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Processing controls in blood collection tubes reveals interference.

To the Editor:

Central tenets of quality control (QC) and quality assurance are that (a) controls should be handled by the regular staff, and (b) they should be treated the same way as an equivalent patient specimen. This is not always adhered to in routine practice, and recent problems with blood collection tubes underline this point (1-4). Collection of blood in certain batches of Vacutainer[R] SST SST: see airplane.  tubes was associated with errors in some immunoassays on certain immunoassay platforms [e.g., biases of 20% to 30% for total thyroxine ([T.sub.4]), total triiodothyronine triiodothyronine /tri·io·do·thy·ro·nine/ (tri?i-o?do-thi´ro-nen) one of the thyroid hormones, an organic iodine-containing compound liberated from thyroglobulin by hydrolysis. It has several times the biological activity of thyroxine.  ([T.sub.3]), and cortisol cortisol (kôr`tĭsôl') or hydrocortisone, steroid hormone that in humans is the major circulating hormone of the cortex, or outer layer, of the adrenal gland.  and -15% to 20% for follicle-stimulating hormone (FSH FSH follicle-stimulating hormone.

FSH
abbr.
follicle-stimulating hormone


Facioscapulohumeral muscular dystrophy (FSH) 
)]. These errors have been attributed to contamination of samples with surfactants that are used to coat the tubes; the surfactant Surfactant Definition

Surfactant is a complex naturally occurring substance made of six lipids (fats) and four proteins that is produced in the lungs. It can also be manufactured synthetically.
 can release reagent antibodies from some solid-phase materials used in immunoassay methods (5, 6).

Blood specimens for testing are removed through a metal needle into a blood collection tube. The tube and its contents are centrifuged to isolate serum, and the serum is either sampled directly from this tube or transferred to another tube from which a sample is removed for analysis. In contrast, a QC material is poured into a sample cup or tube from which it is sampled for analysis. Thus, the control material, unlike the blood specimen, does not come into contact with the surface of a metal needle or the contents of a blood collection tube.

We reasoned that adverse effects of additives in blood collection tubes would be apparent if control materials were poured into blood collection tubes and processed in the same way as blood. To test this, we analyzed control materials that were poured into our current batch of SST tubes or into SST tubes from the variant lot shown to be the source of an immunoassay interference. We did not introduce the control material through a needle because we, like other laboratories, strive to limit the use of needles. The tubes were then processed as if they were routine blood specimens, and the materials were analyzed.

We used 3 control materials: Performance Verifier 1 (lot no. L5782; Ortho Clinical Diagnostics), Lyphochek Immunoassay Plus Control (lot no. 40160), and Lyphochek Anemia Control (lot no. 43100; both from Bio-Rad Laboratories). We also used 3 types of blood collection tubes: BD Vacutainer glass tubes and BD Vacutainer plastic SST tubes (Becton Dickinson) from our routine supply, and BD Vacutainer plastic SST tubes from the variant lot of tubes (supplied by Becton Dickinson). Analytical results obtained for the chemistry and endocrine tests were compared with those obtained by analysis of the control material directly from the bottle.

For common chemistry tests (glucose, urea, creatinine, sodium, potassium, chloride, iron, carbon dioxide, amylase amylase (ăm`əlās'), enzyme having physiological, commercial, and historical significance, also called diastase. It is found in both plants and animals. Amylase was purified (1835) from malt by Anselme Payen and Jean Persoz. , lipase lipase (lī`pās), any enzyme capable of degrading lipid molecules. The bulk of dietary lipids are a class called triacylglycerols and are attacked by lipases to yield simple fatty acids and glycerol, molecules which can permeate the membranes , calcium, magnesium, phosphate, cholesterol, triglycerides, uric acid, total protein, albumin, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, creatine kinase, [gamma]-glutamyl transpeptidase, alkaline phosphatase, total bilirubin Bilirubin

The predominant orange pigment of bile. It is the major metabolic breakdown product of heme, the prosthetic group of hemoglobin in red blood cells, and other chromoproteins such as myoglobin, cytochrome, and catalase.
, conjugated bilirubin, and unconjugated bilirubin) performed on the Vitros 950AT (Ortho Clinical Diagnostics), we observed no differences between results for the control analyzed directly or after processing in the current or in the variant lot of SST tubes. Results for all replicates of the controls were within the established QC ranges for all analytes for all of the samples. This is in accord with the absence of any reported biases for common chemistry tests in tubes from affected batches.

By contrast, the endocrine tests run on an hninulite 2000 (Diagnostic Products Corporation) showed significant processing-dependent differences in the results for the analytes previously affected by surfactants in SST tubes (Table 1). Total [T.sub.4] results for all of the controls processed in SST tubes from the variant lot were above the established QC intervals in all tubes (triplicate) and at all concentrations of the controls. Several of the total [T.sub.3] and cortisol results for the QC samples were at or above the established QC interval and would be regarded with suspicion (3 of 9 tubes for [T.sub.3] and 2 of 9 tubes for cortisol). The results for FSH had the reported negative bias, and a substantial number of QC results (5 of 9 tubes) were at or outside the limits of the established QC intervals. As expected, for the controls processed in the current lot of SST tubes and for controls that had not come into contact with an SST tube, control limits were not exceeded for the overwhelming majority of results (70 of 71 results, with 1 missing value).

Other factors, such as the volume of the control material, length of incubation (~2 h) in the tube, and contact with the stopper and its lubricant, may influence the magnitude of tube effects. We filled tubes with only 1 mL of control material so as to produce high concentrations of additive and thus maximize any possible effects of the additives on the assays investigated in this study.

Our results emphasize the benefit of strict adherence to one of the basic tenets of QC, namely, that control specimens should be treated identically to specimens from patients. This strategy has revealed the interference attributable to the additives in the variant lot of SST tubes and, had it been in place, would have alerted the laboratory to the interference. We recommend that laboratories consider including this strategy in their QC plan. Periodic or routine processing of controls in blood collection tubes should provide a timely warning of possible interferentes by additives in blood collection tubes.

References

(1.) Bowen RAR RAR Retinoic Acid Receptor
RAR Resource Adapter Archive (J2EE)
RAR Royal Australian Regiment
RAR Risk Assessment Report
RAR Roshal Archive (WinRAR compressed file format; file extension) 
, Chan Y, Cohen cohen
 or kohen

(Hebrew: “priest”) Jewish priest descended from Zadok (a descendant of Aaron), priest at the First Temple of Jerusalem. The biblical priesthood was hereditary and male.
 J, Rehak NN, Hortin GL, Csako G, et al. Effect of blood collection tubes on total triiodothyronine and other laboratory assays. Clin Chem 2005;51:424-33.

(2.) Holmes DT, Levin A, Forer B, Rosenberg F. Preanalytical influences on DPC IMMULITE 2000 Intact PTH PTH
abbr.
parathyroid hormone


Parathyroid hormone (PTH)
A chemical substance produced by the parathyroid glands. This hormone is a major element in regulating calcium in the body.
 assays of plasma and serum from dialysis patients. Clin Chem 2005;51:915-7.

(3.) Parham S. Test tube interference shows "chink in armor". CAP Today 2005;19:1.

(4.) Becton-Dickinson[TM]. BD assay global interference communication.

Technical bulletin VS7313. http://www.bd.tom/vacutainer/techbulletins/ (September 3, 2004).

(5.) Bowen RAR, Chan Y, Ruddel ME, Hortin GL, Csako G, Demosky SJ Jr, et al. Immunoassay interference by a commonly used blood collection tube additive, the organosilicone surfactant Silwet L-720. Clin Chem 2005;51:1874-82.

(6.) Kricka U, Park JY. Additive-aggravated assays: an authoritative answer [Editorial]. Clin Chem 2005;51:1767.

Larry J. Kricka *

Jason Y. Park

Marilyn B. Senior

Rodelia Fontanilla

Department of Pathology and Laboratory Medicine Hospital of the University of Pennsylvania (body, education) University of Pennsylvania - The home of ENIAC and Machiavelli.

http://upenn.edu/.

Address: Philadelphia, PA, USA.
 Philadelphia, PA

* Address correspondence to this author at: Hospital of the University of Pennsylvania, Department of Pathology and Laboratory Medicine, 3400 Spruce St., Philadelphia, PA 19104-4283. Fax 215-662-7529; e-mail kricka@mail.med.upenn.edu.

DOI (Digital Object Identifier) A method of applying a persistent name to documents, publications and other resources on the Internet rather than using a URL, which can change over time. : 10.1373/clinchem.2005.060392
Table 1. Results for controls analyzed after exposure to the variant
lot of SST tubes. (a)

                   Control          Result        QC interval
                   (b)

Total [T.sub.4],   A         31.9   35.1   31.9   11.8-27.8
[micro]g/L
                   B         61.9   68.4   74.2     31-47
                   D         215    220    199     128-180
Total [T.sub.3],   B         0.97   1.00   1.15   0.63-1.03
[micro]g/L
                   C         2.70   2.49   2.23   1.80-2.44
                   D         4.22   4.57   4.26   3.36-4.64
Cortisol,          B         31.1   34.1   30.3   19.4-31.8
[micro]g/L
                   C         241    209    235     136-304
                   D         375    412    371     241-401
FSH, IU/L          A         2.20   2.30   2.33    2.3-3.1
                   C         11.3   10.8   11.4   11.9-15.1
                   D         65.1   66.0   67.3   64.9-80.9

(a) Results at or outside the established QC range are shown in
italics.

(b) Controls: Bio-Rad Lyphochek Immunoassay Plus Control lot 40160.
A, Bio-Rad control 1 reconstituted with double the amount of deionized
water; B, Bio-Rad control 1; C, Bio-Rad control 2; D, Bio-Rad
control 3.
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Title Annotation:Letters
Author:Kricka, Larry J.; Park, Jason Y.; Senior, Marilyn B.; Fontanilla, Rodelia
Publication:Clinical Chemistry
Article Type:Letter to the editor
Date:Dec 1, 2005
Words:1309
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