Leashing the rattlesnake: a behind-the-scenes look at experimental design.Depending on how you look at them, snakes have no neck or nothing but neck, and either way, Ron Swaisgood had a problem. To finish his Ph.D. at the University of California, Davis The University of California, Davis, commonly known as UC Davis, is one of the ten campuses of the University of California, and was established as the University Farm in 1905. he had to figure out how to put a rattlesnake rattlesnake, poisonous New World snake of the pit viper family, distinguished by a rattle at the end of the tail. The head is triangular, being widened at the base. The rattle is a series of dried, hollow segments of skin, which, when shaken, make a whirring sound. on a leash. Obviously, dropping a slipknot (web) SlipKnot - A graphical World-Wide Web browser specifically designed for Microsoft Windows users who have Unix shell accounts with their service providers. Its primary feature is that it does not require SLIP or PPP or TCP/IP services. around the snake's neck wouldn't do. Swaisgood's research project required that the snake comfortably slither slith·er
v. slith·ered, slith·er·ing, slith·ers
1. To glide or slide like a reptile. See Synonyms at slide.
2. To walk with a sliding or shuffling gait.
3. , coil, and strike but still be tethered Attached to a data or power source by wire or fiber. Contrast with untethered. tightly enough that there was no chance it could escape.
Swaisgood eventually developed a great snake leash, finished his degree, and proceeded to his current job at the San Diego Zoo San Diego Zoo
One of the world's largest collections of mammals, birds, and reptiles, located in San Diego, Calif., and administered by the Zoological Society of San Diego. The 100-acre (40. . The scientific paper based on the research just says he tethered the snake and then goes on to describe the ways that ground squirrels assess snakes as threats (SN: 10/9/99, p. 237). The leash joined thousands of other little unsung triumphs of creativity in experimental design that don't usually show up in scientific articles--or reports in Science News. Ask for the details, though, and another side of research appears. Here, many biologists say, is a lot of the fun.
Experimental science demands creativity in solving problems great and small, and the study of animal behavior makes a dramatic showcase for the process. Animals clearly have minds of their own, and to elucidate such foreign perspectives, researchers have to design cleverly, from the basic strategy of the experiment to dozens of lesser details. The traditions of animal behavior celebrate do-it-yourself flair, and experiments mix the sublimely high tech with the ridiculously simple. Consider the snake leash.
Swaisgood reminisces that the first Northern Pacific rattlesnake he'd caught for his experiment "was kind of a phlegmatic phlegmatic /phleg·mat·ic/ (fleg-mat´ik) of dull and sluggish temperament.
phleg·mat·ic or phleg·mat·i·cal
1. Of or relating to phlegm.
2. snake." The university veterinary surgeons who frequently prepare animals for experiments gave the rattler an implant of a little plastic loop. Swaisgood then just clipped a line to the loop and staked the snake in place.
The next snake, however, had a completely different personality and moved vigorously. A loop implant didn't look like a good idea. "We were concerned he might hurt himself, Swaisgood says. "We started going through our tackle box of research gear looking for Looking for
In the context of general equities, this describing a buy interest in which a dealer is asked to offer stock, often involving a capital commitment. Antithesis of in touch with. something else." He finally leashed his rattlesnake by attaching fishing line to it with--bonus points to readers who saw this coming--that icon of invention: duct tape.
FOOLING MOTHER NATURE The core of an experiment in behavioral science depends on changing one thing about an animal's world but leaving everything else the same. This challenge sometimes demands a mix of scientific sophistication so·phis·ti·cate
v. so·phis·ti·cat·ed, so·phis·ti·cat·ing, so·phis·ti·cates
1. To cause to become less natural, especially to make less naive and more worldly.
2. and parlor tricks.
Ken Kardong couldn't use a silk handkerchief as a blindfold blindfold
worn by personification of justice. [Art: Hall, 183]
See : Justice since the eyes he needed to cover belonged to a rattlesnake. After Kardong's lab at Washington State University Washington State University, at Pullman; land-grant and state supported; chartered 1890, opened 1892 as an agriculture college. From 1905 to 1959 it was the State College of Washington. in Pullman had documented the biomechanical marvels of the snake's strike, he began thinking that the snake's sensory systems needed to be equally marvelous to take advantage of that natural engineering. He decided to study how the snake's senses contributed to strike targeting, so he wanted to block, and then restore, each of its senses.
Snakes' eyes carry a dear, protective layer that lacks the nerve endings that make human eyeballs so tender. "It's like they're wearing glasses," says Kardong. So, with a conventional snake-handling stick, he held a rattler down on a counter, got a firm grip at the back of its head, and placed a patch of black electrical tape over each eye.
Kardong next had to invent another kind of blindfold, this one for the infrared-sensing pits on the snake's face. Depending on the size of the snake, the pair of pockets varies from depressions the size of a pinhead to pits that could hold a BB.
Again, Kardong thought of electrical tape, but he wanted extra insulation to block heat. At first, he considered rolling up little balls of paper. Then, a brainstorm came from a Styrofoam coffee cup. He sliced it into strips and rolled bits between his thumb and forefinger forefinger /fore·fin·ger/ (-fing-ger) index finger; the second finger, counting the thumb as first.
See index finger. until he had the right size for each snake.
Kardong says, "My definition of cleverness includes simple. Blindfolding blindfolding
covering a horse's eyes with a blindfold as a means of restraint. Most horses when blindfolded can be persuaded to load onto trailers which they refuse to do without the blindfold. Of some but more limited use in other species. either the eyes or the pits, by the way, made no difference in the accuracy of a rattlesnake's strike. Sight or heat sensing was sufficient on its own.
Other scientists have also needed to jam and unjam a sensory organ. For instance, Alejandro Purgue invented earmuffs Earmuffs are objects designed to cover a person's ears for protection. They consist of a thermoplastic or metal head-band, that fits over the top of the head, and a pad at each end, to cover the external ears. for bullfrogs.
Now at Cornell University's Laboratory of Ornithology ornithology
Branch of zoology dealing with the study of birds. Early writings on birds were largely anecdotal (including folklore) or practical (e.g., treatises on falconry and game-bird management). in Ithaca, Purgue had been studying the acoustics of North American North American
named after North America.
North American blastomycosis
see North American blastomycosis.
North American cattle tick
see boophilusannulatus. bullfrogs. He began to suspect that the power of the male's commanding "ribit" came not from the throat but from vibrations in ear membranes. Purgne needed to damp and then release the membranes to document any differences in calls.
He tried smearing globs of standard laboratory silicon grease on the membranes to prevent them from vibrating vibrating,
v using quivering hand motions made across the client's body for therapeutic purposes. . However, cleaning grease off the frogs' ears to reverse the effect turned out to be a nuisance. So, Purgue cut little ear pads out of the shock-absorbing foam used for inserts in running shoes. (He bought the foam at a supply company; no shoes were harmed in this experiment.) To link the pads, Purgue wound a tiny, tight coil of piano wire into a spring that would go over the frog's head.
This proved the sticking point of the design. "The angle between their ears was so shallow," says Purgne. "If your head was like that, you'd have trouble keeping your earmuffs on, too" He got a prototype to cover abullfrog's head just long enough for him to admire the effect. "It just looked adorable," he says.
For the actual experiments, Purgue came up with a better solution: tidy devices that stay in place--the researcher's fingertips "Fingertips" is a 1963 number-one hit single recorded live by "Little" Stevie Wonder for Motown's Tamla label. Wonder's first hit single, "Fingertips" was the first live, non-studio recording to reach number-one on the Billboard Pop Singles chart in the United States. .
Purgne's hypothesis indeed proved correct. When he covered the frog's ears, the sound lost its power in midcroak (SN: 1//3/98, p. 12).
THREAT ON WHEELS Scientific trickery Trickery
See also Cunning, Deceit, Humbuggery.
Bunsby, Captain Jack
trapped into marriage by landlady. [Br. Lit.: Dombey and Son]
cheated of bride after lavish wedding preparations. [Span. Lit. often demands a faux animal, and researchers turn puppeteers. One of the more famous of these stand-ins goes by the name RoboBadger. Its creator, Dan Blumstein of the University of California, Los Angeles UCLA comprises the College of Letters and Science (the primary undergraduate college), seven professional schools, and five professional Health Science schools. Since 2001, UCLA has enrolled over 33,000 total students, and that number is steadily rising. , studied alarm calls of marmots and needed something harmless that the rodents would find alarming. Old taxidermy taxidermy (tăk`sĭdûr'mē), process of skinning, preserving, and mounting vertebrate animals so that they still appear lifelike. mounts have a long and distinguished history in the study of animal behavior, but the tricky part comes in revealing the stuffed menace to the creatures under study. It's not going to jog into place by itself, so researchers have an equally long and distinguished history of rigging drop cloths on strings to raise the curtain on the pretend threat.
Blumstein saw a way to render the arrival of his menace, a stuffed badger, a little more realistic. He dismantled a remote control model called Monster Truck and mounted the badger on the wheels and motor. RoboBadger now whirs into view on his own, though Blumstein has to select experimental sites near suitable trails that make good motorized-badger highways.
For a fake aerial predator, Blumstein developed Eagle Knievel, a customized, remote control model glider. "Marmots live in rocky places, and there's never a good place to land a model plane," he sighs. "I've spent days by now gluing him back together."
Lizard specialists like Diana K. Hews take a simpler approach. Based at Indiana State University Indiana State University, main campus at Terre Haute; coeducational; est. 1865 as a normal school, became Indiana State Teachers College in 1929, gained university status in 1965. There is also a campus at Evansville (opened 1965). in Terre Haute, she relies on one animal to provoke specific behaviors from its companions. She attaches a looped string to a fishing pole, lassos a real lizard, and then sets it down where she needs territorial conduct or flirtation. As she stands patiently nearby holding the end of the fishing pole, "they'll display, they'll even mate," Hews says.
Some experimenters fool their subjects with a fake environment rather than a phony or scientist-controlled animal. Paul Switzer of Eastern Illinois University Eastern Illinois University is a state university located in Charleston, Illinois. Institution
Eastern Illinois University has approximately 10,000 undergraduates, 1,700 graduate students, and 2,000 faculty and staff. Admission is selective. in Charleston was investigating whether male dragonflies were more likely to switch to a new territory after a string of mating setbacks. He had to jinx jinx
1. A person or thing that is believed to bring bad luck.
2. A condition or period of bad luck that appears to have been caused by a specific person or thing.
tr.v. a dragonfly's love life without changing its style.
"I was working at my aunt and uncle's farm, and they had plenty of sticks and wire," Switzer says. So, he set out sticks good for egg laying in dragonfly dragonfly, any insect of the order Odonata, which also includes the damselfly. Members of this order are generally large predatory insects and characteristically have chewing mouthparts and four membranous, net-veined wings; they undergo complete metamorphosis. territories along a pond edge and attached them to a handle.
Switzer lurked nearby until a male started to impress a female prospect with a tour of a stick. Then Switzer dunked the stick out of sight. The male dragonflies seemed to search for it, but in the confusion, the females typically flew away. And yes, sexual discouragement did incline a male to seek new ground.
DETAILS, DETAILS Even after the research designer has worked out the central stage magic for an experiment, dozens of other challenges may loom ahead.
Aaron Krochmal, who grew up in New York City New York City: see New York, city.
New York City
City (pop., 2000: 8,008,278), southeastern New York, at the mouth of the Hudson River. The largest city in the U.S. without a lot of personal wildlife experience, recalls that his surprises started when he received the first shipment of his test animals. For his dissertation, he'd devoted months to perfecting a protocol to test rattlesnakes' perception of environmental hiding spots, but he'd never had to manage a live rattler.
Disturbingly, his first shipment didn't arrive as he expected, with snakes in a bag, but with each coiled in a 2-pound deli container. Krochmal says, "My first thought was, 'How did they get them in there?' Which was followed rapidly by, 'How am I going to get them out?'"
He'd practiced basic handling routines on nonvenomous snakes, but not extraction methods from deli containers.
To anyone else who should suddenly confront this dilemma, Krochmal, now at Whitman College in Walla Walla, Wash., passes along his solution: Put the snakes in the fridge to chill them to sluggishness. Then, weigh down the top of a container--a length of rebar re·bar
1. A rod or bar used for reinforcement in concrete or asphalt pourings.
2. A group of such rods forming a grid.
[re(inforcing) bar.] does nicely--while working loose the seal. With very long-handled clamps, move the container to the snake's new home, turn the container sideways, and gently squeeze.
Just keeping animals of unfamiliar species healthy in the laboratory can bring its own puzzles, says Hews. She recalls one husbandry crisis when she began rearing lots of little lizards in isolation containers. She fed them mutant fruit flies that can't fly, the cuisine that lizards usually enjoyed in her lab. In the solitary containers, though, many of the fruit flies evaded capture by roosting out of reach on the upper walls or outright escaping.
Hews therefore made her containers roost proof by dipping their upper sections in a nonstick non·stick
Permitting easy removal of adherent food particles: a frying pan with a nonstick surface.
Adjective coating that comes as a liquid.
Will Mackin of the University of North Carolina at Chapel Hill The University of North Carolina at Chapel Hill is a public, coeducational, research university located in Chapel Hill, North Carolina, United States. Also known as The University of North Carolina, Carolina, North Carolina, or simply UNC found a sweet way to measure how deep a bird called a shearwater shearwater, common name for members of the family Procellariidae, gull-like sea birds related to the petrel and the albatross and including the fulmar. Shearwaters are found on unfrozen saltwaters all over the world, with 35 species in North America. dives. After finding the serious electronic wizardry wiz·ard·ry
n. pl. wiz·ard·ries
1. The art, skill, or practice of a wizard; sorcery.
a. A power or effect that appears magical by its capacity to transform: that's available to be far too expensive, he came across journal articles describing equipment that costs about a dime. Mackin subsequently updated the technique. In his experiment, he sucked up confectioners' sugar to dust the insides of thin plastic tubes and then used a cigarette lighter to close one end. He attached the tube to a shearwater, and when the bird dove to forage, the increasing pressure drove water into the tube.
When Mackin recovered the tubes, he checked the ring marking the lowest point that water had dissolved the sugar and from that he could calculate the lowest point of the dive. He learned that the birds routinely dive about 7 meters.
Andrew Mason also figured out a modern version of old equipment as he monitored an Ormia fly's tracking the direction of a sound. Decades ago, German researchers who wanted to monitor the movement of an insect used a globe made for a streetlight. An optical sensor detected when an insect on top of the globe moved off center, and gearing underneath shifted the globe to keep the insect scrabbling on top. That system inspired Mason to paint irregular dots on a Ping-Pong ball and rest it in a modified cradle from an optical computer mouse. When he tethered the fly on top, the optical sensors picked up shifting dots and sent the information directly to a computer.
WHO'S WHO? A basic question in studying social behavior is how to distinguish among the research animals. Even time-tested marking systems, such as colored bands for birds, can turn unexpectedly tricky. The commercial bands don't work on birds such as red-winged blackbirds, says Ken Yasukawa of Beloit College in Wisconsin. The birds fidget fidg·et
v. fidg·et·ed, fidg·et·ing, fidg·ets
1. To behave or move nervously or restlessly.
2. with the bands, squeezing them in their beaks until the bands eventually break off.
An idea from a colleague who custom-made bands for smaller birds, sent Yasukawa off to a craft store. He made his birds ankle bracelets, literally, from the plastic beads that melt together into colorful jewelry when pressed with an iron. He cuts a slit in the ring and pries pries 1
Third person singular present tense of pry1.
Plural of pry1. it open just enough to slip it onto a bird's leg.
Yasukawa says a lot of his scientific equipment has come from Kmart, Ace Hardware, and craft stores, and that a good grasp of their offerings enhances creativity in scientific design. "Sometimes, I go just to look around," he says.
Other experimental behaviorists give close consideration to beauty products. Jill Mateo of the University of Chicago says that she's happy with the blue-black Lady Clairol for dyeing individual marks on the Belding's ground squirrels she studies.
Blumstein, however, has switched to a cattle dye for marking wild marmots. It's great, he says--easy to apply and long-lasting. Unfortunately, it dyes researchers' hands, too, a drawback when his field crews go into town for some sociability.
But that's not the most embarrassing social situation an innovative researcher has faced. Consider a string of experiments designed to see whether nonhuman primates have a sense of self. The researchers dyed a few pink streaks on an animal's ear or eyebrow and gave it a mirror. Apes appeared to notice something amiss, but experiments had not found similar reactions in monkeys. Marc Hauser of Harvard University wondered whether somehow changing the dye protocol, using more or different colors, might lead to different results.
He asked a student in his lab, whose hair on that day was orange, to recommend a nontoxic dye with lots of colors. "Manic Panic," she told him. The best local source turned out to be Hubba-Hubba, a store that advertises itself as "a boudoir of sin."
When Hauser bought four colors of dye, the clerk asked what he was going to do. He told her that they were for animals but immediately regretted the remark.
Back at the lab, Hauser found that he needed even more colors. When he arrived at Hubba-Hubba the next day, the clerk remembered him. "You were just in here yesterday," she said. "Now, what do you really want?"
To prepare budding young scientists for such eventualities, Steve Nowicki of Duke University in Durham, N.C. starts raising the theme of experimental ingenuity in introductory biology courses. Plenty of concepts abound for great experiments, he tells his students, but they can lie around for decades stalled by some obstacle.
"The idea was there--what you had to do was make it work," he says.