Plasmids in Moss Open Door to New Technique in Biotechnology.
Plasmids, which are DNA DNA: see nucleic acid.
or deoxyribonucleic acid
One of two types of nucleic acid (the other is RNA); a complex organic compound found in all living cells and many viruses. It is the chemical substance of genes. molecules capable of independent replication in cells, have played an important role in gene technology. Researchers from Uppsala University in Sweden have now demonstrated that plasmid-based methods, which had been limited to single-cell organisms such as bacteria and yeasts, can be extended to mosses, opening the door to applications of a number of powerful techniques in plant research.The findings have been published in the distinguished journal Proceedings of the National Academy of Sciences of the USA (PNAS).Professor Hans Ronne's research team at the Department of Medical Biochemistry and Microbiology at Uppsala University is working with the diminutive Physcomitrella moss which is widely used in research because its genes are easier to "knock out" than those of other plants. Previous work has also shown that DNA introduced into Physcomitrella cells is capable of self-replication through an as yet uncharacterised process.The new study, which was led by Dr. Eva MurE n. 1. A wall.
v. t. 1. To inclose in walls; to wall; to immure; to shut up.
imp. & p. p. os>
The five kings are mured in a cave.
- John. x. (Heading). [umlaut]n and Ph.D. student Anders Nilsson, showed that plasmids introduced into moss cells can be rescued back to bacteria without affecting the plasmids' original structures, provided that certain conditions are met. Up to now, various kinds of rearrangements have sharply limited the use of plasmids in animal- and plant-cell research."Our work with plasmids in moss suggests that it will be possible to use powerful methods such as gene cloning by complementation Complementation (genetics)
The complementary action of different genetic factors. The term usually implies two homologous chromosomes or chromosome sets, each defective because of mutation and unable by itself to promote the normal development or metabolism of and overexpression directly in plant cells without recourse to single-cell organisms like bacteria or yeasts," Professor Hans Ronne said."This, in turn, may simplify basic and applied research and biotechnology involving plants," the professor added.
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