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Pathological study of cattle lymph nodes.

Introduction

Lymph nodes are part of the lymphatic system, which carries waste products and other materials away from the cells in body. These materials are carried to the lymph nodes in a colorless fluid called lymph. Lymph nodes filter this fluid before it returns to the blood stream. Lymph nodes also store white blood cells (called lymphocytes) that help fight infection. Pathological studies of lymph nodes are of paramount importance while giving diagnosis of certain diseases. Perusal of available literature revealed meager information, hence present communication Reports the gross, and microscopic lesions of different regional lymph nodes of indigenous cattle and their etiological relationship. This study is the first report in Iran.

Material and methods

Lymph nodes from cattle were collected from the slaughter houses which were located Tabriz area (center of East Azerbaijan province). Seven different regional lymph nodes (Retropharyngeal, Mesenteric, Supramammary, Bronchial, Portal, Prescapular and Internal iliac) were selected for the investigation during the period of October, 2010 to September, 2010. The lymph nodes exhibiting any gross pathological lesions were cut into two halves by a sharp sterile knife. One half of each lymph node was put into 10% neutral buffered formalin and processed routinely for histopathological examination. In this study for samples staining from Hemotoxylin and eosin were used.

Results and Discussion

All results of this study consist of gross examination and microscopic examination in following tables has been shown.

Among 1200 lymph nodes of cattle examined for any gross pathological lesions, only 189 were found to be abnormal. According to results of gross examination lesions in Bronchial, Mesenteric, Portal, Prescapular, Supramammary, Internal iliac and Retropharyngeal respectively with 19.57, 16.93, 15.34, 13.75, 12.56, 12.02 and 10.92 were distinguished. In fact in gross examination maximum lesions in Bronchial lymph nodes were observed. By attention to results of microscopic findings enlarged lymph nodes were found with highest frequency because this type of alteration was the earliest finding of any type of antigenic stimulation.

Study on lesions of lymph nodes of cattle or sheeps is very rare and our present article is very similar to one study by Maity and et al. Maity et al. in 2000 on slaughtered cattle of Calcutta and Rural areas worked on Prescapular, Supramammary, Bronchial and Mesenteric and maximum of lesions in Mesenteric lymph nodes and Minimum of lesions in lymph nodes belong to prescapular lymph nodes were reported [4]. Grossly, enlarged lymph nodes were found to be edematous, turgid, somewhat soft and cut surface buldged slightly. Most of lymph nodes were pale in color. Histopathologically, enlarged lymph nodes usually showed a reactive follicular hyperplasia. The lymphoid follicles were increased in number and size. Haemorrhagic lymph nodes were also swollen, dark red or bright red in color. Hyperemia was a constant feature. The cut surface of the nodes were deep red or bright red in colour. Histopathologically, haemorrhagic lymph nodes showed large number of erythrocytes in lymph sinuses. Congestion and extravasation of erythrocytes were found in some vessels. Large numbers of erythrocytes were found in the lymph sinuses of the lymph nodes. The gross and histopathological features of haemorrhagic lymph nodes were in accordance with the earliest findings [1,2,3]. Caseated lymph nodes were enlarged, hard in consistency, indurated and contained greyish-white caseous mass. Sections of the few lymph nodes showed presence of calcified mass which were surrounded by fibrous capsules. Microscopically, caseated lymph nodes depicted early caseation intermingled with lymphoblasts and lymphocytes. Presence of macrophages, epithelioid cells and langhans type of giant cells were predominantly found. The epithelioid and giant cells were the products of reticuloendothelial cell proliferation suggesting the phagocytic activity against the foreign bodies, lesions of the caseated lymph nodes corroborated with the earlier reports [3,5,6]. Pigmented lymph nodes were dark black or brown in colour, dry and hard in consistency. The cut sections showed a black center of varying shape surrounded by a lighter zone. Histopathologically, the Pigmented lymph nodes revealed the presence of pigments confined to the trabeculae of medullary part, in the form of black grains in the cytoplasm of enlarged reticular cells. These lesions were in accordance with the earlier descriptions [2, 5]. In present study the most type of pigmentation in lymph nodes was of Anthracosis and hemosiderosis. In cases of Eosinophilic lymphadenitis mononuclear cells and eosinophil were observed. In end must say this study is first report in, in fact as base for other studies in present region for determine the agents of these lesions.

Reference

[1.] Jennings, A.R., 1970. Animal pathology, 1st edn. Balliere Tindall&Casell, London, 10-14.

[2.] Jubb, K.V.F., P.C. Kenndy and N. Palmer, 1985. Pathology of Domestic animals, 3rd edn, Acaademic press, London, 3: 181-188.

[3.] Kumar, G.S., A.K. Sharma, P.K.R. Iyer and M.C. Prasad, 1998. Tuberculosis in crossbred diary cattle. Indian Journal of Veterinary Pathology, 22: 11-15.

[4.] Maity, S.B., D. Pratima, D. Ratna, T.L. Som, 2000. Pathology of lymph nodes in cattle. Indian Journal of Veterinary Pathology, 24: 32-34.

[5.] Nieberle and Cohrs, 1996. Text book of the special pathological anatomy of Domestic animals. 1st edn. Pergamon press, London, 73-93.

[6.] Sharma, A.K., P.R. Vanamayya and N.S. Parihar, 1985. Tuberculosis in cattle: a retrospective study based on necropsy. Indian Journal of Veterinary Pathology, 9: 14-18.

(1) Javadi Afshin, (2) Safarmashaei Saeid

(1) Department of food hygiene, Tabriz branch, Islamic Azad University, Tabriz, Iran.

(2) Tabriz branch, Islamic Azad University, Young Researchers Club.

Corresponding Author

Javadi Afshin, Department of food hygiene, Tabriz branch, Islamic Azad University, Tabriz, Iran.

E-mail:Afshinjavadi@yahoo.com Javadi@iaut.ac.ir

Tel: 00989141165059
Table 1: Result of gross examination of cattle lymph nodes

Regional lymph nodes    Number    %

Retropharyngeal         20        10.92
Mesenteric              32        16.93
Supramammary            23        12.56
Bronchial               37        19.57
Portal                  29        15.34
Prescapular             26        13.75
Internal iliac          22        12.02
Total                   189       100

Table 2: Frequency of different types of pathological lesions in
different regional lymph nodes in cattle

--                 Enlarged    Haemorrhagic    Pigmented

Retropharyngeal    11          8               --
Mesenteric         14          11              8
Supramammary       14          8               3
Bronchial          15          6               18
Portal             15          13              --
Prescapular        18          11              3
Internal iliac     16          13              1
Total              103         70              33

                               Eosinophilic
--                 Caseated    lymphadenitis    Total

Retropharyngeal    9           --               28
Mesenteric         --          --               33
Supramammary       --          --               5
Bronchial          6           4                49
Portal             --          15               43
Prescapular        --          --               33
Internal iliac     --          --               30
Total              15          19               --
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Title Annotation:Original Article
Author:Afshin, Javadi; Saeid, Safarmashaei
Publication:Advances in Environmental Biology
Article Type:Report
Geographic Code:7IRAN
Date:May 1, 2011
Words:1063
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