Proteopure Announces New Proteomic Sample Preparation Kit.PITTSBURGH -- Proteopure, Inc. announced today that its ProteoHook(TM) 2DE Sample Preparation Kit is available for sale to Life Science researchers who want a better method for protein sample preparation. ProteoHook(TM) 2DE is optimized for use in preparing samples for 2D gel electrophoresis gel electrophoresis n. Electrophoresis performed in a gel composed of agarose, polyacrylamide, or starch. . It can be used with any sample type, eliminating the need for multiple kits. The kit uses spin columns and includes all necessary buffers both to simplify the process and to provide reliable, consistent results. Current protein sample preparation methodologies (commercial kits and home-brews) are mainly based on protein precipitation Precipitation is widely used in downstream processing of biological products, such as proteins. [1] This unit operation serves to concentrate and fractionate the target product from various contaminants. and are inherently unpredictable as contaminants often co-precipitate with proteins and precipitated proteins are often insoluble insoluble /in·sol·u·ble/ (in-sol´u-b'l) not susceptible of being dissolved. in·sol·u·ble adj. Not soluble. . Proteopure's core technology effectively addresses these issues as proteins are always maintained in solution and due to the properties of the ProteoHook(TM) no proteins are lost during the clean-up process. Analyzing the proteome pro·te·ome n. The complete set of proteins that are produced by the genes of an organism. proteome the entire complement of proteins produced by a cell. of a cell is very challenging. Each cell can express up to 10,000 different proteins that vary over a wide range of concentrations. To analyze these proteins, they must be physically separated into discrete pools using different separation technologies (two-dimensional gel electrophoresis Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels. , column chromatography column chromatography n. A form of partition chromatography in which a liquid phase flows down a column packed with a solid phase. and others). Once separated, individual proteins are identified by determining unique peptide patterns or amino acid amino acid (əmē`nō), any one of a class of simple organic compounds containing carbon, hydrogen, oxygen, nitrogen, and in certain cases sulfur. These compounds are the building blocks of proteins. sequences. It is therefore critical that during the sample preparation process no proteins are lost and all contaminants are removed for reproducible proteomic results. Proteopure develops and markets sample preparation kits for proteomic research. Proteopure's core technology uses a novel protein capture technology that specifically binds proteins while all contaminants and other unwanted cell materials are washed away. The technology eliminates precipitation-related losses and produces better, more reliable results for scientists seeking to understand cell functions and discover new biomarkers and drug targets. The Company's website is www.proteopure.com. |
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