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Screening assay for ethylene glycol in serum.

To the Editor:

The recent paper by Eder et al. (1) recalls, among other things, the problems that a clinical STAT laboratory has to face in the case of ethylene glycol poisoning. As an alternative to the precise and accurate gas chromatographic chro·mat·o·graph  
n.
An instrument that produces a chromatogram.

tr.v. chro·mat·o·graphed, chro·mat·o·graph·ing, chro·mat·o·graphs
To separate and analyze by chromatography.
 technique, the enzymatic assay with glycerol glycerol, glycerin, glycerine, or 1,2,3-propanetriol (prō`pāntrī'ŏl), CH2OHCHOHCH2OH, colorless, odorless, sweet-tasting, syrupy liquid.  dehydrogenase dehydrogenase /de·hy·dro·gen·ase/ (de-hi´dro-jen-as?) an enzyme that catalyzes the transfer of hydrogen or electrons from a donor, oxidizing it, to an acceptor, reducing it.

de·hy·dro·gen·ase
n.
 (EC 1.1.1.6) and NADH NADH the reduced form of NAD.

NADH
n.
The reduced form of NAD.


NADH,
n.pr a coenzyme that incorporates niacin and involved in the Krebs cycle.
 as tracer has been proposed by various authors [see, e.g., (2-4)]. The relative nonspecificity of glycerol dehydrogenase from Enterobacter aerogenes toward diols and triols allows interference with this test by propylene glycol, glycerol, 1,2-butanediol, and 2,3-butanediol (2, 5, 6). Nonetheless, its usefulness in emergency screening for ethylene glycol has been stressed, particularly for its technical simplicity (2).

Rynder et al. (2) used an analyzer, the Du Pont aca[R] SX (presently distributed by Dade Behring Inc., Deerfield, IL), that is particularly suited for emergency situations. Presently this instrument, together with the more recent version, the aca STAR, is still widely used in laboratories both in this country and in the United States. Unfortunately, the product that, following the method of Rynder et al., we were using to avoid interference from endogenous triacylglycerols and glycerol (triglyceride test kit for Monarch, cat. no. 35190, Instrumentation Laboratory) is no longer available in our country.

Because skilled personnel are not available at all times in our emergency laboratory and because cases of ethylene glycol poisoning are infrequent but not uncommon, I decided to continue to use the aca method whenever the gas chromatographic technique was not available. The modification I make is trivial: I cut compartment no. 2 (ingredients: lipase lipase (lī`pās), any enzyme capable of degrading lipid molecules. The bulk of dietary lipids are a class called triacylglycerols and are attacked by lipases to yield simple fatty acids and glycerol, molecules which can permeate the membranes  and stabilizers) of the aca triglyceride analytical test pack (Du Pont, cat. no. 702230901), wash it with deionized water, and firmly reseal reseal
Verb

to close or secure tightly again

Verb 1. reseal - seal again; "reseal the bottle after using the medicine"
seal, seal off - make tight; secure against leakage; "seal the windows"
 the hole with transparent adhesive tape. I calibrate To adjust or bring into balance. Scanners, CRTs and similar peripherals may require periodic adjustment. Unlike digital devices, the electronic components within these analog devices may change from their original specification. See color calibration and tweak.  the test with a 16 mmol/L solution of ethylene glycol in pooled sera that are free of ethylene glycol, as assessed by gas chromatographic analysis, and use the same pooled sera as a blank. Linearity was studied by serial dilutions of the calibrator in ethylene glycol-free pooled sera down to the concentration of 1.6 mmol/L. The linear regression analysis of the results gave the following statistics: dose 0.955 response 0.04 mmol/L, n = 10, r = 0.995, [S.sub.y|x] = 0.41 mmol/L. The test is recalibrated every month.

In our laboratory, we are aware that the results obtained in this way need cautious interpretation and gas chromatographic confirmation; however, this test permits us to screen economically for ethylene glycol with a typical turnaround time30 min.

References

(1.) Eder AF, McGrath CM, Dowdy YG, Tomaszewski JE, Rosemberg FM, Wilson RB. Ethylene glycol poisoning: toxicokinetic and analytical factors affecting laboratory diagnosis. Clin Chem 1998;44:168-77.

(2.) Rynder KW, Glick MR, Jackson SA. Emergency screening for ethylene glycol in serum. Clin Chem 1986;32:1574-7.

(3.) Hansonn P, Masson P. Simple enzymatic screening assay for ethylene glycol (ethan-1,2-diol) in serum. Clin Chim Acta 1989;182:95-102.

(4.) Standerfer J, Blackwell W. Enzymatic method for measuring ethylene glycol with a centrifugal analyzer. Clin Chem 1991;37:1734-6.

(5.) Nilsson L, Jones AW. 2,3-Butanediol: a potential interfering substance in the assay of ethylene glycol by an enzymatic method. Clin Chim Acta 1992;208:225-9.

(6.) Malandain H, Cano Y. Interferences of glycerol, propylene glycol, and other diols in the enzymatic assay of ethylene glycol. Eur J Clin Chem Clin Biochem 1996;34:651-4.

Francesco Zoppi

Laboratorio di Biochimica Clinica

ed Ematologia

Ospedale Niguarda Ca' Granda

Piazza Ospedale Maggiore 3

20162 Milan, Italy

Fax 39-2-64442901

E-mail marta.melotti@galactica.it

The Director of Regulatory Affairs and Quality Assurance for the Dade Behring Chemistry Group replies:

To the Editor:

As manufacturer of the aca[R] discrete clinical analyzer and the TGL TGL Taeglich (German: daily)
TGL Touch and Go Landing (aircraft flight training)
TGL Temporary Guidance Leaflets
TGL Technische Güte und Lieferbedingungen (German) 
 test pack described in this Letter to the Editor, Dade Behring appreciates the opportunity to comment. As described in our labeling, the intended use of the TGL test pack is to measure quantitatively the triglycerides in serum. Dade Behring cannot support non-standard use of the TGL test pack for ethylene glycol testing because the TGL test pack has not been optimized for this use. Furthermore, because we have not filed a premarket notification with the US Food and Drug Administration for use of TGL test packs in ethylene glycol testing, we are restricted from promoting or endorsing the product for this use.

Kay Maher

Chemistry Group

Dade Behring

Newark, DE 19714-6101

Fax 302-631-6299
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Title Annotation:Letters
Author:Zoppi, Francesco; Maher, Kay
Publication:Clinical Chemistry
Article Type:Letter to the editor
Date:Nov 1, 1998
Words:743
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