Real-time PCR PCR polymerase chain reaction.
polymerase chain reaction
Polymerase chain reaction (PCR) .
Ed. by M. Tevfik Dorak.
Taylor & Francis
Real-time polymerase chain reaction In Molecular Biology, real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction shifts emphasis from the end product of the biomedical bi·o·med·i·cal
1. Of or relating to biomedicine.
2. Of, relating to, or involving biological, medical, and physical sciences. technique to the whole course of the PCR process and has established itself, according to editor Dorak (School of Clinical Medical Sciences-Child Health, Newcastle U., UK), as the "most sensitive and specific quantitative PCR method." He presents a laboratory "how-to" guide to real-time PCR, first presenting chapters on the basics of data analysis, quantification, normalization, and principles of primer and probe design. This first section of the book concludes with chapters on applied aspects such as high-resolution melting analysis. The second half of the text covers applications in more detail and offers chapters on quantitative analyses of DNA methylation, mitochondrial DNA analysis, real-time immuno-PCR, clinical microbiology and virology virology, study of viruses and their role in disease. Many viruses, such as animal RNA viruses and viruses that infect bacteria, or bacteriophages, have become useful laboratory tools in genetic studies and in work on the cellular metabolic control of gene expression , solid organ transplant solid organ transplant Immunology A transplanted solid organ–eg, heart, liver, kidney, as contrasted to 'liquid' transplanted tissues–eg, BM, pancreatic islets. See Transplant, Transplantation. modeling,real-time-PCR applications in hematology, and prenatal diagnosis of monogenic disease caused by single nucleotide changes.
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