Outsmarting olfaction: the next generation of mosquito repellents.
The world's most dangerous animal weighs about two milligrams and pursues its human prey at speeds of barely a mile per hour. Surprised? Don't be. The dubious honor belongs to the lowly mosquito--a fragile creature whose bite infects millions with lethal diseases, such as malaria, dengue dengue
or breakbone fever or dandy fever
Infectious, disabling mosquito-borne fever. Other symptoms include extreme joint pain and stiffness, intense pain behind the eyes, a return of fever after brief pause, and a characteristic rash. , and West Nile West Nile may refer to:
Shortcomings may also be:
Today, the need for more effective repellents is increasingly urgent, experts say. According to according to
1. As stated or indicated by; on the authority of: according to historians.
2. In keeping with: according to instructions.
3. the World Health Organization, global climate change is expanding mosquitoes' range, heightening the risk of disease for millions of additional people. The Centers for Disease Control and Prevention Centers for Disease Control and Prevention (CDC), agency of the U.S. Public Health Service since 1973, with headquarters in Atlanta; it was established in 1946 as the Communicable Disease Center. notes that dengue and West Nile virus West Nile virus, microorganism and the infection resulting from it, which typically produces no symptoms or a flulike condition. The virus is a flavivirus and is related to a number of viruses that cause encephalitis. are both moving from developing countries towards the United States United States, officially United States of America, republic (2005 est. pop. 295,734,000), 3,539,227 sq mi (9,166,598 sq km), North America. The United States is the world's third largest country in population and the fourth largest country in area. , where concerns over mosquito exposure are rising. Malaria--which by various estimates kills between 1 million and 3 million people worldwide each year--is also a growing problem in many regions. This is in part because Plasmodium plasmodium, name for a stage in the life cycle of a slime mold. Also, Plasmodium is the name given to the genus of the protozoan parasite that causes malaria. , the mosquito-borne parasite that causes malaria, is fast becoming resistant to existing treatments, such as chloroquine chloroquine /chlo·ro·quine/ (klor´o-kwin) an antiamebic and anti-inflammatory used in the treatment of malaria, giardiasis, extraintestinal amebiasis, lupus erythematosus, and rheumatoid arthritis; used also as the hydrochloride and .
Many experts believe that better repellents could help to control mosquito-borne diseases. These next-generation repellents must be effective against anopheline anopheline
pertaining to the anopheles genus of mosquitoes. mosquitoes that carry malaria. They should also be cheap and nontoxic, and should last long enough to protect humans as they sleep, when they are most vulnerable.
Where will these repellents come from? The answers, scientists increasingly say, will be found in genomic research. According to this view, knowledge of the genes and proteins that mosquitoes rely on to sense their environment could lead to new repellents that directly interfere with the insects' ability to detect human beings.
A Sense of the Future
Today, genomic information about mosquitoes is accruing rapidly. Scientists with the International Anopheles Genome Project genome project 1 The Human Genome Project, see there 2. A general term for a coordinated research initiative for mapping and sequencing the genome of any organism , a consortium based at the Pasteur Institute The Pasteur Institute (French: Institut Pasteur) is a French non-profit private foundation dedicated to the study of biology, microorganisms, diseases and vaccines. in Paris, have already decoded the genome of An. gambiae, the dominant malaria vector in Africa. Another genome, for Aedes aegypti, a vector for both yellow fever yellow fever, acute infectious disease endemic in tropical Africa and many areas of South America. Epidemics have extended into subtropical and temperate regions during warm seasons. and dengue, is now being sequenced with funding from the National Institute of Allergy and Infectious Diseases. Using these data sets, scientists are identifying the genes that control mosquito sensory systems, including the olfactory system.
Mosquitoes rely on smell to guide them towards mates, food, and of course, sources of blood meals. The process is highly specific. For example, only female mosquitoes are attracted to blood sources, which they require as nourishment for their eggs. (Mosquitoes' main nutriment nutriment /nu·tri·ment/ (noo´tri-mint) nutrient (2).
1. A source of nourishment; food.
2. An agent that promotes growth or development. comes from other sources, mainly plant nectar.) Olfaction is also species-specific. An. gambiae, for instance, prefers to bite humans. "You could be in a room full of cows, and that mosquito will find you and bite you," says Laurence Zwiebel, an associate professor of biology at Vanderbilt University. Because this species has evolved to target humans, it will stay indoors where it can get to them more easily, he adds.
To better understand the molecular biology molecular biology, scientific study of the molecular basis of life processes, including cellular respiration, excretion, and reproduction. The term molecular biology was coined in 1938 by Warren Weaver, then director of the natural sciences program at the Rockefeller of mosquito olfaction, consider the following scenario: You're asleep in the summertime, in a warm room with an open window. Your body and the bacteria that reside on your skin are giving off a molecular cloud of human-specific odorants. These molecules drift through the air and soon reach a mosquito perched on the wall. In an instant, odorant-binding proteins (OBPs) located on the creature's antennae bind the molecules and transport them towards receptors located on the surfaces of the olfactory olfactory /ol·fac·to·ry/ (ol-fak´ter-e) pertaining to the sense of smell.
Of, relating to, or contributing to the sense of smell. neurons. The odorant-receptor linkage activates a cue in the mosquito's nervous system, which alerts the insect to your presence. Moments later, the mosquito lands on your unprotected shoulder and begins to feed.
OBPs are now widely viewed as one of the most likely targets for next-generation repellents. Compounds that interfere with these proteins could block mosquitoes' ability to smell and thus detect humans. "This really is a unique approach," says Leslie Vosshall, head of the Laboratory of Neurogenetics neu·ro·ge·net·ics
The study of genetic factors that contribute to development of neurological disorders. and Behavior at Rockefeller University. "Since we now know quite a bit about the basic workings of insect olfactory systems, we can focus our search for new repellents that interrupt proteins known to be important for smell. We're optimistic that this rational approach to repellent design will uncover new compounds that are safer and more effective than those that are currently available."
Targeting Olfactory Proteins
Vosshall has found that the function of a wide range of odorant odorant /odor·ant/ (o´der-int) any substance capable of stimulating the sense of smell.
odorant receptors in insects depends on a single type of protein "coreceptor" that facilitates the binding of the OBP-odorant complex to the receptor. Working with fruit flies, she has shown that mutations in this protein, which in fruit flies is called Or83b, knocks out the insect's sense of smell altogether. The protein, she says, is highly conserved across many different species; in mosquitoes, it now goes by the name GPRor7 (until recently, it was known as AgOR7). In recent studies, Vosshall generated a strain of fruit flies that lacked the Or83b gene and hence the ability to smell. This finding, remarkable in its own right, was rendered even more so by a subsequent discovery: when she replaced the missing Or83b gene with the GPRor7 gene obtained from mosquitoes, the fruit flies' olfactory system was restored to a normal state. This means GPRor7 substituted for Or83b, even though mosquitoes and fruit flies diverged more than 250 million years ago.
Based on these findings, Vosshall suggests that GPRor7 is a plausible target for a mosquito repellent. Knock out this protein, she says, and a mosquito will be unable to smell anything--humans included. Her findings are published in the 22 February 2005 issue of Current Biology.
Zwiebel and his colleagues are taking a different tack. Instead of targeting a single protein that regulates mosquitoes' entire sense of smell, he's homing in on the olfactory proteins that bind human odorants only. In the 15 January 2004 issue of Nature, Zwiebel published results showing that one olfactory receptor in An. gambiae--a protein known as Orl--preferentially binds 4-methylphenol, one of roughly 300 compounds found in human sweat. Moreover, the Orl receptor was found only in female mosquitoes--further evidence of the intricate specificity in the insect's olfactory machinery.
Today, Zwiebel and his colleagues are striving to identify other human-specific odorants with the goal of creating repellents that inactivate in·ac·ti·vate
1. To render nonfunctional.
2. To make quiescent.
in·acti·va groups of these targets simultaneously. This approach lessens the likelihood that mosquitoes will develop resistance to the repellents, he says. "It's almost impossible for resistance mutations to appear in four to five receptors concurrently, and they would not make their way into the mosquito population," he explains. "What we really want is a toolbox of behaviorally disruptive olfactory compounds," he adds. "This would allow us to fine-tune repellent blends for specific geographic regions, where mosquito populations and olfactory mechanisms might vary."
The future repellents mark an additional departure because they could be incorporated into time-release systems that put active ingredients into the air. Thus, there would be no need to apply the repellents to the body; instead, they would protect a living space in its entirety. This is important for those mosquitoes--An. gambiae in particular--that prefer indoor environments and sleeping humans.
Scratching the Surface
Experts agree that no new repellent will necessarily be a panacea for mosquito control. Andrew Spielman, a professor of tropical public health at the Harvard University School of Public Health and author of the book Mosquito: A Natural History of Our Most Persistent and Deadly Foe, suggests that all repellents pose a fundamental dilemma: mosquitoes deterred from one protected person will simply flock in greater numbers to another who is unprotected. "What drives the force of disease transmission is the biting rate," he explains. "If half the people are being bitten by all the mosquitoes, then those people and any mosquitoes that feed on them will wind up being terrifically infected." But Spielman acknowledges that repellents can be very useful--"I'm just not sure about who specifically is going to benefit from them," he says.
When posed with this question, Zwiebel responds that health officials must take steps to ensure that no one is left unprotected. "That's a challenge we need to accept," he says. "We need to make sure these products are universally available and economically affordable."
In the end, new repellents will be just part of a broader strategy to control mosquitoes, a strategy that Spielman says must also incorporate better environmental management, housing improvements, and greater use of insecticide-treated bed nets. Meanwhile, it's hard to say which type of rationally designed repellent is likely to emerge first. Will it target one protein, as Vosshall suggests it could, or will it target several, as Zwiebel says it must? "At the end of the day," Zwiebel says, "everyone is going forward with the best of intentions, and we just have to see what comes out."
Jones WD, Nguyen TA, Kloss B, Lee KJ, Vosshall LB. 2005. Functional conservation of an insect odorant receptor gene across 250 million years of evolution. Curt Biol 15(4):R119-R121.
Justice RW, Biessmann H, Walter MF, Dimitratos SD, Woods DF. 2003. Genomics spawns novel approaches to mosquito control. BioEssays 25:1011-1020.
Spielman A, D'Antonio M. 2001. Mosquito: A Natural History of Our Most Persistent and Deadly Foe. New York New York, state, United States
New York, Middle Atlantic state of the United States. It is bordered by Vermont, Massachusetts, Connecticut, and the Atlantic Ocean (E), New Jersey and Pennsylvania (S), Lakes Erie and Ontario and the Canadian province of , NY: Hyperion Books.