy within a particular system.
: an ecosystem approach towards a high-energy interface. Estuarine es·tu·a·rine  
adj.
1. Of, relating to, or found in an estuary.

2. Geology Formed or deposited in an estuary.

Adj. 1. estuarine - of or relating to or found in estuaries
estuarial
 Coastal Shelf Sci. 13:11-25.

McLachlan, A. & G. van der Horst. 1979. Growth and reproduction of two molluscs from an exposed sandy beach. S. Afr. J. Zool. 14: 194-201.

McLachlan, A. & N. Young. 1982. Effects of low temperature on the burrowing rates of four sandy beach molluscs. J. Exp. Mar. Biol. Ecol. 65:275-284.

Mikkelsen, P. S. 1985. A comparison of two Florida populations of the coquina clam, Donax variabilis Say, 1822 (Bivalvia: Donacidae). II. Growth rates. The Veliger 27:308-311.

Milanich, J. T. 1994. Archaeology of Precolumbian Florida. Gainesville: Florida University Press. 476 pp.

Morrison, J. P. E. 1971. Western Atlantic Donax. Biol. Soc. Wash. Proc. 83:545-568.

Nelson, W. G., E. Bonsdorff & L. Adamkewicz. 1993. Ecological, morphological, and genetic differences between the sympatric bivalves Donax variabilis Say, 1822, and Donax parvula Philippi, 1849. The Veliger 36:317-322.

Neuberger-Cywiak, L., Y. Achituv & L. Mizrahi. 1990. The ecology of Donax trunculus Linnaeus and Donax semistriatus Poli from the Mediterranean coast of Israel. J. Exp. Mar. Biol. Ecol. 134:203-220.

Pearse, A. S., A. J. Humm & G. W. Wharton. 1942. Ecology of sand beaches at Beaufort, North Carolina Beaufort (pronounced "BO-furt" / IPA: ˈbo.fɚt) is a town in Carteret County, North Carolina, United States. . Ecol. Monogr. 12:135-190.

Quitmyer, I. R. & D. S. Jones. 2000. The over-exploitation of hard clams (Mercenaria spp.) from five archaeological sites in the southeastern United States. Florida Anthropologist 53:160-167.

Quitmyer, I. R., D. S. Jones & C. F. T. Andrus. 2004. Seasonal collection of coquina clams (Donax variabilis Say, 1822) during the Archaic and St. Johns periods in coastal northeast Florida. In: D. Bar-Yosef, editor. Archaeomalacology: molluses in former environments of human behavior. London: Oxbow Press. In press.

Ruppert, E. E. & R. S. Fox. 1988. Seashore animals of the southeast. Columbia: University of South Carolina Press The University of South Carolina Press (or USC Press), founded in 1944, is a university press that is part of the University of South Carolina. External link
  • University of South Carolina Press


  
. 429 pp.

Sastre, M. P. 1984. Relationships between environmental factors and Donax denticulatus populations in Puerto Rico. Estuarine Coastal Shelf Sci. 19:217-230.

Schoeman, D. S. & A. J. Richardson. 2002. Investigating biotic and abiotic factors affecting the recruitment of an intertidal clam on an exposed sandy beach using a generalized additive model In statistics, the generalized additive model (or GAM) is a statistical model developed by Trevor Hastie and Rob Tibshirani blending properties of multiple regression (a special case of general linear model) with additive models. . J. Exp. Mar. Biol. Ecol. 276:67-81.

Socki, R. A., H. R. Karlsson & E. K. Gibson. 1992. Extraction technique for the determination of O-18 in water using preevacuated glass vials. Analyt. Chem. 64:829-831.

Trueman, E. R. & A. D. Ansell. 1969. The mechanism of burrowing into soft substrata by marine animals. Oceanogr. Mar. Biol. Ann. Rev. 7: 315-366.

Turner, H. J. & A. L. Belding. 1957. The tidal migration of Donax variabilis Say. LimnoL Oceanogr. 2:120-124.

Wefer, G. & W. H. Berger. 1991. Isotope paleontology paleontology (pā'lēəntŏl`əjē) [Gr.,= study of early beings], science of the life of past geologic periods based on fossil remains. : growth and composition of extant calcareous calcareous /cal·car·e·ous/ (kal-kar´e-us) pertaining to or containing lime; chalky.

cal·car·e·ous
adj.
 species. Mar. Geol. 100:207-248.

Wilson, J. G. 1999. Population dynamics and energy budget for a population of Donax variabilis (Say) on an exposed South Carolina beach. J. Exp. Mar. Biol. Ecol. 239:61-83.

DOUGLAS S. JONES, (1), * IRVY R. QUITMYER (1) AND C. FRED T. ANDRUS (2)

* Corresponding author. E-mail: dsjones@flmnh.ufl.edu

(1) Florida Museum of Natural History, University of Florida, Gainesville, Florida 32611 and

(2) University of Georgia, Savannah River Ecology Laboratory, Aiken, South Carolina Aiken is a city in Aiken County, South Carolina and is part of the CSRA. The population was 25,337 at the 2000 census. It is the county seat of Aiken CountyGR6, and is the site of the University of South Carolina at Aiken.  29802
COPYRIGHT 2004 National Shellfisheries Association, Inc.
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2004, Gale Group. All rights reserved. Gale Group is a Thomson Corporation Company.

 Reader Opinion

Title:

Comment:



 

Article Details
Printer friendly Cite/link Email Feedback
Author:Andrus, C. Fred T.
Publication:Journal of Shellfish Research
Geographic Code:1USA
Date:Dec 1, 2004
Words:6179
Previous Article:Microsatellite and allozyme analyses reveal few genetic differences among spatially distinct aggregations of geoduck clams.
Next Article:The effect of temperature on clearance rate and oxygen consumption of scallops, Chlamys farreri.
Topics:



Related Articles
Ice age sent shivers through the tropics.
Listening to fish for extinction clues.
Earful of data hints at ancient fish migration. (Paleobiology).
Regeneration time and morphology of the inhalant siphon of Donax denticulatus Linnaeus, 1758 (Bivalvia, Donacidae) after amputation.
The association between the coquina clam Donax fossor say and its epibiotic hydroid Lovenella gracilis clarke.
Growth rate and longevity of Dreissena polymorpha (Pallas): a review and recommendations for future study.
The hypoxic tolerance of the protobranch bivalve Nucula sulcata bronn.
Ageing and metabolism of Mytilus edulis: populations from various climate regimes.

Terms of use | Copyright © 2014 Farlex, Inc. | Feedback | For webmasters