Rad-4. Development of a novel image guided technique for the assessment of osteogenesis using a rat model with induced bone trauma in pre-clinical model.Evaluation of osteogenesis osteogenesis /os·teo·gen·e·sis/ (os?te-o-jen´e-sis) the formation of bone; the development of the bones.osteogenet´ic osteogenesis imperfec´ta following surgically induced and immobilized fractures in laboratory animals over a period of time in a sequential fashion often involves enrollment of a large number of animals. A certain number of animals have to be periodically sacrificed to adequately assess the progress of the experiment. The goal of this project was to explore the feasibility of using imaging technique as a tool to serially evaluate the induced femoral fractures in a rat model. Adult male rats (n = 50) were divided into experimental (5 mm femoral segmental defect [FSD], and FSD plus Delivery Capsules) and control groups. All surgical procedures were conducted following IACAUC approved protocol. Live animals were subjected to imaging setting following anesthesia (Xylazine, 10 mg/kg; Ketamine ketamine /keta·mine/ (ke´tah-men) a rapid-acting general anesthetic, used as the hydrochloride salt. ke·ta·mine n. , 75 mg/kg). Digitized data were generated by rotating head angiographic unit as well as a multi-slice CT scanner with helical method and processed in a dedicated computer system with algorithms to reconstruct 3-dimensional images in various angles with the capabilities of subtracting overlying overlying suffocation of piglets by the sow. The piglets may be weak from illness or malnutrition, the sow may be clumsy or ill, the pen may be inadequate in size or poorly designed so that piglets cannot escape. soft tissues or bones. The radiographic radiographic (rā´dēōgraf´ik), adj relating to the process of radiography, the finished product, or its use. findings (3-dimensional mode) were analyzed for definition of the immobilizing hardware, appearance of the fracture sites, proximity and appearance of the implanted bone-growth enhancing agent delivery capsules contiguous to the fracture sites. Results of this project suggested that (1) the use of imaging methods may offer an advantage by reducing the number of animals that have to be involved in the experiment, and (2) the use imaging methods could provide an accurate assessment of the status of the healing process (termination of the treatment vs additional treatment) without sacrificing the animals (predicting prognosis). Rishi rishi (rēˑ·shē), n in Sanskrit, one who possesses knowledge. Roy, Michelle Tucci, Ramesh Patel, Ashraf Ragab, George Russell, and Hamed Benghuzzi. University of Mississippi Medical Center University of Mississippi Medical Center (UMC) is the health sciences campus of the University of Mississippi (Ole Miss). Located in Jackson, Mississippi (USA), it houses the Schools of Medicine, Dentistry, Nursing, Health Related Professions, and Graduate Studies in the Health , Jackson MS. |
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