Nonsusceptibility of primate cells to Taura syndrome virus.
Taura syndrome virus Taura syndrome virus
cause of severe losses in juvenile prawns Penaeus vanammei. (TSV TSV - tab-separated values ), a pathogen of penaeid shrimp and member of the family Dicistreviridae, was recently reported to have the ability to infect primate cells. We independently retested this hypothesis. Three lines of primate cells FRhK-4, MA-104, and BGMK, which are highly susceptible to infection by human picornaviruses, were challenged with TSV. Viral replication was assayed by real-time reverse transcription-polymerase chain reaction using cell media samples collected on days 0, 4, and 7 postchallenge. By day 7, genome copy numbers had decreased 25%-99%. No cytopathic effect was observed after 7 days. An in situ hybridization in situ hybridization A method for localizing a sequence of DNA, mRNA, or protein in a cell or tissue; the use of a DNA or RNA probe to detect a cDNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured assay, with gene probes specific for detection of TSV, was negative for TSV in challenged cells. The infectivity of residual virus in the cell culture media at day 7 was confirmed by bioassay Bioassay
A method for the quantitation of the effects on a biological system by its exposure to a substance, as well as the quantitation of the concentration of a substance by some observable effect on a biological system. using TSV-free indicator shrimp (Litopenaeus vannamei). TSV did not infect the primate cells tested, and no evidence of zoonotic potential zoonotic potential
The potential for animal infections to be transmissible to humans. was found.
The general assumption is that the viral agents that cause disease in penaeid shrimp do not infect vertebrates. Supporting this assumption is the absence of documented cases of any shrimp virus causing disease in any animal species other than crustaceans. In a recent article, Taura syndrome virus (TSV), exclusively a pathogen of penaeid shrimp, was attributed a zoonotic potential because of its reported ability to infect cultured human and monkey cells (1). Aside from the food safety issues raised by this report, we were very interested in confirming those results because of the practical value of this new option for growing TSV in vitro in vitro /in vi·tro/ (in ve´tro) [L.] within a glass; observable in a test tube; in an artificial environment.
In an artificial environment outside a living organism. . To date, TSV (or any other of the known viruses of the penaeid shrimp) has not yet been successfully cultured in any invertebrate invertebrate (ĭn'vûr`təbrət, –brāt'), any animal lacking a backbone. The invertebrates include the tunicates and lancelets of phylum Chordata, as well as all animal phyla other than Chordata. or vertebrate cell-culture system. Hence, if viable, the use of primate cells for propagation of TSV would prove to be an important advancement in the study of TSV, and perhaps of other crustacean crustacean (krŭstā`shən), primarily aquatic arthropod of the subphylum Crustacea. Most of the 44,000 crustacean species are marine, but there are many freshwater forms. viruses. While the experiment reported in this study did not include all of the cell lines used by Audelo-del-Valle et al. (1), namely human rhabdomyosarcoma rhabdomyosarcoma /rhab·do·myo·sar·co·ma/ (mi?o-sahr-ko´mah) a highly malignant tumor of striated muscle derived from primitive mesenchymal cells. (RD), human larynx carcinoma (Hep-2), and Buffalo green monkey kidney (BGMK), the three cell lines that we used are also routinely used for virus isolation and diagnosis of diseases caused by human enteroviruses Enteroviruses
Viruses which live in the gastrointestinal tract. Coxsackie viruses, viruses that cause hand-foot-mouth disease, are an enterovirus.
Mentioned in: Hand-Foot-and-Mouth Disease that belong to the family Picornaviridae (2-7). TSV, is a member of the family Dici,stroviridae (closely related to Picornaviridae), genus Cripavirus (8,9). Other than TSV, which only infects penaeid shrimp, members of the genus Cripavirus are known to infect only insects (9). We report the results obtained after performing an experiment to test the hypothesis proposed by Audelo-del-Valle et al. (1).
Materials and Methods
Source of TSV
Rather than using TSV-infected shrimp originated from shrimp farms as a source, TSV infection was induced under laboratory conditions by injecting specific pathogen free specific pathogen free
a term applied to animals reared for experimentation or to commence new herds or flocks of disease-free animals; abbreviated SPF. Animals usually obtained as for axenic animals but are then placed into a nonsterile environment in which they become infected (SPF (1) (Stateful Packet Firewall) See stateful inspection.
(2) (Sender Policy Framework) An e-mail authentication system that verifies that the message came from an authorized mail server. ) shrimp (10) Litopenaeus vannamei with purified TSV reference isolate Hawaii-94 (11). The use of SPF shrimp ensured that no contamination with other viral pathogens would interfere with the experiment. Hemolymph hemolymph /he·mo·lymph/ (he´mo-limf?)
1. blood and lymph.
2. the bloodlike fluid of those invertebrates having open blood-vascular systems.
n. and hepatopancreas The hepatopancreas is an organ of the digestive tract of arthropods, gastropods and fish. It provides the functions which in mammals are provided separately by the liver and pancreas. were obtained from moribund shrimp during the acute phase of Taura syndrome and used to prepare the viral inocula.
Preparation of Inocula
Hemolymph was drawn from a moribund shrimp with acute-phase Taura syndrome and the hepatopancreas was excised by using aseptic aseptic /asep·tic/ (-tik) free from infection or septic material.
Of, relating to, or characterized by asepsis. technique. The hemolymph was diluted 1:10 with Eagle's balanced salts minimum essential medium (EMEM), without fetal bovine sera (FBS FBS
fasting blood sugar
FBS Fasting blood sugar. See Fasting glucose. ), and filtered through a syringe filter of 0.22-[micro]m pore size. The hepatopancreas was homogenized ho·mog·e·nize
v. ho·mog·e·nized, ho·mog·e·niz·ing, ho·mog·e·niz·es
1. To make homogeneous.
a. To reduce to particles and disperse throughout a fluid.
b. in 10 mL of EMEM without FBS, centrifuged at 125 x g for 2 min to eliminate coarse material and the supernatant supernatant /su·per·na·tant/ (-na´tant) the liquid lying above a layer of precipitated insoluble material.
the liquid lying above a layer of precipitated insoluble material. filtered with a syringe filter off 0.22-[micro]m pore size. Samples of hemolymph and hepatopancreas from SPF shrimp were processed in identical manner and used as a negative control.
The lines and cell cultures used were African green monkey kidney (BGMK), Monkey Rhesus female kidney embryonic (FRhK-4), and Monkey African green kidney (MA-104). Other than the report by Audelo-del-Valle et al. (1), TSV culture or CPE (Customer Premises Equipment) Communications equipment that resides on the customer's premises.
CPE - Customer Premises Equipment has not been reported in any invertebrate or vertebrate cell line. Hence, no positive control for TSV-induced CPE in cell culture was included in this study.
Each of four 75-[cm.sup.2] flasks of confluent con·flu·ent
1. Flowing together; blended into one.
2. Merging or running together so as to form a mass, as sores in a rash. monolayers of each cell line (BGMK, FRhK-4, and MA-104) was injected with 0.1 mL of either of the four inocula: 1) hemolymph from shrimp with acute-phase Taura syndrome, 2) hepatopancreas from shrimp with acute-phase Taura syndrome, 3) hemolymph from SPF shrimp, or 4) hepatopancreas from SPF shrimp. Alter injection, the standard volume (15 mL) of fresh EMEM with 2% FBS was added without removing the inoculum inoculum /in·oc·u·lum/ (-ok´u-lum) pl. inoc´ula material used in inoculation.
n. pl. . The cells were incubated at 37[degrees]C and monitored once a day for 7 days for cytopathic effect (CPE). As an additional negative control, one flask of each of the three cell lines was left untreated but monitored once a day alongside the TSV-injected flasks.
As an additional test to determine if a productive TSV infection occurred, representative samples of cells at day 7 were collected with a sterile pipette pipette /pi·pette/ (pi-pet´) [Fr.]
1. a glass or transparent plastic tube used in measuring or transferring small quantities of liquid or gas.
2. to dispense by means of a pipette. and pelleted at 130 x g. The pellet of cells was fixed in Davidson's AFA AFA
In currencies, this is the abbreviation for the Afghanistan Afghani.
The currency market, also known as the Foreign Exchange market, is the largest financial market in the world, with a daily average volume of over US $1 trillion. (alcohol, formaldehyde, and acetic acid) fixative fixative /fix·a·tive/ (fik´sit-iv) an agent used in preserving a histological or pathological specimen so as to maintain the normal structure of its constituent elements.
adj. and processed by using conventional techniques for paraffin embedding and sectioning. Paraffin sections were subjected to in situ hybridization with a mixture of two gene probes, P15 and Q1, specific for detection of TSV (12), according to protocols published elsewhere (13,14).
RNA RNA: see nucleic acid.
in full ribonucleic acid
One of the two main types of nucleic acid (the other being DNA), which functions in cellular protein synthesis in all living cells and replaces DNA as the carrier of genetic Extraction
A total of 0.2 mL from each of the original inocula (inocula prepared from the hemolymph and hepatopancreas of infected and noninfected shrimp) and 0.2 mL of cell culture media from each of the three different cell line cultures collected at days 0, 4, and 7 postexposure, were subjected to RNA extractions using a High Pure RNA tissue kit (Roche Molecular Biochemicals, Indianapolis, IN), according to the manufacturer's recommendations. The concentration of extracted RNA was estimated by measuring optical density, O[D.sub.260nm], with an Eppendorf spectrophotometer spectrophotometer, instrument for measuring and comparing the intensities of common spectral lines in the spectra of two different sources of light. See photometry; spectroscope; spectrum. .
Real-Time TSV RT-PCR RT-PCR
reverse transcriptase-polymerase chain reaction. See PCR1.
The real time TSV RT-PCR assays were performed using an ABI Abi (ā`bī) [short for Abijah], in the Bible, King Hezekiah's mother.
(Application Binary Interface) A specification for a specific hardware platform combined with the operating system. GeneAmp 5700 with TaqMan One-Step RT-PCR master mixture (Applied Biosystems, Foster City, CA). The reaction mixture contained no more than 10 ng of extracted RNA, with each primer at a concentration of 0.3 [micro]mol/L, and the TaqMan probe at a concentration of 0.1 [micro]mol/L in a final volume of 25 [micro]L. The cycling consisted of 30 min at 48[degrees]C for reverse transcription and 10 min at 95[degrees]C, followed by 40 cycles of 95[degrees]C for 15 s, and 60[degrees]C for 1 min. The data acquisition and analysis were carried out with GeneAmp 5700 Sequence Detector Software (Applied Biosystems). The real-time RT-PCR primers and TaqMan probe for the detection of TSV had been previously designed from ORF1 region of the TSV genomic sequence (15). Serial dilutions from a previously constructed TSV plasmid were used to determine a standard linear relationship for quantification with a correlation of the serial dilutions >0.99.
Confirmation of TSV Infectivity
To confirm the infectivity of the virus, a 6-day bioassay was performed by injecting groups of four to six SPF indicator shrimp (L. vannamei) with approximately 200 [micro]L of either of the following: 1) inoculum prepared from hemolymph of infected shrimp; 2) inoculum prepared from hemolymph of noninfected shrimp; 3) cell media collected at day 7 from TSV-challenged cell culture flasks; or 4) culture media collected at day 7 from SPF shrimp tissue-treated cell culture flasks (negative control). The shrimp were monitored once a day for signs of disease. Moribund shrimp were collected when observed, preserved in Davidson's fixative and transferred into 70% ethanol after 24 h (14,16). All surviving shrimp at termination of the bioassay (day 6) were preserved in the same manner. Shrimp tissue samples were processed according to conventional techniques for paraffin embedding and sectioning (16). Paraffin sections were stained with Mayer-Bennett's hematoxylin/eosin-phloxin and subjected to histologic evaluation to determine the presence of TSV diagnostic lesions. Selected specimens were subjected to a confirmatory assay by in situ hybridization (ISH ISH In Situ Hybridization
ISH Isolated Systolic Hypertension
ISH Irish Sport Horse
ISH Intermediate System Hello
ISH International Society of Hypnosis
ISH Information Super Highway
ISH International Superhits (Green Day album) ) with a mixture of two gene probes, P15 and Q1, specific for detection of TSV (12-14).
Cytopathic Effect (CPE)
No CPE was observed in any of the three cell lines injected with TSV infected hemolymph, TSV infected hepatopancreas, SPF shrimp hemolymph, or SPF shrimp hepatopancreas (Figure 1). The BGMK cell line showed normal fibroblastic structure throughout the 7-day period of exposure to the different treatments. The BGMK monolayer mon·o·lay·er
1. A film or layer one molecule thick formed at the interface between water and either oil or air by a substance such as a partially esterified fatty acid that contains both hydrophobic and hydrophilic groups in the same remained confluent with no evidence of cell detachment or lysis lysis /ly·sis/ (li´sis)
1. destruction or decomposition, as of a cell or other substance, under influence of a specific agent.
2. mobilization of an organ by division of restraining adhesions.
3. . Similarly, the FRhK-4 and the MA-104 cell lines retained their typical epithelial structure for the 7-day period after exposure to TSV, with confluent monolayers and no evidence of cell detachment or lysis.
[FIGURE 1 OMITTED]
Approximately 1.3 x [10.sup.5] to 2.7 x [10.sup.6] viral copies/[micro]L were detected at day 0 in the tissue cell flasks exposed to TSV-infected hemolymph. In the case of the tissue cell flasks exposed to YSV-infected hepatopancreas, [approximately equal to]1.2 x [10.sup.4] viral copies/[micro]L were detected (Table). TSV was not detected by real time RT-PCR in the inoculum prepared from SPF shrimp hemolymph and hepatopancreas, nor in the tissue cell culture flasks exposed to these inocula.
At day 7, real time RT-PCR showed a decrease of 25% to 99% of the TSV genome copy number in the tissue cell culture flasks exposed to TSV (Figure 2), which suggests that no viral replication had occurred but that some residual virus remained from the inoculum.
[FIGURE 2 OMITTED]
Samples of cell-culture media from tissue culture flasks injected with TSV-infected hemolymph were collected at day 7 and used to inject SPF indicator shrimp L. vannamei to determine the infectivity of the residual virus. Moribund shrimp from these bioassays were examined by conventional hematoxylin/eosin-phloxin histology and by in situ hybridization with the gene probes specific for detection of TSV. TSV infection was diagnosed in all of the moribund shrimp, which indicates that at 7 days after injection, the tissue culture media contained sufficient residual TSV to produce infections in challenged shrimp (Figure 3). Paraffin sections from known TSV-infected and noninfected shrimp were used as ISH positive and negative controls, respectively (results not shown).
[FIGURE 3 OMITTED]
During this study, a relationship was observed between the concentration of TSV in the inocula prepared from day 7 tissue culture media (from cells exposed to TSV-infected hemolymph) and the severity of TSV infection in the challenged SPF indicator shrimp. The shrimp that had been injected with tissue cell culture media with the highest TSV concentration ([approximately equal to]2.5 x [10.sup.4] viral copies/[micro]L) developed an acute (overt) infection within 3 days postchallenge, whereas shrimp injected with tissue cell culture media with the lowest viral concentration ([approximately equal to]2.3 x [10.sup.3] viral copies/[micro]L) developed only a subacute (covert) infection. Both overt and covert infections were confirmed by histologic analysis and by ISH with gene probes specific for detection of TSV (Figure 4).
[FIGURE 4 OMITTED]
Additional ISH Test
As an additional test to further confirm the absence of viral replication, the accumulation within the cells, or both, a sample of cells at day 7 was obtained from the BGMK cell line and subjected to ISH with TSV-specific gene probes. The BGMK cell line was selected for this assay because, among all three lines, it had the highest initial (day 0) concentration of viral particles and a 2 log reduction at day 7, suggesting either degradation or internalization Internalization
A decision by a brokerage to fill an order with the firm's own inventory of stock.
When a brokerage receives an order they have numerous choices as to how it should be filled. of the virus. The ISH assay gave negative results (Figure 5), which indicates degradation as the more likely explanation for the reduction in virus content of the cell media. As in previous ISH assays, paraffin sections from known TSV-infected and noninfected shrimp were used as ISH-positive and -negative controls, respectively (results not shown).
[FIGURE 5 OMITTED]
The real-time RT-PCR results (Table) show that the number of TSV genome copies in the cell culture media did not increase for any of the three cell lines challenged with TSV. While differences were observed in the estimated number of viral copies in each flask at day 7, the number of viral copies present was from one to two logs less than that of the day 0 values, which were determined immediately after cells were injected. The apparent plateau of TSV counts at day 7, regardless of the concentration of viral particles in each flask at day 0, may have been due to a protective effect of the cell-culture media, specifically the fetal bovine serum Fetal bovine serum ( or foetal bovine serum) is serum taken from the fetuses of cows. Fetal Bovine Serum (or FBS) is the most widely used serum in the culturing of cells. In some papers the expression foetal calf serum is used. (FBS). This protective effect of FBS on viruses has been documented by other researchers (17-20). Studies on viral transport media for the preservation of virus viability have concluded that the best transport media for specimens at risk of being delayed by long transit times and exposed to significant temperature variations en route, are those that contain 2% FBS (i.e., CVM-Copan Diagnostics, Corana, CA, and M4-Multi-Microbe, Micro Test, Inc., Snellville, GA).
To further confirm the absence of viral replication or accumulation within the cells, a sample of cells at day 7 was obtained from the BGMK cell line and subjected to ISH with TSV-specific gene probes. The absence of reaction to the TSV probes and the absence of CPE indicate that viral replication did not occur in the BGMK cells and that if any viral particles were internalized, they were degraded.
The relationship observed between the concentration of TSV in the inocula prepared from day 7 tissue culture media (from cells exposed to TSV-infected hemolymph) and the severity of TSV infection in the challenged SPF indicator shrimp agree with the results obtained during a previous study in the authors' laboratory, in which it was concluded that a minimum concentration of [approximately equal to] 1.0 x [10.sup.4] viral copies/[micro]L is necessary to induce an acute infection (21). in this case, those shrimp that had been injected with tissue cell culture media with the highest TSV concentration ([approximately equal to]2.5 x [10.sup.4] viral copies/[micro]L) developed an acute (overt) infection within 3 days postchallenge, whereas shrimp injected with tissue cell culture media with the lowest viral concentration ([approximately equal to]2.3 x [10.sup.3] viral copies/[micro]L) developed only a subacute (covert) infection.
BGMK, FRhK-4, and MA-104 cell lines are often used for isolation and research purposes for enteroviral meningitis (3), hepatitis A virus Noun 1. hepatitis A virus - the virus causing hepatitis A
enterovirus - any of a group of picornaviruses that infect the gastrointestinal tract and can spread to other areas (especially the nervous system) (7), polioviruses, coxsackie A, and coxsackie B (22) because of their marked susceptibility to infection by these members of the Picornaviridae. When exposed to any of these agents, these cell lines develop conspicuous CPE within [approximately equal to]5 days (3,7,22,23). However, no CPE in any of the three cell lines (BGMK, FRhK-4, and MA-104) challenged with TSV was observed in this experiment, even at day 7 postinjection. These results contradict those of Audelo-del-Valle et al. (1), who reported the development of CPE within 19 23 hours. The average incubation time required for CPE development (induced by enteroviruses) in human or monkey cells at 37[degrees]C is 5 days, although detection time may be reduced to [approximately equal to]3 days by use of the shell vial method (23). Shorter incubation times of <24 hours for CPE development could be more suggestive of a toxicity problem rather than of virus induced CPE.
As mentioned above, SPF shrimp (L. vannamei) were used to amplify a reference strain of TSV to prepare the inocula. We used SPF shrimp for three reasons. First, pond-reared or wild shrimp may harbor human or other mammalian picornaviruses. Shrimp and other decapod decapod (dĕk`əpŏd') (Gr.,=10 feet), name for invertebrate animals of the crustacean order Decapoda (phylum Arthropoda) including the crabs, the lobsters and crayfish, and the true shrimps, all having five pairs of legs. crustaceans have been shown to internalize internalize
To send a customer order from a brokerage firm to the firm's own specialist or market maker. Internalizing an order allows a broker to share in the profit (spread between the bid and ask) of executing the order. and passively carry certain fish viruses (24-26) and human enteroviruses (27, C. Gerba, unpub. data). Hence, wild or pond-reared shrimp may be passive carriers of human or other mammalian pricornaviruses or other viruses which could produce CPE in studies such as that reported by Audelo-del-Valle et al. (1). Second, by using a commercially available line of SPF shrimp, the experiment can be standardized; therefore, other researchers can repeat or confirm the present study. Third, the SPF shrimp used are produced in closed biosecure systems with controlled water sources, which preclude chance contamination of the stocks with human or other animal viruses.
BGMK cells were the only cell type in common between our study and that of Audelo-del-Valle et al (1), who also used RD and Hep-2 however, BGMK, FRhK-4, and MA-104 cells were selected for use in our study because these cell types have a marked susceptibility to infection by members of Picornaviridae (28), which makes them as adequate as RD or Hep-2 cells for determining the possible infectivity of TSV to primate cells. We conclude that TSV did not infect the primate cells challenged with TSV in our study.
The lack of CPE in any of the three different cell lines tested, the negative ISH results with TSV specific gene probes assay of TSV challenged BGMK cells, and multi-log reduction in TSV number in the cell-culture media as determined by real time RT-PCR indicate that TSV did not replicate in the primate cell lines used in our study. That TSV infection had occurred in SPF indicator shrimp after injection with media collected from day 7 cell culture flasks indicates that sufficient residual TSV remained in the media to infect the challenged shrimp and to cause acute disease or subacute disease as a function of relative concentration of residual TSV present.
Funding for this research was provided by the USDA USDA,
n.pr See United States Department of Agriculture. CSREES CSREES Cooperative State Research, Education, and Extension Service (USDA) Marine Shrimp Fanning Program, grant number 2002-38808-01345, and a special grant from the National Fishery Institute.
Dr. Pantoja is associate research professor at the Aquaculture aquaculture, the raising and harvesting of fresh- and saltwater plants and animals. The most economically important form of aquaculture is fish farming, an industry that accounts for an ever increasing share of world fisheries production. Pathology Laboratory, University of Arizona (body, education) University of Arizona - The University was founded in 1885 as a Land Grant institution with a three-fold mission of teaching, research and public service. (an OIE OIE Office International des Épizooties (French: International Office of Epizootics; Paris)
OIE Oficina Internacional de Epizootias (Spanish: World Organization for Animal Health) reference laboratory for Taura syndrome and other diseases of aquatic organisms), where he forms part of a team that provides disease diagnostic services to the international shrimp farming industry and conducts research on new and emerging diseases of shrimp.
Table. Mean TSV quantification in tissue cell culture media (a,b) Mean viral quantification (viral copies/ [micro]L) postexposure Cell type Source of inoculum Inoculum type Day 0 BGMK Hemolymph TSV-infected 2.7 x [10.sup.6] SPF (c) 0 Hepatopancreas TSV-infected 1.2 x [10.sup.4] SPF 0 FrhK-4 Hemolymph TSV-infected 1.3 x [10.sup.5] SPF 0 Hepatopancreas TSV-infected 1.3 x [10.sup.4] SPF 0 MA-104 Hemolymph TSV-infected 1.5 x [10.sup.5] SPF 0 Hepatopancreas TSV-infected 1.2 x [10.sup.4] SPF 0 Mean viral quantification (viral copies/[micro]L) postexposure Day 4 Day 7 Cell type 2.4 x [10.sup.5] 2.5 x [10.sup.4] 0 0 BGMK 5.0 x [10.sup.3] 5.0 x [10.sup.3] 0 0 1.9 x [10.sup.4] 4.7 x [10.sup.3] 0 0 FrhK-4 8.4 x [10.sup.3] 3.7 x [10.sup.3] 0 0 3.1 x [10.sup.4] 2.3 x [10.sup.3] 0 0 MA-104 1.3 x [10.sup.4] 9.2 x [10.sup.3] 0 0 (a) Estimated by real time reverse transcription-polymerase chain reaction at different intervals postexposure. (b) BGMK, Buffalo green monkey kidney; FrhK-4, monkey Rhesus female kidney embryonic, MA-104, monkey African green kidney; TSV; Taura syndrome virus; SPF, specific pathogen free. (c) Inoculum originated from SPF penaeid shrimp.
(1.) Audelo-del-Valle J, Clement-Mellado O, Magana-Hernandez A, Flisser A, Montiel-Aguirre F, Briseno-Garcia B. Infection of cultured human and monkey cell lines with extract of penaeid shrimp infected with Taura syndrome virus. Emerg Infect Dis. 2003;9:265-6.
(2.) Agbalika F, Hatermann P, Foliguet JM. Trypsin-treated Ma-104: a sensitive cell line for isolating enteric viruses from environmental samples. Appl Environ Microbiol. 1984;47:378 80.
(3.) Buck GE, Wiesemann M, Stewart L. Comparison of mixed cell culture containing genetically engineered BGMK and CaCo-2 cells (Suer E-Mix) with RT-PCR and conventional cell culture for the diagnosis of enterovirus enterovirus /en·tero·vi·rus/ (en´ter-o-vi?rus) any virus of the genus Enterovirus. enterovi´ral
Enterovirus /En·tero·vi·rus/ (en´ter-o-vi?rus meningitis. J Clin Virol. 2002;25:S13-8.
(4.) Kok TW, Pryor T, Payne L. Comparison of rhabdomyosarcoma, Buffalo green money kidney epithelial, A549 (human lung epithelial) cells and human embryonic lung fibroblasts Fibroblasts
A type of cell found in connective tissue; produces collagen.
Mentioned in: Skin Grafting for isolation of enteroviruses from clinical samples. J Clin Virol. 1998;11:61-5.
(5.) Landry M, Garner R, Ferguson. Rapid enterovirus RNA detection in clinical specimens by using nucleic acid sequence based amplification. J Clin Microbiol. 2003;41:346 50.
(6.) Otero JR, Folgueira L, Trallero G, Prieto C, Maldonado S. Babiano MJ, et al. A-549 is a suitable cell line for primary isolation of cox-sackie B viruses. J Med Virol. 2001;65:534-6.
(7.) Sanchez G, Pinto RM, Vanaclocha H, Bosch A. Molecular characterization of hepatitis A virus isolates from a transcontinental shellfish-borne outbreak. J Clin Microbiol. 2002;40:4148-55.
(8.) Mayo MA. ICTV ICTV International Committee on Taxonomy of Viruses
ICTV Independent Community Television Alliance at the Paris ICV ICV Integrity Check Value (IETF Authentication Header for IPV6 and V4)
ICV Iniciativa per Catalunya Verds
ICV Infantry Carrier Vehicle
ICV Infantry Combat Vehicle : results of the plenary session and binomial binomial (bī'nō`mēəl), polynomial expression (see polynomial) containing two terms, for example, x+y. The binomial theorem, or binomial formula, gives the expansion of the nth power of a binomial (x+ ballot. Arch Virol. 2002;147:1655-63.
(9.) Mari J, Poulos BT, Lightner DV, Bonami J-R. Shrimp Taura syndrome virus: genomic characterization and similarity with members of the genus Cricket paralysis-like viruses. J Gen Virol. 2002;83:915-26.
(10.) Wyban JA, Swingle Swin´gle
v. i. 1. To dangle; to wave hanging.
2. To swing for pleasure.
v. t. 1. To clean, as flax, by beating it with a swingle, so as to separate the coarse parts and the woody substance from it; to scutch. JS, Sweeney JN, Pruder GD. Development and commercial performance of high health shrimp using specific pathogen free (SPF) broodstock Penaetts vannamei. In: Wyban J, editor. Proceedings of the Special Session on Shrimp Farming. Baton Rouge (LA): World Aquaculture Society; 1992. p. 254-9.
(11.) Nunan LM, Poulos BT, Lightner DV. Reverse transcription polymerase chain reaction “RT-PCR” redirects here. For real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction or kinetic polymerase chain reaction, see real-time polymerase chain reaction. (RT-PCR) used for the detection of Taura syndrome virus in experimentally infected shrimp. Dis Aquat Organ. 1998:34:87-91.
(12.) Mari J. Bonami J-R, Lightner DV. Taura syndrome of penaeid shrimp: cloning of viral genome fragments and development of specific gene probes. Dis Aquat Organ. 1998;33:11-7.
(13.) Hasson KW, Lightner, DV, Mohney LM, Redman RM, Poulos BT, Mart J, et al. The geographic distribution of Taura syndrome virus (TSV) in the Americas: determination by histopathology his·to·pa·thol·o·gy
The science concerned with the cytologic and histologic structure of abnormal or diseased tissue.
The study of diseased tissues at a minute (microscopic) level. and in situ hybridization using TSV-specific cDNA probes. Aquaculture. 1999;17:13-26.
(14.) Lightner DV. A handbook of shrimp pathology and diagnostic procedures for diseases of cultured penaeid shrimp. Baton Rouge (LA): World Aquaculture Society; 1996.
(15.) Tang KT, Lightner DV. Quantitation of Taura syndrome virus by real-time RT-PCR with TaqMan assay. J Virol Methods. 2004;115:109-14.
(16.) A handbook of normal penaeid shrimp histology. Baton Rouge (LA): World Aquaculture Society; 1998.
(17.) Johnson FB. Transport of viral specimens. Clin Microbiol Rev. 1990:3:120-31.
(18.) Jensen C, Johnson FB. Comparison of various transport media for viability maintenance of herpes simplex virus Herpes simplex virus
A virus that can cause fever and blistering on the skin, mucous membranes, or genitalia.
Mentioned in: Conjunctivitis
herpes simplex virus , respiratory syncytial virus respiratory syncytial virus (sĭnsĭsh`əl): see cold, common. and adenovirus adenovirus
Any of a group of spheroidal viruses, made up of DNA wrapped in a protein coat, that cause sore throat and fever in humans, hepatitis in dogs, and several diseases in fowl, mice, cattle, pigs, and monkeys. . Diagn Microbiol Infect Dis. 1994; 19:137-42.
(19.) Josephson SL. An update on the collection and transport of specimens for viral culture. Clinical Microbiology Newsletter. 1997;57-64.
(20.) Dunn JJ, Billetdeaux E, Skodack-Jones L, Cfarroll KC. Evaluation of three Copan viral transport systems for the recovery of cultivable, clinical virus isolates. Diagn Microbiol Infect Dis. 2003:45:191-7.
(21.) Poulos BT, Lightner DV. Analysis of samples from Litopenaeus' vannamei during chronic phase Taura syndromevirus (TSV) infection. In: Book of abstracts, Aquaculture America 2003, new frontiers in aquaculture. Baton Rouge (LA): World Aquaculture Society; 200Y p. 237.
(22.) Weng KT, Pryor T, Payne L. Comparison of rhabdomyosarcoma, Buffalo green monkey kidney epithelial, A549 (human lung epithelial) cells and human embryonic lung fibroblasts for isolation of enteroviruses from clinical samples. J Clin Virol. 1998;11:61-5.
(23.) Huang YT, Yam P, Yan H, Sun Y. Engineered BGMK ceils for sensitive and rapid detection of enteroviruses. J Clin Microbiol. 2002;40:366-71.
(24.) Bovo G, Ceschia G, Giorgetti G, Vanelli M. Isolation of an IPN-like virus from adult Kuruma shrimp (Penaeus japonicus). Bulletin of the European Association of Fish Pathologists. 1984;4(2):21.
(25.) Halder M, Ahne W. Freshwater crayfish crayfish or crawfish, freshwater crustacean smaller than but structurally very similar to its marine relative the lobster, and found in ponds and streams in most parts of the world except Africa. Crayfish grow some 3 to 4 in. (7.6–10. Astacus astacus--a vector for infectious pancreatic necrosis virus (IPNV IPNV Infectious Pancreatic Necrosis Virus
IPNV Input Parameter Not Valid (Alcatel) ). Dis Aquat Organ. 1988:4:205-9.
(26.) Lu Y, Cesar E, Nadala CB, Brock JA, Loh PC. A new virus isolate from infectious hypodermal and hematopoietic necrosis Infectious Hypodermal and Hematopoietic Necrosis (IHHN) is a viral disease of penaeid shrimp that causes mass mortality (up to 90%) among the Western blue shrimp (Penaeus stylirostris) and severe deformations in the Pacific white shrimp (P. vannamei). virus (IHHNV IHHNV Infectious Hypodermal and Hematopoietic Necrosis Virus )-infected penaeid shrimps. J Virol Methods. 1991;31:189-96.
(27.) Hejkal TW, Gerba CHR CHR
canine hypoxic rhabdomyolysis. Uptake and survival of enteric viruses in the Blue crab, Callinectes sapidus. Appl Environ Microbiol. 1981:41:207-11.
(28.) Sair AI, D'Souza DH, Jaykus LA. Human enteric viruses as causes of foodborne disease. Comprehensive Reviews in Food Science and Food Safety The Comprehensive Reviews in Food Science and Food Safety (usually abbreviated as CRFSFS) is an American online peer-reviewed scientific journal published by the Institute of Food Technologists (IFT) in Chicago, Illinois. . 2002:1:73-89.
Carlos R. Pantoja, * Solangel A. Navarro, * Jaime Naranjo, * Donald V. Lighter, * and Charles P. Gerba * * University of Arizona, Tucson, Arizona, USA
Address for correspondence: C.R. Pantoja, University of Arizona, OIE Reference Laboratory for Taura Syndrome, 1117 E. Lowell St., Building 90, Room 114, Tucson, Arizona 85721, USA: fax: 520-621-4899: email: firstname.lastname@example.org