Lack of evidence for contact sensitization by Pfiesteria Extract.
BACKGROUND: Members of the estuarine dinoflagellate dinoflagellate
Any of numerous one-celled, aquatic organisms that have two dissimilar flagella and characteristics of both plants (algae) and animals (protozoans). Most are microscopic and marine. genus Pfiesteria are reported to have been responsible for massive fish kills in the southeastern United States. Some reports suggest that exposure to waters having Pfiesteria blooms or occupation-related exposure might result in Pfiesteriainduced dermal irritation and inflammation. Although the toxin has not been isolated and purified, the original data suggested both hydrophilic hydrophilic /hy·dro·phil·ic/ (-fil´ik) readily absorbing moisture; hygroscopic; having strongly polar groups that readily interact with water.
adj. and hydrophobic toxic components. Some investigators propose that dermonecrotic properties are associated with a hydrophobic fraction.
OBJECTIVES: A bioactive C18-bound putative toxin (CPE (Customer Premises Equipment) Communications equipment that resides on the customer's premises.
CPE - Customer Premises Equipment ) extracted from Pfiesteria-laden aquarium water during active fish-killing conditions was examined in the present study to evaluate its potential to produce inflammation and dermal sensitization sensitization /sen·si·ti·za·tion/ (sen?si-ti-za´shun)
1. administration of an antigen to induce a primary immune response.
2. exposure to allergen that results in the development of hypersensitivity. and to determine whether the inflammation and dermatitis reported in early human exposure studies were allergic or irritant in nature.
RESULTS: This fraction was cytotoxic to mouse Neuro-2A cells and primary human epidermal keratinocytes Keratinocytes
Cells found in the epidermis. The keratinocytes at the outer surface of the epidermis are dead and form a tough protective layer. The cells underneath divide to replenish the supply. (NHEK NHEK Normal Human Epidermal Keratinocyte ) at a concentration of 1 mg/mL. Balb/C mice exposed to 50-200% CPE by skin painting exhibited a 6-10% increase in ear swelling relative to vehicle-treated mice in a primary irritancy assay. There was no increase in lymph node cell proliferation as measured using the local lymph node assay. Exposure to CPE in culture up-regulated interleukin-8 in NHEK, whereas granulocyte granulocyte /gran·u·lo·cyte/ (gran´u-lo-sit?) granular leukocyte.granulocyt´ic
band-form granulocyte band cell.
n. macrophage--colony--stimulating factor and tumor necrosis factor tumor necrosis factor
n. Abbr. TNF
A protein that is produced in the presence of an endotoxin, especially by monocytes and macrophages, is able to attack and destroy tumor cells, and exacerbates chronic inflammatory diseases. [alpha] were only minimally altered.
CONCLUSIONS: This study suggests that CPE is cytotoxic to keratinocytes in culture at high concentrations and that it induces mild, localized irritation but not dermal sensitization.
KEY WORDS: allergic contact dermatitis allergic contact dermatitis Allergic dermatitis Dermatology A condition caused by cell-mediated immunity due to contact with haptens–eg, nickel, chromates, ursodiols in poison ivy and poison oak, synthetic chemicals, drugs, cosmetics, jewelry, neomycin , contact irritant dermatitis, cytokines, delayed-type hypersensitivity, keratinocytes, Pfiesteria, sensitization. Environ Health Perspect 115:1023-1028 (2007). doi:10.1289/ehp.9559 available via http://dx.doi.org/ [Online 26 February 2007]
Members of the estuarine dinoflagellate genus Pfiesteria are reported to have been responsible for massive fish kills between 1991 and 1998, primarily in the estuaries of North Carolina and Maryland. The classic signs of Pfiesteria poisoning in fish are skin ulcerations Ulcerations
Breaks in skin or mucous membranes that are often accompanied by loss of tissue on the surface.
Mentioned in: Hypersplenism and altered, erratic swimming patterns (Burkholder et al. 1992; Glasgow et al. 1995). The etiology of the skin ulcers has yet to be determined. Several theories have been proposed, including a) that Pfiesteria complex organisms produce a potent exotoxin exotoxin /exo·tox·in/ (ek´so-tok?sin) a potent toxin formed and excreted by the bacterial cell, and free in the surrounding medium. that destroys the integrity of the skin directly (Glasgow et al. 1995) or induces necrolysis followed by opportunistic microbial infection (Burkholder 1999; Noga et al. 1996); and b) that the dinoflagellate directly attaches to and feeds on fish epidermis (Drgon et al. 2005; Vogelbein et al. 2002). Another hypothesis is that stress associated with environmental factors such as a Pfiesteria bloom causes severe physiologic dysfunction that can induce the acute ulceration response (Udomkusonsri and Noga 2005) and indirectly lead to pathogenic ulceration by water molds. Other environmental parameters may also induce the initial lesion that subsequently becomes infected by water molds (Kiryu et al. 2002; Noga et al. 1996; Udomkusonsri and Noga 2005).
The Centers for Disease Control and Prevention Centers for Disease Control and Prevention (CDC), agency of the U.S. Public Health Service since 1973, with headquarters in Atlanta; it was established in 1946 as the Communicable Disease Center. (CDC See Control Data, century date change and Back Orifice.
CDC - Control Data Corporation ) includes Pfiesteria-related illness in a multiple system syndrome known as possible estuary-associated syndrome (PEAS) (Samet et al. 2001). Under the PEAS criteria, definitive exposure to estuarine water bearing pathogenic Pfiesteria must be established in concert with the development of specific symptoms. Although there has been no indication of toxicity from consumption of seafood from Pfiesteria-contaminated waters (Golub et al. 1998), intense occupational exposure to active fish-killing Pfiesteria species in a laboratory environment or during a large fish kill event has been reported to induce headache, disruptions in cognitive function, and skin lesions (Burkholder 1999; Glasgow et al. 1995; Grattan et al. 1998). Initial anecdotal reports suggested that people who had extensive environmental exposure to Pfiesteria-contaminated water or aerosols during occupational or recreational aquatic activities might exhibit flulike symptoms, malaise, and respiratory and dermal irritation and inflammation (Grattan et al. 1998; Lowitt and Kauffman 1998; Shoemaker 1997, 1998). The irritation usually resolved spontaneously or by washing with fresh water; several investigators suggested that the lesions might be due to an allergic or toxic reaction (Grattan et al. 1998; Lowitt and Kauffman 1998). In these exposed individuals, immune, liver and renal function profiles, blood chemistries, and blood counts were normal (Grattan et al. 1998). These initial reports of human exposure have been criticized because of the limited number of exposed individuals, the lack of preexposure data and proper controls, the inability to definitively measure toxin exposure, the lack of an environmental screening test for the toxin, the transient nature of the organism, and confounding medical and lifestyle factors of many of those evaluated (Haselow et al. 2001; Moe et al. 2001; Samet et al. 2001). More recently, the CDC-sponsored, 4-year, cross-sectional studies in Maryland, Virginia, and North Carolina found that routine occupational exposure to estuarine environments containing Pfiesteria did not pose any significant neuropsychologic health risk (Moe et al. 2001; Morris et al. 2006). Evaluation of the health and cognitive function of study participants in both the preliminary studies and in the CDC studies attributed the majority of cases to chronic irritation and trauma associated with fishing and crabbing work, estuarine microbes, allergens such as ragweed ragweed, any plant of the genus Ambrosia, coarse, weedy herbs belonging to the family Asteraceae (aster family), most of which are native to America. They have inconspicuous greenish flowers and soft subdivided leaves. or pollen, intense sun and water exposures, and preexisting pre·ex·ist or pre-ex·ist
v. pre·ex·ist·ed, pre·ex·ist·ing, pre·ex·ists
To exist before (something); precede: Dinosaurs preexisted humans.
v.intr. medical and neuropsychologic conditions (Burke and Tester 2002; Collier and Burke 2002; Haselow et al. 2001; Lowitt and Kauffman 1998). In two Maryland studies a small number of skin lesions (seven biopsies from six patients and eight biopsies from four patients) were not ascribed to a specific, alternate diagnosis. Those lesions were biopsied and exhibited either nonspecific chronic inflammatory responses or an infiltrate containing eosinophils Eosinophils
A leukocyte with coarse, round granules present.
Mentioned in: Histiocytosis X
eosinophils and lymphocytes (Grattan et al. 1998; Lowitt and Kauffman 1998). The nondiagnostic lesions and dermal irritation that included erythema erythema (ĕr'əthē`mə), more or less diffuse redness of the skin due to concentration of an abnormally large amount of blood within the small vessels of the skin (hyperemia), as in burns. and an itching, burning sensation experienced by many people in multiple studies were deemed significant in reviews by scientists not associated with the Maryland studies (Burke and Tester 2002; Collier and Burke 2002).
The exact cause of Pfiesteria toxicity is still unknown, although numerous efforts have been made to isolate the toxin and characterize the chemical structure and mechanism of action (Drgon et al. 2005; Moeller et al. 2001). Cell extracts showed very weak activity in a panel of bioassays relative to extracts from Pfiesteria-laden aquarium water, indicating that the putative toxin(s) is preferentially released from the cells (Moeller et al. 2001). Available data suggest both a hydrophilic, neurotoxic neurotoxic
pertaining to or emanating from a neurotoxin.
a case of poisoning by a neurotoxin.
neurotoxic adjective component and a hydrophobic, dermonecrotic component (Samet et al. 2001), although the relative toxicities of the components have been contested (Moeller et al. 2001). A series of studies conducted at Duke University in Durham, North Carolina Durham is a city in the U.S. state of North Carolina. It is the county seat of Durham CountyGR6 and is the fourth-largest city in the state by population. , indicated that the neurotoxic component might induce subtle, specific deficits in the acquisition phase of learning and habituation to the environment in Sprague-Dawley rats but does not affect other neurologic, hematologic hematological, hematologic
pertaining to or emanating from blood cells.
total and differential white cell counts, hematocrit estimation, erythrocyte count. , or histopathologic parameters (Duncan et al. 2005; Levin et al. 1997, 2003; Rezvani et al. 2001). The toxic effects diminished with storage time of the water, suggesting that the putative toxin degrades quickly (Levin et al. 1997), possibly because of oxidative degradation (Moeller et al. 2001).
Earlier studies have associated intense exposure to Pfiesteria with a variety of ailments (Burkholder 1999; Glasgow et al. 1995; Grattan et al. 1998; Lowitt and Kaufman 1998; Shoemaker 1997, 1998). The eosinophilic eosinophilic /eo·sin·o·phil·ic/ (-fil´ik)
1. readily stainable with eosin.
2. pertaining to eosinophils.
3. pertaining to or characterized by eosinophilia. infiltration of dermal lesions, inflammation, and burning sensation reported in people exposed to Pfiesteria-contaminated waters suggest potential allergic or irritant dermatitis. Both allergic contact dermatitis and contact irritant dermatitis are mediated by keratinocyte-derived cytokines (Corsini and Galli 2000). The goal of our present investigation was to use physical responses and cytokine profiles to assess the potential of a C18-bound fraction of Pfiesteria extract (CPE) as an allergen and contact sensitizer sensitizer
see antigen. and to distinguish a possible local irritancy response from dermal sensitization.
Materials and Methods
Animals and treatment. Female Balb/C mice were obtained from Taconic Farms, Inc. (Rockville, MD) at 6 weeks of age, maintained on a 12-hr light/dark cycle at 20-22[degrees]C, and acclimated for 1 week before treatment. The mice received NIH-31 diet (Ziegler Brothers, Inc., Gardner, PA) and water ad libitum and were monitored daily for acute toxicity and weighed weekly. Treatment had no effect on the body weight of the mice (data not shown). We conducted all experiments under an approved National Institute of Environmental Health Sciences' (NIEHS NIEHS National Institute of Environmental Health Sciences (NIH, DHHS) ) Animal Care and Use Committee protocol. The mice were pathogen free, treated humanely, and housed in an Association for Assessment and Accreditation of Laboratory Animal Care-accredited facility. All experiments using laboratory animals were conducted with regard for alleviation of pain and distress.
Production of Pfiesteria extract. A CPE was produced at North Carolina State University History
A plural of aquarium. . The fish were maintained in 20-L aquaria at room temperature in 20 ppt artificial seawater (Instant Ocean; Aquarium Systems, Mentor, OH) on a 12-hr light:dark cycle in an approved biocontainment facility with four conditioned box filters for aeration aeration /aer·a·tion/ (ar-a´shun)
1. the exchange of carbon dioxide for oxygen by the blood in the lungs.
2. the charging of a liquid with air or gas.
n. and nitrification nitrification /ni·tri·fi·ca·tion/ (ni?tri-fi-ka´shun) the bacterial oxidation of ammonia to nitrite and then to nitrate in the soil.
1. . Water quality was ammonia < 0.002 mg/L, pH 8.0-8.2, salinity 20 ppt, and dissolved oxygen at saturation. The aquarium was inoculated with the algal algal
pertaining to or caused by algae.
is very rare but systemic and udder infections are recorded. See protothecosis.
the algae Prototheca trispora and P. culture, and 8-10Tilapia nilotica 2.5-5 cm in length were added. As fish died, more were added to the aquarium until the bloom had reached a level sufficient to kill 90-100% of all fish within 48 hr. Time to death of the fish was used as the indicator of sample toxicity because it proved to be a better predictor than algal counts. The bioactive material was extracted from the seawater at the peak of the fishkilling event by adsorption to C18 silica gel. The aquarium water was pumped into a separate vessel and 1 g C18 resin (80 [mu]m; Waters Corp., Milford MA) was added for every liter of aquarium water. The resin and aquarium water were reacted together for 40-60 min while the magnetic stir bar slowly mixed the solution to keep the resin suspended. The resin was allowed to settle; the aquarium water was aspirated from the vessel; and the resin was washed with a small volume of clean 20 ppt seawater and transferred to polypropylene tubes. One volume of 40% acetonitrile acetonitrile /ac·e·to·ni·trile/ (as?e-to-ni´tril) a colorless liquid with an etherlike odor used as an extractant, solvent, and intermediate; ingestion or inhalation yields cyanide as a metabolic product. (ACN) was added to the resin (final concentration, 20% ACN), and the resin was stored at 4[degrees]C in the dark under nitrogen until the bioactive fraction was eluted. A 2-mL-packed aliquot aliquot (al-ee-kwoh) adj. a definite fractional share, usually applied when dividing and distributing a dead person's estate or trust assets. (See: share) of the C18 resin with bound test article was poured into a 15-mL Bio-Rad Econo Pac disposable column (Bio-Rad, Hercules, CA); the liquid was drained; and the flowthrough was passed over the resin 5 times. The resin was washed with 20 mL 20% ACN, and the CPE was eluted with 20 mL 100% ACN and dried under nitrogen. The resulting product from 2 mL of packed resin resuspended in 3 mL dimethyl sulfoxide (DMSO DMSO dimethyl sulfoxide.
Dimethyl sulfoxide; a colorless hygroscopic liquid obtained from lignin, used as a penetrant to convey medications into the tissues.
n. ) was defined as 100% CPE for in vivo assays the same quantity of product resuspended in 1.5 mL DMSO was defined as 200%. To preserve maximal potency, aliquots of CPE were dried, stored at -80[degrees]C, and prepared fresh in DMSO or the appropriate dilution solvent for each experiment.
Primary irritancy assay. To establish the minimal irritating and maximal nonirritating concentrations of the CPE for use in sensitization experiments and to ensure that the mice would tolerate multiple days of topical exposure to the ear, we conducted a primary irritancy assay following a modification of the method described by Hayes and Meade (1999). Pretreatment pretreatment,
n the protocols required before beginning therapy, usually of a diagnostic nature; before treatment.
n See predetermination. ear thickness was measured with Oditest (Mitutoyo Co., Kawasaki, Japan) precision calipers, and 25 [mu]L of CPE in DMSO or vehicle (DMSO) was applied to the dorsum dorsum /dor·sum/ (dor´sum) pl. dor´sa [L.]
1. the back.
2. the aspect of an anatomical structure or part corresponding in position to the back; posterior in the human. of each ear for 3 consecutive days (days 1, 2, 3). The mice were rested for 2 days. Ear swelling was measured on day 6. The percent of ear swelling was calculated as follows: [(posttreatment measure/pretreatment measure)x 100]-100. There were five mice per treatment group.
Local lymph node assay (LLNA LLNA Local Lymph Node Assay ). We performed the LLNA according to Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM ICCVAM Interagency Coordination Committee on the Validation of Alternative Methods ) guidelines (Dean et al. 2001). The mice (five per treatment group) were treated with CPE by skin painting as described above. The CPE was dissolved in DMSO, and control mice were treated with DMSO, which is the recommended solvent for water-soluble materials in the LLNA (Ryan et al. 2002). Ten percent hexylcinnamaldehyde (HCA HCA,
n.pr See acid, hydroxycitric. ), the positive control, was also prepared in DMSO. On day 6 the mice were injected intravenously via the tail vein with 20 [micro]Ci of [[methyl-.sup.3]H]-thymidine (specific activity, 6.7 Ci/mmol; NEN/PerkinElmer Life Sciences, Boston, MA) in 250 [micro]L sterile phosphate-buffered saline (PBS PBS
in full Public Broadcasting Service
Private, nonprofit U.S. corporation of public television stations. PBS provides its member stations, which are supported by public funds and private contributions rather than by commercials, with educational, cultural, ). Five hours later the mice were euthanized via carbon dioxide asphyxiation asphyxiation /as·phyx·i·a·tion/ (as-fix?e-a´shun) suffocation; the stoppage of respiration.
Oxygen starvation of tissues. and the cervical (auricular auricular /au·ric·u·lar/ (aw-rik´u-lar)
1. pertaining to an auricle.
2. pertaining to the ear.
1. ) lymph nodes (Dean et al. 2001) excised; the same nodes were removed from each mouse and used in their entirety for analysis. We prepared a single-cell suspension of lymph node cells (LNC) in PBS for each mouse by grinding the lymph nodes with the flat end of a 5-cc syringe. The LNC were washed twice in 10 mL PBS at 4[degrees]C. The cell membranes were ruptured, and the protein-bound DNA DNA: see nucleic acid.
or deoxyribonucleic acid
One of two types of nucleic acid (the other is RNA); a complex organic compound found in all living cells and many viruses. It is the chemical substance of genes. was precipitated with 5% trichloroacetic acid (TCA TCA
1. trichloroacetic acid.
2. tricarboxylic acid cycle (Krebs cycle).
TCA Tricyclic antidepressant, see there ; Sigma Chemical Co., St. Louis, MO) at 4[degrees]C overnight. After centrifugation, the pellet was resuspended in 2 mL 5% TCA and transferred to a scintillation scintillation /scin·til·la·tion/ (sin?ti-la´shun)
1. an emission of sparks.
2. a subjective visual sensation, as of seeing sparks.
3. vial containing 18 mL UltimaGold scintillation fluid (Packard Instrument Co., Meriden, CT). Incorporation of [3H]-thymidine was measured by [beta]-scintillation counting as disintegrations per minute (dpm) for each mouse.
Cell proliferation and viability assays. Primary normal human epidermal keratinocytes (NHEK), obtained from reduction mammoplasty (BioWhittaker, Walkersville, MD), were cultured in serum-free, low calcium (0.15 mM) keratinocyte keratinocyte /ke·rat·i·no·cyte/ (ker-at´in-o-sit) the epidermal cell that synthesizes keratin, known in its successive stages in the layers of the skin as basal cell, prickle cell, and granular cell. basal medium (KBM-2; BioWhittaker) supplemented with epidermal growth factor Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation. Human EGF is a 6045 Da protein with 53 amino acid residues and three intramolecular disulfide bonds. (0.1 ng/mL), bovine pituitary extract (0.4%), insulin (5 [micro]g/mL), hydrocortisone hydrocortisone (hī'drəkôr`tĭzōn'), another name for the steroid hormone cortisol, more especially used to refer to preparations of this hormone used medicinally. (0.5 [micro]g/mL), gentamicin gentamicin /gen·ta·mi·cin/ (jen?tah-mi´sin) an aminoglycoside antibiotic complex isolated from bacteria of the genus Micromonospora, (50 [micro]g/mL), and amphotericin (50 [micro]g/mL) in 96-well plates at 1 x [10.sup.4] cells/well at 37[degrees]C and 5% [CO.sub.2]. Neuro-2A cells [American Type Culture Collection American Type Culture Collection (ATCC) is a private, not-for-profit biological resource center whose mission focuses on the acquisition, authentication, production, preservation, development and distribution of standard reference microorganisms, cell lines and other materials for (ATCC ATCC American Type Culture Collection, see there ), Manassas, VA] were cultured in modified minimal essential medium with Earle's salts and nonessential amino acids (MEM, from ATCC), 10% fetal calf serum, 2 mM glutamine glutamine (gl`təmēn), organic compound, one of the 20 amino acids commonly found in animal proteins. , and (100 U/ 100 [micro]g)/mL penicillin--streptomycin in 96-well plates at 2 x [10.sup.4] cells/well at 37[degrees]C and 5% [CO.sub.2]. Keratinocytes and Neuro-2A cells were allowed to grow in culture for 24 hr, then the medium was removed and appropriate dilutions of CPE in fresh medium were added (n = 6). After 24 hr of exposure to CPE, the medium was removed and frozen at -80[degrees]C for use in cytokine assays. Cell proliferation was measured using the CellTiter 96 [AQ.sub.ueous] One Solution Cell Proliferation Assay according to the manufacturer's instructions (Promega, Madison, WI). Twenty microliters of the reagent 3-(4,5-dimethylthiazol-2-yl)- 5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium salt (MTS) in 100 [micro]L fresh medium was added to each well; the cultures were returned to the incubator; and the absorbance absorbance /ab·sor·bance/ (-sor´bans)
1. in analytical chemistry, a measure of the light that a solution does not transmit compared to a pure solution. Symbol .
2. at 490 nm was measured 1, 2, and 3 hr later using a microplate reader and SoftMax Pro analysis software (Molecular Devices, Sunnyvale, CA). Cell viability was measured in NHEK by neutral red dye uptake. Cells were incubated with 50 [micro]g/mL neutral red dye in 200 [micro]L fresh medium for 3 hr. The medium-containing dye was removed and the cells were fixed for 1 min in 200 [micro]L of fixing solution (0.5% formaldehyde/ 0.1% calcium chloride). The fixing solution was removed, and the dye taken up by viable cells was extracted with 200 [micro]L 50% ethanol/1% acetic acid before absorbance determination at 540 nm, as previously described (Trouba et al. 2002).
Cytokine assays. The levels of selected cytokines in cell culture supernatants from NHEK cultures before the addition of MTS or neutral red reagent (described above) were measured using the Luminex xMAP Protein Immunoassay according to the manufacturer's instructions (Biosource, Camarillo, CA). Briefly, 50 [micro]L of sample was incubated with 50 [micro]L of assay diluent diluent /dil·u·ent/ (dil´oo-int)
1. causing dilution.
2. an agent that dilutes or renders less potent or irritant.
Serving to dilute.
n. and antibody-coated beads for 2 hr at room temperature in the dark with vigorous shaking. After washing, the samples were incubated with 100 [micro]L biotinylated detection antibody for 1 hr, then with 100 [micro]L streptavidin--R-phycoerythrin (RPE) for 30 min. Cytokine bead flourescence was measured and analyzed using the Luminex LabMAPscanner and software (Biosource). Two xMAP kits were used. One kit measured tumor necrosis factor (TNF TNF
tumor necrosis factor
n an abbreviation for tumor
f )-[alpha], granulocyte macrophage--colony--stimulating factor (GMCSF GMCSF
granulocyte-macrophage colony-stimulating factor ), interleukin (IL)-8, IL-1[beta], and IL-6 in supernatants from three different cell culture experiments; the other measured TNF-[alpha], GM-CSF GM-CSF granulocyte-macrophage colony-stimulating factor.
Granulocyte/macrophage colony stimulating factor (GM-CSF)
A substance produced by cells of the immune system that stimulates the attack upon foreign cells. , IL-8, macrophage inflammatory protein This article is about proteins. For the chemical compound CCl4, see Carbon tetrachloride.
Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. (MIP MIP
See: Monthly income preferred security )-[alpha], and MIP-1[beta] from five cell culture experiments. In each experiment, six culture wells were independently treated for each group (n = 6). With the first cytokine kit, each culture was assayed independently, that is, three experiments with six replicates per group per experiment. Expression levels for IL-8, IL-1[beta], and IL-6 were all above the limit of detection (per the manufacturer's specifications) in the first assay. IL-1[beta], and IL-6 data are presented from this assay. Because TNF-[alpha] and GM-CSF expression levels were generally below the limit of detection for the assay, two replicate cultures were pooled and concentrated to increase the possibility of detection, giving three samples per group, that is, five experiments with three replicates per group per experiment. All samples from the first assay were repeated in the second assay. Each experiment was maintained separately and there were matched controls for each experiment. After the assay was completed, the data were pooled for statistical analysis. Data for TNF-[alpha], GM-CSF, IL-8, MIP-1[alpha], and MIP-1[beta] are presented from the second assay.
Analysis. We used statistical tests, including one- or two-way analysis of variance (ANOVA anova
see analysis of variance.
ANOVA Analysis of variance, see there ), Fisher's least significant difference (LSD LSD or lysergic acid diethylamide (lī'sûr`jĭk, dī'ĕth`ələmĭd, dī'ĕthəlăm`ĭd), alkaloid synthesized from lysergic acid, which is found in the fungus ergot ( ) test, and Dunnett's test to determine the significance of the results; we selected a maximal value of p [less than or equal to]?0.05 to determine statistical differences between treatment groups. We used ANOVA to evaluate overall variance. Fisher's LSD is a multiple comparison test that was used to compare treatment groups against all other groups. Dunnett's test was used to compare treatment groups against the assay control.
Validation of CPE potency and cytotoxicity. The effect of CPE on Neuro-2A cell proliferation was measured to evaluate potency of the CPE. Earlier studies established Neuro-2A as a reference cell line that is highly sensitive to similar Pfiesteria extracts (McClellan-Green et al. 1997). The dried CPE was not soluble in water, ACN, or acetone and olive oil 4:1 (AOO), but it formed a suspension at 1 x [10.sup.5 ][micro]g/mL in 100% DMSO, and was soluble in culture following serial dilution to 1 x?[10.sup.3.][micro]g/mL in media containing 1% DMSO. A pilot experiment demonstrated that 1% DMSO, 1% AOO, and 1% ACN did not inhibit cell proliferation in the MTS assay (data not shown). The MTS compound is reduced via NADPH NADPH the reduced form of NADP.
The reduced form of NADP.
reduced form of nicotinamide adenine dinucleotide phosphate (NADP) used in a number of reductive synthesis such as fatty or NADH NADH the reduced form of NAD.
The reduced form of NAD.
n.pr a coenzyme that incorporates niacin and involved in the Krebs cycle. into a soluble, colored formazan product in metabolically active cells; nonproliferating cells cannot affect this reaction. CPE induced cytotoxicity in Neuro-2A cells at 1 x [10.sup.3][micro]g/mL (Figure 1A). Diluted CPE retained this activity when dissolved in ACN, but in AOO activity was notably reduced (Figure 1B). CPE was still cytotoxic to Neuro-2A cells 30 days after reconstitution but had lost some of its potency (data not shown), indicating that biological activity might diminish over time. To preserve full activity of the extract throughout the study, CPE was stored dried at -80[degrees]C and was prepared fresh for each experiment.
The keratinocyte is the predominant cell population in the skin, and the release of keratinocyte--derived, proinflammatory cytokines mediates both irritant and allergic contact dermatitis. Therefore, we evaluated the ability of CPE to induce cytotoxicity in NHEK. Cell viability (Figure 2A) and proliferation (Figure 2B) were both inhibited in primary cells exposed to CPE at 1 x [10.sup.3] [micro]g/mL, the same concentration that was cytotoxic to mouse Neuro-2A cells.
CPE-induced contact irritation and sensitization. Because xenobiotic-induced dermatoses and skin lesions can result from exposure to both sensitizers and irritants, and because epidemiologic studies had suggested both mechanisms as responsible for Pfiesteriainduced skin lesions, we examined CPEinduced dermal irritation and sensitization in mice using the primary irritancy assay and local lymph node assay, respectively. Mice exposed to 50-200% CPE experienced a 6-10% increase in ear swelling relative to DMSO-treated mice (Figure 3A). No increase in lymph node cell proliferation was demonstrated at any concentration of CPE tested (Figure 3B). Mice treated with DMSO without CPE served as the control for statistical comparison. The known sensitizers HCA (figure 3B) and dinitrofluorobenzene (data not shown) induced the expected response when dissolved in DMSO, indicating that activity was not destroyed by the necessary use of DMSO as the vehicle.
CPE-induced alterations in cytokine secretion. Keratinocyte-derived cytokines mediate inflammatory responses in the skin, regardless of whether the stimulus is a result of allergen-specific or irritant effects, therefore, we evaluated cytokine profiles in NHEK following exposure to CPE (Figure 4). At cytotoxic concentrations of CPE (1 x [10.sup.3] [micro]g/mL), keratinocytes consistently secreted elevated levels of IL-8. Secretion of MIP-1[alpha] and MIP-1[beta] was also increased at 1 x 103 [micro]g/mL. Synthesis of TNF-[alpha] was statistically elevated at 1 x 103 [micro]g/mL, although the average absorbance value (8.8 pg/mL) was below the minimum limit of detection for the assay (10 pg/mL). IL-6, GM-CSF, and IL-1[beta]?expression were not significantly altered by exposure to CPE.
In earlier studies, Pfiesteria has been reported to be an extremely toxic marine microbe (Burkholder et al. 1992, 1999; Glasgow et al. 1995); however, recent studies have introduced several competing theories about toxin production and potency, and the mechanism by which the dinoflaggellate kills fish (Berry et al. 2002; Burkholder et al. 2001; Drgon et al. 2005; Moeller et al. 2001; Vogelbein et al. 2002). This study was designed to evaluate the sensitization potential of a CPE extracted during active fish kill conditions (90-100% mortality in 48 hr) associated with a Pfiesteria isolate in a standard fish bioassay, and to determine whether the inflammation and dermatitis reported in early human exposure studies were allergic or irritant in nature. At high concentrations, the CPE was cytotoxic to human keratinocytes, which secrete cytokines that mediate both allergic and irritant dermatitis. However, in vivo exposure of mice to CPE induced mild, localized edema but not lymph node cell proliferation, suggesting that the compound is a mild dermal irritant not a sensitizer. The murine LLNA is a routinely used, ICCVAM-validated, standalone method for allergic contact dermatitis hazard identification (Dean et al. 2001). Baden et al. (2004) conducted an independent evaluation of a C18-bound extract (Nogatoxin) derived from the same source culture used in this study. Consistent with our findings, they demonstrated that Nogatoxin purified using thin-layer chromatography induced skin erosion and ulceration in fish but did not induce clinical signs of toxicity in mice after oral or intraperitoneal exposure. There were no changes in airway responsiveness or inflammatory response in sheep that received Nogatoxin by tracheal instillation, suggesting that the compound is not a respiratory sensitizer. Limited structural analysis demonstrated an absence of aromaticity ar·o·ma·tic·i·ty
n. pl. ar·o·ma·tic·i·ties
Aromatic quality or character, especially the distinctive structure or properties of the aromatic chemical compounds. , countering the argument of phthalate ester contamination (Baden et al. 2004). In this study we found that 20% di(2-ethylhexyl)phthalate did not induce a response in the primary irritancy assay or LLNA, compared with the vehicle control (data not shown).
Allergic contact dermatitis resulting from sensitization to an allergen is a T-lymphocyte--mediated immune response requiring two temporally distinct exposures (induction and elicitation), whereas contact irritant dermatitis is a localized, reversible, inflammatory response induced by primary contact with a chemical or mechanical irritant (Corsini and Galli 2000). Cytokines are ultimate mediators of both responses. The primary irritancy reaction in vivo and the cytokine profile in vitro suggest that the CPE is more likely to be a mild irritant than a sensitizer. Increased levels of IL-8, MIP-1[alpha], and MIP-1[beta], cytokines associated with irritancy and neutrophil chemotaxis chemotaxis: see taxis. (Lee et al. 2000; Welss et al. 2004), were secreted by NHEK cultured in the presence of 1 x [10.sup.3] [micro]g/mL CPE, but TNF-[alpha], GM-CSF, and IL-1[beta], cytokines associated with sensitization (Kimber 1996), were minimally affected.
Cumulative irritant dermatitis, a common nonspecific skin disease, is characterized by erythema, dryness and scaling that are usually observed with repeated exposure to a mixture of weak chemicals (Smith et al. 2002). Several models of irritant-induced inflammation include initial epithelial damage followed by either the release of cytokines by epithelial cells or the direct chemical effect on the underlying, exposed inflammatory cells (Smith et al. 2002; Welss et al. 2004). One plausible model for CPE-induced dermal irritation may be that the lipophilic lipophilic,
adj/n the ability to dissolve or attach to lipids.
adj 1. showing a marked attraction to, or solubility in, lipids.
2. CPE perturbs the lipid bilayer of epithelial cell membranes, altering membrane fluidity and receptor-mediated signal transduction. With this model, IL-1[beta] would be up-regulated and subsequently induce secretion of IL-8, a chemotactic che·mo·tac·tic
Of or relating to chemotaxis. cytokine that recruits neutrophils and lymphocytes to the site of exposure (Welss et al. 2004). Secretion of IL-8 by keratinocytes after exposure to CPE supports this theory. Additionally, the response to neutral red uptake in this study suggests that CPE may damage membrane integrity. Neutral red penetrates the membranes of viable cells by nonionic diffusion and accumulates in the lysosomes lysosomes
n the self-contained organelles found inside most cells, which contain hydrolytic enzymes that aid in intracellular digestion. ; alterations to the cell surface or lysosomal lysosomal
pertaining to or emanating from lysosomes.
enzymes located in the lysosomes.
lysosomal phospholipidosis membranes lead to irreversible damage and decreased dye uptake (NIEHS 2001). Melo et al. (2001) demonstrated that a different Pfiesteria extract preparation altered the permeability of rat pituitary cells.
Both human and animal studies have demonstrated that irritancy can act as an adjuvant to skin sensitization (Smith et al. 2002). At low-to-moderate levels, irritant exposure in conjunction with allergen exposure may lower the threshold for sensitization, increase the frequency of sensitization (both induction and elicitation phases), increase the degree of edema and erythema, and enhance the proliferative response of lymph node cells. The lack of such responses in these studies may result from the absence of co-treatment with a documented sensitizer. Rodent studies using a putative hydrophilic neurotoxin neurotoxin /neu·ro·tox·in/ (noor´o-tok?sin) a substance that is poisonous or destructive to nerve tissue.
See neurolysin. indicated that bioactive extracts from both P. piscicida and P. shumwayae potentiated the learning, memory, attention, and behavior alterations induced by the anticholinergic drug scopolamine scopolamine (skōpŏl`əmēn, –mĭn) or hyoscine (hī`əsēn', –sĭn), alkaloid drug obtained from plants of the nightshade family (Solanaceae), chiefly from henbane, in rats (Duncan et al. 2005). Our results suggest that although transient irritancy induced by exposure to Pfeisteria-laden water might exacerbate the response to a true sensitizer, enhancing the dermatitis, inflammation, and lesions caused by other microorganisms, allergens, and occupational activities, the CPE tested is not capable of inducing dermal sensitization at the concentrations evaluated. These findings are consistent with recent reviews and the CDCsponsored epidemiology studies (Moe et al. 2001; Morris et al. 2006) that attributed reported symptoms to other common causes and concluded that exposure to Pfiesteriacontaining estuarine environments does not pose a significant health risk.
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1. Producing poison or toxic substances.
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NIH - The United States National Institutes of Health. Publ 01-4500. Research Triangle Park Research Triangle Park, research, business, medical, and educational complex situated in central North Carolina. It has an area of 6,900 acres (2,795 hectares) and is 8 × 2 mi (13 × 3 km) in size. Named for the triangle formed by Duke Univ. , NC:National Institute of Environmental Health Sciences The National Institute of Environmental Health Sciences (NIEHS) is one of 27 Institutes and Centers of the National Institutes of Health (NIH),which is a component of the Department of Health and Human Services (DHHS). The Director of the NIEHS is Dr. David A. Schwartz. .
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(1) Toxicology Operations Branch, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services Noun 1. Department of Health and Human Services - the United States federal department that administers all federal programs dealing with health and welfare; created in 1979
Health and Human Services, HHS , Research Triangle Park, North Carolina, USA; (2) Department of Clinical Sciences, North Carolina State University College of Veterinary Medicine, Raleigh, North Carolina For other uses of this name, see Raleigh.
Raleigh (IPA: /ˈrɑli/, ral-ee) is the capital of the State of North Carolina and the county seat of Wake County. , USA
Address correspondence to D. Germolec, Toxicology Operations Branch, NIEHS, P.O. Box 12233, MD EC-03, Research Triangle Park, NC 27709 USA. Telephone: (919) 541-3230. Fax: (919) 541-0870. E-mail: email@example.com
We thank P. McClellan-Green, K. Trouba, J. Doherty, A. Payne, D. Andrews, and S. Madison for scientific advice and assistance, and J. Pritchard and J. Harry for review of this manuscript.
This research was supported in part by the Intramural intramural /in·tra·mu·ral/ (-mu´r'l) within the wall of an organ.
Occurring or situated within the walls of a cavity or organ. Research Program of the NIH, NIEHS, and NIEHS Research and Development contract 273-99-C-0154.
The authors declare they have no competing financial interests.
Received 28 July 2006; accepted 26 February 2007.