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Investigate growth conditions for pathogens.



Eliminating all pathogens from foods, although ideal, is unrealistic in the face of consumer demand for an increased supply of lightly processed foods. In practice, the microbiological quality of the food chain should be improved by rigorously applying properly developed HAACP plans. The aim of scientists at the Ministry of Agriculture, Fisheries and Food The Ministry of Agriculture, Fisheries and Food was a United Kingdom government department created by the Board of Agriculture Act 1889 and at that time called the Board of Agriculture.  (MAFF MAFF

[formerly] Ministry of Agriculture, Fisheries and Food, in the UK. See DEFRA.
, Joint Food Safety and Standards Group, Department of Health, Skipton House, 80 London Rd., London SE1 6LW, England, U.K.) is to understand how environmental conditions and intracellular mechanisms interact to control the growth, survival and, where applicable, the toxin production of bacterial pathogens in food products.

A notion that gained momentum in the past was that populations of C. jejuni under stress gradually lose the ability to grow on agar plates and be detected, but they might grow when consumed and cause illness. This would have serious consequences for the microbiological quality assurance of the food supply. However, no evidence has emerged to support this view.

All the evidence indicates that stressed populations of Campylobacter Campylobacter

Genus of gram-negative spiral-shaped bacteria infecting mammals. Many species, especially C. fetus, cause miscarriage in sheep and cattle. C. jejuni is a common cause of food poisoning. Sources include meats (particularly chicken) and unpasteurized milk.
 spp. are always curable cur·a·ble
adj.
Capable of being cured or healed.
 when they are capable of colonizing a host, and that the loss of culturability always follows the loss of colonizing ability. In addition, various attempts have been made to define food environments that would uncouple toxin production from growth so that even if the growth of a pathogen occurred accidently in a product, the production of toxins would be minimized.

Researchers also have investigated acid-resistant strains of E. coli O157:H7. All forms of E. coli appear to be sensitive to lactic acid at pH 3. They are especially sensitive to a mixture of lactic acid and 5% ethanol. Peptone peptone /pep·tone/ (pep´ton) a derived protein, or a mixture of cleavage products produced by partial hydrolysis of native protein.pepton´ic

pep·tone
n.
 has been able to protect E. coli and S. aureus The aureus (pl. aurei) was a gold coin of ancient Rome valued at 25 silver denarii. The aureus was regularly issued from the 1st century BC to the beginning of the 4th century AD, when it was replaced by the solidus.  from growth inhibition caused by high concentrations of salt. It stimulates the growth of E. coli under acidic conditions.

Scientists also have developed techniques for studying the effects of new treatments on bacterial membrane activity. The use of pulse electric fields causes the bacterial membrane to leak ultraviolet-absorbing material. But the cells themselves survive either uninjured, or they are killed. There is no injured population.

There may be a reason for this. Susceptibility to pulsed high electric fields may correlate with the fluidity of the membrane. Or a microorganism microorganism /mi·cro·or·gan·ism/ (-or´gah-nizm) a microscopic organism; those of medical interest include bacteria, fungi, and protozoa.  in an electric field may act as a resistor and make the cell membrane permeable.

Further information. Stephen Pugh; phone: +44 0207-972-5079; fax: +44 0207-972-6555; URL URL
 in full Uniform Resource Locator

Address of a resource on the Internet. The resource can be any type of file stored on a server, such as a Web page, a text file, a graphics file, or an application program.
: http://www.doh.gsi.gov.uk.
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Publication:Microbial Update International
Article Type:Brief Article
Geographic Code:4EUUK
Date:Jun 1, 2000
Words:406
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