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Rapid, simple, and sensitive immunoagglutination assay with Si[O.sub.2] particles and quartz crystal microbalance for quantifying Schistosoma japonicum antibodies.

Resurgence of schistosomiasis schistosomiasis (shĭs`təsōmī`əsĭs), bilharziasis, or snail fever, parasitic disease caused by blood flukes, trematode worms of the genus Schistosoma.  has led to intensified interest in the development of methods for diagnosis, control, and prevention of this formidable disease, which is caused by parasitic Schistosoma worms [e.g., Schistosoma japonicum (Sj)] and occurs primarily in Asia, Africa, and Latin America (1). Highly sensitive RIA and enzyme immunoassay methods are commonly used, but these methods have intrinsic limitations, including time-consuming procedures, potentially dangerous materials, and expensive instruments. Therefore, there is an urgent need for rapid, simple, and inexpensive alternatives, particularly for on-the-spot epidemic screening of schistosomiasis.

Latex agglutination agglutination, in biochemistry
agglutination, in biochemistry: see immunity.
agglutination, in linguistics
agglutination, in linguistics: see inflection.
 tests (LATs) [3] first established in 1959 for the assay of serologic rheumatoid factor (2), commonly use latex microspheres with conjugated proteins to magnify effects of antigen-antibody interactions. These tests are desirably portable, fast, and simple to use, and have been used for medical diagnostic tests for more than 100 analytes (3). Most LAT assays, however, can be used only for qualitatively positive or negative decisions.

To improve the quantifying detection limits of LATs or to extend their concentration range, various analytical instruments have been introduced (4-6), but most of these measurement instruments are large and inconvenient for use in point-of-care diagnostic applications (i.e., field, ambulance, or bedside). In 1990, Kurosawa et al. (7) initially proposed the latex piezoelectric The property of certain crystals that causes them to produce voltage when a mechanical pressure is applied to them such as sound vibrations. This technique is used to build crystal microphones, phonograph cartridges and strain gauges, all of which turn mechanical movement into voltage.  immunoassay (LPEIA), which combines quartz crystal microbalance (QCM QCM Quality Control Manager
QCM Quartz Crystal Microbalance
QCM Questionnaire à Choix Multiples
QCM Quebec Cartier Mining
QCM Quark Content Management
QCM Queen City Metro (Cincinnati bus) 
) measurements with an LAT immunoassay. The selective agglutination of immunosensitized latex microspheres in the presence of the opposite analytes leads to frequency changes recognized by the QCM monitor (7-9). The LPEIA offers a portable, sensitive, cost-effective method that does not require immobilization Immobilization Definition

Immobilization refers to the process of holding a joint or bone in place with a splint, cast, or brace. This is done to prevent an injured area from moving while it heals.
 of immunoactive agents. Unfortunately, this method has been limited to large-scale use, mainly because of undesirable physicochemical properties of latex materials, including large size, poor stability, low protein-loading ability, and nonspecific aggregation from solution backgrounds.

In recent years, the rapid development of materials with many improved properties for bioanalytical applications (3,10) has led to a resurgence of interest in the development of particle-enhanced immunoassays. Manometer- or submicrometer-sized particles may be suitable for designing innovative agglutination-based diagnostic tests (3,10-13). For example, Costanzo et al. used biotinmodified Si[O.sub.2] nanoparticles for the agglutination-based analysis of avidin av·i·din
n.
A protein, found in uncooked egg white, that binds to and inactivates biotin and which, when present in abundance, can result in a deficiency of biotin.
 with a complex, dynamic light-scattering instrument, enabling investigation of aggregation rates for various-sized nanoparticle mixtures (14). Because silica (Si[O.sub.2]) particles have higher density (~2 g/mL) (3,10,15), greater surface-to-volume ratio, and more rugged surfaces than do polymeric latex beads, small Si[O.sub.2] particles may facilitate larger anchoring of immunoactive entities. Moreover, [Si.sub.2] particles with hydroxyls on their surfaces are more hydrophilic hydrophilic /hy·dro·phil·ic/ (-fil´ik) readily absorbing moisture; hygroscopic; having strongly polar groups that readily interact with water.

hy·dro·phil·ic
adj.
 and less susceptible to physical deformation than polymeric latex microspheres and thus are less likely to produce hydrophobic interactions, a potential cause for aggregation or instability. In addition, chemically functionalized Si[O.sub.2] particles may provide a sol-gel matrix for firm and efficient binding of Sj antigens (SjAg) from adult worms or eggs, which are labile labile /la·bile/ (la´bil)
1. gliding; moving from point to point over the surface; unstable; fluctuating.

2. chemically unstable.


la·bile
adj.
1.
, large-sized, and otherwise difficult to immobilize im·mo·bi·lize
v.
1. To render immobile.

2. To fix the position of a joint or fractured limb, as with a splint or cast.



im·mo
. In the present work, we used submicrometer-sized Si[O.sub.2] particles, sensitized with SjAg, as replacements for latex microspheres in a quantitative LPEIA alternative method for measuring Sj antibodies (SjAb) in infected rabbit serum (IRS An abbreviation for the Internal Revenue Service, a federal agency charged with the responsibility of administering and enforcing internal revenue laws. ) and human serum specimens.

Materials and Methods

APPARATUS

The transmission electron microscope (TEM TEM

1. transmission electron microscope.

2. triethylenemelamine.

3. transmissible encephalopathy of mink.
; Hitachi-H800) was from Hitachi, Ltd. Copper grids (No. 50-230) were provided by Scientific Instruments Ltd. of the Chinese Academy of Science (Beijing, China). The quartz crystal analyzer was obtained from SEIKOEG&G Co. Ltd., and QCMs (9 MHz, gold electrodes), which we operated at their fundamental frequencies, were from Chenxing Radio Equipment. One side of the QCM was sealed with an O-ring of silicone rubber. The ultrasonic rinse implement (B220S-T) was from Binengxin Ultrasonic, Ltd. The platform-type centrifuge was from Pingfan Instruments, Ltd. A laboratory-made detection cell was used for the agglutination-based immunoassay, in which an optimized assay buffer solution was gently agitated with a magnetic stirrer. The experimental temperature was controlled at 25[degrees]C with a thermostat obtained from Chongqing Experimental Equipment (Model CSS (1) See Cascading Style Sheets.

(2) (Content Scrambling System) The copy protection system applied to DVDs, which uses a 40-bit key to encrypt the movie.
 501).

REAGENTS

We purchased 3-(Aminopropyl) trimethoxysilane from Sigma-Aldrich; tetraethyl orthosilicate (TEOS TEOS Tetraethylorthosilicate
TEOS Tetra Ethyl Oxysilane
TEOS Trusted E-Mail Open Standard
) from Wulian Chemicals; ammonia, ethanol, methanol, and polyethylene glycol) (PEG, [M.sub.r] 6 000) from Tiantai Fine Chemistry and Engineering Company; and glutaraldehyde glutaraldehyde /glu·ta·ral·de·hyde/ (gloo?tah-ral´de-hid) a disinfectant used in aqueous solution for sterilization of non-heat–resistant equipment; also used as a tissue fixative for light and electron microscopy.  (GLU (language) GLU - A practical coarse grain implementation of the Lucid dataflow language for networks. ) from Wuhan Organic Chemistry Reagent Company. SjAg (4.2 g/L, [M.sub.r] 32 000) were obtained from soluble homogenate homogenate /ho·mog·e·nate/ (ho-moj´in-at) material obtained by homogenization.

homogenate

material obtained by homogenization.
 of adult Sj worms through an elaborated purification procedure as described in (16). Sj antibodies [(SjAb) 2.1 g/L] were obtained by purifying the immunoglobulins of IRS. Normal rabbit serum (MRS MRS - Modifiable Representation System.

An integration of logic programming into Lisp.

["A Modifiable Representation System", M. Genesereth et al, HPP 80-22, CS Dept Stanford U 1980].
), IRS, and human serum specimens were kindly provided by the Institute of Schistosomiasis, Xiangya School of Medicine, Central South University (Hunan, China). Bovine serum albumin (BSA 1. BSA - Business Software Alliance.
2. BSA - Bidouilleurs Sans Argent.
) was purchased from Shanghai Shensuo Biological Products. Phosphate buffered saline Phosphate buffer saline (abbreviated PBS) is a buffer solution commonly used in biochemistry. It is a salty solution containing sodium chloride, sodium phosphate and potassium phosphate. The buffer helps to maintain a constant pH.  (PBS PBS
 in full Public Broadcasting Service

Private, nonprofit U.S. corporation of public television stations. PBS provides its member stations, which are supported by public funds and private contributions rather than by commercials, with educational, cultural,
) solutions of various pH were prepared with 0.01 mol/L [Na.sub.2]HP[O.sub.4] and 0.01 mol/L [KH.sub.2]P[O.sub.4.] The Piranha reagent was a mixture of [H.sub.2][O.sub.2] and [H.sub.2]S[O.sub.4] at a volume ratio of 3:7. All other reagents were of analytical reagent grade. Doubly distilled water was used throughout the experiments.

PREPARATION AND IMMUNOSENSITIZATION OF SILICA PARTICLES

Synthesis and surface functionalization of Si[O.sub.2] particles. We prepared Si[O.sub.2] particles with the tetraethyl orthosilicate hydrolyzation procedure with ammonia as the catalyzer and PEG as the surfactant Surfactant Definition

Surfactant is a complex naturally occurring substance made of six lipids (fats) and four proteins that is produced in the lungs. It can also be manufactured synthetically.
, according to the method reported elsewhere (17). The submicrometer-sized silica particles were ultrasonically washed in methanol and water, each for 10 min, and were sequentially centrifuged at ~112.48 force to eliminate any unreacted chemical substances.

A portion of the dried Si[O.sub.2] particles (~1.0 g) was ultrasonically resuspended in the mixed solution of methanol (10 mL) and 3-(aminopropyl) trimethoxysilane (0.50 mL) and stirred overnight at room temperature so as to complete the silanized reaction. Then the mixture was isolated by centrifuge and washed several times with methanol and PBS solution (pH 7.0). The resulting amine-functionalized Si[O.sub.2] particles were stored at 4 [degrees]C in PBS solution (pH 7.0, 4.0 mL) containing 1.5% PEG.

Preparation of SjAg-derivatized Si[O.sub.2] particles. The prepared amine-functionalized Si[O.sub.2] particle suspension (10 mL) was ultrasonically resuspended in 2.5% GLU (5.0 mL), stirred for 3 h at room temperature to achieve the aldehyde-activated surface, and then washed twice with water. The resulting silica particles were suspended in the PBS solution (pH 7.0, 4.0 mL), followed by the addition of a portion of SjAg solution (4.2 g/L,1.0 mL) with continual stirring for 3 h at room temperature. After being washed twice with water, the obtained SjAg-derivatized Si[O.sub.2] particles were resuspended in PBS solution (pH 7.0, 4.0 mL). We then introduced an appropriate BSA-NRS reagent (consisting of 10 g/L BSA and NRS at a 1:1 volume ratio) into the silica suspension, which was then stirred for 1.5 h to block the unreacted aldehyde aldehyde (ăl`dəhīd) [alcohol + New Lat. dehydrogenatus=dehydrogenated], any of a class of organic compounds that contain the carbonyl group, and in which the carbonyl group is bonded to at least one hydrogen; the general  and any nonspecific adsorption sites on the silica surfaces. Furthermore, we isolated the centrifuged SjAg-derivatized silica suspension and washed it twice with PBS solution (pH 7.0). Finally, the resulting immunosensitized Si[O.sub.2] particles were stored in PBS solution (pH 7.0, 5.0 mL) containing 1.5% PEG at 4 [degrees]C. This preparation may remain stable, with no significant change in immunoactivity, for ~3 months, but the silica particle suspensions should be ultrasonically resuspended before use.

TEM MEASUREMENTS

We dropped 15 [micro]L of the immunoagglutination assay solution with SjAg-derivatized Si[O.sub.2] particles of ~1.76 x [10.sup.15] beads/mL in the presence of 21.0 mg/L SjAb onto a copper grid. Another portion of SjAg-sensitized silica particle suspension without added SjAb was processed in parallel as the control test. Each of the grids was then drained on tissue paper and air-dried. We then carried out separate high-resolution TEM measurements (images shown in Fig. 1).

IMMUNOAGGLUTINATION ASSAY PROCEDURE

Before analysis, the clean QCM probe was incubated with a portion of BSA solution (10 g/L, 20 [micro]L) for 1 h at 37[degrees]C to block any protein-nonspecific adsorption sites on the crystal. The BSA-modified QCM probe was then inserted into the detection cell with 3.0 mL of the optimized assay buffer solution (pH 7.0, containing 1.5 g/L PEG and 40 mmol/L NaCI). A schematic diagram of the total QCM system setup is shown in Fig. 2. Under gentle stirring and thermostatic control, 80 [micro]L SjAg-derivatized Si[O.sub.2] particle suspension was introduced with the microinjector into the assay solution. After stabilization of the resonance frequency (shift <~1.0 Hz/min) within several minutes, 25 [micro]L of each serum sample to be analyzed was separately injected into the detection cell. The induced frequency shifts were monitored in real time by the quartz crystal analyzer, and the data were displayed and recorded by the linking computer. To avoid possible errors from different additions of analytes and any nonspecific background adsorption, we recorded the frequency responses to the analytes from 30 s after analyte addition until equilibrium was reached. For all experiments, unless otherwise indicated, the recorded frequency responses were the mean (SD) frequency changes of triplicate measurements.

At the completion of each detection assay, the assay buffer solution and the silica immunoagglutinated entities were discharged from the detection cell, which we then rinsed twice with PBS. Moreover, QCM probes used repeatedly for ~3-5 immunoassay runs were treated with Piranha reagent for ~5 min to peel off any proteins from the crystal, then rinsed twice with water and air-dried. The regenerated QCM probes were remodified with BSA for the next assay cycle.

Results and Discussion

IMMUNOAGGLUTINATION QCM ASSAY WITH SILICA PARTICLES

QCM devices are highly sensitive to changes of the surface mass loading of adsorbates and the interfacial properties relating to some nonmass response factors of the test solution, such as the density ([rho]), viscosity ([eta]), and conductivity (18-22). We used submicrometer-sized Si[O.sub.2] particles as replacements for traditional latex microspheres to label SjAg for a new QCM immunoagglutination assay. The specific agglutination of SjAg-derivatized Si[O.sub.2] particles in the presence of SjAb targets induced a primarily linear change of [([rho][eta]).sup.1/2] leading to proportional frequency shifts of the crystal, which can be derived from the pioneering theory model (equation) for a QCM in contact with liquid (21-22). The measured frequency changes should be linearly related to SjAb concentrations in samples to be quantified. The silica immunoagglutination events in the presence and absence of SjAb were comparably characterized by the high-resolution TEM (Fig. 1). Before the immunoagglutination, silica particles in the suspension are mostly spherical, uniform, and monodispersed, with an estimated mean diameter of ~280 nm (Fig.1A). When the immunoagglutination occurred in the presence of SjAb, the TEM image fields were dominated by large micrometer-sized aggregations (Fig. 1B), which may be the source of the changes in the solution parameters (i.e., [rho] and [eta]) that induced the frequency shifts of the QCM probe.

The submicrometer-sized silica particles used in these experiments have properties that may make them more suitable for immunoagglutination assays than are common polymer latex (i.e., polystyrene) microspheres. These special properties include larger surface-to-volume ratio, higher density, more hydrophilic surface, and higher colloidal colloidal

of the nature of a colloid.


colloidal bath
a bath containing gelatin, bran, starch or similar substances, to relieve skin irritation and pruritus.
 stability. Moreover, silica particles derivatized with amine groups by silanization might provide an efficient sol-gel matrix for increased binding of SjAg through entrapment entrapment, in law, the instigation of a crime in the attempt to obtain cause for a criminal prosecution. Situations in which a government operative merely provides the occasion for the commission of a criminal act (e.g.  and GLU-mediated covalence covalence /co·va·lence/ (ko-va´lens)
1. the number of electron pairs an atom can share with other atoms.

2. one or more chemical bonds formed by sharing of electron pairs between atoms.
 interactions. Therefore, a silica particle-enhanced immunoagglutination assay with the highly sensitive QCM measurements might be expected.

DOSAGE OF IMMUNOSENSITIZED SILICA PARTICLES

We used SjAg-derivatized silica suspensions with various particle concentrations to investigate the effects of immunosensitized Si[O.sub.2] particle dosage on silica immunoagglutination assays for SjAb serum samples (Fig. 3). The frequency responses obtained for the immunoagglutination assays peaked at ~1.76 x [10.sup.15] beads/mL of the silica particles (Fig. 3). Particle concentrations that are too high or too low may lead to a serious imbalance of antigenantibody ratios and thus decrease formation of lattice-like immunocomplexes (23), resulting in very little ([rho][eta]).sup.1/2] change of the assay solution or, conversely, steric steric /ste·ric/ (ster´ik) pertaining to the arrangement of atoms in space; pertaining to stereochemistry.

ster·ic or ster·i·cal
n.
 hindrance of silica agglutination that is too high, as manifested by frequency responses.

OPTIMIZATION OF IMMUNOAGGLUTINATION ASSAY MEDIUM COMPOSITION

Because composition of the assay buffer may play a vital role in controlling the physicochemical processes of immunological recognition and agglutination (13,24-25), we sought to optimize the composition of the reaction medium for the immunoagglutination assays within required ranges of pH, ionic strength, and surfactants.

We investigated the effects of pH values on the frequency responses of silica immunoagglutination (see Fig. 1 in the Data Supplement that accompanies the online version of this article at http://www.clinchem.org/ content/vol52/issuell). The biggest frequency response was obtained with the assay solution at pH 7.0. Such a neutral condition might optimize the recognition of SjAb by SjAg-sensitized silica particles and subsequently the agglutination by appropriate electrostatic forces, because the immunoagglutination interaction process might involve pH-dependent charged protein species. Moreover, it was found that the frequency responses tend to slightly increase when pH <2.0, presumably pre·sum·a·ble  
adj.
That can be presumed or taken for granted; reasonable as a supposition: presumable causes of the disaster.
 because of the nonspecific acid agglutination of silica particles.

[FIGURE 3 OMITTED]

We investigated the optimum ionic strength for the silica immunoagglutination assays by adding various concentrations of NaCl to the assay buffer solution (see Fig 2 in the online Data Supplement). We observed that the frequency responses to immunoagglutination increased with the increase in NaCl concentrations, up to 40 mmol/L. When the concentrations of added salt were increased to an ionic strength >40 mmol/L NaCl, a gradual decrease in the frequency response occurred. Moreover, suitable increases in NaCl concentrations experimentally increased the immunoagglutination response rates (data not shown), suggesting that salt in the test solution might in some way promote the immunorecognition/agglutination of Si[O.sub.2] particles. However, excessive ionic strength in the test solution (>40 mmol/L NaCl, the optimum ionic strength in test solution) may hamper silica immunoagglutination, inducing nonspecific precipitation of labile substances in the samples.

PEG as a polyelectrolyte pol·y·e·lec·tro·lyte
n.
An electrolyte, such as a protein or polysaccharide, having a high molecular weight.
 surfactant has been commonly used as the accelerator or precipitant of immunoagglutination for various homogeneous immunoassays. We studied the promotion effects of PEG on the silica immunoagglutination by adding PEG to the assay buffer solution and compared frequency responses between the immunoagglutination assays with and without PEG (see Fig 3 in the online Data Supplement).Use of PEG could obviously speed the immunoagglutination to equilibrium and amplify the frequency response signal, shifting from ~332 Hz to ~565 Hz, possibly because the steric exclusion contributed by PEG could accelerate silica immunoagglutination in the presence of the complementary targets (13, 25).

DEPRESSION OF NONSPECIFIC SILICA IMMUNOAGGLUTINATION

Nonspecific silica agglutination adversely affects analytical performances of the immunoagglutination QCM assays through the nonspecific surface adsorption and selfaggregation of silica particles. By use of BSA-NRS reagent to treat SjAg-derivatized Si[O.sub.2] particles, we blocked blank sites on silica particle surfaces that might conduct nonspecific adsorptions. We observed a difference in frequency change of ~100 Hz for SjAg-derivatized silica particles treated with BSA-NRS compared with the untreated particles (see Fig. 4). The silica particles were disposed of or stored in PBS solution containing 1.5% PEG, in which PEG chains may be entangled on the anchored silica particle surface, forming steric and electrostatic barriers to the self-aggregation of these particles, even at high ionic strength, as observed elsewhere for the polymer microspheres (26-27). Additionally, premodification of the QCM probe with BSA could greatly reduce or eliminate adventitious ADVENTITIOUS, adventitius. From advenio; what comes incidentally; us adventitia bona, goods that, fall to a man otherwise than by inheritance; or adventitia dos, a dowry or portion given by some other friend beside the parent.  nonspecific adsorption on the crystals of proteins or molecules from complex sample backgrounds.

[FIGURE 4 OMITTED]

CHARACTERISTICS OF FREQUENCY RESPONSE FOR IMMUNOAGGLUTINATION

We investigated characteristics of time-dependent frequency response for silica immunoagglutinations under optimized assay conditions, in which the immunoaggregation processes were monitored in real time by the QCM system (Fig. 4). The observed frequency shift trends were quite different for IRS vs NRS as the control test. The response values obtained for the former (Fig. 4B, ~567 Hz) were 3-fold greater than those for the latter (Fig. 4A, ~121 Hz), indicating that the frequency response to the nonspecific agglutination of NRS (the blank or noise signal) may be insignificant. These observations may demonstrate that the silica immunoagglutination is attributable only to specific SjAg-SjAb interactions. The total time for the specific silica immunoagglutination process was ~35 min, which may vary with the target SjAb concentrations in the samples.

QUANTITATIVE ANALYSIS

We investigated the quantitative analysis capabilities of the developed immunoassay under optimized conditions with a series of serum samples with various SjAb concentrations. The calibration curve describing the relationship between the frequency changes ([DELTA]F) of immunoagglutination and SjAb concentrations is shown in Fig. 5. The [DELTA]F values were highly linear for SjAb concentrations of ~0.70-32.31 mg/L. The detection limit was estimated to be ~0.46 mg/L according to the 3 SD rule, defined as the concentration at the mean (3 SD) of several determinations (here, 6 determinations) of the blank or zero calibration (28). The response signals may saturate sat·u·rate
v. Abbr. sat.
1. To imbue or impregnate thoroughly.

2. To soak, fill, or load to capacity.

3. To cause a substance to unite with the greatest possible amount of another substance.
 at >32.31 mg/L SjAb, presumably because of the limited amount of SjAg available on the surfaces of silica particles; moreover, a value of 21.0 mg/L SjAb serum was assayed 5 times. The obtained mean (SD) frequency change was 563 (57) Hz, with a relative SD of 10.3%, reflecting good analytical reproducibility of the proposed technique.

[FIGURE 5 OMITTED]

SPECIMEN EVALUATION

The developed QCM immunoaggglutination system was applied to evaluate several unpurified Adj. 1. unpurified - not made pure
impure - combined with extraneous elements
 IRS specimens with various degrees of Sj infection, denoted by the numbers of Sj cercarias (parasitic larvae) and days of infection. The results of frequency shifts were recorded in real time (see Fig 4 in the online Data Supplement). We observed that frequency responses increased with an increase in the degree of Sj infection of the samples. For example, IRS specimens infected by 1000 Sj cercarias for 31, 45, and 54 days corresponded to mean (SD) frequency changes of 312 (34) Hz, 449 (38) Hz, and 506 (42) Hz, respectively. Meanwhile, the frequency changes obtained for the IRS samples infected for 54 days with varying Sj cercarias numbers of 1000, 2000, and 3500 were 506 (42) Hz, 622 (59) Hz, and 815 (67) Hz, respectively. Accordingly, the developed immunoagglutination assay can easily define the Sj infection degree of IRS specimens. Of note, positive samples from patients may be identified by frequency response values >3 times those of the blank in the control test.

To further investigate the feasibility of the developed QCM immunoassay for practical applications, we analyzed 8 human serum specimens obtained from patients living in endemic schistosomiasis areas, and compared our SjAb titer results with those obtained by ELISA ELISA (e-li´sah) Enzyme-Linked Immuno-Sorbent Assay; any enzyme immunoassay using an enzyme-labeled immunoreactant and an immunosorbent.

ELISA
n.
 performed at the Institute of Schistosomiasis, Central South University (Fig. 6). By comparing the results, we obtained a regression equation of y = 1.009 x + 61.68, with a correlation coefficient of 0.9827. Moreover, the statistically significant difference estimated by the common F-test showed no significant difference (P > 0.05) between the results of the 2 methods. In particular, as can be seen from Fig. 6, they showed consistent results in identifying specimens with very low or zero SjAb titers. These results indicate that the QCM immunoassay should be comparable to ELISA in terms of analytical sensitivity and specificity, and this provides an alternative tool for rapid clinical diagnosis, particularly for epidemic screening of schistosomiasis.

[FIGURE 6 OMITTED]

Conclusion

We have successfully extended LAT technology to develop a silica particle-enhanced immunoagglutination assay for quantifying SjAb through sensitive QCM measurements. The proposed assay enables quantitative detection of SjAb concentrations as low as ~0.46 mg/L. Specimens with different degrees of Sj infection can be easily identified with no sample pretreatment pretreatment,
n the protocols required before beginning therapy, usually of a diagnostic nature; before treatment.

pretreatment estimate,
n See predetermination.
. Our results for human serum specimens demonstrate that the detection sensitivity and specificity of the QCM immunoassay are comparable to those of ELISA. In addition, our portable system offers advantages over the conventional method in that the analytical operations are more rapid, easier to perform, and less expensive. The new QCM method can be tailored as a bedside or field-applicable tool for rapid clinical diagnosis and screening of schistosomiasis in epidemic situations, and can be further integrated and miniaturized as a commercial chip. Moreover, this novel technology might be extended to other immunoagglutination tests for wider diagnostic applications.

This work was supported by the National Natural Science Foundation of China (Nos. 20435010 and 20375012), and the Science Commission of Hunan Province.

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HUA WANG, [1] * YUN ZHANG, [1] BANI YAN, [1] LI LIU, [1] SHIPING WANG, [2] GUOLI SHEN, [1] * and RuQIN YU [1]

[1] State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P.R. China.

[2] Institute of Schistosomiasis, Xiangya School of Medicine, Central South University, Changsha,Chma.

[3] Nonstandard abbreviations: LAT, latex agglutination tests; LPEIA, latex piezoelectric immunoassay; QCM, quartz crystal microbalance; S'OZ, silica; SjAg, Schistosoma japonicum (Sj) antigens; SjAb, Sj antibodies; IRS, infected rabbit serum; TEM, transmission electron microscope; PEG, poly(ethylene glycol); GLU, glutaraldehyde; NRS, normal rabbit serum; BSA, bovine serum albumin; PBS, phosphate-buffered saline.

* Address correspondence to these authors at: State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China. Fax 86-7318821355; e-mail huawang@hnu.cn, glshen@hnu.cn.

Received April 8, 2006; accepted August 16, 2006.

Previously published online at DOI: 10.1373/clinchem.2006.071555
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Title Annotation:Automation and Analytical Techniques
Author:Wang, Hua; Zhang, Yun; Yan, Bani; Liu, Li; Wang, Shiping; Shen, Guoli; Yu, Ruqin
Publication:Clinical Chemistry
Date:Nov 1, 2006
Words:4306
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