Reinforcement of an end-to-end tracheal resection anastomosis with fibrin glue: A case report.
Tracheal resection and primary anastomosis anastomosis /anas·to·mo·sis/ (ah-nas?tah-mo´sis) pl. anastomo´ses [Gr.]
1. communication between vessels by collateral channels.
2. is the treatment of choice for a short-segment stenosis. However, the procedure does carry the risk of two potentially fatal complications: anastomosis breakdown and leak. We describe the case of a 67-year-old man who was treated for a 3-cm tracheal stenosis secondary to a prolonged intubation intubation /in·tu·ba·tion/ (in?too-ba´shun) the insertion of a tube into a body canal or hollow organ, as into the trachea.
endotracheal intubation and multiple tracheostomies. The patient underwent a tracheal resection and primary anastomosis. The anastomosis was reinforced with fibrin sealant, which created an airtight seal. The patient was extubated postoperatively, and he healed without complication. Fibrin sealant is a convenient, safe, and effective material for reinforcing anastomotic suture lines.
Postintubation tracheal injury is the most common indication for tracheal resection and reconstruction. Common postoperative complications include suture-line granulation granulation /gran·u·la·tion/ (-shun)
1. the division of a hard substance into small particles.
2. the formation in wounds of small, rounded masses of tissue during healing; also the mass so formed. , dehiscence dehiscence /de·his·cence/ (de-his´ins) a splitting open.
wound dehiscence separation of the layers of a surgical wound.
n. of the anastomosis, restenosis, vocal fold paralysis, tracheomalacia, hemorrhage, and infection. The incidence of one of these complications--suture-line granuloma--has decreased since the use of absorbable synthetic sutures (e.g., Vicryl) and the submucosal submucosal /sub·mu·co·sal/ (-mu-ko´sal)
1. pertaining to the submucosa.
2. beneath a mucous membrane. placement of sutures has become more commonplace.  The incidence of the other complications has remained constant.
A 67-year-old man who experienced a tracheal stenosis after a prolonged intubation and tracheostomy decannulation came to the otolarynogology service with a complaint of stridor Stridor Definition
Stridor is a term used to describe noisy breathing in general, and to refer specifically to a high-pitched crowing sound associated with croup, respiratory infection, and airway obstruction. at rest. A head and neck examination revealed the presence of a well-healed tracheostomy scar. Flexible fiberoptic laryngoscopy revealed that both vocal folds were mobile. Computed tomography of the neck revealed a proximal tracheal stenosis. Flexible bronchoscopy demonstrated tracheomalacia and a collapse of the trachea at the stenotic segment (figure 1). The patient was scheduled for a diagnostic rigid bronchoscopy and primary resection and repair of the tracheal stenosis.
Intraoperatively, the rigid bronchoscopy revealed the presence of a 2.5-cm proximal stenotic segment (figure 2). The patient underwent orotracheal intubation, neck exploration, and tracheal dissection. The stenotic segment was isolated. It involved the distal cricoid cricoid /cri·coid/ (kri´koid)
2. the cricoid cartilage.
2. icoid cartilage and proximal trachea (Grillo type II). The stenotic segment was circumferentially mobilized and resected. The orotracheal tube was replaced with a tracheal tube through the neck incision. The posterior wall was reconstructed with interrupted Vicryl sutures (figure 3). After the posterior wall was repaired, the patient was reintubated orotracheally and the anterior wall was repaired with interrupted Vicryl sutures. The suture line was reinforced with fibrin glue laced with gentamicin gentamicin /gen·ta·mi·cin/ (jen?tah-mi´sin) an aminoglycoside antibiotic complex isolated from bacteria of the genus Micromonospora, (figure 4). The strap muscles were closed at the midline, and the skin was closed after the subcutaneous space was drained. A neck flexion stitch was applied, and the patient was extubated.
Postoperative bronchoscopy Bronchoscopy Definition
Bronchoscopy is a procedure in which a cylindrical fiberoptic scope is inserted into the airways. This scope contains a viewing device that allows the visual examination of the lower airways. showed a good airway with no granulation tissue. Ten months postoperatively, the patient still maintained a good airway (figure 5).
Tracheal resection and anastomosis is a technically difficult operation that carries the risk of life-threatening complications, including suture-line dehiscence, innominate artery erosion, and wound infection. [1-6] Grillo et al reported a large series in which the incidence of tracheal wound dehiscence or tracheal stenosis was 2.2% for tracheotracheal anastomoses, 6.1% for cricotracheal anastomoses, and 8.0% for thyrotracheal anastomoses.  They also reported that innominate artery erosion occurred in five of their 503 patients (1.0%), three of whom died (0.6%), and that major wound infections occurred in 15 patients (3.0%).
Reinforcing the suture line with fibrin glue adds strength to the line, creates an airtight seal, isolates the innominate artery from the anastomosis, and provides a medium for the local delivery of antibiotics.
Fibrin sealant (Tisseel; Baxter Healthcare Corp.; Glendale, Calif.) contains fibrinogen Fibrinogen
The major clot-forming substrate in the blood plasma of vertebrates. Though fibrinogen represents a small fraction of plasma proteins (normal human plasma has a fibrinogen content of 2–4 mg/ml of a total of 70 mg protein/ml), its conversion (a sealer sealer,
n a substance used to fill the space around silver or gutta-percha points in a pulp canal. Most contain some combination of zinc, barium, and bismuth salts and eugenol, Canadian balsam, and eucalyptol. protein) as its main active ingredient. It also contains thrombin thrombin: see blood clotting. , calcium chloride, and a fibrinolysis fibrinolysis /fi·bri·nol·y·sis/ (fi?brin-ol´i-sis) dissolution of fibrin by enzymatic action.fibrinolyt´ic
n. pl. inhibitor (aprotinin aprotinin /apro·ti·nin/ (ap?ro-ti´nin) an inhibitor of proteolytic enzymes used to reduce perioperative blood loss in patients undergoing cardiopulmonary bypass during coronary artery bypass graft. ). The two reconstituted components--the sealer protein and the thrombin solutions--are mixed and applied topically. Mixing the sealer protein and the thrombin solutions produces a viscous solution that forms an elastic coagulum coagulum /co·ag·u·lum/ (ko-ag´u-lum) pl. coa´gula [L.] clot (1).
n. pl. co·ag·u·la
1. A clot; a curd.
2. when it sets. Thrombin is a highly specific protease that transforms the fibrinogen contained in the sealer protein concentrate into fibrin. The thrombin is partly adsorbed by the fibrin that is so formed. Excess thrombin, if any, is inactivated inactivated
rendered inactive; the activity is destroyed.
treated so that they are no longer able to produce evidence of growth or damaging effect on tissue. by protease inhibitors in the blood.
Takahashi et al used tissue glue and fascia lata to reinforce tracheal anastomosis suture lines in dogs.  They found that this procedure prevented air from leaking from the suture line and decreased morbidity. Fibrin glue was also used by Gutierrez et alto repair persistent or recurrent tracheoesophageal tracheoesophageal /tra·cheo·esoph·a·ge·al/ (tra?ke-o-e-sof?ah-je´al) pertaining to the trachea and esophagus.
Of or relating to the trachea and the esophagus. fistulae.  Seguin et al used fibrin glue to repair type A aortic dissections in humans with good results.  Detweiler et al achieved normal small bowel and colon anastomoses in animals with fibrin glue and an absorbable endoluminal stent. 
Some potential complications of fibrin glue include a patient's hypersensitivity to its various components, the introduction of bacteria at the surgical site by the fibrin coagulum, and the transmission of blood-borne viruses. The risk of bacterial invasion is reduced by the addition of antibiotics to the glue.  The risk of viral transmission is minimized during the Tisseel manufacturing process, which incorporates a two-step vapor-heating process that inactivates viruses.
In conclusion, given the potential devastating complications of suture dehiscence and wound infection and the apparent safety of fibrin glue, we believe that fibrin glue appears to be a worthwhile method of reinforcing a tracheal anastomosis line. It reduces the risk of leaks, anastomosis dehiscence, innominate innominate /in·nom·i·nate/ (i-nom´i-nat) nameless.
1. Having no name.
2. Anonymous. erosion, and wound infection. Even so, a large prospective study is needed to confirm its efficacy.
(1.) Grillo HC, Donahue DM, Mathisen DJ, et al. Postintubation tracheal stenosis. Treatment and results. J Thorac Cardiovasc Surg 1995; 109:486-92.
(2.) Laccourreye O, Naudo P, Brasnu D, et al. Tracheal resection with end-to-end anastomosis for isolated postintubation cervical trachea stenosis: Long-term results. Ann Otol Rhinol Laryngol 1996; 105:944-8.
(3.) Wood DE, Vallieres E. Tracheobronchial tracheobronchial /tra·cheo·bron·chi·al/ (-brong´ke-al) pertaining to the trachea and bronchi.
Of or relating to the trachea and the bronchi. resection and reconstruction. Arch Surg 1997; 132:850-4.
(4.) Grillo HC, Donahue DM. Postintubation tracheal stenosis. Chest Surg Clin N Am 1996; 6:725-31.
(5.) Adelsmayr E, Keller C, Erd G, Brimacombe J. The laryngeal mask and high-frequency jet ventilation for resection of high tracheal stenosis. Anesth Analg 1998; 86:907-8.
(6.) Tsubota N, Yoshimura M, Miyamoto Y, et al. Primary anastomosis of the trachea: Management and pitfalls. Surg Today 1998; 28:492-7.
(7.) Takahashi N, Ichimiya Y, Mawatari T, et al. The reinforcement of tracheoplasty with a self-fascia lata and Gelatin-Resorcin-Formal (GRF) glue. Surg Today 1997; 27:1046-50.
(8.) Gutierrez C, Barrios JE, Lluna J, et al. Recurrent tracheoesophageal fistula treated with fibrin glue. J Pediatr Surg 1994; 29:1567-9.
(9.) Seguin JR, Picard E, Frapier JM, Chaptal PA. Repair of the aortic arch with fibrin glue in type A aortic dissection. J Card Surg 1994; 9:734-8.
(10.) Detweiler MB, Durastante V, Verbo A, et al. Sutureless anastomosis of the small intestine and the colon in pigs using an absorbable intraluminal stent and fibrin glue. J Invest Surg 1995; 8:129-40.
(11.) Thompson DF, Davis TW. The addition of antibiotics to fibrin glue. South Med J 1997; 90:681-4.