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Chair: Charlotte Brunner, University of Southern Mississippi

Vicechair: Jeffrey Lotz, University of Southern Mississippi


Ship Isle

8:40 BOTTOM SCATTERING VARIABILITY DURING THE 1996 PANAMA CITY, FLORIDA Panama City is a city located along U.S. Highway 98 in Bay County, Florida. It is the largest city between Pensacola, Florida and Tallahassee, Florida. It is the larger (population wise) of two principal cities of the Panama City-Lynn Haven, Florida Metropolitan Statistical Area. , HIGH-FREQUENCY ACOUSTIC EXPERIMENT

Marcia A. Wilson [1][*], Jerald W. Caruthers [1], Ralph Goodman [2] and Steve Stanic [1], (1.) Naval Research Laboratory Noun 1. Naval Research Laboratory - the United States Navy's defense laboratory that conducts basic and applied research for the Navy in a variety of scientific and technical disciplines
, Stennis Space Center, MS 39529 and (2.) Naval Research Laboratory on IPA IPA - International Phonetic Alphabet  from Pennsylvania State University Pennsylvania State University, main campus at University Park, State College; land-grant and state supported; coeducational; chartered 1855, opened 1859 as Farmers' High School. , State College, PA 16804

An experiment was performed in shallow water near Panama City, Florida to study spatial variability of high-frequency bottom reverberation. A towed body with two transducer arrays installed at 15 and 40 degrees from horizontal was tested. The active transducer emitted a lms acoustic pulse every tenth of a second as the towed body moved over the relatively uniform sand bottom. Frequency rotated among 4 to 7 frequencies between 75 and 375 kHz. Reverberation envelopes for each frequency were plotted to show the changes in amplitude and arrival time for a series of pulses interacting with the bottom along the track of the towed body. An integrated reverberation level for each pulse was obtained. Several statistical analysis methods were used to determine whether changes in the reverberation probability density function Probability density function

The function that describes the change of certain realizations for a continuous random variable.
 were sufficient to indicate a difference in seafloor characteristics encountered. Analysis based on the chi-squared test was able to quantify differences between segments of data along a given tra ck.


Daniel N. Fox [*] and Germana Peggion, Naval Research Laboratory, Stennis Space Center, MS 39529 and University of Southern Mississippi, Stennis Space Center, MS 39529

A scalable, rapidly relocatable ocean analysis and forecast system has been developed to provide accurate estimates of the acoustic environment. The system is built from reusable software modules, which facilitates adding or modifying capabilities, and runs well on a wide spectrum of computer hardware, from low-end workstations to supercomputers. In situ measured ocean profiles of temperature and salinity are optimally interpolated interpolated /in·ter·po·lat·ed/ (in-ter´po-la?ted) inserted between other elements or parts.  into a novel synthetic ocean environment which is generated using remotely sensed data and linear regressions derived from 100 years of measured profiles. This analysis is then used to initialize To start anew, which typically involves clearing all or some part of memory or disk.  a robust, relocatable version of the Princeton Ocean Model The Princeton Ocean Model (POM) is a community general circulation numerical (computer) ocean model that can be used to simulate and predict oceanic currents, temperatures, salinities and other water properties. Dynalysis of Princeton, a private company organized by H. , including tides. The system has been found to be much more accurate than other commonly used ocean climatologies and will be demonstrated in several areas including the northern Gulf of Mexico Noun 1. Gulf of Mexico - an arm of the Atlantic to the south of the United States and to the east of Mexico
Golfo de Mexico

Atlantic, Atlantic Ocean - the 2nd largest ocean; separates North and South America on the west from Europe and Africa on the east


William H. Bumett [*] and Vladimir M Kamenkovich, Stennis Space Center, MS 39529-5001 and University of Southern Mississippi, Stennis Space Center, MS 39529

A high-resolution, regional, four-open port, nonlinear, barotropic ocean model (2D POM) is used to show that a pressure difference between the Pacific and Indian Ocean is not the dominant factor determining the total transport of the Indonesian throughflow. Two types of experiments were performed. In Experiment 1, the normal and tangential velocities at the ports are prescribed. The total transports through these ports are taken from analyses of historical observations, and the total inflow and outflow balanced to ensure mass conservation. Experiment 1's steady state results were used as boundary conditions for Experiment 2 where sea surface elevations and tangential velocities are specified at the open ports. To study the influence of the pressure head, the sea-surface elevation found in Experiment 1 was perturbed per·turb  
tr.v. per·turbed, per·turb·ing, per·turbs
1. To disturb greatly; make uneasy or anxious.

2. To throw into great confusion.

 by a constant value at the open ports. A series of experiments were performed and the relative importance of the different terms in the momentum equations were analyzed both within and outside the equatorial zone.

9:40 HIGH PERFORMANCE VISUALIZATION OF METOC METOC meteorological and oceanographic (US DoD)
METOC Mission Essential Meteorological and Oceanographic Center

George W. Heburn, Naval Meteorology and Oceanography, Stennis Space Center, MS 39529

Historically, graphical representations have been used to convey complex meteorological and oceanographic A term used to convey all meteorological (weather) and oceanographic (physical oceanography) factors as provided by Service components. These factors include the whole range of atmospheric and oceanographic phenomena, from the sub-bottom of the earth's oceans up to the space environment  (METOC) information to the end user. Initially, this was in the form of hand drawn analyses of an iso-surface of some METOC parameter, i.e., pressure, temperature, etc. and sent to remote users via facsimile broadcast. Today, with the wide spread use of computers and the Internet, the primary conveyance of METOC is in the form of computer generated analyses of iso-surfaces and 2D and 3D grid fields of NIETOC parameters. As the desktop graphics workstations become more powerful and immersive display devices become more prevalent the opportunity exists to make better use of scientific visualization techniques to provide METOC information to the Warfighter. The Naval Meteorology and Oceanography Command (NMOC NMOC Non-Methane Organic Compound
NMOC Naval Meteorology and Oceanography Command (US Navy)
NMOC Nippon Mitsubishi Oil Corporation
NMOC National Meteorological Operations Centre (Australia) 
) in partnership with Mississippi State University Mississippi State University, at Mississippi State, near Starkville; land-grant and state supported; coeducational; chartered 1878 as an agricultural and mechanical college, opened 1880. From 1932 to 1958 it was known as Mississippi State College.  (MSU MSU Michigan State University
MSU Mississippi State University
MSU Montana State University
MSU Minnesota State University
MSU Morehead State University (Kentycky)
MSU Montclair State University
), Jackson State University Jackson State University, often abridged as Jackson State or by its initials JSU is a historically black university located in Jackson, Mississippi founded in 1877.  (JSU JSU Jacksonville State University
JSU Jackson State University (Jackson, MS, USA)
JSU Jewish Student Union
), and the Center for Higher Learning/University of Southern Mississippi (CHL/USM), has embarked on a High Performa nce Visualization Center Initiative (HPVCI). The HPVCI will create the infrastructure for ...a center without walls, in which the researchers can perform their research without regard to geographical location interaction with colleagues, accessing instrumentation, sharing data and computational resources, and accessing information in digital libraries. This will initially include efforts in the following: (1) collaborative scientific visualization; (2) the management/manipulation of very large geophysical and simulation data sets; (3) adaptive computation for battlefield tactics; and (4) electronic classroom, distance learning and educational outreach. The ultimate goal of this research program is to develop better visualization techniques to provide the Warfighter with the METOC information needed to exploit the natural environmental condition to his advantage.

10:00 Break

n. pl. hy·drog·ra·phies
1. The scientific description and analysis of the physical conditions, boundaries, flow, and related characteristics of the earth's surface waters.


Denis Denis, king of Portugal: see Diniz.  A. Wiesenburg [*], Donald G. Redalje, and Andre Godin, University of Southern Mississippi, Stennis Space Center, MS 39529

A new graduate degree program in Hydrographic Science has recently been established by The University of Southern Mississippi (USM USM
1. United States Mail

2. United States Mint

USM n abbr (= United States Mint) → US-Münzanstalt (= United States Mail) → US-Postbehörde
) Department of Marine Science. Along with the U.S. Navy, USM now offers, one-year non-thesis Master of Science degree in Hydrographic Science. Presently, there are no accredited accredited

recognition by an appropriate authority that the performance of a particular institution has satisfied a prestated set of criteria.

accredited herds
cattle herds which have achieved a low level of reactors to, e.g.
 academic programs in Hydrographic Science, within the United States, that are certified at the Category A level by the FIG/IHO International Advisory Board. The USM curriculum has been designed to meet requirements of the FIG/IHO Category A standards for academic proficiency in hydrographic surveying. The curriculum will be presented to the FIG/IHO International Advisory Board for certification in April 2000. The USM Department of Marine Science is located at the Stennis Space Center that is also the home of the U.S. Naval Oceanographic Office (NA VOCEANO), the Naval Research Laboratory-Stennis Space Center (NRL-SSC NRL-SSC Naval Research Laboratory, Stennis Space Center ) and the NOAA NOAA
National Oceanic and Atmospheric Administration

Noun 1. NOAA - an agency in the Department of Commerce that maps the oceans and conserves their living resources; predicts changes to the earth's environment;
 National Data Buoy Center The National Data Buoy Center (NDBC), is a part of the National Oceanic and Atmospheric Administration's (NOAA) National Weather Service (NWS). NDBC designs, develops, operates, and maintains a network of data collecting buoys and coastal stations. . Department of Marine Science faculty members, as well as other USM faculty with related knowledge and expertise, will actively participate in the delivery of this new graduate degree program in Hydrographic Science. Expertise from nearby industry and governmental agencies (e.g. NRL-SSC, NAVOCEANO NAVOCEANO Naval Oceanographic Office ) are also available to provide additional support to the program. The unique aggregation of hydrographers, oceanographers, and facilities at the Stennis Space Center provides an ideal location for this new program.

10:40 ONTOGENETIC on·to·ge·net·ic
Of or relating to ontogeny.

Kenneth R. Camp [1][*], Patricia M. Biesiot [1], and Jeffrey M. Lotz [2], (1.) University of Southern Mississippi, Hattiesburg, MS 39406-5018 and (2.) Gulf Coast Research Laboratory, Institute of Marine Sciences The Institute of Marine Sciences (IMS) focuses on marine science-related education and research. IMS was founded in 1975 on the Erdemli Campus at METU (Middle East Technical University) in Erdemli / Mersin. , Ocean Springs, MS 39566-7000

Changes in proximate analysis (protein and lipid), lipid classes, and fatty acid composition were monitored during early development of red snapper Lutjanus campechanus. Ova, embryos, and larvae from day 0 to 17 days post-hatch were sampled. Protein was the major constituent at 49-78% of the ash-free dry mass (AFDM AFDM Ash Free Dry Mass
AFDM Medium Auxiliary Floating Dry Dock (Non Self-Propelled)
AFDM Advanced Fluid Dynamics Model
) and increased consistently, in mass and proportion, between the embryo and 17 day old stages. Total lipids were highest in ova and embryos at 34% AFDM, decreased markedly to 12% AFDM by day 3 due to yolk absorption, and increased to 16% AFDM by 17 days post-hatch because of exogenous feeding. Proportions of major lipid classes changed during development. Sterol esters (SE) and triacylglycerols (TAG) occurred in ova but were almost completely depleted by end of the yolk sac stage (3 days post-hatch). By day 17, SE and TAG stores were replenished. Phosphatidyl choline choline: see vitamin.

Organic compound related to vitamins in its activity. It is important in metabolism as a component of the lipids that make up cell membranes and of acetylcholine.
 and phosphatidyl ethanolamine ethanolamine /eth·a·nol·amine/ (eth?ah-nol´ah-men) monoethanolamine.

ethanolamine oleate
 remained at high levels throughout development, presumably pre·sum·a·ble  
That can be presumed or taken for granted; reasonable as a supposition: presumable causes of the disaster.
 conserved because of their roles as membran e lipids. The dominant fatty acids were docosahexaenoic acid (22:6[omega]3), palmitic acid (16:0), oleic acid (18:1[omega]9), eicosapentaenoic acid (20:5[omega]3), and linolenic acid (18:3[omega]3) which comprised, respectively, 35-40 mol%, 12-17 mol%, 8-12 mol%, 4-7%, 0-7% of the fatty acid pool. Partial funding was provided through NMFS/DOC grant number NA86FL0476.

11:00 PHYTOPLANKTON phytoplankton

Flora of freely floating, often minute organisms that drift with water currents. Like land vegetation, phytoplankton uses carbon dioxide, releases oxygen, and converts minerals to a form animals can use.

Karie E. Holtermann [*] and Donald G. Redalje, University of Southern Mississippi, Stennis Space Center, MS 39522

As part of an environmental quality study of the St. Louis Bay, Mississippi, samples were taken for High Performance Liquid Chromatography High-performance liquid chromatography (HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry. It is also sometimes referred to as high-pressure liquid chromatography.  (HPLC HPLC high-performance liquid chromatography.


high performance liquid chromatography.

HPLC High-performance liquid chromatography Lab instrumentation A highly sensitive analytic method in which analytes are placed
) phytoplankton pigment analysis. Samples were taken at 9 stations during incoming and outgoing tides over the course of 9 months. Pigment analysis using HPLC followed the procedure of (Wright et al. 1991). A model using signature pigments as indicators of phytoplankton species was used to determine trends. In coastal and estuarine es·tu·a·rine  
1. Of, relating to, or found in an estuary.

2. Geology Formed or deposited in an estuary.

Adj. 1. estuarine - of or relating to or found in estuaries
 environments it is expected that larger phytoplankton such as diatoms diatoms

a series of unicellular algae, microscopic in size, with cell walls containing silica. Members of the family Diatomaceae. Their remains accumulate as geological deposits and are mined. See diatomaceous earth.
 or dinoflagellates dinoflagellates

minute aquatic protozoa; they produce red pigment and toxins which are taken up by shellfish without apparent ill effect, but the toxin is not metabolized and the shellfish may poison animals if eaten.
 will dominate due to their ability to utilize higher concentrations of nutrients found there. Smaller phytoplankton will dominate in oceanic environments where nutrients are not as abundant. Analysis of two stations, one representing an estuary environment inside St. Louis Bay and one representing the Mississippi Sound environment were compared. Diatoms were more abundant in the Mississippi Sound while dinoflagellates dominated in St. Louis Bay as show n by the presence of indicator pigments.


Amber F. Garber [*], Kenneth C. Stuck, and Walter D. Grater, University of Southern Mississippi, Institute of Marine Sciences, Gulf Coast Research Laboratory, Ocean Springs, MS 39564

The ultimate goal of a marine finfish finfish

fish with fins, that is teleosts, elasmobranches, holocephalids, agnathids and cephalochordates; also a fish marketer's term used to include that section of marketable fish which is neither shellfish nor molluscs.
 stock enhancement program is the release of hatchery-reared fish that successfully reproduce and interbreed interbreed

to breed between animal or plant species, breeds, families.
 with the receiving native populations. Since generational contributions of released fish cannot be estimated by physical tagging, a reliable genetic tag must be identified and employed. As part of an ongoing stock enhancement program for red snapper, Lutjanus campechanus, in the northern Gulf of Mexico, we assessed the potential for using a hypervariable region sequence of the mitochondrial DNA control region (mtCR) as a genetic tag. Total genomic DNA was extracted from 27 red snapper, amplified, cloned, sequenced, and aligned to determine the genetic structure of the mtCR. A hypervariable region of 170 base pairs (bp) was identified where 23 of the 27 red snapper were found to be unique. To assess the potential of this region for use as a genetic tag, total genomic DNA was extracted from additional red snapper and amplified utilizing primers that flank the hyperva riable region. These samples are currently being sequenced. Preliminary analysis of the sequence data indicates that the level of polymorphism in the hypervariable region may be useful in specific genetic population assessments at release sites.


Virginia Shervette [1][*], Harriet Perry [2], Patricia M. Biesiot [1], Kirsten Larsen [2], and James R. Warren [2], (1.) University of Southern Mississippi, Hattiesburg, MS 39406-5018 and (2.) Gulf Coast Research Laboratory, Institute of Marine Sciences, University of Southern Mississippi, Ocean Springs, Mississippi Ocean Springs is a city in Jackson County, Mississippi (USA), about 2 miles east of Biloxi. It is part of the Pascagoula, Mississippi Metropolitan Statistical Area. The population was 17,225 at the 2000 census.

The town has a reputation as an "arts community.

Many marine organisms are restricted to habitats which provide essential refuge. The stone crab, Menippe adina, is associated with rock rubble jetties and oyster reefs in northern Gulf of Mexico estuaries. Lack of extensive hard bottom habitat, competition for limited space, and predation all operate to influence population levels. Establishment of low profile artificial reefs in Mississippi Sound provided an opportunity to investigate refuge limitation in juvenile stone crabs and to examine the roles of predation and inter-specific competition in controlling population levels. There is strong evidence that size-specific refuge limitation exerts control on both population size structure and density of stone crabs. Stone crab larvae and small juveniles (10-24 mm carapace carapace (kâr`əpās), shield, or shell covering, found over all or part of the anterior dorsal portion of an animal. In lobsters, shrimps, crayfish, and crabs, the carapace is the part of the exoskeleton that covers the head and thorax  width) are relatively abundant in Mississippi Sound and the species does not appear to be recruitment limited. Larger juveniles are less common and their numbers may be related to quantity and quality of suitable habitat. Competition for avai lable habitat may be acute between M. adina and other xanthid crabs (Eurypanopeus depressus and Panopeus simpsoni). Competition for habitat also occurs between stone crabs and the toadfish toadfish, common name for the sluggish, bottom-feeding fishes of the genus Opsanus, found in the shallow waters from New Jersey to the Caribbean. Toadfishes feed almost entirely on crustaceans and small fishes. , Opsanus beta. Toadfish collected in the study area are active predators on the three xanthid taxa taxa: see taxon. .


Ship Isle

1:20 MITOCHONDRIAL mitochondrial

pertaining to mitochondria.

mitochondrial RNAs
a unique set of tRNAs, mRNAs, rRNAs, transcribed from mitochondrial DNA by a mitochondrial-specific RNA polymerase, that account for about 4% of the total cell RNA that

Glenn M. Hendrix [*], Kenneth C. Stuck, and Walter D. Grater, Gulf Coast Research Laboratory, University of Southern Mississippi, Ocean Springs, MS 39564

Three species of Paralichthys occur in the Northern Gulf of Mexico: P. albigutta, P. lethostigma, and P. squamilentous. Paralichthys lethostigma is an important recreational and commercial species in Mississippi and is listed as a secondary species for possible enhancement by the U.S. Gulf of Mexico Stock Enhancement Consortium. There is little published information available on the genetics of Paralichthys, and no published mitochondrial DNA sequences for the three species in this study. We conducted this study to determine the potential of using 12S rRNA (commonly used for taxonomic identification of marine finfish) as a genetic marker for the identification of Paralichthys species from the northern Gulf. Total DNA DNA: see nucleic acid.
 or deoxyribonucleic acid

One of two types of nucleic acid (the other is RNA); a complex organic compound found in all living cells and many viruses. It is the chemical substance of genes.
 was extracted from five individuals of each species and used as template for a PCR PCR polymerase chain reaction.

polymerase chain reaction

Polymerase chain reaction (PCR) 
 that amplified approximately 900 base pairs of the 12S gene. The resulting PCR fragments were gel purified, T/A T/A Turnaround
T/A Traffic Analysis
T/A Time/Attendance
T/A Trading As
T/A Trans America
T/A Tonsils/Adenoids
T/A Training/Allowance
T/A Traction/Advantage (BF Goodrich)
T/A Team Assistance
T/A Table of Allowance
 cloned, and sequenced. The sequences were then screened for 30 restriction endonuclease sites, and it was found that a combination of Msp I and Xho I could be used to separate the three species. This molecular technique may be particularly useful for identifying otherwise indistinguishable specimens, such as newly hatched larvae.


Michele Routhier [l][*], Robert A. Amone [2], and Richard Gould [2], (1.) University of Southern Mississippi, Stennis Space Center, MS 39529 and (2.) Naval Research Laboratory, Stennis Space Center, MS 39529

Surface optical and physical properties of the Chesapeake Bay plume are examined through a tidal cycle during the Chesapeake Bay Outflow Plume Experiment (COPE II, May 1997) to determine small scale structure of mixing processes. We describe the movement of the plume using surface salinity and the optical properties of absorption (a) and scattering (b) coefficients. Coupled with the shipboard measurements, five aircraft hyperspectral images over a 6-hour partial tidal cycle show the changing surface patterns. Surface a and b values from an ac9 instrument decreased along a 24 km offshore ship transect tran·sect  
tr.v. tran·sect·ed, tran·sect·ing, tran·sects
To divide by cutting transversely.

[trans- + -sect.
. The absorption coefficient at 412 nm decreased from 1.3 m-1 inside the plume to 0.1 m-1 outside, and the scattering coefficient at 555 nm decreased from 3.30 m-1 to 1.05 m-1. We compare the ac9 a and b measurements with estimates derived from the hyperspectral imagery. The short time and space scales resolvable with hyperspectral aircraft imagery makes it a unique tool for characterizing plume dynamics.


Harriet Perry [l][*], Wayne Isphording [2], Christine Trigg [1], Newton Fawcett [3], Richard Waller [1], and Kirsten Larsen [1], (1.) University of Southern Mississippi, Institute of Marine Science, Gulf Coast Research Laboratory, Ocean Springs, MS 39566; (2.) University of South Alabama The University of South Alabama is a public, doctoral-level university in Mobile, Alabama, USA. It was created by the Alabama Legislature in 1963, and replaced existing extension programs operated in Mobile by the University of Alabama. , Mobile, AL 36688; and (3.) University of Southern Mississippi, Hattiesburg, MS 39406-5043

The geryonid, Chaceon quinquedens, is common in slope waters of the eastern Gulf of Mexico (GOM GOM - Good Old MAD.

Don Boettner, U Mich. MAD for the IBM 360. Parts of the MTS time-sharing system were written in GOM.
) at depths greater than 400 fathoms. Highest concentrations of red crabs occur in the north central GOM in an area influenced by run-off from the Mississippi River. Their geographic location, close association with sediments, and protracted pro·tract  
tr.v. pro·tract·ed, pro·tract·ing, pro·tracts
1. To draw out or lengthen in time; prolong: disputants who needlessly protracted the negotiations.

 intermolt period favor accumulation of metal contaminants. Ten male and ten female red crabs were collected using molded plastic traps. Sediment and water samples were taken concurrently with trap collections. Crabs were dissected at sea and samples of gill, muscle, and hepatopancreas The hepatopancreas is an organ of the digestive tract of arthropods, gastropods and fish. It provides the functions which in mammals are provided separately by the liver and pancreas.  taken. Eggs were removed from ovigerous females. Trace elements/heavy metals were measured using inductively coupled plasma An inductively coupled plasma (ICP) is a type of plasma source in which the energy is supplied by electrical currents which are produced by electromagnetic induction, that is, by time-varying magnetic fields.  spectrophotometry spectrophotometry

Branch of spectroscopy dealing with measurement of radiant energy transmitted or reflected by a body as a function of wavelength. The measurement is usually compared to that transmitted or reflected by a system that serves as a standard.
 and atomic absorption spectrophotometry. Concentrations for sediment and water were approximately [less than or equal to] levels previously reported for the GOM or other oceanic areas. Cu, Zn, As, Cd, Cr, Sn, Sc, and Hg levels in most wet tissues were found to be significantly higher than sediment levels. Bioaccumulation bi·o·ac·cu·mu·la·tion
The increase in the concentration of a substance, especially a contaminant, in an organism or in the food chain over time.
 was most evident in the hepatopancreas, followed by the gills, and muscle tissue. Mean concentrations of Cr and Pb in wet muscle tissue were greater than FDA FDA
Food and Drug Administration

FDA, See Food and Drug Administration.

FDA, the abbreviation for the Food and Drug Administration.
 levels of concern.


Richelle A. Hanson [*] and Charlotte A. Brunner, University of Southern Mississippi, Stennis Space Center, MS 39529

The goal of this study is to determine paleoenvironmental changes in the Pearl River Marsh using foraminiferal assemblages. The present-day marsh, located on the border of Mississippi and Louisiana, is microtidal with salinity ranging from fresh to brackish ([less than]12). Four 3" diameter vibra-cores were taken in a transect from the freshwater marsh, through the brackish marsh, and ending near Lake Bourne Bourne, town (1990 pop. 16,064), Barnstable co., SE Mass., crossed by Cape Cod Canal; settled 1627, inc. 1884. Bourne Bridge (1935), across the canal, made the town an entry point to Cape Cod and a resort and commercial center. . The cores, which averaged 6 m in length, penetrated Holocene peats and sands, with three piercing the underlying Pleistocene Prairie Formation. A census of foraminifers was taken at 50-100 cm intervals in each core by microscope observation of wet samples. Consistent with the present-day salinity gradient, the assemblage from the upper 100 cm of the mid-marsh core is dominated by brackish, benthic ben·thos  
1. The collection of organisms living on or in sea or lake bottoms.

2. The bottom of a sea or lake.

, agglutinated foraminifers with a trace of saline species. In contrast, the assemblage includes only brackish species from 152-250 cm. There is an interval of barren peat from 305-369 cm. The assemblage below is similar to the surface assemblage. These results are interpreted as the response of the foraminiferal assemblage to changes in salinity caused by formation of the St. Bernard Lobe of the Mississippi River Delta For other uses, see Mississippi Delta (disambiguation)

The Mississippi River Delta is the modern area of land (the river delta) built up by alluvium deposited by the Mississippi River as it slows down and enters the Gulf of Mexico.
. Prior to [sim]3500 yBP, the Pearl River Marsh experienced open exchange of fresh and salt water. During formation and activity between [sim]3500 and 1500 yBP, the St. Bernard Lobe isolated the Pearl River from the salt input from the Mississippi Sound. During subsidence of the St. Bernard Lobe, infiltration of sound waters progressively increased, leading to the present-day, brackish salinity.

2:40 Break; Divisional Business Meeting

The use of plants and trees to remove or neutralize contaminants, as in polluted soil or water.


See under bioremediation.

Julia S. Lytle [*], Thomas F. Lytle, and Larry Stewart, University of Southern Mississippi, Institute of Marine Sciences, Gulf Coast Research Laboratory, Ocean Springs, MS 39564

Contaminated sediments are a major concern in the marine environment. A promising alternate procedure for cleaning up contaminated sediments is through the use of plants, referred to as phytoremediation. Plants naturally ooze exudates from their roots that act as surfactants to release sediment-bound contaminants into the sediment pore water. Once contaminants are dissolved into the water, they are bioavailable to plants and other organisms. Uptake and degradation by organisms can effectively remediate the sediments. An experiment was designed to test the effect of two levels of a nonionic surfactant Surfactant Definition

Surfactant is a complex naturally occurring substance made of six lipids (fats) and four proteins that is produced in the lungs. It can also be manufactured synthetically.
 on the bioavailability bioavailability /bio·avail·a·bil·i·ty/ (bi?o-ah-val?ah-bil´i-te) the degree to which a drug or other substance becomes available to the target tissue after administration.

 of a polynuclear polynuclear /poly·nu·cle·ar/ (-noo?kle-er) having several nuclei; said of cells.

pol·y·nu·cle·ar or pol·y·nu·cle·ate or pol·y·nu·cle·at·ed
 aromatic hydrocarbon (PAH), fluoranthene, for uptake by the estuarine plant, Sesbania vesicaria. The objective of the study was to determine what surfactant level is needed to effectively make sand-bound fluoranthene bioavailability to S. vesicaria. Seven microcosms, each containing three replicate treatments, were prepared with and without PAH contaminated sands and satur ated with either distilled water, water with a low surfactant concentration, or water with a high surfactant concentration. Controls were prepared for each treatment. After a six-week exposure, fluoranthene concentrations in plant tissue and sands were measured using a fluorescence spectrophotometer spectrophotometer, instrument for measuring and comparing the intensities of common spectral lines in the spectra of two different sources of light. See photometry; spectroscope; spectrum. . Uptake was expected to be greatest in plants growing in contaminated sands wetted with the higher surfactant concentrations. Plant growth ( height, biomass and root elongation) was correlated with plant uptake of fluoranthene.


Donna Thomas [1][*], Robert A. Arnone [2], Richard P. Stumpf [3], Karen Steidinger [4], and Brad Pederson [5], (1.) University of Southern Mississippi, Stennis Space Center, MS 39529; (2.) Naval Research Laboratory, Stennis Space Center, MS 39529; (3.) NOAA National Ocean Service, Silver Spring, MD 20910; (4.) Florida Department of Environmental Protection The Florida Department of Environmental Protection (FDEP) is the agency in Florida's government charged with most functions relating to environmental quality in the state. [1] History
By the mid-1960s, when the U.S.
, St. Petersburg, FL 33701; and (5.) Mote Marine Laboratory Mote Marine Laboratory (and Aquarium) is a not-for-profit research and educational institution with an aquarium open to the public 365 days a year. Founded by Dr. Eugenie Clark in 1955 in Cape Haze, Florida, the early years of the laboratory specialized in shark research. , Sarasota, FL 34326

We traced the spatial and temporal extent of a Gymnodinium breve BREVE, practice. A writ in which the cause of action is briefly stated, hence its name. Fleta, lib. 2, c. 13, Sec. 25; Co. Lit. 73 b.
     2. Writs are distributed into several classes.
 bloom off of the Florida panhandle from August through October 1999 using a time series of inherent optical properties (spectral absorption and back-scattering) and chlorophyll concentration derived from SeaWiFS imagery. High cell counts of Gymnodinium breve were correlated with SeaWiFS-derived chlorophyll concentration. We discuss the influence of elevated cell counts on the backscattering to absorption ratio and chlorophyll concentration derived from SeaWiFS. Improved atmospheric corrections and new iterative techniques allow SeaWiFS algorithms to be extended into the coastal environment to derive the bio-optical properties. SeaWiFS provides real-time trends of the bio-optical properties that help identify the environmental and biological cues used to predict the occurrence of future outbreaks of harmful algal blooms.


Harriet Perry [*], Kirsten Larsen, Sara LeCroy, Christine Trigg, and James R. Warren, Institute of Marine Science, University of Southern Mississippi, Gulf Coast Research Laboratory, Ocean Springs, MS 39566-7000

Artificial reefs serve as fish attractants and may increase production of some species by increasing habitat. Although over twenty low profile artificial reefs (oyster shell, concrete rubble, limestone gravel) have been constructed in Mississippi inshore waters, there are no data on reef community structure or the association of fish populations with these reefs. A study of the faunal assemblages associated with reef colonization in Mississippi Sound was begun in December 1998 as part of a larger program to assess productivity of these reefs in relation to recreational fishing opportunities. Colonization was studied by placing a series of crates filled with 0.025 [m.sup.3] of limestone gravel or oyster shell on a newly created limestone/shell reef. A portion of the crates are sampled at 3, 6, 9 or 12-month intervals and all organisms removed and counted. The present data are taken from the initial three-month colonization period. Amphipods were the most abundant macro-crustaceans associated with the reef mat erials. Two genera of free-living gammarideans dominated the amphipod fauna on both substrates: Melita and Apocorophium. There was no significant difference in the species composition between the two substrate types. Melita nitida was extremely abundant; Melita longisetosa and Apocorophium louisianum were also common in samples. Monocorophium acherusicum was found only in oyster shell samples in limited numbers.

4:00 Divisional Poster Session


Israel Anderson Denham [1][*], James R. Warren [2], and Jude LeDoux [2], (1.) Mississippi Gulf Coast Community College Mississippi Gulf Coast Community College consists of four campuses and four centers: the main campus, located in Perkinston, Mississippi[1]; the Jackson County Campus, in Gautier[2]; the Jefferson Davis Campus, in Gulfport[3]; the Community Campus, a , Gautier, MS 39533 and (2) Gulf Coast Research Laboratory, University of Southern Mississippi, Ocean Springs, MS 39566-7000

Artificial reefs have become important to the Mississippi Gulf Coast The Mississippi Gulf Coast refers to the three Mississippi counties which lie on the Gulf of Mexico: Hancock County, Mississippi, Harrison County, Mississippi, and Jackson County, Mississippi.  recreational fisheries. These inshore reefs promote tourism along the Gulf Coast by attracting sport fishermen. Over twenty inshore artificial reefs have been established in the Mississippi Sound. One reef (Gulf Park Estates) was chosen to provide information on the benthic organisms that colonize this reef and utilization of the reef by local fish species. During this study, samples were taken from the reef at three month intervals using entanglement gear. All fish were returned to the Gulf Coast Research Laboratory where stomachs were removed and preserved. Stomachs were removed from Menticirrhus americanus, Cynoscion arenarius, C. nebulosus, Leiostomus xanthurus, and Paralichthys lethostigma, and the stomach contents were analyzed. Ostracods, copepods, and cumaceans were found within stomachs from L. xanthurus. Stomach contents from M. americanus consisted primarily of unidentifiable Adj. 1. unidentifiable - impossible to identify
identifiable - capable of being identified
 fish remains. Cynoscion nebulosus and C. arenarius stom ach contents consisted predominately of fish and shrimp.


Jennifer Hale, J.L. Scott Marine Education Center and Aquarium, University of Southern Mississippi, Biloxi, MS 39530

Sea Turtles have gained worldwide attention due to their endangered status. Human activity has contributed to their decline. Populations have been decimated by hunting, either for consumption or for the use of the shell in ornamentation, and by drowning as a result of being taken as by-catch. To add to their troubled future, many sea turtles are becoming infected with the fibropapilloma tumors which are growths that are virally caused. Discover how and why these fatal tumors are rapidly spreading through the sea turtle populations and what measures are being taken to help these animals survive. Materials will be provided to the attendees.


Christopher Hayesl [1][*], Kirsten Larsen [2], Harriet Perry [2], Christine Trigg [2], and James R. Warren [2], (1.) Cooperative Intern Program, Mississippi Gulf Coast Community College, Gautier, MS 39553 and (2.) Gulf Coast Research Laboratory, University of Southern Mississippi, Ocean Springs, MS 39566-7000

The establishment of low profile artificial reefs composed of limestone gravel and/or oyster shell in coastal waters of Mississippi provided the opportunity to study the seasonal occurrence, abundance, and recruitment of three species of xanthid crabs (Eurypanopeus depressus, Panopeus simpsoni, and Menippe adina) associated with these structures. Menippe adina supports limited commercial fisheries in the northern Gulf of Mexico. The mud crabs, E. depress us and P. simpsoni, are ecologically important components of oyster reef communities. Plastic crates filled with either limestone or oyster shell substrate were placed on an existing low profile reef located in approximately 1.5 m of water. Four crates of each substrate were placed on the reef in each season (winter, spring, and summer). Crates were removed from the water after three months. Crabs were picked from samples and identified to species. Each crab was measured and weighed and a total number and weight by species recorded. Egg-bearing females were n oted. Recruitment occurred for all species in the late summer/early fall.


Nicole A. Housley [1][*], Thomas F. Lytle [2], Kenneth McMurtrey [3], and Julia S. Lytle 2, (1.) Cooperative Intern Program, Mississippi Gulf Coast Community College, Gautier, MS 39533; (2.) Gulf Coast Research Laboratory, University of Southern Mississippi, Ocean Springs, MS 39566-7000; and (3.) University of Southern Mississippi, Hattiesburg, MS39406

Of available methods to remediate metal contaminated soil, phytoremediation offers significant advantages. Use of plants to remove/immobilize metals in soils is effective in many applications and non-destructive to natural environmental systems. We have examined ability of coastal marsh plants to remove metals from contaminated sediments and the mechanisms of removal and storage in the plants. Many terrestrial and aquatic plants produce metal-binding phytochelatins, synthesized from glutathione in response to heavy metals. To determine whether marsh plants also produce these compounds and may serve to sequester sequester v. to keep separate or apart. In so-called "high-profile" criminal prosecutions (involving major crimes, events, or persons given wide publicity) the jury is sometimes "sequestered" in a hotel without access to news media, the general public or their  and store these metals, we have tested Juncus roemerianus and Spartina alterniflora by spiking associated sediments with Cd and placing barriers around plants to restrict movement of the Cd. After a two month exposure, exposed and control plants were removed, tissue extracted, and phytochelatins derivatized before separation and quantification by HPLC. Associated sediments were analyzed for Cd and othe r metals (Cu, Zn, Ag, Cr, Ni, Pb, Co). These metals were fractionated into bioavailable and non-bioavailable by a simultaneous extraction procedure so that the presence and level of tissue phytochelatins could be related to quantity of heavy metals readily available to plants.


Elizabeth A. Quave [1][*], Cynthia A. Moncreiff [2], Todd A. Randall [2], and John D. Caldwell [2], (1.) Cooperative Intern Program, Mississippi Gulf Coast Community College, Gautier, MS 39553 and (2.) Gulf Coast Research Laboratory, University of Southern Mississippi, Ocean Springs MS 39566-7000

Toxic and harmful algal bloom species that can occur in northern Gulf of Mexico waters will be featured on a web site under development by the authors. This site will include the classic "red tide" alga Gymnodinium breve, several species of Prorocentrum, Dinophysis caudata, and Alexandrium monilatum, plus several of the roughly 20 other potentially harmful algal algal

pertaining to or caused by algae.

algal infection
is very rare but systemic and udder infections are recorded. See protothecosis.

algal mastitis
the algae Prototheca trispora and P.
 species that are known to occur in coastal Mississippi waters. The site will be targeted toward the general public as a source of basic details on algal blooms, their causes, and the potential effects they can have on people, wildlife, and the environment. Species accounts will consist of photomicrographs, line drawings, and general life history information plus the possible effects of exposure to these harmful algal blooms. Videotapes of harmful algal blooms that occur during the project time frame will also be made using a microscope imaging system to aid in identification and will be included on the site.


Alison Sharpe, J.L. Scott Marine Education Center and Aquarium, Institute of Marine Sciences, University of Southern Mississippi, Biloxi, MS 39530

Along the coastal areas of the United States, the brown pelican can be seen soaring effortlessly over the marine and estuarine waters. Just a few years ago, the brown pelican was listed as an endangered species throughout its range. Today, in most areas, they have been removed from their endangered status. In September of 1998 Hurricane Georges struck the Mississippi coast, leaving a wake of destruction along tour mainland and decimating the brown pelican rookeries along the Chandeleur Island chain. This presentation will discuss the nesting problems and whether natural disasters, like hurricanes, might be a contributing factor that could return these marine birds to the endangered species list.


Larry Stewart [*], Julia S. Lytle, and Thomas F. Lytle, University of Southern Mississippi, Institute of Marine Sciences, Gulf Coast Research Laboratory, Ocean Springs, MS 39564

Bioremediation is one of the most promising developments in modern pollution control. Plants contain enzymes that can either detoxify de·tox·i·fy
1. To counteract or destroy the toxic properties of a substance.

2. To remove the effects of poison from something, such as the blood.

 the contaminant, or can stimulate bacteria in the rhizosphere rhi·zo·sphere  
The soil zone that surrounds and is influenced by the roots of plants.


The soil zone that surrounds and is influenced by the roots of plants.
 which can degrade the contaminant. Plants release materials through their roots, referred to as exudates. This nutrient rich material is a food source to bacteria and also acts as a surfactant to solubilize sol·u·bi·lize
To make substances such as fats soluble in water by the action of a detergent or similar agent.
 soil-bound contaminants onto the plant roots. Some plant species are able to take up the contaminant into their tissue. However, plants cannot survive without defense mechanisms that allow them to cope with these contaminants. Many estuarine plants have evolved various mechanisms of defense. Glutathione, a known plant antioxidant can relieve oxidative stress and as an enzyme, can degrade toxins. Ascorbic acid is another antioxidant plants synthesize in response to oxidative stress. In a laboratory designed study, Sesbania vesicaria was grown in sands coated with 10 ppm fluoranthene. Two levels of surfactants were added to test containers to simulate the exudates' ability to reduce surface tension and solubilize contaminants. After 6 weeks of exposure, plant tissue (root and leaf) was extracted and glutathione and ascorbic acid levels were measured. The objective of the study was to determine what concentration level of surfactant is needed to solubilize sediment-bound fluoranthene and to assess the effect of elevated surfactant levels as a function of plant response.


Ship Isle


Ashley D. Trahan [1][*], Larry Stewart [2], Julia S. Lytle [2], and Thomas F. Lytle [2], (1.) University of Southern Mississippi, Hattiesburg, MS 39406 and (2.) University of Southern Mississippi, Gulf Coast Research Laboratory, Ocean Springs, MS 39564

Contaminated sediments pose a significant threat to environmental and human health. They are a major sink for a wide range of pollutants. An alternative method for cleaning up these waste sites is the use of organisms, primarily bacteria, and plants. Plants release exudates that act as a nutrient source for bacteria, and these materials also act as a surfactant which can help to solubilize sediment-bound contaminants. Bacteria have been identified that produce exudates specifically to facilitate the degradation of highly insoluble organic compounds and presumably plants may also behave similarly. Exudates can vary in composition depending on species and maturity of plant and the root rhizosphere. To better understand the role of exudates as surfactants, an experiment was designed to characterize exudates from Spartina alterniflora collected at different time intervals of their growth. Plants were grown under hydroponic conditions in order to easily collect plant water exudates. Ionic and nonionic standards w ere used to develop a technique for characterizing surfactants; using an Orion surfactant electrode. No evidence of cationic cationic

having qualities dependent on having free cations available.

cationic detergents
are wetting agents that disrupt or damage cell membranes, denature proteins and inactivate enzymes.
 or anionic an·i·on  
A negatively charged ion, especially the ion that migrates to an anode in electrolysis.

[From Greek, neuter present participle of anienai, to go up : ana-, ana-
 surfactants was found and nonionic surfactants analysis will require isolation from exudates before further analysis. Other experiments established the enhanced solubility of certain polynuclear aromatic hydrocarbons in aqueous solutions containing the S. alterniflora exudates and suggested the role these exudates may play in mobilization of sediment contaminants. Exudates were frozen for later analysis when further development of nonionic surfactant techniques are complete.


Kimberly A. Kelly [1][*], Steven E. Lohrenz [1], and Gary L. Fahnenstiel [2], (1)University of Southern Mississippi, Institute of Marine Sciences, Stennis Space Center, MS 39529 and (2)NOAA Great Lakes Environmental Research Laboratory, Muskegon, MI 49441

Microphotometric techniques were used to measure the light absorption characteristics of phytoplankton at the single-cell level. Typically, phytoplankton absorption has been determined at the community level using bulk measurements. Use of microphotometric techniques enables the direct measurement of the in vivo absorption efficiency factor [Q.sub.a](l) of individual cells. Light is an important variable controlling the development of the spring diatom diatom (dī`ətŏm', -tōm'), unicellular organism of the kingdom Protista, characterized by a silica shell of often intricate and beautiful sculpturing. Most diatoms exist singly, although some join to form colonies.  community in Lake Michigan. This study examined the impact of a recurrent coastal plume on variations in light availability and the associated absorption characteristics of three phytoplankton groups in the vicinity of the plume. Three groups were examined including Aulacoseira islandica, Aulacoseira subarctica and smaller centric (8-10 mm) diatoms. The magnitude of [Q.sub.a](l) for all phytoplankton groups did not vary significantly with depth or time or across plume gradients. However, a comparison between species revealed that the spectral shape of [Q.sub.a ](l) differed in the blue wavelengths. The centric diatoms had a higher blue-to-red ratio than the Aulacoseira species. The possibility is considered that such differences in light absorption efficiency impart a competitive advantage that could contribute to the higher abundance of centric diatoms in plume assemblages.

9:20 HISTOPATHOLOGY his·to·pa·thol·o·gy
The science concerned with the cytologic and histologic structure of abnormal or diseased tissue.

The study of diseased tissues at a minute (microscopic) level.

Rena Krol [1][*], K. Vijayan[2], Jeffrey M. Lotz[1], and Robin Overstreet[1], (1)Institute of Marine Sciences, Ocean Springs, MS 39566 and (2)Central Institute of Brackishwater Aquaculture aquaculture, the raising and harvesting of fresh- and saltwater plants and animals. The most economically important form of aquaculture is fish farming, an industry that accounts for an ever increasing share of world fisheries production. , Madras, India

White spot virus (WSV WSV Wassersportverein (German: Water Sports Club)
WSV Winterschlussverkauf (German: winters-end sales)
WSV Wheelchair Sports Victoria (Australia)
WSV Wärmeschutzverordnung
), a major disease-causing agent in the shrimp aquaculture industry in the eastern hemisphere, can also infect Procambarus clarkii, the economically important freshwater crayfish crayfish or crawfish, freshwater crustacean smaller than but structurally very similar to its marine relative the lobster, and found in ponds and streams in most parts of the world except Africa. Crayfish grow some 3 to 4 in. (7.6–10.  from the southeastern U.S. This study examines WSV in Procambarus clarkii by transmission electron microscopy “TEM” redirects here. For other uses, see TEM (disambiguation).

Transmission electron microscopy (TEM) is an imaging technique whereby a beam of electrons is transmitted through a specimen, then an image is formed, magnified and directed to appear either
 and light microscopy and compares the viral infection with that found in shrimp. Captive crayfish were injected with an inoculum inoculum /in·oc·u·lum/ (-ok´u-lum) pl. inoc´ula   material used in inoculation.

n. pl.
 of WSV-infected tissue from Penaeus monodon from India. In another experiment, crayfish were fed tissues of Litopenaeus vannamei which had been exposed to WSV-infected P. monodon. Gill, foregut foregut /fore·gut/ (-gut) the endodermal canal of the embryo cephalic to the junction of the yolk stalk, giving rise to the pharynx, lung, esophagus, stomach, liver, and most of the small intestine. , and cuticular cu·ti·cle  
1. The outermost layer of the skin of vertebrates; epidermis.

2. The strip of hardened skin at the base and sides of a fingernail or toenail.

3. Dead or cornified epidermis.

 epidermis were prepared for light microscopy. Foregut was prepared for transmission electron microscopy (TEM TEM

1. transmission electron microscope.

2. triethylenemelamine.

3. transmissible encephalopathy of mink.
). By the second day post injection, animals exhibited lethargy and morbidity. No white spots appeared in the exoskeleton exoskeleton /exo·skel·e·ton/ (-skel´e-ton) a hard structure formed on the outside of the body, as a crustacean's shell; in vertebrates, applied to structures produced by the epidermis, as hair, nails, hoofs, teeth, etc. . By the fourth day, cumulative mortality was 100%. The feeding experiments ran for 17 days. Cumulative mortality at that time was 88% for crayfis h fed WSV-infected P. monodon and 50% for crayfish fed L. vannamei exposed to WSV-infected P. monodon. Histology revealed WSV infection in cell nuclei of the three tissues examined. TEM of foregut showed bacilliform ba·cil·li·form
Having a rodlike shape.


having the appearance of a bacillus.
 virions 240-310 rim long by 80-100 nm wide. Some viions were found in membrane-bound vesicles in the cell cytoplasm. This study was funded in part by USDA USDA, See United States Department of Agriculture.
, CSREES CSREES Cooperative State Research, Education, and Extension Service (USDA)  Award No. 98-38808-6019.


Main article: Mississippi River
The Lower Mississippi River is the portion of the Mississippi River downstream of Cairo, Illinois. From the confluence of the Ohio River and Upper Mississippi River at Cairo, the Lower flows just under 1600

Alan M. Shiller [1][*], Julie Havens[1], and Robyn Hannigan [2], (1.)University of Southern Mississippi, Stennis Space Center, MS 39529 and (2.)Old Dominion University “ODU” redirects here. For other uses, see ODU (disambiguation).

The university was recently named one of the best colleges in the Southeast by The Princeton Review.
, Norfolk, VA 23529

Rare earth elements (REEs) form an unique series in which incremental changes in chemical properties occur across the series as a result of decreasing ionic radius as f-shell electrons are filled in. In general it is found that heavier REEs are preferentially complexed in solution whereas lighter REEs are preferentially sorbed sorb 1  
tr.v. sorbed, sorb·ing, sorbs
To take up and hold, as by absorption or adsorption.

[Back-formation from absorb and adsorb.
 to particle surfaces. Most of the REEs are found in the trivalent trivalent /tri·va·lent/ (tri-va´lent) having a valence of three.

Having valence 3.

 oxidation state in natural waters. However, oxidation of Ce(III) to Ce(IV) and subsequent scavenging scavenging

of anesthetic. See anesthetic scavenging.
 removal of Ce(IV) can lead to lower than expected dissolved concentrations of this element (the so-called Ce anomaly). In our study, dissolved REE concentrations were determined in a monthly time series of the lower Mississippi River which was conducted from October 1991 to December 1993. Overall our results agree with limited previous investigations; i.e., the river shows enrichment of heavy REEs relative to light REEs and also has a significant Ce anomaly. However, the previous investigations relied on only single sampl es from the river. Our seasonal investigation reveals significant temporal variations in the river's REE chemistry. In particular, we observe substantial (and generally correlated) variations in heavy REE enrichment and Ce anomaly. Overall the most fractionated (with respect to crustal composition) waters generally occur in winter and spring and the least fractionated waters occur in summer.


cause of severe losses in juvenile prawns Penaeus vanammei.

Anne Marie Moore [*], Jeffrey M. Lotz, and Verlee Breland, Gulf Coast Research Laboratory, Institute of Marine Sciences, University of Southern Mississippi, Ocean Springs, MS 39566-7000

Taura syndrome virus (TSV TSV - tab-separated values ) and white spot virus (WSV) are the two most important shrimp pathogens affecting aquaculture of the white-legged shrimp Litopenaeus vannamei in the Western Hemisphere. A matter of days after exposure to TSV the mortality in a population of cultured L. vannamei is typically 75% whereas mortality after exposure to WSV is likely to be 90%. As part of an ongoing study on the pathology and epidemiology of TSV and WSV we undertook to compare the survival of naive L. vannamei and survival of previously exposed L. vannamei to re-challenge with the homologous virus. Shrimp between 1 and 5 g were exposed by feeding infected shrimp cephalothoraces at a rate of 3% body weight. Survivors of such primary exposures were used for subsequent re-exposures to virus. Several re-exposure experiments were performed with the TSV and WSV survivors. In a typical experiment 12 TSV survivors were challenged with TSV and 10 WSV survivors were challenged with WSV. After 21 days the TSV survivors challenged wit h TSV had 0% mortality whereas the WSV survivors rechallenged with WSV had 100% mortality after 5 days. It appears that surviving a TSV challenge imparts resistance to rechallenge with TSV whereas survival of a WSV challenge does not impart resistance to re-challenge with WSV. Partial funding was provided through USDA/CSREES grant number 98-388086019.

10:20 Break

10:40 CALIBRATION OF SPLIT-FLOW THIN-CELL (SPLITT) FRACTIONATION fractionation /frac·tion·a·tion/ (frak?shun-a´shun)
1. in radiology, division of the total dose of radiation into small doses administered at intervals.


Toshi Uozumi [*] and Alan M. Shiller, University of Southern Mississippi, Stennis Space Center, MS 39529

Split-flow thin-cell (SPLITT) fractionation is a continuous particle separation technique which is relatively new and still under development. SPLITT is a rapid hydrodynamic hy·dro·dy·nam·ic   also hy·dro·dy·nam·i·cal
1. Of or relating to hydrodynamics.

2. Of, relating to, or operated by the force of liquid in motion.
 separation technique applicable to environmental samples. Separation is achieved using the gravitational grav·i·ta·tion  
1. Physics
a. The natural phenomenon of attraction between physical objects with mass or energy.

b. The act or process of moving under the influence of this attraction.

 settling of particles as they traverse the SPLITT cell. Each operation separates particles into two fractions at a certain cutoff diameter which can be easily changed by adjusting flow rates (i.e., the transit time of particles through the SPLITT cell). In theory, the necessary flow rates are predictable by first principle calculations; however, the actual cutoff diameter does not always match the theoretical cutoff diameter. This seems to be the case especially when a sample contains particles with differing densities. Therefore, the cutoff diameter requires calibration by measuring the actual size of the separated particles. This procedure can be done by using a combination of SEM and an image-processing program with a capability of quantifying particle dimensions. In our study, river particles ( 1-64 microns) taken from the Mississippi River are separated into four different size fractions using the SPLITT technique. The separation of Mississippi River suspended particles is a first step in work aimed at understanding the nature of seasonal changes in the surface area of the river particles.


Steven E. Lohrenz [1][*], Christopher L. Carroll [1], and Alan D. Weidemann [2], (1.) University of Southern Mississippi, Stennis Space Center, MS 39529 and (2.) Naval Research Laboratory, Stennis Space Center, MS 39529

Critical to our understanding of coastal ecosystems is knowledge of phytoplankton distributions. Our ability to characterize phytoplankton distributions in coastal environments has the potential to be greatly enhanced by the application of remote sensing. One factor contributing to uncertainty in estimates of pigment concentrations by remote sensing is variability in pigment absorption coefficients. The coastal region south of Chesapeake Bay is an excellent area to examine consequences of variability in phytoplankton communities. Various studies have noted differences in phytoplankton size and taxonomic composition among different water masses in the region. Our objective was to examine whether variations in pigment spectral absorption accompanied the differences in phytoplankton size and taxonomic composition. The shapes of the pigment absorption spectra for near-shore low salinity (plume) stations were characterized by lower blue-to-red absorption ratios than was the case for higher salinity stations furth er offshore. The spectral shapes of the [less than]3 micron size fractions at the near-shore stations were similar to those observed at the offshore stations indicating that the observed differences in spectral shape were size-related. Our findings demonstrate that characterization of pigment absorption by remote sensing must take into account variations in phytoplankton size and associated absorption properties.


Jeffrey Lyons [1][*], Nathaniel Smith [2], Thomas F. Lytle [3], and Julia S. Lytle [3], (1.) Vanderbilt University, Nashville, TN; (2.) Alcom State University, Alcorn State, MS 39096; and (3.) University of Southern Mississippi, Gulf Coast Research Laboratory, Ocean Springs, MS 39564

Phytoremediation, vegetation-enhanced bioremediation, offers an attractive alternative to conventional means of decontaminating sediments. Little research has explored use of coastal marsh plants in phytoremediation of sediments. Three prominent marsh plants, Juncus roemerianus, Spartina alterniflora, and Sagittaria lancifolia and associated sediments were collected from a Department of Defense site with known elevated levels of heavy metals, from Ocean Springs Harbor and also from a control site in Ocean Springs. Plant roots and leaves were acid digested and analyzed by either flame or furnace atomic absorption spectrophotometry for Cu, Zn, Co, Ag, Cr, Cd, Ni and Pb. Sediments were digested to remove "bioavailable" fractions of metals associated with either iron oxide, manganese oxide or organic phases. S. lancifolia (being most exceptional in ability to translocate trans·lo·cate
1. To change from one place or one position to another; to displace.

2. To transfer a chromosomal segment to a new position; to cause to undergo translocation.
 metals from roots to leaves) and S. alterniflora generally contained higher uptake levels of metals than J. roemerianus with Cd, Cu and Ni bein g most dramatically accumulated. Pb and Zn were less well accumulated. Cr though at high total levels occurred in sediment mostly in forms not readily available to plants and was at very low levels in plant tissues and vividly demonstrates the importance of defining sediment metals in terms of "bioavailable" rather than total amounts as a level most environmentally meaningful.

11:40 TRANSMISSION OF WHITE SPOT VIRUS TO LITOPENEAUS VA NNA NNA National Notary Association (Chatsworth, California)
NNA National Newspaper Association
NNA Nissan North America Inc.
NNA National News Agency (Lebanon)
NNA Nebraska Nurses Association

M. Andres Soto [*], and Jeffrey M. Lotz, Gulf Coast Research Laboratory, Ocean Springs, MS 39566-7000

White spot virus can cause up to 100 % crop mortality on shrimp farms. The transmission coefficient, [beta], derived from a mathematical epidemiology model with the Reed-Frost method of pathogen transmission was used to compare transmission from cannibalism cannibalism (kăn`ĭbəlĭzəm) [Span. caníbal, referring to the Carib], eating of human flesh by other humans.  to transmission from water of white spot virus to Litopeneaus vannamei, The formula for estimating the transmission rate is: [beta] = 1 - exp (([S.sub.1] I [S.sub.o] / [I.sub.o] where, [S.sub.o] and [I.sub.o] are the number of susceptible and infected animals, respectively, at the beginning of the experiment, and [S.sub.1] are the number of susceptible animals at the end of the experiment. Twelve susceptible animals ([S.sub.o]) were placed in each of nine cylindrical tanks (surface area = 1 [m.sub.2). In four tanks, shrimp were allowed to cannibalize can·ni·bal·ize  
v. can·ni·bal·ized, can·ni·bal·iz·ing, can·ni·bal·iz·es
1. To remove serviceable parts from (damaged airplanes, for example) for use in the repair of other equipment of the same
 one dead, infected shrimp, and shrimp in four other tanks were exposed by cohabitation A living arrangement in which an unmarried couple lives together in a long-term relationship that resembles a marriage.

Couples cohabit, rather than marry, for a variety of reasons. They may want to test their compatibility before they commit to a legal union.
 to one infected shrimp. Shrimp in one tank served as the negative control. After 14 hours all shrimp were isolated to avo id further exposure from newly infected shrimp and at day four the experiment was stopped. All shrimp were examined histologically for white spot virus. The transmission coefficient from cannibalism was 0.38, and from water was zero. Partial funding was provided by USDAICSREES grant # 98-38808-6019.
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Publication:Journal of the Mississippi Academy of Sciences
Geographic Code:1USA
Date:Jan 1, 2000

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