Optimization Times for Measuring Stress-Induced Immunodepression at the Secreted IL2 and Secreted IL4 Level of the Immune Response.Immunodepression im·mu·no·de·pres·sion
an absence or deficient supply of the components of either humoral or cellular immunity, or both. See also agammaglobulinemia, hypogammaglobulinemia, immunodeficiency. has been reported as stress-induced. The detection of this immuno-depression is possible through the measurement of the levels of secreted interleukin-2 (IL2) and interleukin-4 (IL4) found in peripheral blood mononuclear cells (PBMC PBMC Peripheral Blood Mononuclear Cell ). These cells were collected from the blood by Ficoll-Paque centrifugation and resuspended in an isotonic isotonic /iso·ton·ic/ (-ton´ik)
1. denoting a solution in which body cells can be bathed without net flow of water across the semipermeable cell membrane.
2. medium, Hanks Balanced Salt Solution (HBSS HBSS Hank's Balanced Salt Solution
HBSS Hanks' Buffered Salt Solution
HBSS High Band Sub-System
HBSS Host-Based Security System
HBSS Hill Billy Snap Shooter (Joe Clark photography book) ). The measurement of the secreted IL2 and secreted IL4 was performed by a BioE bead flow cytometric method. Capture beads coated with antiIL2 or antiIL4 were added to the supernatant to bind with the IL2 and IL4 present in the supernatant. With the addition of a labeled reporter body, the intensity of the fluorescence was plotted on a standard curve. This enabled the calculation (pg) of the secreted cytosine cytosine (sī`tōsēn'), organic base of the pyrimidine family. It was isolated from the nucleic acid of calf thymus tissue in 1894. . The optimum time for the binding of antiIL2 to the IL2 and antiIL4 to the IL4 was determined to be twenty hours, but these results require further experimentation due to the small number of controls. After this, additional studies can be performed as to whether immunodepression is stress-induced.
Salem Wolk South Carolina Governor's School for Science and Mathematics