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Rickettsia parkeri infection after tick bite, Virginia.

We describe a man with a febrile illness and an eschar eschar /es·char/ (es´kahr)
1. a slough produced by a thermal burn, by a corrosive application, or by gangrene.

2. tache noire.

 that developed at the site of a tick bite. Rickettsia rickettsia (rĭkĕt`sēə), any of a group of very small microorganisms, many disease-causing, that live in vertebrates and are transmitted by bloodsucking parasitic arthropods such as fleas, lice (see louse), and ticks.  parked was detected and isolated from the eschar. This report represents the second documented case of R. parked rickettsiosis rickettsiosis /rick·ett·si·o·sis/ (ri-ket?se-o´sis) infection with rickettsiae.

Infection with Rickettsia bacteria.
 in a US serviceman in eastern Virginia.


In the United States, 4 species of spotted fever group (SFG) rickettsiae are recognized as pathogens of humans. These include Rickettsia rickettsii, the cause of Rocky Mountain spotted fever Rocky Mountain spotted fever, infectious disease caused by a rickettsia. The germ is harbored by wild rodents and other animals and is carried by infected ticks that attach themselves to humans.  (RMSF RMSF
Rocky Mountain spotted fever

RMSF, See Rocky Mountain spotted fever.
); R. felis, the cause of fleaborne spotted fever; R. akari, the agent of rickettsialpox; and R. parkeri (1,2). Of these, R. rickettsii is the only pathogen definitely associated with tick bites.

In 2004, Paddock et al. described the first recognized case of infection in a patient with R. parkeri (1). That patient, a US serviceman living in the Tidewater region of eastern Virginia, had a mild febrile illness and multiple eschars. He reported frequent tick and flea exposures but could not recall a specific arthropod arthropod

Any member of the largest phylum, Arthropoda, in the animal kingdom. Arthropoda consists of more than one million known invertebrate species in four subphyla: Uniramia (five classes, including insects), Chelicerata (three classes, including arachnids and horseshoe
 bite in the month before illness. However, R. parkeri, a tick-associated rickettsia (3), was subsequently isolated in cell culture from 1 eschar. (1) We present the second known case of spotted fever due to R. parkeri in a serviceman (the third case overall) and its unequivocal association with tick bite.

The Case

A 53-year-old US serviceman was seen at our clinic on September 8, 2006; he reported a 2-day history of fever, malaise, and rash. He denied headache, nausea, vomiting, or myalgia. He had recently returned from a vacation in the Virginia Beach area, where he had removed a large brown tick with white markings from his right pretibial region. The patient estimated that the tick had been attached [approximately equal to]8 hours before being removed. Four days after the tick was removed, an eschar developed at the bite site. Three days later, temperatures up to 39[degrees]C and drenching drenching

farmer's term for the administration of medicines as solutions or suspensions in water by mouth with a drench bottle, gun or funnel.

drenching bit
to be included in a bridle as a bit.
 night sweats that persisted for 2 days developed. He then sought care.

On examination, his temperature was 38[degrees]C. A non-tender, nonpruritic eschar that measured 1 cm x 1 cm was visible on his right lower extremity (Figure 1) along with [approximately equal to]15 nontender, nonpruritic papules Papules
Firm bumps on the skin.

Mentioned in: Smallpox
 on his torso, upper arms, and legs (Figure 2). The remainder of his physical examination was normal. Laboratory studies were normal except for a leukocyte count of 3.4 x [10.sup.9] cells/L (normal 4-11 x [10.sup.9] cells/L) and an aspartate aminotransferase level of 38 U/L (normal 8-33 U/L).


The patient was admitted to the hospital and treated with oral doxycycline 100 mg twice a day. Fevers and malaise immediately resolved after the first dose. The patient was discharged, and the rash resolved after 3-4 days of therapy.

DNA DNA: see nucleic acid.
 or deoxyribonucleic acid

One of two types of nucleic acid (the other is RNA); a complex organic compound found in all living cells and many viruses. It is the chemical substance of genes.
 extracted from skin-biopsy specimens of the proximal tibial eschar and a shoulder papule papule /pap·ule/ (pap´ul) a small, circumscribed, solid, elevated lesion of the skin.pap´ular

n. pl.
 was positive by 2 real-time PCR PCR polymerase chain reaction.

polymerase chain reaction

Polymerase chain reaction (PCR) 
 assays designed to amplify segments of the 17-kDa antigen and outer membrane protein B genes of all Rickettsia spp. and tickborne SFG rickettsiae, respectively (4,5). Amplicons obtained from 2 additional genes, gltA (333 bp) and sca4 (849 bp), were sequenced and determined to be 100% identical to those of R. parkeri (GenInfo Identifier nos. 1389996; 13568656). The histopathology of the shoulder papule showed mild superficial perivascular perivascular /peri·vas·cu·lar/ (-vas´ku-lar) near or around a vessel.


around a vessel.

perivascular cellulitis
 inflammation without eosinophils Eosinophils
A leukocyte with coarse, round granules present.

Mentioned in: Histiocytosis X

. Acute-phase serum and whole blood samples from the patient were also tested by real-time PCR and were negative. R. parkeri (Ft. Story strain) was isolated in Vero E6 cells from a portion of the eschar biopsy specimen. Immunohistochemical staining of the shoulder papule identified occasional spotted fever rickettsiae in the inflammatory cell infiltrate. Acute- and convalescent-phase serum specimens were tested for immunoglobulin G antibodies reactive with SFG and typhus group (TG) rickettsiae by using ELISAs with R. rickettsii and R. typhi antigens. The titers for the SFG ELISA ELISA (e-li´sah) Enzyme-Linked Immuno-Sorbent Assay; any enzyme immunoassay using an enzyme-labeled immunoreactant and an immunosorbent.

 acute- and convalescent-phase serum samples were <100 and 1,600, respectively. Both samples were negative (titer <100) for the TG ELISA.


R. parkeri was first isolated in 1937 from Amyblyomma maculatum ticks found on cattle in the Gulf Coast region of Texas (3), but its role as a human pathogen was unknown. For many years investigators speculated that agents other than R. rickettsii, including R. parkeri, caused mild RMSF-like illnesses in the United States (6,7). However, the role of R. parkeri as a pathogen of humans was not confirmed until 2002, when R. parkeri was isolated from a patient with a relatively mild febrile illness and multiple eschars (1). Confirmed cases of R. parkeri rickettsiosis have been described only twice (1,8), and the prevalence of this disease is unknown; however, many cases of this infection have likely been misidentified as RMSF (6). Recently, Raoult and Paddock analyzed serum specimens of 15 US patients who had an earlier diagnosis of RMSF and identified 4 that reacted with a 120-kDa protein of R. parkeri, a finding suggestive of infection with this agent (9). This hypothesis is further supported by seroprevalence studies of US military personnel that showed that 10% of those tested were positive for SFG rickettsiae when a R. riekettsii ELISA antigen was used (10). Because of recognized cross-reactivity of R. rickettsii with antigens of other SFG rickettsiae, including R. parkeri, this level of serologic reactivity could represent mild, self-limited infections with other SFG rickettsiae, including R. parkeri, that are less virulent than R. rickettsii.

Our patient had a single eschar at the site of tick attachment. Although it has been presumed that ticks are involved in the transmission of this disease, this is the first clear documentation of this occurrence. The infection can apparently be transmitted within several hours of attachment because the patient was certain of the maximum interval that the tick could have been attached to a visible region of his leg. Finally, the general description of the tick matches that of A. maculatum, the putative vector of R. parkeri rickettsiosis, and this tick has been collected previously in southeastern Virginia (11).

R. parkeri is the newest member of the SFG rickettsiae in the Western Hemisphere to be conclusively associated with illness in humans (12). Because 2 of the 3 patients described to date were US servicemen in the Virginia Tidewater region, where thousands of military personnel are stationed, this tickborne infection has potential military relevance. Serologic tests specifically for R. parkeri are not widely available, and an accurate diagnosis is best made by using PCR testing of a biopsy specimen of eschars or papules. A definitive diagnosis can also be made by using cell culture isolation techniques with the same biopsy specimen, as described for other eschar-associated rickettsioses Rickettsioses

Often severe infectious diseases caused by several diverse and specialized bacteria, the rickettsiae and rickettsia-like organisms. The best-known rickettsial diseases infect humans and are usually transmitted by parasitic arthropod vectors.

Clinicians should be aware of this newly noted rickettsial rickettsial /rick·ett·si·al/ (ri-ket´se-al) pertaining to or caused by rickettsiae.

Relating to, or caused by a member of the genus Rickettsia.
 illness, specifically in the eastern coastal region of the United States. They should consider this diagnosis for patients with mild rickettsiosis-like illness and single or multiple eschars.


(1.) Paddock CD, Sumner JW, Comer JA, Zaki SR, Goldsmith CS, Goddard J, et al. Rickettsia parkeri: a newly recognized cause of spotted fever rickettsiosis in the United States. Clin Infect Dis. 2004;38:805-11. Epub 2004 Mar 1.

(2.) Raoult D, Roux V. Rickettsioses as paradigms of new or emerging infectious diseases. Clin Microbiol Rev. 1997; 10:694-719.

(3.) Parker RR, Hohls GM, Cox GW, Davis GE. Observations on an infectious agent from Amblyomma maculatum. Public Health Rep. 1939;54:1482-4.

(4.) Blair PJ, Jiang J, Schoeler GB, Moron C, Anaya E, Cespedes M, et al. Characterization of spotted fever group rickettsiae in flea and tick specimens from northern Peru. J Clin Microbiol. 2004;42:4961-7.

(5.) Jiang J, Chan TC, Temenak JJ, Dasch GA, Ching WM, Richards AL. Development of a quantitative real-time polymerase chain reaction In Molecular Biology, real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction  assay specific for Orientia tsutsugamushi. Am J Trop Med Hyg. 2004;70:351-6.

(6.) Stothard DR, Fuerst PA. Evolutionary analysis of the spotted fever and typhus groups of Rickettsia using 16S rRNA gene sequences. Syst Appl Microbiol. 1995;18:52-61.

(7.) Walker DH, Fishbein DB. Epidemiology of rickettsial diseases. Eur J Epidemiol. 1991;7:237-45.

(8.) Finley RW, Goddard J, Raoult D, Eremeeva ME, Cox RD, Paddock CD. Rickettsia parkeri: a case of tick-borne, eschar-associated spotted fever in Mississippi. In: Program and Abstracts of the International Conference on Emerging Infectious Diseases The ICEID or International Conference on Emerging Infectious Diseases is a conference for public health professionals on the subject of emerging infectious diseases.  Atlanta, Georgia, 2006 Mar 19-22. Washington: American Society for Microbiology The American Society for Microbiology (ASM) is a scientific organization, based in the United States although with over 43,000 members throughout the world. It is the largest single life science professional organization and its members include those whose interests encompass basic ; 2006. [Abstract no. 188.]

(9.) Raoult D, Paddock CD. Rickettsia parkeri infection and other spotted fevers in the United States. N Engl J Med. 2005;353:626-7.

(10). Richards AL, Malone JD, Sheris S, Weddle JR, Rossi CA, Ksiazek TG, et al. Arbovirus arbovirus

Any of a large group of viruses that develop in arthropods (chiefly mosquitoes and ticks). The name derives from “arthropod-borne virus.” The spheroidal virus particle is encased in a fatty membrane and contains RNA; it causes no apparent harm to the
 and rickettsial infections among combat troops during Operation Desert Shield/Desert Storm. J Infect Dis. 1993;168:1080-1.

(11.) Sonenshine DE, Lamb JT Jr, Anastos G. The distribution, hosts and seasonal activity of Virginia ticks. Va J Sci. 1965;16:26-91.

(12.) Parola P, Paddock CD, Raoult D. Tick-borne rickettsioses around the world: emerging diseases challenging old concepts. Clin Microbiol Rev. 2005;18:719-56.

Address for correspondence: LCDR Timothy J. Whitman, US Navy infectious Diseases Department, National Naval Medical Center The National Naval Medical Center in Bethesda, Maryland, also known as the Bethesda Naval Hospital, is considered the flagship of the United States Navy's system of medical centers. , Bethesda, MD 20889 USA; email:

Timothy J. Whitman, * Allen L. Richards, ([dagger]) Christopher D. Paddock, ([double dagger]) Cindy L. Tamminga, ([dagger]) Patrick J. Sniezek, * Ju Jiang, ([dagger]) David K. Byers, * and John W. Sanders *

* National Naval Medical Center, Bethesda, Maryland, USA; ([dagger]) Naval Medical Research Center, Silver Spring, Maryland Not to be confused with Silver Springs.
Silver Spring is an urbanized, unincorporated area in Montgomery County, Maryland, USA. After Baltimore and Columbia, Silver Spring is the third most populous Census Designated Place in Maryland.
, USA; and ([dagger]) Centers for Disease Control and Prevention Centers for Disease Control and Prevention (CDC), agency of the U.S. Public Health Service since 1973, with headquarters in Atlanta; it was established in 1946 as the Communicable Disease Center. , Atlanta, Georgia, USA

Dr Whitman is an infectious disease specialist at the National Naval Medical Center and an assistant professor of medicine at the Uniformed Services University in Bethesda, Maryland, USA. His research is focused on community-associated methicillin-resistant Staphylococcus aureus methicillin-resistant Staphylococcus aureus Methicillin-aminoglycoside resistant Staphylococcus aureus, MRSA An organism with multiple antibiotic resistances–eg, aminoglycosides, chloramphenicol, clindamycin, erythromycin, rifampin, tetracycline, .
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Title Annotation:DISPATCHES
Author:Sanders, John W.
Publication:Emerging Infectious Diseases
Date:Feb 1, 2007
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