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Pathological study of sheep lymph nodes in Tabriz abattoir.


Pathological studies of lymph nodes are of paramount importance while giving diagnosis of certain diseases. Perusal of available literature revealed meager information, hence present communication Reports the gross, and microscopic lesions of different regional lymph nodes of indigenous cattle and their etiological relationship. This study is the first report in Iran.

Material and methods

Lymph nodes from sheeps were collected from the slaughter houses which were located Tabriz area (center of East Azerbaijan province). Four different regional lymph nodes (popliteal, External iliac, Prescapular and Internal iliac) were selected for the investigation during the period of October, 2010 to September, 2010. The lymph nodes exhibiting any gross pathological lesions were cut into two halves by a sharp sterile knife. One half of each lymph node was put into 10% neutral buffered formalin and processed routinely for histopathological examination. In this study for samples staining from Hemotoxylin and eosin were used.

Results and Discussion

All results of this study consist of gross examination and microscopic examination in following tables has been shown.

Among 1600 lymph nodes of sheeps examined for any gross pathological lesions, only 115 were found to be abnormal. According to results of gross examination lesions in popliteal, External iliac, Prescapular and Internal iliac respectively with 20%, 26.96%, 42.6% and 10.44%were distinguished. In fact in gross examination maximum lesions in Prescapular lymph nodes were observed. By attention to results of microscopic findings Pigmented lymph nodes were found with highest frequency.

Study on lesions of lymph nodes of cattle or sheeps is very rare and our present article is very similar to one study by Maity and et al. Maity et al. in 2000 on slaughtered cattle of Calcutta and Rural areas worked on Prescapular, Supramammary, Bronchial and Mesenteric and maximum of lesions in Mesenteric lymph nodes and Minimum of lesions in lymph nodes belong to prescapular lymph nodes were reported [4]. Grossly, enlarged lymph nodes were found to be edematous, turgid, somewhat soft and cut surface buldged slightly. Most of lymph nodes were pale in color. Histopathologically, enlarged lymph nodes usually showed a reactive follicular hyperplasia. The lymphoid follicles were increased in number and size. Haemorrhagic lymph nodes were also swollen, dark red or bright red in color. Hyperemia was a constant feature. The cut surface of the nodes were deep red or bright red in colour. Histopathologically, haemorrhagic lymph nodes showed large number of erythrocytes in lymph sinuses. Congestion and extravasation of erythrocytes were found in some vessels. Large numbers of erythrocytes were found in the lymph sinuses of the lymph nodes. The gross and histopathological features of haemorrhagic lymph nodes were in accordance with the earliest findings [1,2,3]. Pigmented lymph nodes were dark black or brown in colour, dry and hard in consistency. The cut sections showed a black center of varying shape surrounded by a lighter zone. Histopathologically, the Pigmented lymph nodes revealed the presence of pigments confined to the trabeculae of medullary part, in the form of black grains in the cytoplasm of enlarged reticular cells. These lesions were in accordance with the earlier descriptions [2,5,6]. In present study the most type of pigmentation in lymph nodes was of Anthracosis and hemosiderosis.In cases of lymphadenitis mononuclear cells and eosinophil were observed. In pyogenic lymph nodes, a few small or large abscesses were found and some of these lymph nodes had yellowish-white foci. Pyogenic lymph nodes also revealed the presence of pus in the medullary region encapsulated by connective tissue. In some cases, accumulated pus cells had replaced the normal histological structure of the lymph nodes. In end must say this study is first report in Iran and in fact as base for other studies in present region for determine the agents of these lesions.


[1.] Jennings, A.R., 1970. Animal pathology, 1st edn. Balliere Tindall&Casell, London, 10-14.

[2.] Jubb, K.V.F., P.C. Kenndy and N. Palmer, 1985. Pathology of Domestic animals, 3rd edn, Acaademic press, London, 3: 181-188.

[3.] Kumar, G.S., A.K. Sharma, P.K.R. Iyer and M.C. Prasad, 1998. Tuberculosis in crossbred diarycattle. Indian journal of veterinary pathology, 22: 11-15.

[4.] Maity, S.B., D. Pratima, D. Ratna, T.L. Som, 2000. Pathology of lymph nodes in cattle. Indian journal of veterinary pathology, 24: 32-34.

[5.] Nieberle and Cohrs, 1996. Text book of the special pathological anatomy of Domestic animals. 1st edn. Pergamon press, London, 73-93.

[6.] Sharma, A.K., P.R. Vanamayya and N.S. Parihar, 1985. Tuberculosis in cattle: a retrospective study based on necropsy. Indian Journal of Veterinary Pathology, 9: 14-18.

(1) Javadi. Afshin, (2) Safarmashaei. Saeid

(1) Department of food hygiene, Tabriz branch, Islamic Azad University, Tabriz, Iran.

(2) Tabriz branch, Islamic Azad University, Young Researchers Club.

Corresponding Author

Javadi Afshin, Department of food hygiene, Tabriz branch, Islamic Azad University, Tabriz, Iran.


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Table 1: Result of gross examination of sheep lymph nodes.

Regional lymph nodes    Number    %

popliteal               23        20
External iliac          31        26.96
Prescapular             49        42.6
Internal iliac          12        10.44
Total                   115       100

Table 2: Frequency of different types of pathological lesions in
different regional lymph nodes in cattle.

--                Enlarged    Haemorrhagic    Pigmented    Pyogenic

popliteal         2           1               15           3
External iliac    --          8               15           --
Prescapular       10          5               9            2
Internal iliac    --          5               5            --
Total             12          19              44           5

--                caseous          Hyperemia    Total

popliteal         --               2            23
External iliac    --               8            31
Prescapular       14               9            49
Internal iliac    --               2            12
Total             14               21           115
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Title Annotation:Original Article
Author:Afshin, Javadi; Saeid, Safarmashaei
Publication:Advances in Environmental Biology
Article Type:Report
Geographic Code:7IRAN
Date:May 1, 2011
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