RNAi Research Races From The Stalls With IDT's New TriFECTa(TM) Kit.

CORALVILLE, Iowa -- Integrated DNA Technologies' Kit Uses Dicer-Substrate RNAi To Achieve Knockdown Rates Superior to Traditional 21-mer Kits.

Integrated DNA Technologies (IDT), the largest supplier of custom nucleic acids in the U.S., announces the launch of the TriFECTa(TM) Dicer-Substrate RNAi Kit. Employing 27-mer duplex RNA, TriFECTa delivers a significant design improvement over first-generation 21-mer based RNAi kits. Processed by Dicer into 21-mer siRNAs, IDT's new 27-mer RNAi design delivers up to a 100-fold increased potency when compared to older 21-mer siRNA models at the same site.

Utilizing the endogenous Dicer system, IDT's TriFECTa represents a substantial improvement over traditional antisense based processes, and gives researchers the confidence they need when studying hard-to-target genes. Backed by the companies functional knockdown guarantee, at least one of the kit's three Dicer-Substrate RNAi duplexes will deliver a 70% knockdown of the target mRNA when greater than 90% transfection efficiency, and greater than 90% knockdown with positive control is demonstrated.

"Designing RNAi duplexes that engage and exploit Dicer's excellent natural capabilities rather than avoiding it using siRNAs enables researchers to significantly raise efficacy - often at sub-nanomolar concentrations," noted Dr. Mark Behlke, vice president of molecular genetics at IDT. "Our research with City of Hope revealed that researchers can achieve dramatically improved levels of RNAi-mediated gene knockdown at significantly lower concentrations than was previously possible."

The TriFECTa kit includes:

--Three target-specific Dicer-Substrate siRNA duplexes (5 nmoles each)

--Cy3TM DS Transfection Control, fluorescent-labeled transfection control duplex (1 nmole)

--HPRT-S1 DS Positive Control duplex (1 nmole)

--DS Scrambled-Neg, universal negative control duplex (1 nmole)

--RNase Free Duplex Buffer (100mM KAc/30 mM HEPES pH 7.5)

About IDT

Integrated DNA Technologies, Inc. (IDT) is the largest supplier of custom nucleic acids in the U.S., serving the areas of academic research, biotechnology, clinical diagnostics and pharmaceutical development. IDT's primary business is the manufacturing of custom DNA and RNA oligonucleotides (oligos) for research applications.

Founded in 1987 by Joseph Walder, M.D., Ph.D. (University of Iowa), Integrated DNA Technologies, Inc. has achieved annual double-digit growth over the past 10 years. Today, IDT synthesizes and ships up to 25,000 custom oligos per day, and more than 60,000 customers worldwide turn to IDT to fulfill their synthetic DNA and RNA requirements.

All IDT's production processes for both synthetic oligonucleotides and consumables at its Coralville, Iowa, headquarters are ISO 9001:2000 certified.