Predation potential of the invasive green crab (Carcinus maenas) and other common predators on commercial bivalve species found on Prince Edward Island.ABSTRACT The prey and size-class preferences of four marine epibenthic predators was examined in a laboratory study by determining the mortality rates of four commercial bivalve bivalve, aquatic mollusk of the class Pelecypoda ("hatchet-foot") or Bivalvia, with a laterally compressed body and a shell consisting of two valves, or movable pieces, hinged by an elastic ligament. species in 4-d trials where predators were present or absent. Bivalve species used were quahogs (Mercenaria mercenaria), eastern oysters The eastern oyster, Crassostrea virginica, also known as the American oyster, Atlantic oyster, or the Virginia oyster, is a species of oyster that is native to the eastern seaboard of North America. (Crassostrea virginica), blue mussels The blue mussel, here specifically Mytilus edulis, is a medium-sized edible bivalve mollusc. It is commonly harvested for food throughout the world, from both wild and farmed sources. (Mytilus edulis) and soft-shell clams (Mya arenaria). Prey size-classes investigated were 0-15, 15-25 and 25-40 mm. A first set of experiments compared the mortality rates of prey in the presence of the invasive green crab (Zool.) an edible, shore crab (Carcinus menas) of Europe and America; - in New England locally named joe-rocker.
See also: Green (Carcinus maenas Carcinus maenas is a common littoral crab, and an important invasive species. It is listed among the 100 "world's worst invasive alien species" .
C. maenas is known by different names around the world. ) and the native rock crab (Cancer irroratus). The second set of experiments compared the predation predation
Form of food getting in which one animal, the predator, eats an animal of another species, the prey, immediately after killing it or, in some cases, while it is still alive. Most predators are generalists; they eat a variety of prey species. behavior of the green crab, the common starfish The common starfish (Asterias rubens) is the most common and familiar starfish in the north-east Atlantic. It has five arms and usually grows to between 10-30cm across, although larger specimens (up to 52cm across) are known. (Asterias vulgaris) and the moon snail (Euspira heros). Single- and multiple-choice experiments were carried out in relation to the prey species being challenged by the predator. Results from the first set of experiments showed that green crabs preyed on all prey species from all size-classes in single and multiple-choice trials. Mussels and clams were the preferred prey species. Rock crabs preyed on mussels in single- and multiple-choice trials. Rock crabs preyed heavily on soft-shell clams in single-choice trials, whereas no clams were eaten in multiple-choice trials. Rock crabs did not prey on quahogs in single- and multiple-choice trials. In both trials, small individuals were preyed on more often by both crab species. The second set of experiments confirmed results observed for the green crab in the first set of experiments. The common starfish was a very active predator as well. Blue mussels were the preferred prey species of the starfish. Moon snails displayed a much lower predation activity than the other predators. The multiple-choice experiment showed different results in that predation rates were lower than in cases where predators were facing one prey species at a time. Overall, our results showed that the two crab species and the common starfish displayed a generalist gen·er·al·ist
A physician whose practice is not oriented in a specific medical specialty but instead covers a variety of medical problems.
generalist feeding behavior. Results also showed that the invasive green crab might represent a new predation threat to commercial bivalves as well as a competing threat to the native rock crab.
KEY WORDS: predation, crab, Carcinus maenas, Cancer irroratus, starfish, Asterias vulgaris, snail, Euspira heros, aquaculture aquaculture, the raising and harvesting of fresh- and saltwater plants and animals. The most economically important form of aquaculture is fish farming, an industry that accounts for an ever increasing share of world fisheries production. , bivalve
The culture of bivalve species represents one of the most important aquaculture industries in Atlantic Canada. In the year 2003, about 80% of the total bivalve aquaculture production originated from Prince Edward Island Prince Edward Island, province (2001 pop. 135,294), 2,184 sq mi (5,657 sq km), E Canada, off N.B. and N.S. Geography
One of the Maritime Provinces, Prince Edward Island lies in the Gulf of St. (PEI) (Department of Fisheries fisheries. From earliest times and in practically all countries, fisheries have been of industrial and commercial importance. In the large N Atlantic fishing grounds off Newfoundland and Labrador, for example, European and North American fishing fleets have long and Oceans 2004). The most important species cultivated in PEI are the blue mussel (Mytilus eclulis) and the eastern oyster (Crassostrea virginica) (Department of Fisheries and Oceans 2004). The PEI shellfish shellfish, popular name for certain edible mollusks (see Mollusca), e.g., oysters, clams, and scallops, and for certain edible crustaceans, e.g., crabs, lobsters, and shrimps. All are aquatic invertebrates with shells; they are not fish. industry has also shown, over the last decade, an increasing interest in diversifying cultured bivalve species (Brown et al. 1995). Two new native species, the quahog quahog: see clam.
Thick-shelled edible clam of the U.S. The northern quahog (Mercenaria mercenaria), also known as the cherrystone, littleneck, or hard-shell clam, is 3–5 in. (8–13 cm) long. (Mercenaria mercenaria) and the soft-shell clam (Mya arenaria), are presently undergoing grow-out trials by the aquaculture industry.
Predators play an important role in structuring marine benthic ben·thos
1. The collection of organisms living on or in sea or lake bottoms.
2. The bottom of a sea or lake.
[Greek. communities (see reviews by Connell 1983, Commito & Ambrose 1985, Menge & Sutherland 1987, Ambrose 1991, Ebenhoh et al. 1995). Studies have identified a large spectre of endo- and epibenthic predators in soft- and hard-bottom communities including invertebrates (e.g., polychaetes, nemerteans, crabs, gastropods, echinoderms) and vertebrates (e.g., fish, birds, mammals). Because most bivalve aquaculture operations occur directly in the marine environment, cultured animals are vulnerable to predators. Predation at culture sites is a major concern of bivalve farmers (e.g., Lindsay & Savage 1978, Flimlin 1993, Rosenthal et al. 1995, Beal & Krauss 2002, Walton et al. 2002). For instance, commercial bivalve aquaculture ventures in the United States United States, officially United States of America, republic (2005 est. pop. 295,734,000), 3,539,227 sq mi (9,166,598 sq km), North America. The United States is the world's third largest country in population and the fourth largest country in area. spend a fair proportion of their budget every year in antipredation material and techniques to control quahog mortality caused by predators (Menzel 1989).
Many potential predators were identified by PEI shellfish farmers and various stakeholders Stakeholders
All parties that have an interest, financial or otherwise, in a firm-stockholders, creditors, bondholders, employees, customers, management, the community, and the government. to be threats to their operations (Prince Edward Island Aquaculture Alliance 2000). These predators include the common starfish (Asterias vulgaris), the moon snail (Euspira heros) and various crab species including the green crab (Carcinus maenas), which was accidentally introduced to North America North America, third largest continent (1990 est. pop. 365,000,000), c.9,400,000 sq mi (24,346,000 sq km), the northern of the two continents of the Western Hemisphere. in the 19th century (see review from Audet et al. 2003) and the rock crab (Cancer irroratus). The green crab was noted for the first time off southeastern shores of PEI in 1997 (Gillis et al. 2000, Audet et al. 2003). It has since progressed toward the northwest of the island on both shores (Audet et al. 2003). Wherever they are found, green crabs have been shown to affect their new environment at various ecologic levels (e.g., Glude 1955, Ropes 1968, Hughes & Elner 1979, Elner 1981, Grosholz et al. 2000, Trussel & Smith 2000, McDonald et al. 2001, Walton et al. 2002, Floyd & Williams 2004).
Without adequate knowledge of the biology and the impact of these predators on cultured bivalve populations, little can be done to suggest antipredation techniques. The objective of this study is to evaluate and document the predation behavior of various species to understand and establish the priority of implementing predator control measures and further research and development projects. A first experiment was carried out by determining mortality rates of four PEI commercial bivalve species in the presence and absence of the invasive green crab and the native rock crab. A complementary experiment was also undertaken to compare the predation potential of the green crab to other predators commonly found off the coast of PEI (common starfish and moon snail). It was expected that mortality rates of the prey species would increase in the presence of predators, would vary according to according to
1. As stated or indicated by; on the authority of: according to historians.
2. In keeping with: according to instructions.
3. the predator species and mortality rates per predator species would decrease with increasing prey size.
MATERIALS AND METHOD
Experimental Enclosures and Prey Species
All experiments were carried out at the Ellerslie Shellfish Hatchery hatchery
a commercial establishment dedicated to the hatching of bird eggs to provide day old chicks and poults to the poultry industry.
the contents of unfertilized eggs. Used in petfood manufacture. , PEI (Canada), in the fall of 2000. Trials were held in thirty 38-cm wide x 54-cm long x 16-cm high plastic crates. These crates were divided in two with a PVC PVC: see polyvinyl chloride.
in full polyvinyl chloride
Synthetic resin, an organic polymer made by treating vinyl chloride monomers with a peroxide. separator tightly fixed with silicon. This gave us a total of 60 water-tight experimental enclosures (38-cm wide x 27-cm long). Each side opposing the PVC separator had a small hole for water outflow (1 cm from the crate rim). Enclosures were filled with a 10-cm-deep layer of homogeneous sandy sediments. Sediments were sieved through a l-mm mesh prior to use (removal of macro-invertebrates) but were not sterilized ster·il·ize
tr.v. ster·il·ized, ster·il·iz·ing, ster·il·iz·es
1. To make free from live bacteria or other microorganisms.
2. . Crates were randomly placed in 2 large tanks (15 per tank). These tanks allowed us to collect the water outflow from all crates and insured that the invasive green crabs could not escape inside the hatchery. Experimental enclosures were individually supplied with seawater seawater
Water that makes up the oceans and seas. Seawater is a complex mixture of 96.5% water, 2.5% salts, and small amounts of other substances. Much of the world's magnesium is recovered from seawater, as are large quantities of bromine. . The water was pumped from the Bideford Estuary estuary (ĕs`chĕr'ē), partially enclosed coastal body of water, having an open connection with the ocean, where freshwater from inland is mixed with saltwater from the sea. (20-23 ppt ppt
1. parts per thousand
2. parts per trillion ) through a sand filter. The water was then heated to 15[degrees]C in holding bins and then supplied to the experimental enclosures by gravity. Experimental enclosures were left for 1 wk to allow the sediments to stabilize.
Animals were obtained from various PEI locations: eastern oysters from Malpeque Bay Malpeque Bay is a 244 km² shallow coastal lagoon on the northern shores of Prince Edward Island in Canada, about 10 km north of the town of Summerside. It was classified as a wetland of international importance via the Ramsar Convention on April 28, 1988. , soft-shell clams from North River and quahogs from the Ellerslie hatchery. Quahogs were of the notata variety. Three size-classes were used to determine the preferred size-classes of predators: 0-15 mm, 15-25 mm and 25-40 mm. Green crabs and rock crabs for experiment 1 were collected from Pinette River and Basin Head, respectively. Green crabs and moon snails were collected from St. Mary's Bay, whereas common starfish were from Tracadie Bay in experiment 2. In both experiments, only male crabs were used. Green crabs in prolonged intermolt phase (orange or red coloration col·or·a·tion
1. Arrangement of colors.
2. The sum of the beliefs or principles of a person, group, or institution. ) were used to eliminate potential carapace carapace (kâr`əpās), shield, or shell covering, found over all or part of the anterior dorsal portion of an animal. In lobsters, shrimps, crayfish, and crabs, the carapace is the part of the exoskeleton that covers the head and thorax weakness in recently molted crabs (green coloration). Bivalves were deposed on the sediments in each enclosure 24 h before predators were introduced (1 predator per enclosures except in control treatments). This allowed the infaunal species an opportunity to burrow into the sediments. The sediment layer was thick enough to allow quahogs and soft-shell clams to cover themselves completely and extend their siphon siphon (sī`fən, –fŏn), tube through which a liquid is lifted over an elevation by the pressure of the atmosphere and is then emptied at a lower level. without being exposed. Small-size mesh nets (1 cm aperture) were affixed af·fix
tr.v. af·fixed, af·fix·ing, af·fix·es
1. To secure to something; attach: affix a label to a package.
2. using clips on enclosures to prevent predators from escaping. Photoperiod photoperiod /pho·to·pe·ri·od/ (fo´to-per?e-od) the period of time per day that an organism is exposed to daylight (or to artificial light).photoperiod´ic
n. was maintained at 8 h of light and 16 h of darkness in all experiments.
Predation Trials Between Green Crabs and Rock Crabs
Trials were designed to have one of the crab species combined with one of the four prey species at a time (single-choice experiment). Ten individuals per size-class were used for a total of 30 prey per single experimental enclosure. Another set of trials was designed to combine one of the crab species (1 individual) with all the prey species within the same enclosure (multiple-choice experiment). Crabs were not fed for a 24-h period before their introduction into the enclosures. A total of five different prey treatments were used. Three different predator treatments, including controls (no predator), were also used. All trials were replicated four times for a total of 60 experimental enclosures (3 predator treatments x 5 prey treatments x 4 replicates). Three individuals per size-class were used in enclosures bearing all the prey species for a total of 36 individuals. Predator-prey combinations were randomly assigned to the experimental enclosures. Green and rock crabs weighted 61.43 [+ or -] 10.51 g and 103.89 [+ or -] 29.27 g (mean [+ or -] SD, n = 20), respectively. All enclosures were sieved 4 d after the introduction of crabs and the mortality rate of bivalves was determined.
Predation Trials Between Green Crabs, Common Starfish and Moon Snails
The experimental design in experiment 2 was similar to the one described in experiment 1. Trials were designed to have single- and multiple -choice experiments. Prey densities were the same as described in experiment 1. Five different prey treatments were used. Four different predator treatments, including controls (no predator), were also used. All trials were replicated three times for a total of 60 experimental enclosures (4 predator treatments x 5 prey treatments x 3 replicates). Individuals, within each predator group, had a similar weight (mean [+ or -] SD, n = 15): green crab: 59.47 [+ or -] 9.14 g; common starfish: 8.93 [+ or -] 3.69 g; moon snail: 15.13 [+ or -] 2.26 g. Again, all enclosures were sieved 4 d after the introduction of predators and the mortality rate of bivalves was determined.
The data were analyzed with SPSS A statistical package from SPSS, Inc., Chicago (www.spss.com) that runs on PCs, most mainframes and minis and is used extensively in marketing research. It provides over 50 statistical processes, including regression analysis, correlation and analysis of variance. 11.5 for Windows. All analyses were calculated at a confidence level of P < 0.05. Mortality rates for each prey species were arcsin transformed to meet normality normality, in chemistry: see concentration. and homoscedasticity requirements and analyzed with a 3-way ANOVA anova
see analysis of variance.
ANOVA Analysis of variance, see there in each experiment. The factors were predator species, prey species and size-class. Control data were not included in the ANOVA models because of the numerous zero values (high survival rates of prey in the absence of predators). Tukey multiple comparisons were applied when significant differences were found on factors.
Predation Trials Between Green Crabs and Rock Crabs
Single-choice Experiment The green crab preyed on all species and all size-classes except for large eastern oysters (25-40 mm size-class) (Fig. 1A). Mean mortality rates for each species were, however, all under the 50% level. Blue mussels and soft-shell clams were the preferred prey species. For all prey species, small individuals (0-15 mm) were preyed on more heavily. Overall, results showed that about 21% of all prey available was eaten by the green crabs (all trials, all species and all sizes confounded), from which 35% and 27% of mussels and clams, respectively, were eaten (all trials and all sizes confounded). The overall numbers also showed (all trials and all species confounded) that 35% of the 0-15 mm individuals were eaten compared with 20% for the 15-25 mm individuals and 8% for the 25-40 mm individuals. Mortality patterns were similar for rock crabs in the single-choice experiment (Fig. 1A). Again, results showed that rock crabs preferred clams and mussels and that small size individuals were preyed more heavily. For instance, almost 80% of the small clams (0-15 mm) were eaten. Except for this particular case, mean mortality rates were, however, all under the 40% level. Rock crabs did not prey on quahogs. Overall, results showed that 22% of all prey was eaten by the rock crabs (all trials, all species and all sizes confounded), from which 47% and 28% of clams and mussels, respectively, were eaten (all trials and all sizes confounded). The overall numbers also showed (all trials and all species confounded) that 38% of the 0-15 mm individuals were eaten compared with 14% for the 15-25 mm individuals and 13% for the 25-40 mm individuals. A low number of individuals died in the control treatments during the single-choice experiment. All clams survived. The ANOVA analysis (Table 1) confirmed that both crab species preferred small individuals of soft-shell clams and blue mussels. No interaction effect was observed.
[FIGURE 1 OMITTED]
Multiple-choice Experiment As observed in the single-choice experiment, green crabs preyed on all species and all size-classes (Fig, 1B). Mean mortality rates were over 50% in various cases: mussels and oysters <25 mm and small (0-15 mm) clams. Mussels and oysters were the preferred prey species. Overall, results showed that 38% of all prey available was eaten by the green crabs (all trials, all species and all sizes confounded) compare with 21% in the single-choice experiment. About 64% and 50% of mussels and oysters, respectively, were eaten (all trials and all sizes confounded). The overall numbers also showed (all trials and all species confounded) that 50% of the 0-15 mm individuals were eaten compared with 52% for the 15-25 mm individuals and 13% for the 25-40 mm individuals. In other words Adv. 1. in other words - otherwise stated; "in other words, we are broke"
put differently , about 83% of mussels and 75% of oysters <25 mm were eaten by the green crabs compared with 58% of the small clams (0-15 mm) in the multiple-choice experiment. Rock crabs preyed more on mussels and oysters as well, except for large oysters (Fig. 1B). No quahogs were preyed on. In contrast to results observed in the single-choice experiment, clams were not preyed on by rock crabs. Overall, results showed that only 11% of all prey available was eaten by rock crabs (all trials, all species and all sizes confounded), from which 33% and 8% of mussels and oysters, respectively, were eaten (all trials and all sizes confounded). Only 1 soft-shell clam of the 27 specimens exposed was eaten. This is in contrast to results observed in the single-choice experiment where about 47% of clams were eaten by rock crabs. All individuals survived in the control treatments. The ANOVA analysis (Table 2) confirmed two interaction effects. Overall, the low mortality rates observed for the clams in rock crab treatments probably explained a large proportion of the variability related to prey species x size-class and predator species x size-class interactions as well as the significant effect from the predation species.
Predation Trials Between Green Crabs, Common Starfish and Moon Snails
Single-choice Experiment Results from this experiment confirmed that green crabs preyed on all the studied prey species (Fig. 2A). Blue mussels and soft-shell clams were again the first and second preferred second preferred
A class of preferred stock that has a subordinate claim to dividends and assets relative to another class of preferred stock of the same issuer. Compare prior preferred. prey species, respectively. The relation with the size of prey was however not as clear as in Experiment 1. This was particularly true with mussels and clams. The common starfish was also very active during the trials (Fig. 2A). Mussels, as observed in green crab treatments, were the preferred prey species. The eastern oyster, however, represented the second preferred prey species. The starfish did not prey on other prey species. Again, small individuals were preyed on more heavily. The moon snail was the least active predator (Fig. 2A). No predation was observed on quahogs and oysters. Mean mortality rates for mussels and clams were below 10%. Mortality rates in control treatments were 0% regardless of the size-class for all prey species, except for clams (Fig. 2A). Clams suffered low mortality in all size-classes (ca 5%). This means there could be a slight overestimation o·ver·es·ti·mate
tr.v. o·ver·es·ti·mat·ed, o·ver·es·ti·mat·ing, o·ver·es·ti·mates
1. To estimate too highly.
2. To esteem too greatly. of mortality rates for clams. The ANOVA analysis (Table 3) underlined a strong predator species x prey species x size-class interaction.
[FIGURE 2 OMITTED]
Multiple-choice Experiment Trials where all prey species were challenged with a single predator showed different results from what was observed in the previous single-choice experiment. The green crab preyed on all prey species except for quahogs, whereas the common starfish preyed on all prey species including small-size quahogs (Fig. 2B). The moon snail was again the least active predator. Large-size quahogs were, however, preyed on in the moon snail trials. Low mortality rates were observed in small-size oysters and mussels and midsize clams (<5%) in the control treatments. The overall effect of size-class was not as clear as observed in the other experiments. The ANOVA analysis (Table 4) failed to show any significant effect from predator species, prey species and size-class. No interaction was observed.
The present laboratory experiments suggest that the green crab, rock crab and common starfish may be important predators in bivalve aquaculture sites off PEI shores. Single-choice experiments showed that blue mussels were selected more often by these predators compared with moon snails. This was particularly true for small-size individuals (<25 mm). O'Neill et al. (1983) and ap Rheinalt (1986) documented similar results with seastars and crabs (including green crabs), respectively, versus cultivated blue mussels. This suggests that large mussels probably attained a partial prey refuge in size (at ~25 mm) from predators. Several authors came to the same conclusions while studying the relationship between various species of crabs and bivalve species, including the ones between the green crab and the blue mussel (e.g., Elner 1980, Townsend & Hughes 1981, Navarrete & Castilla 1988, Eggleston 1990, Juanes 1992, MacNair, personal observation) and soft-shell clam (e.g., Welch 1968, Cohen cohen
(Hebrew: “priest”) Jewish priest descended from Zadok (a descendant of Aaron), priest at the First Temple of Jerusalem. The biblical priesthood was hereditary and male. et al. 1995, Beal et al. 2001, Floyd & Williams 2004). Large-size prey may be more difficult to handle by crabs (manipulation with the chela che·la
n. pl. che·lae
A pincerlike claw of a crustacean or arachnid, such as a lobster, crab, or scorpion.
[New Latin ch ) as well as being more difficult to open by seastars (large prey have stronger adductor muscles Noun 1. adductor muscle - a muscle that draws a body part toward the median line
skeletal muscle, striated muscle - a muscle that is connected at either or both ends to a bone and so move parts of the skeleton; a muscle that is characterized by ). This response may, however, vary in relation to the size of the predator. Eggleston (1990), for instance, observed that large-size crabs feed on large eastern oysters (>45 mm) because larger individuals display an increased crushing strength. Furthermore, male crabs exhibit a larger crusher chela height, and in turn, more strength, than a female of the same year class (Hines et al. 1990).
Soft-shell clams and quahogs are both infaunal species. The fact that both species bury themselves in the sediments may provide them a spatial refuge from some predators. However, our results suggest that crabs were able to detect and handle soft-shell clams without any difficulty. The shape and hardness of quahogs may offer some protection from predators compared with softshell clams that have a weaker shell (Blundon & Kennedy 1982a, Boulding 1984) as well as exposed siphon and pedal gapes (Dare & Edwards 1981, Boulding 1984). Burial depth, for soft-shell clams, is apparently the only refuge from epibenthic predators (Blundon & Kennedy 1982b, Whitlow whitlow /whit·low/ (hwit´lo) felon.
herpetic whitlow primary herpes simplex infection of the terminal segment of a finger, with extensive tissue destruction, sometimes accompanied by systemic et al. 2003). Because the green crab may dig pits up to 15 cm deep (Ropes 1968, Lindsay & Savage 1978), the predation level observed during this study may be higher than in nature where burial depth is not limited. This may be particularly true for the large individuals. Large soft-shell clams have the tendency to be found deeper (ca 15 cm) in the sediment compare with small individuals (Zaklan & Ydenburg 1997).
The multiple-choice experiment suggests that the predation behavior of green crab and common starfish may be more general when several prey species are challenged at the same time. Results showed that prey species were more homogeneously selected compared with single-choice trials. This was particularly true for starfish that also preyed on small-size quahogs. Various feeding studies showed that green crabs might feed on various taxonomic groups Noun 1. taxonomic group - animal or plant group having natural relations
taxon, taxonomic category
Adapid, Adapid group - extinct small mostly diurnal lower primates that fed on leaves and fruit; abundant in North America and Europe 30 to 50 million years including polychaetes, gastropods and barnacles (e.g., Le Calvez 1984, Williams 1984). Field trials conducted in 2000 by the Department of Fisheries, Aquaculture and Environment of PEI (DFAE DFAE Département Fédéral des Affaires Étrangères (French: Federal Department of Foreign Affairs; Switzerland)
DFAE Director of Facilities Engineering ) showed, however, that in multiple-choice experiments with green crabs, mussels were the preferred food over soft-shell clams and oysters (MacNair, pers. comm.). Though they were able to find and eat buried soft-shell clams in the single-choice experiment, rock crabs preferred mussels and oysters in the multiple-choice experiment. Our results also showed that rock crabs might not be as efficient when facing multiple prey species at the same time. This suggests that the rock crab might spend more time searching for certain prey species compared with the green crab, which seems to exhibit opportunistic behavior.
Single-choice and multiple-choice experiments suggest that moon snails were the least active predator. Observed predation rates were generally low for all prey species. This was a surprising result because soft-shell clams and blue mussels are reported to be heavily preyed upon by this gastropod gastropod, member of the class Gastropoda, the largest and most successful class of mollusks (phylum Mollusca), containing over 35,000 living species and 15,000 fossil forms. (Vencile 1997). The percentage of live clams demonstrating moon snail drill holes, however, was high in this study. This was also the case in the DFAE field study carried in 2000 (MacNair, pers. comm.). A longer experimental period probably would give a higher mortality rate than that observed in the laboratory.
Conclusion and Recommendations
Our results confirmed that the native rock crab and common starfish, as well as the invasive green crab, are potential threat for various PEI commercial bivalves, particularly for small-size individuals (<25 mm). The moon snail did not appear to be an important predator except for large-size quahogs (25-40 ram) and midsize clams (15-25 mm). Our results suggest that the status of the green crab around the coasts of PEI should continue to be monitored yearly and that PEI shellfishery shell·fish·er·y
n. pl. shell·fish·er·ies
1. The industry or occupation of catching, processing, or selling shellfish.
2. A fishing ground for shellfish. operations should direct their effort in protecting small-size individuals from predators until they reach a size refuge. This is particularly true for ventures that harvest commercial species in a natural setting where predators have a direct access to their prey. Laboratory and field experiments should be designed to test antipredation techniques adapted to specific aquaculture operations. Predator exclusion by fencing is a possibility in the intertidal in·ter·tid·al
Of or being the region between the high tide mark and the low tide mark.
in habitat, The use of certain prey species to redirect predators away from a given species may also bear interesting results for infaunal species harvested in tidal flats tidal flat
Level muddy surface bordering an estuary, alternately submerged and exposed to the air by changing tidal levels. In addition to the alternating submergence and exposure, the varying influences of fresh river water and salty marine waters cause physical conditions as well as for species suspended above the sea floor. For instance, our results tend to show that mussels could be used by farmers to attract predators and reduce oysters or soft-shell clam mortalities. The development of a fishery for green crabs could also help to diminish economic losses as well as to generate direct revenues. Small newly-molted green crabs, for instance, are in high demand in many Mediterranean countries (Peter J. W. Olive, pers. comm.).
The similar feeding diet between green crabs and rock crabs as underlined in the single choice-experiment show that green crabs may represent a competing threat to rock crabs. Though green crabs are usually found in more brackish brack·ish
1. Having a somewhat salty taste, especially from containing a mixture of seawater and fresh water: "You could cut the brackish winds with a knife/Here in Nantucket" tidal environments, green crabs may be also found in other types of environment including the ones of the rock crabs (Neal MacNair, pers. comm., Dominique Audet, personal observation). Their ubiquitous distribution, diversified feeding diet, tolerance to extreme field conditions and their more aggressive behavior may give them an advantage over the rock crabs. Studies should now look more precisely at the relationship between these two species in the context of a boreal bo·re·al
1. Of or relating to the north; northern.
2. Of or concerning the north wind.
3. Boreal environment (e.g., feeding and agonistic agonistic /ag·o·nis·tic/ (ag?o-nis´tik) pertaining to a struggle or competition; as an agonistic muscle, counteracted by an antagonistic muscle. behavior in relation to water temperature). The development of a green crab fishery could also help the native rock crab.
The authors thank Daniel Bourque, Angeline LeBlanc and Bruno Frenette for their help in the field and in the laboratory; also Roy Winn and particularly Paul Burleigh who greatly facilitated their lives at the hatchery. Comments provided by two anonymous referees helped improve the clarity of the text. Funding for this research was provided by a research contract from the PEI Aquaculture & Fisheries Research Initiative (AFRI AFRI Arid Forest Research Institute (India)
AFRI Approved Force Retention Increment
AFRI American Fund Raising Institute, Inc. ) to G. M. and T. L. and a Universite de Moncton (Faculte des etudes superieures et de la recherche La Recherche is a monthly French language popular science magazine covering recent scientific news. It is published by the Société d'éditions scientifiques (the Scientific Publishing Group), a subsidiary of Financière Tallandier. ) grant to G. M. The Department of Fisheries and Oceans and Universite de Moncton provided internships to D. A.
Ambrose, W. G., Jr. 1991. Are infaunal predators important in structuring marine soft-bottom communities? Am. Zool. 31:849-860.
ap Rheinalt, T. 1986. Size-selection by the crab Liocarcinus puber feeding on mussels Mytilus and on shore crabs shore crab
Any of numerous crabs, such as the spider crab of the United States or the common edible crab Carcinus maenas, usually found along seashores. , Carcinus maenas: the importance of mechanical factors. Mar. Ecol. Prog. Ser. 29:45-53.
Audet, D., D. Davis, G. Miron, M. Moriyasu, K Benhalima & R. Campbell. 2003. Geographical expansion of a nonindigenous crab, Carcinus maenas (L.), along the Nova Scotian No·va Sco·tia Abbr. NS or N.S.
A province of eastern Canada comprising a mainland peninsula and the adjacent Cape Breton Island. It joined the confederation in 1867. shore into the southeastern Gulf of St. Lawrence Noun 1. Gulf of St. Lawrence - an arm of the northwest Atlantic Ocean off the southeastern coast of Canada
Gulf of Saint Lawrence
Atlantic, Atlantic Ocean - the 2nd largest ocean; separates North and South America on the west from Europe and Africa on the east , Canada. J. Shellfish Res. 22:255-262.
Beal, B.F., M.R. Parker & K.W. Vencile. 2001. Seasonal effects of intraspecific in·tra·spe·cif·ic also in·tra·spe·cies
Arising or occurring within a species: intraspecific competition. density and predator exclusion along a shore-level gradient on survival and growth of smalls of the soft-shell clam, Mya arenaria L., in Maine. USA. J. Exp. Mar. Biol. Ecol. 264:133-169.
Beal, B. F. & M. G. Krauss. 2002. Interactive effects of initial size, stocking density, and type of predator deterrent netting on survival and growth of cultured juveniles of the soft-shell clam, Mya arenaria L., in eastern Maine. Aquaculture 208:81-111.
Blundon, J.A. & V. S. Kennedy. 1982a. Mechanical and behavioral aspects of the blue crab blue crab, common name for a crustacean, Callinectes sapidus, found on the S Atlantic and Gulf coasts of North America. The blue crab is a member of the family of swimming crabs known as the Portunidae and is characterized by a broad, semitriangular carapace , Callinectes sapidus (Rathbun), predation on Chesapeake Bay Chesapeake Bay, inlet of the Atlantic Ocean, c.200 mi (320 km) long, from 3 to 30 mi (4.8–48 km) wide, and 3,237 sq mi (8,384 sq km), separating the Delmarva Peninsula from mainland Maryland. and Virginia. bivalves. J. Exp. Mar. Biol. Ecol. 65:47-65.
Blundon, J.A. & V. S. Kennedy. 1982b. Refuges for infaunal bivalves from the blue crab, Callinectes sapidus (Rathbun), predation in Chesapeake Bay. J. Exp. Mar. Biol. Ecol. 65:67-81.
Boulding, E. G. 1984. Crab-resistant features of shells of burrowing bivalves: decreasing vulnerability by increasing handling time. J. Exp. Mar. Biol. Ecol. 76:201-223.
Brown, J., M. Helm & J. Moir. 1995. New-candidate species for aquaculture. In: A. D. Boghen, editor. Cold-water aquaculture in Atlantic Canada. The Canadian Institute for Research on Regional Development. pp. 341-362.
Cohen, A. N., J. T. Carlton & M. C. Fountain. 1995. Introduction, dispersal and potential impacts of the green crab Carcinus maenas in San Francisco Bay San Francisco Bay, 50 mi (80 km) long and from 3 to 13 mi (4.8–21 km) wide, W Calif.; entered through the Golden Gate, a strait between two peninsulas. , California. Mar. Biol. 122:225-237.
Connell, J. 1983. On the prevalence and relative importance of interspecific competition Interspecific competition, in ecology, is a form of competition in which individuals of different species vie for the same resource in an ecosystem (e.g. food or living space). : evidence from field experiments. Am. Nat. 122:661-696.
Commito, J. A. & W. G. Ambrose, Jr. 1985. Predatory infauna in·fau·na
Aquatic animals that live in the substrate of a body of water, especially in a soft sea bottom.
[in-2 + fauna. and trophic trophic /tro·phic/ (tro´fik) (trof´ik) pertaining to nutrition.
Of, relating to, or characterized by nutrition. complexity in soft-bottom communities. In: P. E. Gibbs, editor. Proceedings of the nineteenth European Marine Biology marine biology, study of ocean plants and animals and their ecological relationships. Marine organisms may be classified (according to their mode of life) as nektonic, planktonic, or benthic. Nektonic animals are those that swim and migrate freely, e.g. Symposium. Cambridge, England: Cambridge University Press Cambridge University Press (known colloquially as CUP) is a publisher given a Royal Charter by Henry VIII in 1534, and one of the two privileged presses (the other being Oxford University Press). . pp. 323-333.
Dare, P. J. & D. B. Edwards. 1981. Underwater television observations on the intertidal movements of shore crabs, Carcinus maenas (L.), across a mudflat Mudflats are coastal wetlands that form when mud is deposited by the tides or rivers, sea and oceans. They are found in sheltered areas such as bays, bayous, lagoons, and estuaries. . J. Mar Biol. Ass. U.K 61:107-116.
Department of Fisheries and Oceans. 2004. 2003 Canadian aquaculture production statistics. Available at: http://www.dfompo.gc.ca/ communic/statistics/aqua/aqua03.htm
Ebenhoh, W., C. Kohlmeier & P. J. Radford. 1995. The benthic ecological submodel in the European regional seas ecosystem model Ecosystem models, or ecological models, are mathematical representations of ecosystems. Typically they simplify complex foodwebs down to their major components or trophic levels, and quantify these as either numbers of organisms, biomass or the inventory/concentration of . Neth. J. Sea Res. 33:423-452.
Eggleston, D. B. 1990. Functional responses of blue crab Callinectes sapidus Rathbun feeding on juvenile oysters Crassostrea virginica (Gmelin): effects of predator sex and size, and prey size. J. Exp. Mar. Biol. Ecol. 143:73-90.
Elner, R.W. 1980. The influence of temperature, sex and chela size in foraging strategy of the shore crab, Carcinus maenas (L.). Mar. Behav. Physiol. 7:15-24.
Elner, R.W. 1981. Diet of green crab Carcinus maenas (L.) from Port Hebert, southwestern Nova Scotia Nova Scotia (nō`və skō`shə) [Lat.,=new Scotland], province (2001 pop. 908,007), 21,425 sq mi (55,491 sq km), E Canada. Geography
. J. Shellfish Res. 1:89-94.
Flimlin, G. 1993. Major predators of cultured shellfish. NRAC NRAC Naval Research Advisory Committee
NRAC National Rural Advisory Council
NRAC National Register of Access Consultants (UK)
NRAC Northeastern Regional Aquaculture Center
NRAC Natural Resource Analysis Center Bulletin 180. 6 pp.
Floyd, T. & J. Williams. 2004. Impact of green crab (Carcinus maenas L.) predation on a population of soft-shell clams (Mya arenaria L.) in the southern Gulf of St. Lawrence. J. Shellfish Res. 23:457462.
Gillis, D. J., J. N. MacPherson & T. T. Rattray. 2000. The status of green crab (Carcinus maenas) in Prince Edward Island in 1999. PEI Department of Fisheries and Tourism. Technical Report #225.
Glude, J. B. 1955. The effects of temperature and predators on the abundance of the soft-shell clam, Mya arenaria, in New England New England, name applied to the region comprising six states of the NE United States—Maine, New Hampshire, Vermont, Massachusetts, Rhode Island, and Connecticut. The region is thought to have been so named by Capt. . Trans. Am. Fish. Soc. 84:13-26.
Grosholz, E. D., G.M. Ruiz, C. A. Dean, K.A. Shirley, J.L. Maron & P. G. Conners. 2000. The impacts of a nonindigenous marine predator in a California Bay California bay
See California laurel. . Ecology 81:1206-1224.
Hines, A. H., A. M. Haddon & L. A. Weichert. 1990. Variation in species composition, population dynamics Population dynamics is the study of marginal and long-term changes in the numbers, individual weights and age composition of individuals in one or several populations, and biological and environmental processes influencing those changes. , and foraging impact of blue crabs and epibenthic fish in a subestuary of Chesapeake Bay. Mar. Ecol. Prog. Ser. 67:105-126.
Hughes, R. N. & R. W. Elner. 1979. Tactics of a predator, Carcinus maenas and morphological responses from the prey, Nucella lapillus la·pil·lus
n. pl. la·pil·li
A small, solidified fragment of lava.
[Latin, diminutive of lapis, stone. . J. Anim. Ecol. 48:65-78.
Juanes, F. 1992. Why do decapod decapod (dĕk`əpŏd') (Gr.,=10 feet), name for invertebrate animals of the crustacean order Decapoda (phylum Arthropoda) including the crabs, the lobsters and crayfish, and the true shrimps, all having five pairs of legs. crustaceans prefer small-sized molluscan mol·lus·can also mol·lus·kan
Of or relating to the mollusks.
A mollusk. prey? Mar. Ecol. Prog. Ser. 87:239-249.
Le Calvez, J.-C. 1984. Relations entre la faune annelidienne et un crustace decapode, Carcinus maenas L., dans le bassin maritime de la Rance (Bretagne Nord). Oceanis 10:785-796.
Lindsay, J. A. & N. B. Savage. 1978. Northern New England's threatened soft-shell clam populations. Environ. Manage. 2:443-452.
McDonald, P. S., G. C. Jensen & D. A. Armstrong. 2001. The competitive and predatory impacts of the nonindigenous crab Carcinus maenas (L.) on early benthic phase Dungeness crab Dungeness crab
Edible crab (Cancer magister) found along the Pacific coast from Alaska to lower California, one of the coast's largest and most important commercial crabs. The male is 7–9 in. (18–23 cm) wide and 4–5 in. (10–13 cm) long. Cancer magister MAGISTER. A master, a ruler, one whose learning and position makes him superior to others, thus: one who has attained to a high degree, or eminence, in science and literature, is called a master; as, master of arts. Dana. J. Exp. Mar. Biol. Ecol. 258:39-54.
Menge, B. A. & J. P. Sutherland. 1987. Community regulation: variation in disturbance, competition, and predation in relation to environmental stress and recruitment. Am. Nat. 130:730-757.
Menzel, W. 1989. The biology, fishery and culture of quahaug clams, Mercenaria, p. 201-237. In J. J. Manzi & M. Castagna, editors. Clam mariculture mariculture
marine aquaculture. in North America. New York New York, state, United States
New York, Middle Atlantic state of the United States. It is bordered by Vermont, Massachusetts, Connecticut, and the Atlantic Ocean (E), New Jersey and Pennsylvania (S), Lakes Erie and Ontario and the Canadian province of : Elsevier Science Publisher. 461 pp.
Navarrete, S. A. & J. C. Castilla. 1988. Foraging activities of chilean intertidal crabs Acanthocyclus gayi Milne-Edward et Lucas and A. hassleri Rathbun. J. Exp. Mar. Biol. Ecol. 118:115-136.
O'Neill, S. M., A.M. Sutterlin & D. Aggett. 1983. The effects of size-selective feeding by starfish, Asterias vulgaris, on the production of mussels, Mytilus edulis, cultured on nets. Aquaculture 38:211-220.
Prince Edward Island Aquaculture Alliance, 2000. R & D projects. Available at: http://www.aquaculturepei.com/rdprojectslpriorities.cfm.
Ropes, J. W. 1968. The food habits of five crab species at Pettaquamscutt River The Pettaquamscutt River is a tidal extension of the Mattatuxet River in the U.S. state of Rhode Island. It flows approximately 6 km (4 mi). There are no dams along the river's length. , Rhode Island Rhode Island, island, United States
Rhode Island, island, 15 mi (24 km) long and 5 mi (8 km) wide, S R.I., at the entrance to Narragansett Bay. It is the largest island in the state, with steep cliffs and excellent beaches. . Fish. Bull. 87:197-204.
Rosenthal, H., D. J. Scarratt & M. McInerney-Northcott. 1995. Aquaculture and the environment. In A. D. Boghen, editor. Cold-water aquaculture in Atlantic Canada. The Canadian Institute for Research on Regional Development. pp. 451-500.
Townsend, C. R. & R. N. Hughes. 1981. Maximizing net energy returns from foraging, In C. R. Townsend & P. Calow, editors. Physiological ecology Physiological ecology (animal)
A discipline that combines the study of physiological processes, the functions of living organisms and their parts, with ecological processes that connect the individual organism with population dynamics and community structure. : an evolutionary approach In computer science, an evolutionary approach is an acquisition strategy that defines, develops, produces or acquires, and fields an initial hardware or software increment (or block) of operational capability. to resource use, Blackwell Scientific Publications. Oxford, England: pp. 86-108.
Trussel, G. C. & L. D. Smith. 2000, Induced defenses in response to an invading crab predator: An explanation of historical and geographical phenotypic phe·no·type
a. The observable physical or biochemical characteristics of an organism, as determined by both genetic makeup and environmental influences.
b. change. Proc. Natl. Acad. Sci. USA 97:2123-2127.
Vencile, K.W. 1997. Interactions between naticid gastropods (Euspira spp.) and their bivalve prey (Mya arenaria): effects of clam size, tidal height, and site. MSc. Thesis, University of Maine "UMO" redirects here, but this abbreviation is also used informally to mean the Mozilla Add-ons website, formerly Mozilla Update
Should not be confused with Université du Maine, in Le Mans, France
The University of Maine . 69 pp.
Walton, W. C., C. MacKinnon, L. F. Rodriguez, C. Protor & G. M. Ruiz. 2002. Effect of an invasive crab upon a marine fishery: green crab, Carcinus maenas, predation upon a venerid clam, Katelysia scalarina, in Tasmania (Australia). J. Exp, Mar. Biol. Ecol. 272:171-189.
Welch, W. R, 1968. Changes in abundance of the green crab, Carcinus maenas (L.) in relation to the recent temperature changes. Fish. Bull. 67:337-345.
Whitlow, W. A., N. A. Rice & C. Sweeney. 2003. Native species vulnerability to introduced predators: testing an inducible defence and a refuge from predation. Biol. Inv. 5:23-31.
Williams, A. B. 1984. Shrimps, lobsters, and crabs of the Atlantic coast of the eastern United States, Maine to Florida. Smithsonian Institution Smithsonian Institution, research and education center, at Washington, D.C.; founded 1846 under terms of the will of James Smithson of London, who in 1829 bequeathed his fortune to the United States to create an establishment for the "increase and diffusion of Press. 550 pp.
Zaklan, S. D. & R. Ydenberg. 1997. The body size-burial depth relationship in the infaunal clam Mya arenaria. J. Exp. Mar. Biol. Ecol. 215: 1-17.
GILLES MIRON, (1) * DOMINIQUE AUDET, (1) THOMAS LANDRY (2) AND MIKIO MORIYASU (2)
(1) Departement de biologie, Universite de Moncton, Moncton, Nouveau-Brunswick, Canada E1A 3E9;
(2) Centre des Peches du Golfe, Peches et Oceans Canada, C. P. 5030, Moncton, Nouveau-Brunswick, Canada E1C 9B6
* Corresponding author. E-mail: firstname.lastname@example.org
TABLE 1. Three-way ANOVA examining the effect of predator species, prey species and prey size-classes on mortality rates in single-choice experiments. Data were arcsin transformed. Multiple comparisons were carried out with Tukey multiple comparisons test. Prey species and size-classes are presented in increasing order of mortality rates. Non significant differences among prey species and size-classes are underlined. Source of Variation SS DF MS F P Predator 4.27 1 4.27 0.10 0.922 Prey 14902.88 3 4967.63 11.31 <0.001 Size 7796.27 2 3898.14 8.88 <0.001 Predator x Prey 1586.71 3 528.90 1.21 0.314 Predator x Size 536.10 2 268.05 0.61 0.546 Prey x Size 2700.77 6 450.13 1.03 0.416 Predator x Prey x Size 964.13 6 160.69 0.37 0.898 Error 31614.08 72 439.08 Total 60105.21 95 Multiple comparisons: Prey species Quahaugs Eastern oysters Mussels Soft-shell clams Size-classes (mm) 25-40 15-25 0-15 TABLE 2. Three-way ANOVA examining the effect of predator species, prey species and prey size-classes on mortality rates in multiple-choice experiments. Data were arcsin transformed. The interaction effects were examined using a Tukey test. Prey species and size-classes are presented in increasing order of mortality rates. Non significant differences among prey species and size-classes are underlined. Source of Variation SS DF MS F P Predator 13887.91 1 13887.91 35.33 <0.001 Prey 21094.30 3 7031.43 17.89 <0.001 Size 6879.88 2 3439.94 8.75 <0.001 Predator x Prey 2182.37 3 727.46 1.85 0.146 Predator x Size 4246.70 2 2123.35 5.40 0.007 Prey x Size 8490.98 6 1415.16 3.60 0.004 Predator x Prey x Size 4627.27 6 771.21 1.96 0.082 Error 28303.57 72 393.11 Total 89712.98 95 Multiple comparisons Predator species Prey size-classe(mm) Green crab: 25-40 0-15 15-25 Rock crab: 25-40 0-15 15-25 Prey species Prey size-(m m) Blue mussels: 25-40 0-15 15-25 Eastern oysters: 25-40 0-15 15-25 Soft-shell clams: 25-40 15-25 0-15 Quahaugs: 25-40 15-25 0-15 TABLE 3. Three-way ANOVA examining the effect of predator species, prey species and prey size-classes on mortality rates in single-choice experiments. Data were arcsin transformed. The interaction effects were examined using a Tukey test. Prey size-classes are presented in increasing order of mortality rates. Non significant differences among prey species and size-classes are underlined. Source of Variation SS DF MS F P Predator 1663.25 2 831.63 9.02 <0.001 Prey 1606.60 3 535.54 5.81 0.001 Size 681.40 2 340.70 3.70 0.030 Predator x Prey 2499.09 6 416.52 4.52 0.001 Predator x Size 404.69 4 101.17 1.10 0.365 Prey x Size 2100.85 6 350.14 3.80 0.002 Predator x Prey x Size 4412.41 12 367.70 3.99 <0.001 Error 6639.38 72 92.21 Total 20007.67 108 Multiple comparisons Green crab Prey species Prey size-classes (mm) Blue mussels: 25-40 0-15 15-25 Eastern oysters: 25-40 15-15 0-15 Soft-shell clams: 0-15 15-25 25-40 Quahaugs: 15-15 25-40 0-15 Common seastar Prey species Prey size-classes (mm) Blue mussels: 25-40 15-25 0-15 Eastern oysters: 25-40 15-25 0-15 Soft-shell clams: 25-40 15-25 0-15 Quahaugs: 25-40 15-25 0-15 Moon snail Prey species Prey size-classes (mm) Blue mussels: 25-40 0-15 15-25 Eastern oysters: 25-40 15-15 0-15 Soft-shell clams: 15-25 25-40 0-15 Quahaugs: 25-40 15-25 0-15 TABLE 4. Three-way ANOVA examining the effect of predator species, prey species and prey size-classes on mortality rates in multiple-choice experiments. Data were arcsin transformed. Source of Variation SS DF MS F P Predator 1102.59 2 551.30 2.19 0.119 Prey 193.56 3 64.52 0.26 0.856 Size 39.51 2 19.76 0.08 0.924 Predator x Prey 681.26 6 113.54 0.45 0.841 Predator x Size 292.20 4 73.05 0.29 0.883 Prev x Size 996.10 6 166.02 0.66 0.682 Predator x Prey x Size 2974.92 12 247.91 0.99 0.470 Error 18097.34 72 251.35 Total 24377.47 108